Next Article in Journal
A MADS-Box Gene CiMADS43 Is Involved in Citrus Flowering and Leaf Development through Interaction with CiAGL9
Previous Article in Journal
Daily Lifestyle and Inflammatory Skin Diseases
Article

MAGEA4 Coated Extracellular Vesicles Are Stable and Can Be Assembled In Vitro

Institute of Technology, University of Tartu, 50411 Tartu, Estonia
*
Author to whom correspondence should be addressed.
Academic Editor: Leonora Balaj
Int. J. Mol. Sci. 2021, 22(10), 5208; https://doi.org/10.3390/ijms22105208
Received: 7 April 2021 / Revised: 2 May 2021 / Accepted: 11 May 2021 / Published: 14 May 2021
(This article belongs to the Section Biochemistry)
Extracellular vesicles (EVs) are valued candidates for the development of new tools for medical applications. Vesicles carrying melanoma-associated antigen A (MAGEA) proteins, a subfamily of cancer-testis antigens, are particularly promising tools in the fight against cancer. Here, we have studied the biophysical and chemical properties of MAGEA4-EVs and show that they are stable under common storage conditions such as keeping at +4 °C and −80 °C for at least 3 weeks after purification. The MAGEA4-EVs can be freeze-thawed two times without losing MAGEA4 in detectable quantities. The attachment of MAGEA4 to the surface of EVs cannot be disrupted by high salt concentrations or chelators, but the vesicles are sensitive to high pH. The MAGEA4 protein can bind to the surface of EVs in vitro, using robust passive incubation. In addition, EVs can be loaded with recombinant proteins fused to the MAGEA4 open reading frame within the cells and also in vitro. The high stability of MAGEA4-EVs ensures their potential for the development of EV-based anti-cancer applications. View Full-Text
Keywords: extracellular vesicles; cancer-testis antigen; MAGEA4; recombinant protein extracellular vesicles; cancer-testis antigen; MAGEA4; recombinant protein
Show Figures

Figure 1

MDPI and ACS Style

Reinsalu, O.; Samel, A.; Niemeister, E.; Kurg, R. MAGEA4 Coated Extracellular Vesicles Are Stable and Can Be Assembled In Vitro. Int. J. Mol. Sci. 2021, 22, 5208. https://doi.org/10.3390/ijms22105208

AMA Style

Reinsalu O, Samel A, Niemeister E, Kurg R. MAGEA4 Coated Extracellular Vesicles Are Stable and Can Be Assembled In Vitro. International Journal of Molecular Sciences. 2021; 22(10):5208. https://doi.org/10.3390/ijms22105208

Chicago/Turabian Style

Reinsalu, Olavi; Samel, Anneli; Niemeister, Elen; Kurg, Reet. 2021. "MAGEA4 Coated Extracellular Vesicles Are Stable and Can Be Assembled In Vitro" Int. J. Mol. Sci. 22, no. 10: 5208. https://doi.org/10.3390/ijms22105208

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop