Next Article in Journal
Locus Number Estimation of MHC Class II B in Stone Flounder and Japanese Flounder
Next Article in Special Issue
Unfolding the Unfolded Protein Response: Unique Insights into Brain Ischemia
Previous Article in Journal
RNA-Seq Transcriptome Analysis of Maize Inbred Carrying Nicosulfuron-Tolerant and Nicosulfuron-Susceptible Alleles
Previous Article in Special Issue
Methemoglobin Is an Endogenous Toll-Like Receptor 4 Ligand—Relevance to Subarachnoid Hemorrhage
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessLetter
Int. J. Mol. Sci. 2015, 16(3), 5990-5999;

Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
College of Wildlife Resources, Northeast Forestry University, Harbin 150040, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Xiaofeng Jia
Received: 22 December 2014 / Revised: 4 February 2015 / Accepted: 10 February 2015 / Published: 13 March 2015
(This article belongs to the Special Issue Neurological Injuries’ Monitoring, Tracking and Treatment)
Full-Text   |   PDF [1480 KB, uploaded 13 March 2015]   |  


We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes). View Full-Text
Keywords: bovine; neural stem cell; differentiation bovine; neural stem cell; differentiation

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Gao, Y.; Li, X.; Zheng, D.; Guan, W.; Ma, Y. Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain. Int. J. Mol. Sci. 2015, 16, 5990-5999.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top