Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation, Culture, and Morphology of NSCs
2.2. Optimization of NSCs
2.3. Characterization of NSCs
2.3.1. Markers of NSCs
2.3.2. Neurogenic Differentiation of NSCs
2.4. Discussion
3. Experimental Section
3.1. Reagents and Animals
3.2. Isolation of NSCs from Bovine Brain Embryo
3.3. Optimization of Cell Culture Systems for NSCs
3.4. Marker of NSCs
3.5. RT-PCR Assays
Gene | Primer Sequence | Tm (°C) | Fragment Size (bp) |
---|---|---|---|
Sox 2 | F: 5'-TCCTATTCTCAGCAGGGCAC-3' | 61 | 217 |
R: 5'-AGTGCTGGGACATGTGAAGT-3' | |||
GAPDH | F: 5'-GGCAAGTTCAACGGCACAGTCA-3' | 58 | 364 |
R: 5'-TAAGTCCCTCCACGATGCCAAAG-3' | |||
GFAP | F: 5'-GTCGTGGGTGAGCAGTTACA-3' | 58 | 341 |
R: 5'-CTAAAACACGGGGAGGTGGG-3' | |||
MBP | F: 5'-CAGAGACACTGGCATCCTCG-3' | 60 | 350 |
R: 5'-CAGACGCTCTGCCTCCATAG-3' | |||
MAP2 | F: 5'-CGCCAATGGATTCCCCTACA-3' | 54 | 322 |
R: 5'-TTCCTCCACTGGGACAGTCT-3' | |||
Musashi | F: 5'-GTCTCGAGTCATGCCCTACG-3' | 55 | 223 |
F: 5'-CATGGGTCCATAAGCGGTGA-3' |
3.6. Differentiation
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Gao, Y.; Li, X.; Zheng, D.; Guan, W.; Ma, Y. Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain. Int. J. Mol. Sci. 2015, 16, 5990-5999. https://doi.org/10.3390/ijms16035990
Gao Y, Li X, Zheng D, Guan W, Ma Y. Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain. International Journal of Molecular Sciences. 2015; 16(3):5990-5999. https://doi.org/10.3390/ijms16035990
Chicago/Turabian StyleGao, Yuhua, Xiangchen Li, Dong Zheng, Weijun Guan, and Yuehui Ma. 2015. "Isolation of a Pluripotent Neural Stem Cell from the Embryonic Bovine Brain" International Journal of Molecular Sciences 16, no. 3: 5990-5999. https://doi.org/10.3390/ijms16035990