A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Handling and Ethics Statement
2.2. Experimental Design
2.3. Surgical Technique
2.4. Intraocular Pressure Measurements
2.5. Pattern Electroretinogram (PERG)
2.6. Full-Field Electroretinogram (ERG)
2.7. Spectral-Domain Optical Coherence Tomography (OCT)
2.8. Brn3a Immunohistochemistry
2.9. Histological Examination of the Anterior Segment
2.10. Data Analysis
3. Results
4. Discussion
Author Contributions
Funding
Conflicts of Interest
Abbreviations
OHT | Chronic ocular hypertension |
IOP | Intraocular pressure |
RGC | Retinal ganglion cells |
PERG | Pattern electroretinogram |
ERG | Full-field electroretinogram |
RNFL | Retinal nerve fiber layer |
OCT | Optical coherence tomography |
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Blanco, R.; Martinez-Navarrete, G.; Pérez-Rico, C.; Valiente-Soriano, F.J.; Avilés-Trigueros, M.; Vicente, J.; Fernandez, E.; Vidal-Sanz, M.; de la Villa, P. A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway. Int. J. Mol. Sci. 2019, 20, 3209. https://doi.org/10.3390/ijms20133209
Blanco R, Martinez-Navarrete G, Pérez-Rico C, Valiente-Soriano FJ, Avilés-Trigueros M, Vicente J, Fernandez E, Vidal-Sanz M, de la Villa P. A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway. International Journal of Molecular Sciences. 2019; 20(13):3209. https://doi.org/10.3390/ijms20133209
Chicago/Turabian StyleBlanco, Román, Gema Martinez-Navarrete, Consuelo Pérez-Rico, Francisco J. Valiente-Soriano, Marcelino Avilés-Trigueros, Javier Vicente, Eduardo Fernandez, Manuel Vidal-Sanz, and Pedro de la Villa. 2019. "A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway" International Journal of Molecular Sciences 20, no. 13: 3209. https://doi.org/10.3390/ijms20133209
APA StyleBlanco, R., Martinez-Navarrete, G., Pérez-Rico, C., Valiente-Soriano, F. J., Avilés-Trigueros, M., Vicente, J., Fernandez, E., Vidal-Sanz, M., & de la Villa, P. (2019). A Chronic Ocular-Hypertensive Rat Model induced by Injection of the Sclerosant Agent Polidocanol in the Aqueous Humor Outflow Pathway. International Journal of Molecular Sciences, 20(13), 3209. https://doi.org/10.3390/ijms20133209