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Article

Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures

by
Jingru Zhang
,
Haishen Wen
,
Xin Qi
,
Yonghang Zhang
,
Ximeng Dong
,
Kaiqiang Zhang
,
Meizhao Zhang
,
Jifang Li
and
Yun Li
*
Key Laboratory of Mariculture, Ministry of Education (KLMME), Ocean University of China, Qingdao 266003, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(17), 9812; https://doi.org/10.3390/ijms23179812
Submission received: 24 July 2022 / Revised: 16 August 2022 / Accepted: 17 August 2022 / Published: 29 August 2022
(This article belongs to the Special Issue Myoblast and Muscle Cell Genesis and Regeneration)
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Abstract

Temperature strongly modulates muscle development and growth in ectothermic teleosts; however, the underlying mechanisms remain largely unknown. In this study, primary cultures of skeletal muscle cells of Lateolabrax maculatus were conducted and reared at different temperatures (21, 25, and 28 °C) in both the proliferation and differentiation stages. CCK-8, EdU, wound scratch and nuclear fusion index assays revealed that the proliferation, myogenic differentiation, and migration processes of skeletal muscle cells were significantly accelerated as the temperature raises. Based on the GO, GSEA, and WGCNA, higher temperature (28 °C) induced genes involved in HSF1 activation, DNA replication, and ECM organization processes at the proliferation stage, as well as HSF1 activation, calcium activity regulation, myogenic differentiation, and myoblast fusion, and sarcomere assembly processes at the differentiation stage. In contrast, lower temperature (21 °C) increased the expression levels of genes associated with DNA damage, DNA repair and apoptosis processes at the proliferation stage, and cytokine signaling and neutrophil degranulation processes at the differentiation stage. Additionally, we screened several hub genes regulating myogenesis processes. Our results could facilitate the understanding of the regulatory mechanism of temperature on fish skeletal muscle growth and further contribute to utilizing rational management strategies and promoting organism growth and development.
Keywords: Lateolabrax maculatus; skeletal muscle cells; myogenesis; temperatures; primary cell culture; RNA-seq Lateolabrax maculatus; skeletal muscle cells; myogenesis; temperatures; primary cell culture; RNA-seq

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MDPI and ACS Style

Zhang, J.; Wen, H.; Qi, X.; Zhang, Y.; Dong, X.; Zhang, K.; Zhang, M.; Li, J.; Li, Y. Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures. Int. J. Mol. Sci. 2022, 23, 9812. https://doi.org/10.3390/ijms23179812

AMA Style

Zhang J, Wen H, Qi X, Zhang Y, Dong X, Zhang K, Zhang M, Li J, Li Y. Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures. International Journal of Molecular Sciences. 2022; 23(17):9812. https://doi.org/10.3390/ijms23179812

Chicago/Turabian Style

Zhang, Jingru, Haishen Wen, Xin Qi, Yonghang Zhang, Ximeng Dong, Kaiqiang Zhang, Meizhao Zhang, Jifang Li, and Yun Li. 2022. "Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures" International Journal of Molecular Sciences 23, no. 17: 9812. https://doi.org/10.3390/ijms23179812

APA Style

Zhang, J., Wen, H., Qi, X., Zhang, Y., Dong, X., Zhang, K., Zhang, M., Li, J., & Li, Y. (2022). Morphological and Molecular Responses of Lateolabrax maculatus Skeletal Muscle Cells to Different Temperatures. International Journal of Molecular Sciences, 23(17), 9812. https://doi.org/10.3390/ijms23179812

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