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Article

Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease

1
Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba 80240-020, Brazil
2
Centre de Recherches sur les Macromolécules Végétales (CERMAV), Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes, F-38000 Grenoble, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Wansong Chen and Jianhua Zhang
Molecules 2022, 27(9), 2811; https://doi.org/10.3390/molecules27092811
Received: 13 March 2022 / Revised: 16 April 2022 / Accepted: 23 April 2022 / Published: 28 April 2022
(This article belongs to the Special Issue Functional Nanomaterials in Biomedicine)
Background: Parkinson’s disease (PD) is the second most common age-related neurodegenerative disorder. Levodopa (L-DOPA) remains the gold-standard drug available for treating PD. Curcumin has many pharmacological activities, including antioxidant, anti-inflammatory, antimicrobial, anti-amyloid, and antitumor properties. Copolymers composed of Poly (ethylene oxide) (PEO) and biodegradable polyesters such as Poly (ε-caprolactone) (PCL) can self-assemble into nanoparticles (NPs). This study describes the development of NH2–PEO–PCL diblock copolymer positively charged and modified by adding glutathione (GSH) on the outer surface, resulting in a synergistic delivery of L-DOPA curcumin that would be able to pass the blood–brain barrier. Methods: The NH2–PEO–PCL NPs suspensions were prepared by using a nanoprecipitation and solvent displacement method and coated with GSH. NPs were submitted to characterization assays. In order to ensure the bioavailability, Vero and PC12 cells were treated with various concentrations of the loaded and unloaded NPs to observe cytotoxicity. Results: NPs have successfully loaded L-DOPA and curcumin and were stable after freeze-drying, indicating advancing into in vitro toxicity testing. Vero and PC12 cells that were treated up to 72 h with various concentrations of L-DOPA and curcumin-loaded NP maintained high viability percentage, indicating that the NPs are biocompatible. Conclusions: NPs consisting of NH2–PEO–PCL were characterized as potential formulations for brain delivery of L-DOPA and curcumin. The results also indicate that the developed biodegradable nanomicelles that were blood compatible presented low cytotoxicity. View Full-Text
Keywords: nanoparticles; glutathione; Parkinson’s disease; L-DOPA; curcumin nanoparticles; glutathione; Parkinson’s disease; L-DOPA; curcumin
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MDPI and ACS Style

Mogharbel, B.F.; Cardoso, M.A.; Irioda, A.C.; Stricker, P.E.F.; Slompo, R.C.; Appel, J.M.; de Oliveira, N.B.; Perussolo, M.C.; Saçaki, C.S.; da Rosa, N.N.; Dziedzic, D.S.M.; Travelet, C.; Halila, S.; Borsali, R.; de Carvalho, K.A.T. Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease. Molecules 2022, 27, 2811. https://doi.org/10.3390/molecules27092811

AMA Style

Mogharbel BF, Cardoso MA, Irioda AC, Stricker PEF, Slompo RC, Appel JM, de Oliveira NB, Perussolo MC, Saçaki CS, da Rosa NN, Dziedzic DSM, Travelet C, Halila S, Borsali R, de Carvalho KAT. Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease. Molecules. 2022; 27(9):2811. https://doi.org/10.3390/molecules27092811

Chicago/Turabian Style

Mogharbel, Bassam F., Marco A. Cardoso, Ana C. Irioda, Priscila E.F. Stricker, Robson C. Slompo, Julia M. Appel, Nathalia B. de Oliveira, Maiara C. Perussolo, Claudia S. Saçaki, Nadia N. da Rosa, Dilcele S.M. Dziedzic, Christophe Travelet, Sami Halila, Redouane Borsali, and Katherine A.T. de Carvalho. 2022. "Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease" Molecules 27, no. 9: 2811. https://doi.org/10.3390/molecules27092811

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