Search Results (136,937)

Background: Torque teno virus (TTV) is a ubiquitous, non-pathogenic component of the human virome whose role in people living with HIV (PLWH), particularly during antiretroviral therapy (ART)-mediated immune reconstitution, remains unclear. This systematic review aimed to synthesize available evidence on TTV viral load in PLWH, focusing on its relationship with immunological markers. Methods: This systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines. A comprehensive literature search was conducted in MEDLINE, Web of Science, and Scopus in January 2026 to identify studies assessing plasma TTV viral load before and/or during ART and reporting immunological outcomes. Eligible studies included prospective and retrospective longitudinal studies, cross-sectional studies, and mixed designs assessing plasma TTV viral load in relation to ART status and immune recovery markers. Results: Thirteen studies (n = 1700 PLWH) were included, predominantly observational and conducted in adult populations. Most studies (76.9%) reported a significant inverse association between TTV viral load and CD4 T-cell count, while all studies assessing HIV viral load found a direct correlation with TTV levels. An inverse relationship with the CD4/CD8 ratio was consistently observed where evaluated. Higher TTV loads were reported in ART-naïve individuals and in those with advanced immunosuppression, with longitudinal studies indicating a general decline during ART. Overall, methodological heterogeneity and moderate risk of bias were common. Conclusions: TTV viral load shows a consistent inverse association with CD4 cell count and may reflect global immune dysfunction in PLWH beyond conventional markers. However, its clinical utility remains investigational due to the heterogeneity in the study design, limited data on longitudinal dynamics, and lack of standardized assays and thresholds. Full article

Calcrete-type uranium prospectivity prediction is challenged by the strong heterogeneity of multi-source geoscientific raster datasets, weak anomaly responses, and the lack of explicit heterogeneous information organization in conventional deep learning models. In this study, the Yilgarn Craton of Western Australia was selected as the study area, and a geology-guided fixed-group fusion ResUNet model (GGF-ResUNet) was developed based on 12-channel multi-source geoscientific raster datasets. At the input stage, the evidence layers were divided into four fixed geoscientific proxy groups according to their data modality and geological interpretation, namely gravity, aeromagnetic, radiometric, and geochemical groups, and intra-group channel weighting together with inter-group gating was introduced to enhance the hierarchical representation and adaptive fusion of heterogeneous information. Ablation results showed that GGF-ResUNet achieved better performance than the baseline ResUNet, with AUC increasing from 0.9340 to 0.9740 and F1-score improving from 0.7264 to 0.8356. Further comparative experiments with Attention U-Net, U-Net, SegNet, and FCN showed that GGF-ResUNet achieved comparatively better quantitative performance and more spatially coherent prediction results under the current experimental setting. Without substantially increasing model complexity, the proposed method improves the representation and integration of heterogeneous geoscientific information and provides a feasible technical pathway for calcrete-type uranium prospectivity prediction under weak-anomaly conditions. Full article

Lean Construction (LC) aims to enhance efficiency, minimize waste, and increase value in construction. However, its implementation is inconsistent, especially in developing countries like Jordan, due to a lack of assessment frameworks. This study examines the Lean Construction Knowledge Framework (LCKF) to assess LC capability in Jordan’s construction sector. A qualitative multiple-case study was conducted with three selected construction companies in Jordan, using semi-structured interviews and direct observation. A three-point scoring system evaluated 12 LCKF variables across three knowledge dimensions based on evidence from interviews, observations, and documents. This exploratory study found that most LCKF variables were partially implemented or absent. Effective stakeholder communication was strong, while flow tracking, visual management, and Key Performance Indicators (KPIs) were descriptive and not improvement-oriented. Deficits included a lack of formal training in waste reduction, visualization, and pull-based control, as well as inconsistent adoption of pull planning. A cross-case analysis confirmed shared gaps among the companies, indicating broader challenges in similar contexts. Based on the scoring results and follow-up interviews feedback, the LCKF was translated into a Jordan-specific roadmap that distinguishes between established practices, short-term enhancements, and long-term capability development. The study contributes by highlighting that LC implementation depends on tool adoption, organizational routines, learning mechanisms, and staged capability building. Full article

Terra rossa (red soils) developed over carbonate terrains represent an unconventional yet potentially valuable secondary source of rare earth elements (REEs) due to enrichment in Fe–Al-rich weathering products. In this study, the hydrometallurgical recovery of REEs from terra rossa samples collected from the Seydişehir–Akseki region (Türkiye) was systematically investigated through acid leaching. Sulfuric acid (H₂SO₄), hydrochloric acid (HCl), and nitric acid (HNO₃) were comparatively evaluated as lixiviants under optimized conditions. A Taguchi L16 orthogonal experimental design was used to assess the effects of acid concentration (1–4 M), temperature (25–95 °C), leaching duration (60–480 min), and solid-to-liquid ratio (1:10–1:40). All three acids achieved high REE recoveries under optimized conditions (4 M, 95 °C, 480 min, and 1:40 S/L). HCl exhibited the highest overall efficiency, yielding recoveries of 93–98%, followed by H₂SO₄ (89–96%) and HNO₃ (88–97%). Statistical analyses indicated that acid type and concentration were the main factors controlling REE dissolution, followed by temperature and leaching duration. Overall, the results confirm that terra rossa deposits constitute an underexplored secondary REE resource and demonstrate that optimized acid leaching is an effective approach for the valorization of weathering-derived materials. Full article

