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Open AccessArticle

Extraction of Phenolic Compounds from Tabernaemontana catharinensis Leaves and Their Effect on Oxidative Stress Markers in Diabetic Rats

Department of Chemistry, Federal University of Technology—Paraná (UTFPR), P.O. Box 591, 85503-390 Pato Branco, Brazil
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Molecules 2020, 25(10), 2391; https://doi.org/10.3390/molecules25102391
Received: 1 April 2020 / Revised: 19 April 2020 / Accepted: 29 April 2020 / Published: 21 May 2020
The aim of this study was to evaluate the most effective extraction condition (temperature, solvent type and time) for recovery of high-value phytochemicals present in the Tabernaemontana catharinensis leaves (TC) and to assess their effect on biochemical parameters in streptozotocin-induced diabetic rats. The extraction of phenolic compounds from TC using a factorial design (FD) 2³, high performance liquid chromatography (HPLC), response surface methodology (RSM) and principal component analysis (PCA) were studied. It was found that the optimal conditions for extraction of phenolics were higher temperature (65 °C) and time (60 min) using ethanol as extractor solvent. In this condition of extraction (A8), total phenolic compounds (TPC) and antioxidant activity (AA) were determined. Additionally, this extract was used to evaluate their effect on antioxidant enzyme activities (superoxide dismutase (SOD) and catalase (CAT)) as well as lipid peroxidation (LP) and protein thiols level (PSH) in the liver and kidneys of normal and diabetic rats. As result, T. catharinensis extract presented TPC content of 23.34 mg EAG/g (equivalent gallic acid) and AA of 34.26 μmol Trolox/g. Phenolic acids (ferulic acid and coumaric acid) and flavonoids (quercetin, rutin and pinocembrin) could be recovered and identified by HPLC. This study indicated an important role of the T. catharinensis extract on free radical inactivation and on the antioxidant defense system in diabetic rats. In fact, the use of T. catharinensis extract restored the normal activity of SOD (p < 0.05) and suppressed malondialdehyde levels in liver and kidney tissues. Thus, the T. catharinensis extract, rich in phenolic compounds, can be responsible for the recover the enzymatic changes in the liver and kidney tissues provoked by diabetes in rats. In addition, the lipid peroxidation rate decreased in the diabetic rats treated with T. catharinensis. View Full-Text
Keywords: phenolic compound; high performance liquid chromatography; diabetes; superoxide dismutase; catalase activity; Tabernaemontana catharinensis phenolic compound; high performance liquid chromatography; diabetes; superoxide dismutase; catalase activity; Tabernaemontana catharinensis
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MDPI and ACS Style

Sari, R.; Conterno, P.; da Silva, L.D.; de Lima, V.A.; Oldoni, T.L.C.; Thomé, G.R.; Carpes, S.T. Extraction of Phenolic Compounds from Tabernaemontana catharinensis Leaves and Their Effect on Oxidative Stress Markers in Diabetic Rats. Molecules 2020, 25, 2391.

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