Using Biotinylated Iron-Responsive Element to Analyze the Activity of Iron Regulatory Proteins
Abstract
:1. Introduction
2. Results
2.1. Optimizing the Method of Using Biotinylated IRE to Analyze IRE-Binding Activity
2.2. Utilizing Biotinylated IRE to Analyze IRP Activity under Variable Iron Conditions
2.3. Biotinylated-IRE Probe Revealed a Truncated IRE-Binding IRP2 Isoform
2.4. Dodecyl Sulfate Levels in the Loading Buffer and Denature Temperature on IRP Band Intensity
3. Discussion
4. Materials and Methods
4.1. Cells
4.2. Plasmids and Transfection
4.3. Mice
4.4. Protocol
4.5. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, D.-L.; Ollivierre, H.; Rouault, T.A. Using Biotinylated Iron-Responsive Element to Analyze the Activity of Iron Regulatory Proteins. Int. J. Mol. Sci. 2024, 25, 4852. https://doi.org/10.3390/ijms25094852
Zhang D-L, Ollivierre H, Rouault TA. Using Biotinylated Iron-Responsive Element to Analyze the Activity of Iron Regulatory Proteins. International Journal of Molecular Sciences. 2024; 25(9):4852. https://doi.org/10.3390/ijms25094852
Chicago/Turabian StyleZhang, De-Liang, Hayden Ollivierre, and Tracey A. Rouault. 2024. "Using Biotinylated Iron-Responsive Element to Analyze the Activity of Iron Regulatory Proteins" International Journal of Molecular Sciences 25, no. 9: 4852. https://doi.org/10.3390/ijms25094852