Next Article in Journal
Research on Improving Pretreatment Process of Banana Fiber Fabric with Rare Earth
Previous Article in Journal
Chemical Profiling of Latvian Propolis: Regional Variations and Botanical Origins
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 30
Issue 23
10.3390/molecules30234531
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Extraction and Identification of Active Components from Lilium lancifolium Based on NADES-UHPLC-MS/MS Technology

by
Yuliang Wang
1,2,
Yingjie Ma
2,
Zhenxu Jiang
2,
Weiwei Tang
2,
Chaoxing Wang
2,*,
Hong Zhao
2,* and
Yu Zhang
1,2,*
1
Key Laboratory of Microecology-Immune Regulatory Network and Related Diseases, School of Basic Medicine, Jiamusi University, Jiamusi 154007, China
2
Heilongjiang Provincial Key Laboratory of New Drug Development and Pharmacotoxicological Evaluation, College of Pharmacy, Jiamusi University, Jiamusi 154007, China
*
Authors to whom correspondence should be addressed.
Molecules 2025, 30(23), 4531; https://doi.org/10.3390/molecules30234531
Submission received: 27 September 2025 / Revised: 21 November 2025 / Accepted: 22 November 2025 / Published: 24 November 2025
(This article belongs to the Topic Advances on the Application of Nanomaterials and 2D Materials for Sensors, Solar Cells, Microelectronics, and Optoelectronics)
Browse Figures
Versions Notes

Abstract

The bulb of Lilium lancifolium, a traditional Chinese medicine and food-homologous material, is rich in various saponins with notable pharmacological activities. However, traditional extraction methods using single solvents suffer from low efficiency, high cost, and flammability. To address these limitations, this study developed a green and efficient extraction method using natural deep eutectic solvents (NADES). Twenty-four NADES were synthesized and screened for stability, leading to the selection of fourteen for subsequent extraction of saponins from L. lancifolium, with ethanol extraction as a control. Through optimization, NADES-15, composed of choline chloride and anhydrous citric acid (2:1), was identified as the most effective solvent. Quantitative analysis revealed that the total saponin content obtained with NADES-15 (46.6 mg/g) significantly surpassed that of ethanol. Furthermore, UHPLC-MS/MS analysis demonstrated a greater diversity of chemical components in the NADES-15 extract, wherein 31 compounds were tentatively identified, including all nine target steroidal saponins (lily saponin A–I), compared to only 17 compounds and six saponins found in the ethanol extract. This study highlights the significant advantages of NADES in enhancing both the efficiency and variety of active component extraction from L. lancifolium, offering a novel and green approach for natural product extraction.
Keywords: Lilium lancifolium; saponin; natural deep eutectic solvent; plant active components; UHPLC-MS/MS Lilium lancifolium; saponin; natural deep eutectic solvent; plant active components; UHPLC-MS/MS
Graphical Abstract

Share and Cite

MDPI and ACS Style

Wang, Y.; Ma, Y.; Jiang, Z.; Tang, W.; Wang, C.; Zhao, H.; Zhang, Y. Extraction and Identification of Active Components from Lilium lancifolium Based on NADES-UHPLC-MS/MS Technology. Molecules 2025, 30, 4531. https://doi.org/10.3390/molecules30234531

AMA Style

Wang Y, Ma Y, Jiang Z, Tang W, Wang C, Zhao H, Zhang Y. Extraction and Identification of Active Components from Lilium lancifolium Based on NADES-UHPLC-MS/MS Technology. Molecules. 2025; 30(23):4531. https://doi.org/10.3390/molecules30234531

Chicago/Turabian Style

Wang, Yuliang, Yingjie Ma, Zhenxu Jiang, Weiwei Tang, Chaoxing Wang, Hong Zhao, and Yu Zhang. 2025. "Extraction and Identification of Active Components from Lilium lancifolium Based on NADES-UHPLC-MS/MS Technology" Molecules 30, no. 23: 4531. https://doi.org/10.3390/molecules30234531

APA Style

Wang, Y., Ma, Y., Jiang, Z., Tang, W., Wang, C., Zhao, H., & Zhang, Y. (2025). Extraction and Identification of Active Components from Lilium lancifolium Based on NADES-UHPLC-MS/MS Technology. Molecules, 30(23), 4531. https://doi.org/10.3390/molecules30234531

Article Metrics

Back to TopTop