The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales
Abstract
:1. Introduction
2. Results
2.1. Comparative Analysis of sRNAs Transcriptomic Profiles between Virulent and Avirulent Pathotypes
2.2. Validation of Target Genes That Are Differentially Regulated by RNAi
2.3. Generation of Mutant Strains with Altered Expression of the Candidate Genes
2.4. Functional Analysis of the Genes Regulated by RNAi and Their Role in Virulence
3. Discussion
4. Materials and Methods
4.1. Fungal Strains
4.2. RNA Isolation
4.3. RNA-Sequencing and Analysis for Small RNA Production
4.4. Northern Blot Analyses for mRNA and sRNA
4.5. Genetic Transformation
4.6. Virulence Assays
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pérez-Arques, C.; Navarro-Mendoza, M.I.; Murcia, L.; Navarro, E.; Garre, V.; Nicolás, F.E. The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales. Int. J. Mol. Sci. 2021, 22, 2282. https://doi.org/10.3390/ijms22052282
Pérez-Arques C, Navarro-Mendoza MI, Murcia L, Navarro E, Garre V, Nicolás FE. The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales. International Journal of Molecular Sciences. 2021; 22(5):2282. https://doi.org/10.3390/ijms22052282
Chicago/Turabian StylePérez-Arques, Carlos, María Isabel Navarro-Mendoza, Laura Murcia, Eusebio Navarro, Victoriano Garre, and Francisco Esteban Nicolás. 2021. "The RNAi Mechanism Regulates a New Exonuclease Gene Involved in the Virulence of Mucorales" International Journal of Molecular Sciences 22, no. 5: 2282. https://doi.org/10.3390/ijms22052282