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Open AccessArticle

Systemic Administration of G-CSF Accelerates Bone Regeneration and Modulates Mobilization of Progenitor Cells in a Rat Model of Distraction Osteogenesis

by 1,2,3,4, 1,2,3,4,*, 5, 6, 6, 1,2,3,4,† and 1,2,3,4,†
1
ISM, Aix Marseille University, CNRS, 13009 Marseille, France
2
Department of Orthopaedics and Traumatology, Institute for Locomotion, Sainte-Marguerite Hospital, ISM, Aix Marseille University, APHM, CNRS, 13009 Marseille, France
3
Anatomic Laboratory, ISM, Aix Marseille University, CNRS, 13005 Marseille, France
4
Mecabio Platform, ISM, Aix Marseille University, 13009 Marseille, France
5
AMUTICYT, C2VN, INRAE, INSERM, Aix Marseille University, 13005 Marseille, France
6
C2VN, CERIMED, Aix Marseille University, 13005 Marseille, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Jolie Xu and Magali Cucchiarini
Int. J. Mol. Sci. 2021, 22(7), 3505; https://doi.org/10.3390/ijms22073505
Received: 31 January 2021 / Revised: 16 March 2021 / Accepted: 24 March 2021 / Published: 28 March 2021
Granulocyte colony-stimulating factor (G-CSF) was shown to promote bone regeneration and mobilization of vascular and osteogenic progenitor cells. In this study, we investigated the effects of a systemic low dose of G-CSF on both bone consolidation and mobilization of hematopoietic stem/progenitor cells (HSPCs), endothelial progenitor cells (EPCs) and mesenchymal stromal cells (MSCs) in a rat model of distraction osteogenesis (DO). Neovascularization and mineralization were longitudinally monitored using positron emission tomography and planar scintigraphy. Histological analysis was performed and the number of circulating HSPCs, EPCs and MSCs was studied by flow cytometry. Contrary to control group, in the early phase of consolidation, a bony bridge with lower osteoclast activity and a trend of an increase in osteoblast activity were observed in the distracted callus in the G-CSF group, whereas, at the late phase of consolidation, a significantly lower neovascularization was observed. While no difference was observed in the number of circulating EPCs between control and G-CSF groups, the number of MSCs was significantly lower at the end of the latency phase and that of HSPCs was significantly higher 4 days after the bone lengthening. Our results indicate that G-CSF accelerates bone regeneration and modulates mobilization of progenitor cells during DO. View Full-Text
Keywords: neovascularization; endothelial progenitor cells; mesenchymal stromal cells; hematopoietic stem/progenitor cells; bone formation; G-CSF neovascularization; endothelial progenitor cells; mesenchymal stromal cells; hematopoietic stem/progenitor cells; bone formation; G-CSF
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MDPI and ACS Style

Roseren, F.; Pithioux, M.; Robert, S.; Balasse, L.; Guillet, B.; Lamy, E.; Roffino, S. Systemic Administration of G-CSF Accelerates Bone Regeneration and Modulates Mobilization of Progenitor Cells in a Rat Model of Distraction Osteogenesis. Int. J. Mol. Sci. 2021, 22, 3505. https://doi.org/10.3390/ijms22073505

AMA Style

Roseren F, Pithioux M, Robert S, Balasse L, Guillet B, Lamy E, Roffino S. Systemic Administration of G-CSF Accelerates Bone Regeneration and Modulates Mobilization of Progenitor Cells in a Rat Model of Distraction Osteogenesis. International Journal of Molecular Sciences. 2021; 22(7):3505. https://doi.org/10.3390/ijms22073505

Chicago/Turabian Style

Roseren, Flavy; Pithioux, Martine; Robert, Stéphane; Balasse, Laure; Guillet, Benjamin; Lamy, Edouard; Roffino, Sandrine. 2021. "Systemic Administration of G-CSF Accelerates Bone Regeneration and Modulates Mobilization of Progenitor Cells in a Rat Model of Distraction Osteogenesis" Int. J. Mol. Sci. 22, no. 7: 3505. https://doi.org/10.3390/ijms22073505

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