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Int. J. Mol. Sci. 2018, 19(10), 3133; https://doi.org/10.3390/ijms19103133

Transcriptome Profiling Reveals Transcriptional Regulation by DNA Methyltransferase Inhibitor 5-Aza-2′-Deoxycytidine Enhancing Red Pigmentation in Bagged “Granny Smith” Apples (Malus domestica)

1
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, China
2
Shaanxi Research Center of Apple Engineering and Technology, Yangling 712100, China
*
Author to whom correspondence should be addressed.
Received: 10 August 2018 / Revised: 2 October 2018 / Accepted: 9 October 2018 / Published: 12 October 2018
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

The red color of apples (Malus domestica) is an attractive trait for consumers. The green skinned “Granny Smith” cultivar develops red pigmentation after bagging treatment. DNA methylation plays an important role in various developmental processes in plants. To explore the possible functions of DNA methylation in the pigmentation of bagged “Granny Smith” apples, we first analyzed the anthocyanin content of fruit skin following treatment with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine (5-aza-dC). The results revealed an increase in anthocyanin content in bagged fruits following 5-aza-dC treatment, while no anthocyanins were detected in unbagged fruits. In addition, 8482 differentially expressed genes between 5-aza-dC-treated and control groups were identified in bagged fruits by RNA sequencing, including genes encoding transcription factors, enzymes related to anthocyanin accumulation, and methylases. Changes in the expression of these genes may be responsible for 5-aza-dC-induced red pigmentation in bagged fruits of “Granny Smith”. The findings provide novel evidence for the involvement of DNA methylation in the red pigmentation of non-red-skinned apples. View Full-Text
Keywords: Granny Smith; anthocyanin; transcriptome; 5-aza-dC; MYB; DNA methylation Granny Smith; anthocyanin; transcriptome; 5-aza-dC; MYB; DNA methylation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Ma, C.; Liang, B.; Chang, B.; Liu, L.; Yan, J.; Yang, Y.; Zhao, Z. Transcriptome Profiling Reveals Transcriptional Regulation by DNA Methyltransferase Inhibitor 5-Aza-2′-Deoxycytidine Enhancing Red Pigmentation in Bagged “Granny Smith” Apples (Malus domestica). Int. J. Mol. Sci. 2018, 19, 3133.

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