Baseline information on terrestrial wildlife communities and their activity patterns is essential for protected-area management, but such information remains limited for Hunan Zhangjiajie Giant Salamander National Nature Reserve, where conservation attention has historically focused on the Chinese giant salamander and associated aquatic ecosystems. From March 2024 to August 2025, we conducted a camera-trap survey in broad-leaved and coniferous forest habitats of the reserve to document terrestrial mammals and ground-dwelling birds, evaluate taxonomic completeness, and describe diel and seasonal activity patterns. Across 43 camera-trap stations and 16,314 effective camera-trap days, we recorded 59 wildlife species, including 18 mammals and 41 ground-dwelling birds. The assemblage included nationally protected, threatened, and Chinese endemic species, indicating that the reserve’s forest habitats support important terrestrial biodiversity in addition to its aquatic conservation target. Taxonomic completeness curves suggested that the current survey captured most camera-detectable mammal and ground-dwelling bird taxa under the present sampling design, although the results should not be interpreted as a complete inventory of the reserve’s total vertebrate diversity. Annual diel activity analysis of 11 focal species showed clear temporal differentiation among ecological groups: small and medium-sized carnivores were mainly nocturnal, ground-dwelling birds, and red-hipped squirrel were primarily diurnal, and ungulates showed mixed or crepuscular-to-nocturnal tendencies. Seasonal analyses based on bioclimatic periods showed interspecific differences in activity-density distributions between the cool-dry and warm-wet seasons. However, peak-shift reliability analysis indicated that most focal species retained broadly similar main activity peaks across seasons; masked palm civet was the only species showing reliable seasonal displacement of its main activity peak. Pairwise temporal overlap analyses described temporal co-occurrence patterns among selected sympatric species but should not be interpreted as evidence of direct interaction or niche differentiation. Overall, this study provides baseline data on camera-detected terrestrial vertebrates in the reserve and supports long-term monitoring, forest habitat management, and disturbance control for terrestrial mammals and ground-dwelling birds. Full article

The Arabidopsis thaliana Heat Shock Protein-Related (AtHSPR) gene participates in plant growth and abiotic stress tolerance, while its role in biotic stress resistance remains unclear. Here, we report that the athspr mutant is sensitive to Pseudomonas syringae pv. tomato (Pst) DC3000, whereas over-expression of AtHSPR enhances the defense of Arabidopsis against the pathogen. AtHSPR expression was induced by treatment with Pst DC3000, flg22, or salicylic acid (SA). Transcriptome analysis showed that mutation of AtHSPR changed the expression patterns of genes associated with defense response, oxidation–reduction, and SA responses, as well as transcription factors. The biochemical evidence revealed that AtHSPR interacted with Thimet Oligopeptidase 1 (TOP1), which modulated the SA-mediated immune response. Co-expression of AtHSPR and TOP1 showed that the TOP1 protein, normally located in the chloroplasts, gathered around the nucleus in response to a pathogen. After pathogen treatment, dynamic tubular projections (stromules) were present, extending from the chloroplasts toward the nucleus, and TOP1 was observed in the nucleus, together with AtHSPR. The top1athspr double mutant had lower SA levels and was more sensitive to pathogens than the top1 and athspr single mutants. Taken together, our results demonstrated that the interaction between AtHSPR and TOP1 plays a positive role in SA-mediated plant resistance against Pst DC3000. Full article

Legume cultivation offers a chance for agricultural development on lands that have been abandoned over the years. In this study, simple agronomic indicators on the growth and yield of faba bean (Vicia faba L.), pea (Pisum sativum L.), and white lupin (Lupinus albus L.) were assessed on the abandoned agricultural lands of Foloi Plateau in Western Greece. Field trials were conducted from October 2023 to July 2025, and the legumes were grown either according to the false seedbed concept or with conventional seedbed preparation practices (direct sowing). The false seedbed involves pre-sowing weed control following initial seedbed preparation, and in these trials, it suppressed weed density by 62–77%. The Normalized Difference Vegetation Index (NDVI) of faba bean and pea increased by 13% on the false seedbed plots, while white lupin NDVI was not affected by treatments (p ≥ 0.05). Destructive crop biomass measurements were in accordance with NDVI assessments. Faba bean and pea seed yield demonstrated an increase of 17% and 23%, respectively, in the false seedbed plots compared to direct sowing plots. White lupin seed yield was not significantly affected by false seedbed (p ≥ 0.05). This study provides preliminary evidence supporting the use of legume crops as a component of sustainable agricultural revitalization in the Foloi region. However, further research is required to optimize legume cultivation on the abandoned lands of the wider region as a first step towards the agricultural revitalization in the Plateau. Full article

