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Development of a Novel Nanoarchitecture of the Robust Photosystem I from a Volcanic Microalga Cyanidioschyzon merolae on Single Layer Graphene for Improved Photocurrent Generation

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Solar Fuels Laboratory, Center of New Technologies, University of Warsaw, Banacha 2C, 02-097 Warsaw, Poland
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Department of Gene Function and Phenomics, National Institute of Genetics, Yata 111, Mishima 411-8540, Japan
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Department of Engineering Fundamental Sciences, Faculty of Engineering, Tarsus University, Tarsus 33400, Turkey
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Department of Metabolic Regulation, Faculty of Biology, Institute of Biochemistry, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland
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Faculty of Physics, University of Warsaw, Pasteura 5, 02-093 Warsaw, Poland
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Cryomicroscopy and Electron Diffraction Core Facility, Center of New Technologies, University of Warsaw, 02-097 Warsaw, Poland
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Department of Biomedical Engineering, Pamukkale University, Denizli 20070, Turkey
*
Author to whom correspondence should be addressed.
Academic Editor: Abderrazzak Douhal
Int. J. Mol. Sci. 2021, 22(16), 8396; https://doi.org/10.3390/ijms22168396
Received: 14 June 2021 / Revised: 22 July 2021 / Accepted: 2 August 2021 / Published: 5 August 2021
(This article belongs to the Special Issue Biomolecular and Biohybrid Systems for Solar Energy Conversion)
Here, we report the development of a novel photoactive biomolecular nanoarchitecture based on the genetically engineered extremophilic photosystem I (PSI) biophotocatalyst interfaced with a single layer graphene via pyrene-nitrilotriacetic acid self-assembled monolayer (SAM). For the oriented and stable immobilization of the PSI biophotocatalyst, an His6-tag was genetically engineered at the N-terminus of the stromal PsaD subunit of PSI, allowing for the preferential binding of this photoactive complex with its reducing side towards the graphene monolayer. This approach yielded a novel robust and ordered nanoarchitecture designed to generate an efficient direct electron transfer pathway between graphene, the metal redox center in the organic SAM and the photo-oxidized PSI biocatalyst. The nanosystem yielded an overall current output of 16.5 µA·cm−2 for the nickel- and 17.3 µA·cm−2 for the cobalt-based nanoassemblies, and was stable for at least 1 h of continuous standard illumination. The novel green nanosystem described in this work carries the high potential for future applications due to its robustness, highly ordered and simple architecture characterized by the high biophotocatalyst loading as well as simplicity of manufacturing. View Full-Text
Keywords: biohybrid nanodevices; biophotovoltaics; Cyanidioschyzon merolae; direct electron transfer; photosystem I; single layer graphene biohybrid nanodevices; biophotovoltaics; Cyanidioschyzon merolae; direct electron transfer; photosystem I; single layer graphene
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MDPI and ACS Style

Izzo, M.; Jacquet, M.; Fujiwara, T.; Harputlu, E.; Mazur, R.; Wróbel, P.; Góral, T.; Unlu, C.G.; Ocakoglu, K.; Miyagishima, S.; Kargul, J. Development of a Novel Nanoarchitecture of the Robust Photosystem I from a Volcanic Microalga Cyanidioschyzon merolae on Single Layer Graphene for Improved Photocurrent Generation. Int. J. Mol. Sci. 2021, 22, 8396. https://doi.org/10.3390/ijms22168396

AMA Style

Izzo M, Jacquet M, Fujiwara T, Harputlu E, Mazur R, Wróbel P, Góral T, Unlu CG, Ocakoglu K, Miyagishima S, Kargul J. Development of a Novel Nanoarchitecture of the Robust Photosystem I from a Volcanic Microalga Cyanidioschyzon merolae on Single Layer Graphene for Improved Photocurrent Generation. International Journal of Molecular Sciences. 2021; 22(16):8396. https://doi.org/10.3390/ijms22168396

Chicago/Turabian Style

Izzo, Miriam, Margot Jacquet, Takayuki Fujiwara, Ersan Harputlu, Radosław Mazur, Piotr Wróbel, Tomasz Góral, C. G. Unlu, Kasim Ocakoglu, Shinya Miyagishima, and Joanna Kargul. 2021. "Development of a Novel Nanoarchitecture of the Robust Photosystem I from a Volcanic Microalga Cyanidioschyzon merolae on Single Layer Graphene for Improved Photocurrent Generation" International Journal of Molecular Sciences 22, no. 16: 8396. https://doi.org/10.3390/ijms22168396

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