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Open AccessArticle

Evaluation of Antioxidant and Antibacterial Activities, Cytotoxicity of Acacia seyal Del Bark Extracts and Isolated Compounds

1
Université de Lorraine, CNRS, L2CM, F-54000 Nancy, France
2
Medicinal Research Institute, Centre d’Etudes et de Recherche de Djibouti, IRM-CERD, Route de l’Aéroport, Haramous B.P. 486, Djibouti
3
ABC Platform®, Faculté de Pharmacie, F-54505 Vandoeuvre-lès-Nancy, France
*
Author to whom correspondence should be addressed.
Academic Editors: Halina Ekiert and Agnieszka Szopa
Molecules 2020, 25(10), 2392; https://doi.org/10.3390/molecules25102392
Received: 27 April 2020 / Revised: 11 May 2020 / Accepted: 13 May 2020 / Published: 21 May 2020
(This article belongs to the Special Issue Biological Activities of Natural Products)
Water extract of Acacia seyal bark is used traditionally by the population in Djibouti for its anti-infectious activity. The evaluation of in vitro antibacterial, antioxidant activities and cytotoxicity as well as chemical characterization of Acacia seyal bark water and methanolic extracts were presented. The water extract has a toxicity against the MRC-5 cells at 256 μg/mL while the methanolic extract has a weak toxicity at the same concentration. The methanolic extract has a strong antioxidant activity with half maximal inhibitory concentration (IC50) of 150 ± 2.2 μg/mL using 1-diphenyl-2-picrylhydrazyl (DPPH) and IC50 of 27 ± 1.3 μg/mL using 2,2′-azino-bis 3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical methods. For ferric reducing/antioxidant power (FRAP) assay, the result is 45.74 ± 5.96 μg Vitamin C Equivalent (VCE)/g of dry weight (DW). The precipitation of tannins from methanol crude extract decreases the MIC from 64 µg/mL to 32 µg/mL against Staphylococcus aureus and Corynebacterium urealyticum. However, the antioxidant activity is higher before tannins precipitation than after (IC50 = 150 µg/mL for methanolic crude extract and 250 µg/mL after tannins precipitation determined by DPPH method). By matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, the results showed that the condensed tannins consist of two types of catechin and gallocatechin-based oligomers. The fractionation led to the identification of three pure compounds: two flavanols catechin and epicatechin; one triterpene as lupeol; and a mixture of three steroids and one fatty acid: campesterol, stigmasterol, clionasterol, and oleamide. View Full-Text
Keywords: Acacia seyal bark; in vitro antibacterial properties; polyphenols; antioxidant activity; tannins; MALDI-TOF Acacia seyal bark; in vitro antibacterial properties; polyphenols; antioxidant activity; tannins; MALDI-TOF
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Elmi, A.; Spina, R.; Risler, A.; Philippot, S.; Mérito, A.; Duval, R.E.; Abdoul-latif, F.M.; Laurain-Mattar, D. Evaluation of Antioxidant and Antibacterial Activities, Cytotoxicity of Acacia seyal Del Bark Extracts and Isolated Compounds. Molecules 2020, 25, 2392.

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