Next Article in Journal
Added Value of Clinical Sequencing: WGS-Based Profiling of Pharmacogenes
Next Article in Special Issue
Residues in Transmembrane Segments of the P2X4 Receptor Contribute to Channel Function and Ethanol Sensitivity
Previous Article in Journal
Muscular Development in Urechis unicinctus (Echiura, Annelida)
Previous Article in Special Issue
Interaction between Calcium Chelators and the Activity of P2X7 Receptors in Mouse Motor Synapses
Open AccessArticle

Increased Purinergic Responses Dependent on P2Y2 Receptors in Hepatocytes from CCl4-Treated Fibrotic Mice

Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Querétaro 76230, Mexico
*
Author to whom correspondence should be addressed.
Current Address: Laboratorio Internacional de Investigación sobre el Genoma Humano, Universidad Nacional Autónoma de México, Querétaro 76230, Mexico.
Int. J. Mol. Sci. 2020, 21(7), 2305; https://doi.org/10.3390/ijms21072305
Received: 24 February 2020 / Accepted: 17 March 2020 / Published: 26 March 2020
(This article belongs to the Special Issue Purinergic P2 Receptors: Structure and Function)
Inflammatory and wound healing responses take place during liver damage, primarily in the parenchymal tissue. It is known that cellular injury elicits an activation of the purinergic signaling, mainly by the P2X7 receptor; however, the role of P2Y receptors in the onset of liver pathology such as fibrosis has not been explored. Hence, we used mice treated with the hepatotoxin CCl4 to implement a reversible model of liver fibrosis to evaluate the expression and function of the P2Y2 receptor (P2Y2R). Fibrotic livers showed an enhanced expression of P2Y2R that eliminated its zonal distribution. Hepatocytes from CCl4-treated mice showed an exacerbated ERK-phosphorylated response to the P2Y2R-specific agonist, UTP. Cell proliferation was also enhanced in the fibrotic livers. Hepatic transcriptional analysis by microarrays, upon CCl4 administration, showed that P2Y2 activation regulated diverse pathways, revealing complex action mechanisms. In conclusion, our data indicate that P2Y2R activation is involved in the onset of the fibrotic damage associated with the reversible phase of the hepatic damage promoted by CCl4. View Full-Text
Keywords: purinergic signaling; P2Y2 receptor; adenine nucleotides; hepatocyte; CCl4; liver fibrosis purinergic signaling; P2Y2 receptor; adenine nucleotides; hepatocyte; CCl4; liver fibrosis
Show Figures

Figure 1

MDPI and ACS Style

Velázquez-Miranda, E.; Molina-Aguilar, C.; González-Gallardo, A.; Vázquez-Martínez, O.; Díaz-Muñoz, M.; Vázquez-Cuevas, F.G. Increased Purinergic Responses Dependent on P2Y2 Receptors in Hepatocytes from CCl4-Treated Fibrotic Mice. Int. J. Mol. Sci. 2020, 21, 2305.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop