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Article

Pioglitazone-Loaded PLGA Nanoparticles: Towards the Most Reliable Synthesis Method

1
National Enterprise for NanoScience and NanoTechnology (NEST) Laboratory, Scuola Normale Superiore, Piazza San Silvestro 12, I-56127 Pisa, Italy
2
National Enterprise for NanoScience and NanoTechnology (NEST) Laboratory, Istituto Nanoscienze, CNR, Piazza San Silvestro 12, I-56127 Pisa, Italy
*
Authors to whom correspondence should be addressed.
Academic Editors: Ádám Juhász and Edit Csapó
Int. J. Mol. Sci. 2022, 23(5), 2522; https://doi.org/10.3390/ijms23052522
Received: 28 January 2022 / Revised: 22 February 2022 / Accepted: 22 February 2022 / Published: 25 February 2022
Recent findings have proved the benefits of Pioglitazone (PGZ) against atherosclerosis and type 2 diabetes. Since the systematic and controllable release of this drug is of significant importance, encapsulation of this drug in nanoparticles (NPs) can minimize uncontrolled issues. In this context, drug delivery approaches based on several poly(lactic-co-glycolic acid) (PLGA) nanoparticles have been rising in popularity due to their promising capabilities. However, a fully reliable and reproducible synthetic methodology is still lacking. In this work, we present a rational optimization of the most critical formulation parameters for the production of PGZ-loaded PLGA NPs by the single emulsification-solvent evaporation or nanoprecipitation methods. We examined the influence of several variables (e.g., component concentrations, phases ratio, injection flux rate) on the synthesis of the PGZ-NPs. In addition, a comparison of these synthetic methodologies in terms of nanoparticle size, polydispersity index (PDI), zeta potential (ζp), drug loading (DL%), entrapment efficiency (EE%), and stability is offered. According to the higher entrapment efficiency content, enhanced storage time and suitable particle size, the nanoprecipitation approach appears to be the simplest, most rapid and most reliable synthetic pathway for these drug nanocarriers, and we demonstrated a very slow drug release in PBS for the best formulation obtained by this synthesis. View Full-Text
Keywords: pioglitazone; PLGA; polymeric nanoparticles synthesis; nanoprecipitation; single emulsification-solvent evaporation; encapsulation efficiency; drug loading; drug release kinetics pioglitazone; PLGA; polymeric nanoparticles synthesis; nanoprecipitation; single emulsification-solvent evaporation; encapsulation efficiency; drug loading; drug release kinetics
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MDPI and ACS Style

Todaro, B.; Moscardini, A.; Luin, S. Pioglitazone-Loaded PLGA Nanoparticles: Towards the Most Reliable Synthesis Method. Int. J. Mol. Sci. 2022, 23, 2522. https://doi.org/10.3390/ijms23052522

AMA Style

Todaro B, Moscardini A, Luin S. Pioglitazone-Loaded PLGA Nanoparticles: Towards the Most Reliable Synthesis Method. International Journal of Molecular Sciences. 2022; 23(5):2522. https://doi.org/10.3390/ijms23052522

Chicago/Turabian Style

Todaro, Biagio, Aldo Moscardini, and Stefano Luin. 2022. "Pioglitazone-Loaded PLGA Nanoparticles: Towards the Most Reliable Synthesis Method" International Journal of Molecular Sciences 23, no. 5: 2522. https://doi.org/10.3390/ijms23052522

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