Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from Caribena versicolor Spider Molt Cuticle
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Materials
3.2. Chitin Extraction
3.2.1. Microwave-Assisted Chitin Isolation
3.2.2. Estimation of Chitin Content in Molt
3.2.3. Pigment Isolation
3.3. Characterization of Obtained Materials
3.3.1. Light, Fluorescence, and Stereo Microscopy
3.3.2. Calcofluor White Staining
3.3.3. Scanning Electron Microscopy (SEM)
3.3.4. Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy
3.3.5. Raman Spectroscopy
3.3.6. X-ray Diffraction (XRD)
3.3.7. Electrospray Ionization Mass Spectrometry (ESI-MS)
3.3.8. 13C Solid State NMR
3.3.9. Chitinase Digestion Test
3.3.10. UV-VIS Spectroscopy
3.3.11. Parameters of the Porous Structure
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Machałowski, T.; Wysokowski, M.; Tsurkan, M.V.; Galli, R.; Schimpf, C.; Rafaja, D.; Brendler, E.; Viehweger, C.; Żółtowska-Aksamitowska, S.; Petrenko, I.; et al. Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from Caribena versicolor Spider Molt Cuticle. Molecules 2019, 24, 3736. https://doi.org/10.3390/molecules24203736
Machałowski T, Wysokowski M, Tsurkan MV, Galli R, Schimpf C, Rafaja D, Brendler E, Viehweger C, Żółtowska-Aksamitowska S, Petrenko I, et al. Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from Caribena versicolor Spider Molt Cuticle. Molecules. 2019; 24(20):3736. https://doi.org/10.3390/molecules24203736
Chicago/Turabian StyleMachałowski, Tomasz, Marcin Wysokowski, Mikhail V. Tsurkan, Roberta Galli, Christian Schimpf, David Rafaja, Erica Brendler, Christine Viehweger, Sonia Żółtowska-Aksamitowska, Iaroslav Petrenko, and et al. 2019. "Spider Chitin: An Ultrafast Microwave-Assisted Method for Chitin Isolation from Caribena versicolor Spider Molt Cuticle" Molecules 24, no. 20: 3736. https://doi.org/10.3390/molecules24203736