Next Article in Journal
Somatic Genetic Variation in Solid Pseudopapillary Tumor of the Pancreas by Whole Exome Sequencing
Previous Article in Journal
Microalgal Cultivation in Secondary Effluent: Recent Developments and Future Work
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(1), 60;

A Comprehensive Proteomic Survey of ABA-Induced Protein Phosphorylation in Rice (Oryza sativa L.)

State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China
Agricultural Genomes Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
College of Agricultural Sciences, Hunan Agricultural University, Changsha 410128, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Setsuko Komatsu
Received: 7 November 2016 / Revised: 13 December 2016 / Accepted: 22 December 2016 / Published: 3 January 2017
(This article belongs to the Section Biochemistry)
Full-Text   |   PDF [2309 KB, uploaded 3 January 2017]   |  


abscisic acid (ABA) is a key phytohormone regulating plant development and stress response. The signal transduction of ABA largely relies on protein phosphorylation. However; little is known about the phosphorylation events occurring during ABA signaling in rice thus far. By employing a label-free; MS (Mass Spectrometry)-based phosphoproteomic approach; we identified 2271 phosphosites of young rice seedlings and their intensity dynamics in response to ABA; during which 1060 proteins were found to be differentially phosphorylated. Western-blot analysis verified the differential phosphorylation pattern of D1, SMG1 and SAPK9 as indicated by the MS result; suggesting the high reliability of our phosphoproteomic data. The DP (differentially phosphorylated) proteins are extensively involved in ABA as well as other hormone signaling pathways. It is suggested that ABA antagonistically regulates brassinosteroid (BR) signaling via inhibiting BR receptor activity. The result of this study not only expanded our knowledge of rice phosphoproteome, but also shed more light on the pattern of protein phosphorylation in ABA signaling. View Full-Text
Keywords: rice (Oryza sativa L.); abscisic acid; signaling; phosphoproteome rice (Oryza sativa L.); abscisic acid; signaling; phosphoproteome

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Qiu, J.; Hou, Y.; Wang, Y.; Li, Z.; Zhao, J.; Tong, X.; Lin, H.; Wei, X.; Ao, H.; Zhang, J. A Comprehensive Proteomic Survey of ABA-Induced Protein Phosphorylation in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2017, 18, 60.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top