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Volume 24
Issue 3
10.3390/ijms24032297
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Article
Peer-Review Record

A Novel Role of the Two-Component System Response Regulator UvrY in Enterohemorrhagic Escherichia coli O157:H7 Pathogenicity Regulation

Int. J. Mol. Sci. 2023, 24(3), 2297; https://doi.org/10.3390/ijms24032297
by Pan Wu 1,2,†, Qian Wang 1,2,†, Qian Yang 1,2, Xiaohui Feng 1,2, Xingmei Liu 1,2, Hongmin Sun 1,2, Jun Yan 1,2, Chenbo Kang 1,2, Bin Liu 1,2,3, Yutao Liu 1,2,3,* and Bin Yang 1,2,*
Reviewer 1:
Felipe Del Canto
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2023, 24(3), 2297; https://doi.org/10.3390/ijms24032297
Submission received: 12 December 2022 / Revised: 16 January 2023 / Accepted: 20 January 2023 / Published: 24 January 2023
(This article belongs to the Special Issue Pathogenic Escherichia coli: Pathogenesis, Virulence and Control Strategies)

Round 1

Reviewer 1 Report

I have read the article entitled “A novel characterized role of the two-component system response regulator UvrY in enterohemorrhagic Escherichia coli O157:H7 pathogenicity regulation” by Wu et al. The manuscript describes the role of UvrY, the regulator protein of the BarA/UvrY system, in EHEC O157:H7 adherence and colonization capacity. In addition, it was demonstrated that UvrY increase expression of LEE genes by binding to ler promoter and transcriptomic analyses showed significant changes in transcript levels of a wide repertoire of genes when urvY is knocked. I think the manuscript is a solid piece of work.

Comments:

1-      I suggest to remove the word “characterized” from the title.

2-      Line 103. “…the growth curves were measured before and after uvrY deletion” Please rewrite this sentence. I think I got the point, but I guess the experiment was performed at the same time (in parallel) for wild type and mutant strains.

3-      Line 220. Change for “…reduced expression in the uvrY mutant strain”

4-      Line 221. If I understood the message, the sentence would be “…could affect the activity of the ler promoter” (same at line 224)

5-      Figure 4. Please include an SDS-PAGE photograph of the purified UvrY-Hisx6. This would strength the results.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report


Comments for author File: Comments.pdf

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

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