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Article

Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus

1
Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
2
Department of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Elqaliobiya, Egypt
3
Center for Infectious Diseases of Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
4
Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Dakahliya, Egypt
5
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
6
Laboratory of Veterinary Anatomy, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan
7
Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, 35-1, Higashi 23 Ban-cho, Towada, Aomori 034-8628, Japan
8
Department of Virology I, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku, Tokyo 162-8640, Japan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Julie Bouckaert, Keijo Viiri and Kati Juuti-Uusitalo
Int. J. Mol. Sci. 2021, 22(19), 10763; https://doi.org/10.3390/ijms221910763
Received: 26 July 2021 / Revised: 7 September 2021 / Accepted: 28 September 2021 / Published: 5 October 2021
(This article belongs to the Special Issue Novel Biorelevant Intestinal Epithelial In Vitro Models)
Various pathogens, such as Ebola virus, Marburg virus, Nipah virus, Hendra virus, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus (MERS-CoV), and SARS-CoV-2, are threatening human health worldwide. The natural hosts of these pathogens are thought to be bats. The rousette bat, a megabat, is thought to be a natural reservoir of filoviruses, including Ebola and Marburg viruses. Additionally, the rousette bat showed a transient infection in the experimental inoculation of SARS-CoV-2. In the current study, we established and characterized intestinal organoids from Leschenault’s rousette, Rousettus leschenaultii. The established organoids successfully recapitulated the characteristics of intestinal epithelial structure and morphology, and the appropriate supplements necessary for long-term stable culture were identified. The organoid showed susceptibility to Pteropine orthoreovirus (PRV) but not to SARS-CoV-2 in experimental inoculation. This is the first report of the establishment of an expandable organoid culture system of the rousette bat intestinal organoid and its sensitivity to bat-associated viruses, PRV and SARS-CoV-2. This organoid is a useful tool for the elucidation of tolerance mechanisms of the emerging rousette bat-associated viruses such as Ebola and Marburg virus. View Full-Text
Keywords: bat; natural host; organoid; long-term stable culture; virus susceptibility; SARS-CoV-2; ACE2; TMPRSS2 bat; natural host; organoid; long-term stable culture; virus susceptibility; SARS-CoV-2; ACE2; TMPRSS2
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MDPI and ACS Style

Elbadawy, M.; Kato, Y.; Saito, N.; Hayashi, K.; Abugomaa, A.; Kobayashi, M.; Yoshida, T.; Shibutani, M.; Kaneda, M.; Yamawaki, H.; Mizutani, T.; Lim, C.-K.; Saijo, M.; Sasaki, K.; Usui, T.; Omatsu, T. Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus. Int. J. Mol. Sci. 2021, 22, 10763. https://doi.org/10.3390/ijms221910763

AMA Style

Elbadawy M, Kato Y, Saito N, Hayashi K, Abugomaa A, Kobayashi M, Yoshida T, Shibutani M, Kaneda M, Yamawaki H, Mizutani T, Lim C-K, Saijo M, Sasaki K, Usui T, Omatsu T. Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus. International Journal of Molecular Sciences. 2021; 22(19):10763. https://doi.org/10.3390/ijms221910763

Chicago/Turabian Style

Elbadawy, Mohamed, Yuki Kato, Nagisa Saito, Kimika Hayashi, Amira Abugomaa, Mio Kobayashi, Toshinori Yoshida, Makoto Shibutani, Masahiro Kaneda, Hideyuki Yamawaki, Tetsuya Mizutani, Chang-Kweng Lim, Masayuki Saijo, Kazuaki Sasaki, Tatsuya Usui, and Tsutomu Omatsu. 2021. "Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus" International Journal of Molecular Sciences 22, no. 19: 10763. https://doi.org/10.3390/ijms221910763

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