Next Article in Journal
Functionalized Polymeric Materials with Bio-Derived Antimicrobial Peptides for “Active” Packaging
Next Article in Special Issue
Proteomics Analysis of E. angustifolia Seedlings Inoculated with Arbuscular Mycorrhizal Fungi under Salt Stress
Previous Article in Journal
Region-Specific Neuroprotective Features of Astrocytes against Oxidative Stress Induced by 6-Hydroxydopamine
Previous Article in Special Issue
Physiological Analysis and Proteome Quantification of Alligator Weed Stems in Response to Potassium Deficiency Stress
Article

Identification of Early Salinity Stress-Responsive Proteins in Dunaliella salina by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis

1
Key Laboratory of Hydrobiology in Liaoning Province’s Universities, Dalian Ocean University, Dalian 116021, China
2
College of fisheries and life science, Dalian Ocean University, Dalian 116021, China
3
Bioinformatics Center, College of Life Sciences, Northwest A&F University, Yangling 712100, China
4
College of Life Sciences, Northwest University, Xi’an, Shaanxi 710069, China
*
Author to whom correspondence should be addressed.
These authors contribute equally to the work.
Int. J. Mol. Sci. 2019, 20(3), 599; https://doi.org/10.3390/ijms20030599
Received: 22 November 2018 / Revised: 31 December 2018 / Accepted: 16 January 2019 / Published: 30 January 2019
Salt stress is one of the most serious abiotic factors that inhibit plant growth. Dunaliella salina has been recognized as a model organism for stress response research due to its high capacity to tolerate extreme salt stress. A proteomic approach based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the proteome of D. salina during early response to salt stress and identify the differentially abundant proteins (DAPs). A total of 141 DAPs were identified in salt-treated samples, including 75 upregulated and 66 downregulated DAPs after 3 and 24 h of salt stress. DAPs were annotated and classified into gene ontology functional groups. The Kyoto Encyclopedia of Genes and Genomes pathway analysis linked DAPs to tricarboxylic acid cycle, photosynthesis and oxidative phosphorylation. Using search tool for the retrieval of interacting genes (STRING) software, regulatory protein–protein interaction (PPI) networks of the DAPs containing 33 and 52 nodes were built at each time point, which showed that photosynthesis and ATP synthesis were crucial for the modulation of early salinity-responsive pathways. The corresponding transcript levels of five DAPs were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). These results presented an overview of the systematic molecular response to salt stress. This study revealed a complex regulatory mechanism of early salt tolerance in D. salina and potentially contributes to developing strategies to improve stress resilience. View Full-Text
Keywords: Salinity stress; Dunaliella salina; isobaric tags for relative and absolute quantitation; differentially abundant proteins; proteomics Salinity stress; Dunaliella salina; isobaric tags for relative and absolute quantitation; differentially abundant proteins; proteomics
Show Figures

Figure 1

MDPI and ACS Style

Wang, Y.; Cong, Y.; Wang, Y.; Guo, Z.; Yue, J.; Xing, Z.; Gao, X.; Chai, X. Identification of Early Salinity Stress-Responsive Proteins in Dunaliella salina by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis. Int. J. Mol. Sci. 2019, 20, 599. https://doi.org/10.3390/ijms20030599

AMA Style

Wang Y, Cong Y, Wang Y, Guo Z, Yue J, Xing Z, Gao X, Chai X. Identification of Early Salinity Stress-Responsive Proteins in Dunaliella salina by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis. International Journal of Molecular Sciences. 2019; 20(3):599. https://doi.org/10.3390/ijms20030599

Chicago/Turabian Style

Wang, Yuan, Yuting Cong, Yonghua Wang, Zihu Guo, Jinrong Yue, Zhenyu Xing, Xiangnan Gao, and Xiaojie Chai. 2019. "Identification of Early Salinity Stress-Responsive Proteins in Dunaliella salina by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis" International Journal of Molecular Sciences 20, no. 3: 599. https://doi.org/10.3390/ijms20030599

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop