Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide
University Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France
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Sensors 2014, 14(6), 9984-9994; https://doi.org/10.3390/s140609984
Received: 31 March 2014
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Revised: 8 May 2014
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Accepted: 30 May 2014
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Published: 6 June 2014
(This article belongs to the Special Issue State-of-the-Art Sensors Technology in France)
Metalated porphyrins have been described to bind nucleic acids. Additionally, cobalt porphyrins present catalytic properties towards oxygen reduction. In this work, a carboxylic acid-functionalized cobalt porphyrin was physisorbed on reduced graphene oxide, then immobilized on glassy carbon electrodes. The carboxylic groups were used to covalently graft amino-terminated oligonucleotide probes which are complementary to a short microRNA target. It was shown that the catalytic oxygen electroreduction on cobalt porphyrin increases upon hybridization of miRNA strand (“signal-on” response). Current changes are amplified compared to non-catalytic amperometric system. Apart from oxygen, no added reagent is necessary. A limit of detection in the sub-nanomolar range was reached. This approach has never been described in the literature.
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MDPI and ACS Style
De Souza, C.; Zrig, S.; Wang, D.; Pham, M.-C.; Piro, B. Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide. Sensors 2014, 14, 9984-9994. https://doi.org/10.3390/s140609984
AMA Style
De Souza C, Zrig S, Wang D, Pham M-C, Piro B. Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide. Sensors. 2014; 14(6):9984-9994. https://doi.org/10.3390/s140609984
Chicago/Turabian StyleDe Souza, Camille, Samia Zrig, Dengjun Wang, Minh-Chau Pham, and Benoit Piro. 2014. "Electrocatalytic miRNA Detection Using Cobalt Porphyrin-Modified Reduced Graphene Oxide" Sensors 14, no. 6: 9984-9994. https://doi.org/10.3390/s140609984
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