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Open AccessArticle

Protein Profiling of Blood Samples from Patients with Hereditary Leiomyomatosis and Renal Cell Cancer by Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

1
Department of Urology, Dokkyo Medical University, Tochigi 321-0293, Japan
2
Proteomics Research Unit, Division of Advanced Medicine, Medical Research Institute, Kanazawa Medical University, Ishikawa 920-0293, Japan
3
Department of Radiology, Dokkyo Medical University, Tochigi 321-0293, Japan
4
Department of Pathology, Dokkyo Medical University, Tochigi 321-0293, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(11), 14518-14532; https://doi.org/10.3390/ijms131114518
Received: 31 July 2012 / Revised: 1 November 2012 / Accepted: 5 November 2012 / Published: 8 November 2012
(This article belongs to the Special Issue Advances in Molecular Oncology (special issue))
Hereditary leiomyomatosis and renal cell cancer (HLRCC) is an extremely rare syndrome with autosomal dominant inheritance. HLRCC is characterized by a predisposition to leiomyomas of the skin and the uterus as well as renal cell carcinoma. The disease-related gene has been identified as fumarate hydratase (fumarase, FH), which encodes an enzyme involved in the mitochondrial tricarboxylic acid cycle. Protein profiling may give some insight into the molecular pathways of HLRCC. Therefore, we performed protein profiling of blood samples from HLRCC patients, their family members, and healthy volunteers, using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) coupled with IMAC-Cu chips. For hierarchical clustering analysis, we used the 45 peaks that revealed significant differences in single-marker analysis over the range from 1500 to 15,000 m/z. Heat map analysis based on the results of clustering distinguished the HLRCC kindred from non-HLRCC subjects with a sensitivity of 94% and a specificity of 90%. SELDI-TOF MS profiling of blood samples can be applied to identify patients with HLRCC and to assess specific molecular mechanisms involved in this condition. View Full-Text
Keywords: Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC); fumarate hydratase (FH); surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS); metastasis Hereditary Leiomyomatosis and Renal Cell Cancer (HLRCC); fumarate hydratase (FH); surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS); metastasis
MDPI and ACS Style

Kamai, T.; Tomosugi, N.; Abe, H.; Kaji, Y.; Oyama, T.; Yoshida, K.-I. Protein Profiling of Blood Samples from Patients with Hereditary Leiomyomatosis and Renal Cell Cancer by Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Int. J. Mol. Sci. 2012, 13, 14518-14532.

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