Next Article in Journal
Bruton’s Tyrosine Kinase Targeting in Multiple Myeloma
Next Article in Special Issue
Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication
Previous Article in Journal
Combination Treatment of OSI-906 with Aurora B Inhibitor Reduces Cell Viability via Cyclin B1 Degradation-Induced Mitotic Slippage
 
 
Article

Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice

1
College of Optometry, University of Houston, Houston, TX 77204, USA
2
School of Biosciences, Cardiff University, Cardiff CF10 3AT, UK
3
Optimvia, Batavia, OH 45103, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Chiara Schiraldi, Donatella Cimini and Annalisa La Gatta
Int. J. Mol. Sci. 2021, 22(11), 5708; https://doi.org/10.3390/ijms22115708
Received: 28 April 2021 / Revised: 24 May 2021 / Accepted: 25 May 2021 / Published: 27 May 2021
(This article belongs to the Special Issue Glycosaminoglycans)
Corneal transparency relies on the precise arrangement and orientation of collagen fibrils, made of mostly Type I and V collagen fibrils and proteoglycans (PGs). PGs are essential for correct collagen fibrillogenesis and maintaining corneal homeostasis. We investigated the spatial and temporal distribution of glycosaminoglycans (GAGs) and PGs after a chemical injury. The chemical composition of chondroitin sulfate (CS)/dermatan sulfate (DS) and heparan sulfate (HS) were characterized in mouse corneas 5 and 14 days after alkali burn (AB), and compared to uninjured corneas. The expression profile and corneal distribution of CS/DSPGs and keratan sulfate (KS) PGs were also analyzed. We found a significant overall increase in CS after AB, with an increase in sulfated forms of CS and a decrease in lesser sulfated forms of CS. Expression of the CSPGs biglycan and versican was increased after AB, while decorin expression was decreased. We also found an increase in KS expression 14 days after AB, with an increase in lumican and mimecan expression, and a decrease in keratocan expression. No significant changes in HS composition were noted after AB. Taken together, our study reveals significant changes in the composition of the extracellular matrix following a corneal chemical injury. View Full-Text
Keywords: glycosaminoglycans; proteoglycans; corneal stroma; scar formation; corneal wounding glycosaminoglycans; proteoglycans; corneal stroma; scar formation; corneal wounding
Show Figures

Figure 1

MDPI and ACS Style

Mutoji, K.N.; Sun, M.; Elliott, G.; Moreno, I.Y.; Hughes, C.; Gesteira, T.F.; Coulson-Thomas, V.J. Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice. Int. J. Mol. Sci. 2021, 22, 5708. https://doi.org/10.3390/ijms22115708

AMA Style

Mutoji KN, Sun M, Elliott G, Moreno IY, Hughes C, Gesteira TF, Coulson-Thomas VJ. Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice. International Journal of Molecular Sciences. 2021; 22(11):5708. https://doi.org/10.3390/ijms22115708

Chicago/Turabian Style

Mutoji, Kazadi N., Mingxia Sun, Garrett Elliott, Isabel Y. Moreno, Clare Hughes, Tarsis F. Gesteira, and Vivien J. Coulson-Thomas. 2021. "Extracellular Matrix Deposition and Remodeling after Corneal Alkali Burn in Mice" International Journal of Molecular Sciences 22, no. 11: 5708. https://doi.org/10.3390/ijms22115708

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop