Search Results (67)

Tyromyces chioneus fruiting bodies could potentially be used for food and medicine; however, we still have limited knowledge about their optimal cultivation conditions and nutritional quality. In this study, we cultivated a wild strain of T. chioneus collected from Sandaoguan National Forest Park in Mudanjiang City. We compared the growth and yield of fruiting bodies on a sawdust substrate alone or in combination with corncobs, soybean straw, or corn straw. We also determined the contents of crude protein, crude polysaccharide, dietary fiber, total flavonoids, and other nutrients in the fruiting bodies and the ability of T. chioneus to degrade cellulose, hemicellulose, and lignin when grown on a sawdust–corncob substrate. T. chioneus produced two flushes of fruiting bodies on all tested substrates, but the mycelial colonization time, primordial initiation time, and time interval between flushes varied among the substrates, with ranges of 28.00 ± 1.00–30.67 ± 0.58 d, 9.33 ± 0.58–11.67 ± 0.58 d, and 11.33 ± 0.5–13.00 ± 1.00 d, respectively. The sawdust–corncob substrate resulted in a substantially higher biological efficiency (BE) of 35.14 ± 0.93% compared to the previously reported 19.15%, along with the highest yield of 196.80 ± 5.21 g bag⁻¹. The contents of total flavonoids, crude protein, dietary fiber, and calcium of the fruiting bodies produced on the sawdust–corncob substrate were 708 mg 100 g⁻¹, 16.30 g 100 g⁻¹, 18.82 g 100 g⁻¹, and 554 mg kg⁻¹, respectively. The degradation rates of lignin and cellulose in the sawdust–corncob substrate by T. chioneus were 33.93% and 37.72%, respectively. This study suggests that sawdust–corncob could be a promising substrate to cultivate T. chioneus. Full article

This study investigated, for the first time, the phenolic acid profile along with the in vitro antioxidant and acetylcholinesterase (AChE) inhibitory activities of leaves and fruits from five Romanian wild-grown Acer spp. (Sapindaceae): A. campestre L., A. monspessulanum L., A. platanoides L., A. pseudoplatanus L., and A. tataricum L. High-performance liquid chromatography coupled with ultraviolet and mass spectrometry (HPLC/UV/MS) was employed to identify and quantify key phenolic acids, including gallic, caffeic, protocatechuic, chlorogenic, and p-coumaric acids. Total phenolic content (TPC) ranged from 61.48 ± 3.76 to 512.8 ± 20.54 µg gallic acid equivalents (GAE)/mL. Antioxidant activities, assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, demonstrated the strongest radical scavenging capacities for A. tataricum fruits, with half maximal inhibitory concentration (IC₅₀) values of 10.88 ± 3.39 µg/mL and 10.39 ± 2.86 µg/mL, respectively. The ferric-reducing antioxidant power (FRAP) assay revealed the highest reducing power for A. tataricum fruits (1158 ± 48.98 µmol Fe²⁺/L) and leaves (1119 ± 164.6 µmol Fe²⁺/L). AChE inhibition was only found in A. tataricum, with an IC₅₀ of 7.91 ± 7.50 mg/mL for leaves and 15.95 ± 8.52 mg/mL for fruits. These results highlight A. tataricum as a promising source of natural antioxidants and neuroprotective agents. Full article

This study focused on determining the content of bioactive compounds in selected fruits of wild shrubs. The plants selected for the study were from the Rosaceae and Adoxaceae families. Particular attention should be paid to the fruits of plants commonly growing in Poland (temperate climate), such as Crataegus monogyna, Sorbus aucuparia, Viburnum opulus, and Sambucus nigra. The study aimed to deepen the knowledge of the content of selected secondary metabolites, such as phenolic acids, flavonoids, flavonoid glycosides, and their antioxidant properties, as well as natural dyes. During this study, chromatographic and spectrophotometric methods were used to determine the quantitative profile of the above-mentioned secondary metabolites of wild plant fruits. The quantitative profile of 16 phenolic acids, 9 flavonoids, 5 organic acids, 13 flavonoid glycosides, and 3 natural dyes was determined. Based on the studies, it was noted that the qualitative and quantitative profile of the bioactive compounds differs not only depending on the species but also on the location where the plant grows. A statistical analysis showed significant differences (p < 0.05) in the content of phenols and flavonoids in fruits collected from different locations. Interestingly, differences were also observed within the species, probably depending on the geographical location and composition of the soil in which the plants were grown. Full article

The evaluation of quality traits is an important procedure for kiwifruit breeding and comprehensive utilization. The present study aimed to establish a comprehensive system to assess Actinidia eriantha germplasms by analyzing 22 quality traits on kiwifruit samples collected from a wild population of 236 plants grown in the Jiangxi Province, China. Variability, correlation, principal components, and cluster analyses were carried out using the data collected from fruit quality evaluations. The coefficients of variation (CV) of fruit quality traits ranged from 11.66 to 66.16% (average coefficient = 35.09%), indicating a high level of variation among the 236 plants. In addition, different degrees of correlations were found between the traits, with similar traits demonstrating strong correlations. Principal component analysis (PCA) generated eight comprehensive and independent principal components, accounting for 77.93% of the original fruit quality information. Furthermore, an extensive evaluation from PCA ranked the plants based on cluster analysis and grouped them into seven categories. A stepwise regression analysis generated a prediction model, demonstrating a good fit (0.945) with the principal components of the comprehensive evaluation score. Overall, this study identifies nine quality traits, representing fruit appearance, sweetness, acidity, flavor, and nutritional attributes, as important traits for a comprehensive evaluation of A. eriantha fruits. Full article

The aim of the research was to determine the yield, chemical composition and free radical-scavenging activity of the essential oils (EOs) in the leaves and fruit of wild-grown bay (Laurus nobilis L.) and rosemary(Rosmarinus officinalis L.)from the Montenegro coast. The bay essential oil (BEO) yield was 0.88% in fruit and 2.65% in the leaves. The rosemary essential oil (REO) yield was 1.30%. BEOs obtained from the leaves and fruit display different compositions. Fifty components were identified in leaf BEO, with 1,8-cineole (39.4%), linalool (13.9%), α-terpinyl acetate (11.2%), sabinene (6.7%) and methyl eugenol (5.7%) being the most abundant ones. Fifty-five components were isolated from fruit BEO, with 1,8-cineole (34.2%), α-pinene (6.6%), sabinene (6.1%) and β-bisabolene (5.8%) being the main components. Twenty-seven components were identified in leaf REO, with the main components being camphor (31.9%), borneol (12.2%), 1,8-cineole (11.3%) and myrcene (10.7%). The BEOs isolated from leaves (EC₅₀ value of 1.43 mg/mL) and fruit (EC₅₀ value of 3.74 mg/mL) showed stronger antioxidant activity than REO (EC₅₀ value of 5.00 mg/mL) during an incubation time of 20 min. The results obtained for the bay and rosemary EOs from Montenegro highlight their potential not only for culinary purposes but also as a source of antioxidants and for use in the food and pharmaceutical industries. Full article

Functional foods and the nutritional value of products are now very relevant for consumers. Additionally, interest in biodegradable components made from natural products has recently increased. The aim of this study was to determine the effect of different package types (with addition of films made of biopolymers enriched with natural extracts of ginger, cinnamon, and turmeric) on antioxidative properties of conventional, organic, and wild-grown raspberry fruit during frozen storage for 1-, 4-, 8-, and 12-month periods. The content of total polyphenol and anthocyanins, as well as antioxidant activity, were studied. Additionally, structural (FT-IR and UV-Vis), mechanical (tensile strength and elongation), and surface wetting angle tests of used films were studied. In all crop types, polyphenol content increased at 8 and 12 months of frozen storage in packages with an addition of biopolymer films. There was a statistically significant increase in the amount of total polyphenols in the last month of storage in the package with films with added cinnamon extract. In contrast, packages with ginger-enriched films contributed to an increase in polyphenols only in organically grown and wild raspberry fruit at the 4th, 8th, and 12th month and 8th and 12th month, respectively. The addition of films with turmeric extract increased the polyphenol content in the 8th month of frozen storage of organically grown raspberry fruit and in the 12th month of wild-grown raspberry. During frozen storage of wild raspberry fruit, an increase in anthocyanin content was observed in all cases in the 12th month. This result was almost twice as high as in the control sample. The greatest rise in the content of anthocyanins occurred when packages with films with turmeric extract were applied. Tendencies presented by this paper are not clear-cut in cases of antioxidant activity of analysed raspberries. A common feature was that, in general, after 12 months of storage, raspberries from all crops had higher and statistically significant antioxidant values compared to the first month of storage. Studies on the biocomposite films have shown that the structure of chitosan and alginate polymers was partly decomposed after 12 months, and the natural extracts and raspberry crops used had different effects on the mechanical properties of the biocomposites films. Based on the results of research that has been carried out, it can be concluded that it has not been clearly proven whether the addition of polysaccharide films to packages contributes to the increase in bioactive compounds during frozen storage. Full article

