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Communication

Functional Characterization of Mechanosensitive Piezo1 Channels in Trigeminal and Somatic Nerves in a Neuron-on-Chip Model

1
A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Neulaniementie 2, 70211 Kuopio, Finland
2
Finnadvance, Aapistie 1, 90220 Oulu, Finland
*
Authors to whom correspondence should be addressed.
Academic Editor: Sang Won Suh
Int. J. Mol. Sci. 2022, 23(3), 1370; https://doi.org/10.3390/ijms23031370
Received: 16 November 2021 / Revised: 20 January 2022 / Accepted: 24 January 2022 / Published: 25 January 2022
(This article belongs to the Special Issue Mechanosensitive Ion Channels in Health and Disease)
Mechanosensitive ion channels, Piezo1 and 2, are activated by pressure and involved in diverse physiological functions, including senses of touch and pain, proprioception and many more. Understanding their function is important for elucidating the mechanosensitive mechanisms of a range of human diseases. Recently, Piezo channels were suggested to be contributors to migraine pain generation. Migraine is typically characterized by allodynia and mechanical hyperalgesia associated with the activation and sensitization of trigeminal ganglion (TG) nerve fibers. Notably, migraine specific medicines are ineffective for other types of pain, suggesting a distinct underlying mechanism. To address, in a straightforward manner, the specificity of the mechanosensitivity of trigeminal vs. somatic nerves, we compared the activity of Piezo1 channels in mouse TG neurons vs. dorsal root ganglia (DRG) neurons. We assessed the functional expression of Piezo1 receptors using a conventional live calcium imaging setup equipped with a multibarrel application system and utilizing a microfluidic chip-based setup. Surprisingly, the TG neurons, despite higher expression of the Piezo1 gene, were less responsive to Piezo1 agonist Yoda1 than the DRG neurons. This difference was more prominent in the chip-based setup, suggesting that certain limitations of the conventional approach, such as turbulence, can be overcome by utilizing microfluidic devices with laminar solution flow. View Full-Text
Keywords: migraine; trigeminal; dorsal root; mechanosensitive receptors; Piezo1; calcium imaging; microfluidics migraine; trigeminal; dorsal root; mechanosensitive receptors; Piezo1; calcium imaging; microfluidics
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MDPI and ACS Style

Mikhailov, N.; Plotnikova, L.; Singh, P.; Giniatullin, R.; Hämäläinen, R.H. Functional Characterization of Mechanosensitive Piezo1 Channels in Trigeminal and Somatic Nerves in a Neuron-on-Chip Model. Int. J. Mol. Sci. 2022, 23, 1370. https://doi.org/10.3390/ijms23031370

AMA Style

Mikhailov N, Plotnikova L, Singh P, Giniatullin R, Hämäläinen RH. Functional Characterization of Mechanosensitive Piezo1 Channels in Trigeminal and Somatic Nerves in a Neuron-on-Chip Model. International Journal of Molecular Sciences. 2022; 23(3):1370. https://doi.org/10.3390/ijms23031370

Chicago/Turabian Style

Mikhailov, Nikita, Lidiia Plotnikova, Prateek Singh, Rashid Giniatullin, and Riikka H. Hämäläinen. 2022. "Functional Characterization of Mechanosensitive Piezo1 Channels in Trigeminal and Somatic Nerves in a Neuron-on-Chip Model" International Journal of Molecular Sciences 23, no. 3: 1370. https://doi.org/10.3390/ijms23031370

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