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Article

Very Stable Two Mega Dalton High-Molecular-Mass Multiprotein Complex from Sea Cucumber Eupentacta fraudatrix

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Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences, Lavrentiev Ave. 8, 630090 Novosibirsk, Russia
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G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Brunch of the Russian Academy of Sciences, 159 Pr. 100 Let Vladivostoku, 690022 Vladivostok, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Fabio Ganazzoli
Molecules 2021, 26(18), 5703; https://doi.org/10.3390/molecules26185703
Received: 13 July 2021 / Revised: 16 August 2021 / Accepted: 25 August 2021 / Published: 21 September 2021
In contrast to many human organs, only the human liver can self-regenerate, to some degree. Some marine echinoderms are convenient objects for studying the processes of regenerations of organs and tissues. For example, sea cucumbers Eupentacta fraudatrix can completely restore within several weeks, the internal organs and the whole body after their division into two or three parts. Therefore, these cucumbers are a very convenient model for studying the general mechanisms of regeneration. However, there is no literature data yet on which biomolecules of these cucumbers can stimulate the regeneration of organs and the whole-body processes. Studying the mechanisms of restoration is very important for modern biology and medicine, since it can help researchers to understand which proteins, enzymes, hormones, or possible complexes can play an essential role in regeneration. This work is the first to analyze the possible content of very stable protein complexes in sea cucumbers Eupentacta fraudatrix. It has been shown that their organisms contain a very stable multiprotein complex of about 2000 kDa. This complex contains 15 proteins with molecular masses (MMs) >10 kDa and 21 small proteins and peptides with MMs 2.0–8.6 kDa. It is effectively destroyed only in the presence of 3.0 M MgCl2 and, to a lesser extent, 3.0 M NaCl, while the best dissociation occurs in the presence of 8.0 M urea + 0.1 M EDTA. Our data indicate that forming a very stable proteins complex occurs due to the combination of bridges formed by metal ions, electrostatic contacts, and hydrogen bonds. View Full-Text
Keywords: model of tissues and body regeneration; sea cucumber Eupentacta fraudatrix; very stable 2000 kDa protein complex model of tissues and body regeneration; sea cucumber Eupentacta fraudatrix; very stable 2000 kDa protein complex
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MDPI and ACS Style

Timofeeva, A.M.; Kostrikina, I.A.; Dmitrenok, P.S.; Soboleva, S.E.; Nevinsky, G.A. Very Stable Two Mega Dalton High-Molecular-Mass Multiprotein Complex from Sea Cucumber Eupentacta fraudatrix. Molecules 2021, 26, 5703. https://doi.org/10.3390/molecules26185703

AMA Style

Timofeeva AM, Kostrikina IA, Dmitrenok PS, Soboleva SE, Nevinsky GA. Very Stable Two Mega Dalton High-Molecular-Mass Multiprotein Complex from Sea Cucumber Eupentacta fraudatrix. Molecules. 2021; 26(18):5703. https://doi.org/10.3390/molecules26185703

Chicago/Turabian Style

Timofeeva, Anna M., Irina A. Kostrikina, Pavel S. Dmitrenok, Svetlana E. Soboleva, and Georgy A. Nevinsky. 2021. "Very Stable Two Mega Dalton High-Molecular-Mass Multiprotein Complex from Sea Cucumber Eupentacta fraudatrix" Molecules 26, no. 18: 5703. https://doi.org/10.3390/molecules26185703

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