Peach (Prunus persica), a significant economic fruit tree in the Rosaceae family, is extensively cultivated in temperate and subtropical regions due to its abundant genetic diversity, robust adaptability, and high nutritional value. Originating from China over 4000 years ago, peaches were introduced to Persia through the Silk Road during the Han Dynasty and gradually spread to India, Greece, Rome, Egypt, Europe, and America. Currently grown in more than 80 countries worldwide, the expansion of peach cultivation in Egypt is mainly due to the development and utilization of peach varieties with low chilling requirements. These varieties exhibit unique phenotypic characteristics such as early maturity, reduced need for winter cold temperatures, low water requirements, and high economic value. In this study, a systematic analysis was conducted on the genetic characteristics and kinship relationships of peaches with low chilling requirements in Egypt. We conducted a comprehensive evolutionary and Identity-by-Descent (IBD) analysis on over 300 peach core germplasm resources, including Egyptian cultivars with low chilling requirements, to investigate their origin and genetic characteristics. The evolutionary analysis revealed that ‘Bitter almond’ is closely related to China’s wild relative species Prunus tangutica Batal, while ‘Early grand’ shares one branch with Chinese ornamental peach cultivars, and ‘Nemaguard’ clusters with some ancient local varieties from China. The IBD analysis also indicated similar genetic backgrounds, suggesting a plausible origin from China. Similarly, the analysis suggested that ‘Swelling’ may have originated from the Czech Republic while ‘Met ghamr’ has connections to South Africa. ‘Desert red’, ‘Early swelling’, and ‘Florida prince’ are likely derived from Brazil. These findings provide valuable insights into the genetic characteristics of Egyptian peach cultivars. They offer a significant foundation for investigating the origin and spread of cultivated peaches worldwide and serve as a valuable genetic resource for breeding low chilling requirement cultivars, which is of considerable significance for the advancement of peach cultivation in Egypt. Full article

Daucus capillifolius Gilli is a rare annual wild herb grown in Libya. It belongs to the Apiaceae family, which is one of the largest flowering plant families. Plants of this family are outstanding sources of various secondary metabolites with various biological activities. A UPLC-ESI-MS/MS analysis of different extracts of in vivo and in vitro tissues of Daucus capillifolius together with the fruit extract of the cultivated plant in both ionization modes was carried out for the first time in the current study. Our results reveal the tentative identification of eighty-seven compounds in the tested extracts, including thirty-two phenolic acids and their derivatives; thirty-seven flavonoid glycosides and aglycones of apigenin, luteolin, diosmetin, myricetin and quercetin, containing glucose, rhamnose, pentose and/or glucuronic acid molecules; seven anthocyanins; six tannins; three acetylenic compounds; and three nitrogenous compounds. The tentative identification of the above compounds was based on the comparison of their retention times and ESI-MS/MS fragmentation patterns with those previously reported in the literature. For this Apiaceae plant, our results confirm the presence of a wide array of secondary metabolites with reported biological activities. This study is among the first ones to shed light on the phytoconstituents of this rare plant. Full article

Innovation in the beer market focuses on research into the different ingredients that make up this popular and sought-after beverage. Some of these innovations have focused on the use of autochthonous ingredients, which bring added value to products, reduce production costs and environmental impact, and provide beers with special organoleptic characteristics. This study focuses on the use of autochthonous ingredients from the Community of Madrid and the design of four recipes that are suited to these ingredients and consumer demand. In terms of the ingredients used, the micromalting of barley grown in the CM was carried out, while hops (Cascade variety for bittering hops and wild hops Rayo verde Tajuña and Torreta Tajuña for aroma) and yeast strains (Saccharomyces cerevisiae G 520 and G 354) were selected from previous studies, which were also sourced from the CM. For the barley malt, parameters such as protein content were determined, which was at the maximum limit but did not affect the final quality of the beers. The content of α- and β-acids in the hops was also determined, being higher in the Cascade variety. Different physicochemical analyses (GC, aromatic compounds, bitterness, colour, SO₂, polyphenols, and antioxidant capacity) were carried out to determine the quality of the beers obtained from the four recipes designed, as well as tastings by a panel of experts and consumers. Finally, two recipes were rated the best by a panel of experts and consumers, where the cereal, hop, and fruit flavours stood out for their aromatic profile. Full article

Raspberry (Rubus idaeus L.) is a fruit of great interest due to its aroma, nutritional properties, and the presence of many bioactive compounds. However, differences among cultivation systems can affect its composition and, consequently, its potential bioactivity. Herein, for the first time, raspberries grown in an aeroponic system were investigated for their chemical profile and antioxidant and anti-inflammatory activity, as well as their enzyme (α-glucosidase and pancreatic lipase) inhibitory properties in comparison to wild and conventionally cultivated fruits. High-performance liquid chromatography coupled with diode array detection (HPLC-DAD) analyses revealed the presence of gallic acid, caffeic acid, chlorogenic acid, p-coumaric acid, ferulic acid, rutin, and catechin in all the samples. The extracts exhibited in vitro anti-inflammatory activity (inhibition of nitric oxide production) regardless of the cultivation method. Of particular interest is the ability of raspberries to inhibit pancreatic lipase. With the exception of the β-carotene bleaching test, the raspberries grown in conventional and aeroponic systems were more active in terms of antioxidants than wild fruits, as evidenced by the ABTS (IC₅₀ in the range 1.6–3.4 μg/mL), DPPH (IC₅₀ in the range 8.9–28.3 μg/mL), and FRAP tests (24.6–44.9 μM Fe(II)/g). The raspberries from aeroponic cultivation were generally able to exert the same bioactivity as those obtained from both conventionally cultivated and wild fruits, supporting the consideration that in the future, this technology could reshape agriculture by mitigating resource constraints, fostering sustainable practices and increasing yields. Full article

The fruits of Solanum torvum Swartz, a wild relative of eggplant, are consumed as a wild vegetable in tropical regions of Africa, Asia, and South America. In traditional Chinese medicine, it is believed to have anti-inflammatory and sedative effects. In the Philippines, water decoction is used to treat hyperactivity disorder. Twenty-two steroidal saponins were isolated and purified from the fruits grown in Yunnan, China, including six new compounds: torvosides U–Z (1–6). During drying and cooking, the saponins may undergo transformation, resulting in small amounts of sapogenins. These transformations can include dehydration of hydroxyl groups at position C22, formation of double bonds at position 20, 22 or 22, 23, and even formation of peroxide products. Saponin compounds torvoside X (4), torvoside Y (5), torvoside A (7), and (25S)-3-oxo-5α-spirostan-6α-yl-O-β-d-xylopyranoside (20), which are glycosylated at C-6, showed certain anti-epileptic activity in a pentylenetetrazole-induced zebrafish seizure model. No antiproliferative activity was detected when tested on the cancer cell line HepG2, and no hepatotoxic effect was noted on normal liver cell line LO2. Full article

The five independent countries of Central Asia, namely Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, and Uzbekistan, contain one of the richest areas in the world for the specific and intraspecific diversity of temperate fruit and nut tree species. Research was carried out via the collaboration of national research and education institutes with local community-based agencies and farmer communities. Raw data (2014 observations) for almond, apple, apricot, cherry plum, currant, grapevine, pear, pomegranate, and walnut were collected at the household (HH) level across the five countries: Uzbekistan, Kazakhstan, Tajikistan, Turkmenistan, and Kyrgyzstan. A set of models was used, including household variety richness as the dependent variable, to understand the influence of socio-ecological variables on the amount and distribution of crop varietal diversity in the farmers’ production systems. Four variables were included as explanatory variables of variety richness (fixed factors): ecoregion, ethno-linguistic group, management, and abiotic stress. The results show clear evidence that abiotic stress determines a higher richness of intra-specific diversity in the form of local varieties grown by farmers living in climatically unfavorable areas. The results for the studied ecoregions follow the same trend, with ecoregions with harsher conditions displaying a higher positive correlation with diversity. Mild environments such as the Central Asian riparian woodlands show an unexpectedly lower diversity than other harsher ecoregions. Ethno-linguistic groups also have an effect on the level of varietal diversity used, related to both historic nomadic practices and a culture of harvesting wild fruit and nuts in mountainous areas. The home garden management system hosts a higher diversity compared to larger production systems such as orchards. In Central Asia, encouraging the cultivation of local varieties of fruit and nut trees provides a key productive and resilient livelihood strategy for farmers living under the harsh environmental conditions of the region while providing a unique opportunity to conserve a genetic heritage of global importance. Full article

Lepista sordida is a type of high-quality rare edible and medicinal mushroom, and its research boom is just beginning. More than 80 million tons of grain crop residues are produced each year in Heilongjiang Province. To realize the exploration and utilization of wild L. sordida mushrooms and also provide a theoretical support for the high-value utilization of these resources in Heilongjiang Province, we evaluated the cultivation of L. sordida mushrooms using rice straw, corncob, and soybean straw as substrates. L. sordida grew on all three substrates, and the biological efficiency and yield of the mushrooms grown on soybean straw and corncob were 32.33 ± 1.78% and 4.20 ± 0.23 kg m⁻², and 30.15 ± 0.93% and 3.92 ± 0.12 kg m⁻², respectively, which increased by 9.38% and 2.08% compared with that on the rice straw substrate with 3.84 ± 0.12 kg m⁻² and 29.56 ± 0.89%. The time it took for the mycelia to colonize and initiate primordia on the soybean straw substrate was 22.33 ± 0.58 d and 19.67 ± 0.58 d, respectively, which was delayed by 2 d and 3 d compared with that on the rice straw substrate with 20.67 ± 2.08 d and 16.33 ± 0.58 d, respectively. The fruiting bodies grown on corncob and soybean straw substrates were relatively larger than those on the rice straw substrate. The highest amount of crude protein was 57.38 ± 0.08 g 100 g⁻¹, and the lowest amount of crude polysaccharide was 6.03 ± 0.01 g 100 g⁻¹. They were observed on mushrooms collected from the corncob substrate. The contents of the heavy metal mercury, lead, arsenic, and cadmium in the fruiting bodies grown on each substrate were within the national safety range. Full article

Rosehip is a valuable fruit species in particular for rural populations. In this study, fruit quality parameters of 15 wild grown rosehip ecotypes naturally obtained from seeds in the Aegean Region of Türkiye were determined. The fruit weight, fruit firmness, fruit flesh ratio, fruit shape index, fruit skin color (chroma), soluble solid content, vitamin C, total phenolic, total carotenoid, total anthocyanin, total flavonoid and antioxidant capacity were investigated. The results showed great diversity among ecotypes. The fruit weight, fruit firmness, fruit flesh ratio, fruit shape index, fruit skin color (chroma) and soluble solid content were between 2.28 and 3.29 g, 4.70 and 7.12 N, 69.34 and 81.67%, 0.97 and 1.07, 53.04 and 60.71 and 18.87 and 21.28%, respectively. The total antioxidant capacity was found to be 15.78–28.17 mg AAE/g in a DPPH assay. The vitamin C content of rosehip fruits was measured as 507–621 mg/100 g. Among ecotypes, A-15 gave the biggest fruits, A-1 had the highest soluble solid content and A-13 had the highest vitamin C content. These results suggested that some ecotypes showed more potent bioactive properties than other ecotypes, mainly related to the variations in the antioxidant capacity and bioactive content between ecotypes. Overall, this study provides additional insight into investigating the genotype exhibition of multifunctional bioactive properties. Full article

Litchi (Litchi chinensis Sonn.) is a highly valuable fruit crop that is widely grown in tropical and subtropical areas of the world. Studying its genetic diversity and population structure is critical for effective conservation and breeding programs. In this study, we developed 150 single-nucleotide polymorphism (SNP) markers that were evenly spaced across litchi genome and applied them to the evaluation of the genetic diversity of 84 litchi accessions, including old cultivars, modern cultivars, hybrids from known parents and wild accessions. Ninety-one SNP markers, showing high levels of polymorphism and high genotyping success rates, were used for further analysis. The newly developed SNP markers captured a relatively higher level of genetic diversity (He = 0.364) in litchi cultivars and could be successfully applied for the identification of synonymous cultivars and hybrids with close genetic backgrounds. Cluster analysis grouped all genotypes into three clusters that showed perfect association with their fruit maturation period, among which wild accessions clustered with their corresponding domesticated cultivars, and hybrids from different parent combinations showed different inheritance tendencies. Our study not only provided a set of efficient SNP markers for future genetic research, but also laid an important foundation for the conservation and genetic breeding of litchi. Full article