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Article

YB-1 Is Altered in Pregnancy-Associated Disorders and Affects Trophoblast in Vitro Properties via Alternation of Multiple Molecular Traits

1
Department of Environmental Immunology, Helmholtz-Centre for Environmental Research-UFZ-, 04318 Leipzig, Germany
2
Clinic of Nephrology and Hypertension, Diabetes and Endocrinology, Otto-von-Guericke University, 39120 Magdeburg, Germany
3
Medical Faculty, Otto-von-Guericke University, 39120 Magdeburg, Germany
4
Perinatal Immunology, Saxonian Incubator for Clinical Translation, Medical Faculty, University of Leipzig, 04103 Leipzig, Germany
*
Authors to whom correspondence should be addressed.
Academic Editors: Hiten D. Mistry and Eun Lee
Int. J. Mol. Sci. 2021, 22(13), 7226; https://doi.org/10.3390/ijms22137226
Received: 4 June 2021 / Revised: 28 June 2021 / Accepted: 28 June 2021 / Published: 5 July 2021
(This article belongs to the Special Issue Placental Related Disorders of Pregnancy)
Cold shock Y-box binding protein-1 (YB-1) coordinates several molecular processes between the nucleus and the cytoplasm and plays a crucial role in cell function. Moreover, it is involved in cancer progression, invasion, and metastasis. As trophoblast cells share similar characteristics with cancer cells, we hypothesized that YB-1 might also be necessary for trophoblast functionality. In samples of patients with intrauterine growth restriction, YB-1 mRNA levels were decreased, while they were increased in preeclampsia and unchanged in spontaneous abortions when compared to normal pregnant controls. Studies with overexpression and downregulation of YB-1 were performed to assess the key trophoblast processes in two trophoblast cell lines HTR8/SVneo and JEG3. Overexpression of YB-1 or exposure of trophoblast cells to recombinant YB-1 caused enhanced proliferation, while knockdown of YB-1 lead to proliferative disadvantage in JEG3 or HTR8/SVneo cells. The invasion and migration properties were affected at different degrees among the trophoblast cell lines. Trophoblast expression of genes mediating migration, invasion, apoptosis, and inflammation was altered upon YB-1 downregulation. Moreover, IL-6 secretion was excessively increased in HTR8/SVneo. Ultimately, YB-1 directly binds to NF-κB enhancer mark in HTR8/SVneo cells. Our data show that YB-1 protein is important for trophoblast cell functioning and, when downregulated, leads to trophoblast disadvantage that at least in part is mediated by NF-κB. View Full-Text
Keywords: cold shock protein; intrauterine growth restriction; preeclampsia; placentation; apoptosis; NF-κB cold shock protein; intrauterine growth restriction; preeclampsia; placentation; apoptosis; NF-κB
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MDPI and ACS Style

Stojanovska, V.; Shah, A.; Woidacki, K.; Fischer, F.; Bauer, M.; Lindquist, J.A.; Mertens, P.R.; Zenclussen, A.C. YB-1 Is Altered in Pregnancy-Associated Disorders and Affects Trophoblast in Vitro Properties via Alternation of Multiple Molecular Traits. Int. J. Mol. Sci. 2021, 22, 7226. https://doi.org/10.3390/ijms22137226

AMA Style

Stojanovska V, Shah A, Woidacki K, Fischer F, Bauer M, Lindquist JA, Mertens PR, Zenclussen AC. YB-1 Is Altered in Pregnancy-Associated Disorders and Affects Trophoblast in Vitro Properties via Alternation of Multiple Molecular Traits. International Journal of Molecular Sciences. 2021; 22(13):7226. https://doi.org/10.3390/ijms22137226

Chicago/Turabian Style

Stojanovska, Violeta, Aneri Shah, Katja Woidacki, Florence Fischer, Mario Bauer, Jonathan A. Lindquist, Peter R. Mertens, and Ana C. Zenclussen. 2021. "YB-1 Is Altered in Pregnancy-Associated Disorders and Affects Trophoblast in Vitro Properties via Alternation of Multiple Molecular Traits" International Journal of Molecular Sciences 22, no. 13: 7226. https://doi.org/10.3390/ijms22137226

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