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Open AccessArticle

Waterpipe Tobacco Smoke Inhalation Triggers Thrombogenicity, Cardiac Inflammation and Oxidative Stress in Mice: Effects of Flavouring

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Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al Ain, UAE
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Zayed Center for Health Sciences, United Arab Emirates University, P.O. Box 17666 Al Ain, UAE
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Department of Pathology, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al Ain, UAE
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Department of Internal Medicine, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666 Al Ain, UAE
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Department of Pharmacology and Clinical Pharmacy, College of Medicine & Health Sciences, Sultan Qaboos University, P.O. Box 35, Muscat 123, Al-Khod, Oman
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1291; https://doi.org/10.3390/ijms21041291
Received: 16 December 2019 / Revised: 29 December 2019 / Accepted: 2 January 2020 / Published: 14 February 2020
(This article belongs to the Special Issue Inhalation Toxicology and Biological Response)
The consumption of water-pipe smoking (WPS) has been promoted by the use of flavoured tobacco. However, little is known about the impact of flavouring on the cardiovascular toxicity induced by WPS inhalation. Here, we compared the cardiovascular effects and underlying mechanism of actions of plain (P) (unflavoured) versus apple-flavoured (AF) WPS (30 minutes/day, 5 days/week for 1 month) in mice. Control mice were exposed to air. Both P- and AF-WPS inhalation induced an increase in systolic blood pressure, thrombogenicity and plasma concentration of fibrinogen and von Willebrand factor. In heart homogenates, AF-WPS inhalation caused an increase of 8-isoprostane and a decrease in the levels of reduced glutathione (GSH) and superoxide dismutase (SOD). Nevertheless, P-WPS decreased only the activity of SOD. The concentrations of tumour necrosis factor α and interleukin 1β were increased only in heart homogenates of mice exposed to AF-WPS. Although both P- and AF-WPS increased the concentration of troponin I in heart homogenates and induced DNA damage, the concentration of cleaved caspase 3 was only increased in mice exposed to AF-WPS. Immunohistochemical analysis of the hearts showed that both P- and AF- WPS inhalation decreased the expression of SOD. Moreover, the expression of nuclear factor erythroid-derived 2-like 2 at nuclear level in the heart was higher in both AF-WPS and P-WPS compared with control group, and the effect observed in AF-WPS group was more significant than that seen in P-WPS group. Likewise, the concentration of heme oxygenase-1 was significantly increased in both P-WPS and AF-WPS groups compared with control group, and the effect seen in AF-group was higher than that observed in P-WPS group. In conclusion, our findings showed that both P- and AF-WPS induce thrombogenicity and cardiac injury, and that this toxicity is potentiated by the presence of flavouring. View Full-Text
Keywords: waterpipe smoke; nose-only exposure; flavouring; heart; oxidative stress; inflammation; thrombosis waterpipe smoke; nose-only exposure; flavouring; heart; oxidative stress; inflammation; thrombosis
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Nemmar, A.; Al-Salam, S.; Beegam, S.; Yuvaraju, P.; Zaaba, N.E.; Yasin, J.; Ali, B.H. Waterpipe Tobacco Smoke Inhalation Triggers Thrombogenicity, Cardiac Inflammation and Oxidative Stress in Mice: Effects of Flavouring. Int. J. Mol. Sci. 2020, 21, 1291.

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