Pancreatic cancer (PC) remains one of the malignancies with the most unfavorable prognosis and limited treatment options. The lack of biomarkers for early diagnosis and the asymptomatic nature of the disease contribute to delays in diagnosis and high mortality rates. In recent years, the role of the human microbiota in cancer biology has become increasingly significant, and the oral microbiota in particular has been found to be involved in the pathogenesis and prognosis of several neoplasms. This review summarizes the current evidence relating the salivary microbiota to PC in three key areas: screening and diagnostic potential, pathophysiology and tumor progression, as well as presenting prognostic implications and potential influence on therapy. With regard to early diagnosis, it has been reported that patients with PC have reduced levels of Neisseria elongata (N. elongata) and Streptococcus mitis (S. mitis) and elevated levels of Granulicatella adiacens. Several studies have shown that bacteria present in the saliva can migrate from the oral cavity to pancreatic tissue via hematogenous or enteric routes, where they may actively contribute to tumor development and progression. In particular, it has been shown that Porphyromonas gingivalis (P. gingivalis) and Veillonella atypica (V. atypica) translocate from the mouth to pancreatic tumors, promoting carcinogenesis by inducing a pro-inflammatory tumor microenvironment. Furthermore, some studies have identified certain species associated with prognosis and response to PC treatment. Despite the encouraging results, differences in study methodology, the lack of standardized methods and the scarcity of longitudinal data currently hinder clinical application. Large-scale, multi-omics prospective studies are needed to clarify causality and validate their clinical utility. Overall, the salivary microbiota represents a promising and non-invasive tool for improving early diagnosis, understanding prognosis and enhancing the management of PC. Full article

Network latency remains a fundamental bottleneck in vehicle teleoperation, inducing instability and performance degradation in conventional control methods, while predictive techniques like the Smith Predictor offer a theoretical solution, their efficacy is often compromised by unmodelled dynamics and real-world disturbances. This paper presents the first experimental validation of the Successive Reference-Pose Tracking (SRPT) architecture. By streaming future reference poses rather than direct steering commands, SRPT leverages an onboard Nonlinear Model Predictive Controller to compute optimal vehicle actions while inherently accounting for dynamic constraints and network delays. Real-world human-in-the-loop experiments were conducted with four drivers on a test track featuring cornering, double lane-change, and slalom manoeuvres. Quantitative comparisons at 10 km/h across four modes—manual driving, direct teleoperation, a Smith Predictor, and SRPT—demonstrate that SRPT significantly outperforms other teleoperation methods, reducing cross-track error by up to 66% and yielding smoother, more stable control inputs. Furthermore, SRPT uniquely maintained stability during a proof-of-concept trial at 13 km/h, where it proactively moderated vehicle speed to respect actuator limits—a critical safety behavior absent in other modes. This work provides the first tangible evidence that SRPT is a robust and superior framework for delay-resilient vehicle teleoperation in real-world conditions. Full article

This study examines the structural relationships among vendor competency, perceived sanitation and safety, high-value gastronomy experiences, and behavioral intentions in Bangkok’s street food tourism. Using survey data and PLS-SEM, the results indicate that vendor competency significantly enhances both perceived sanitation and safety and high-value gastronomy experiences. Perceived sanitation and safety further strengthens experiential value and partially mediates this relationship. However, contrary to conventional expectations, high-value gastronomy experiences exhibit a significant negative effect on behavioral intentions, suggesting a novelty-seeking paradox in exploratory tourism contexts. This study contributes in three ways: (1) by positioning vendor competency as a foundational driver of experiential value in informal food settings, (2) by integrating sanitation and safety into experiential value formation, and (3) by challenging the linear satisfaction–loyalty assumption through evidence of paradoxical tourist behavior. The findings offer theoretical and managerial implications for gastronomy tourism and destination management. Full article

Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by impaired social interaction, communication deficits, and repetitive behaviors. Recent research highlights the role of the gut microbiota in ASD pathophysiology, particularly through the microbiota–gut–brain axis. The microbiota may influence neurodevelopment via multiple signaling pathways, including the GABAergic and serotonergic systems, as well as the mTOR signaling pathway. This analytical review examines current evidence on microbiota alterations in ASD and evaluates microbiota-targeted strategies for diagnosis and treatment, focusing on fecal microbiota transplantation (FMT), probiotics, and diet-based therapeutic approaches. The review also provides a critical appraisal of the microbiota–gut–brain axis in the context of autism spectrum disorder. Full article

The increasing prevalence of antimicrobial resistance (AMR) has promoted the development of advanced biomaterials capable of overcoming the limitations of conventional antibiotics. In this context, metal nanoparticle hybrid hydrogels (MNHHs) have emerged as multifunctional platforms that integrate the high water-retention capacity and biocompatibility of hydrogels with the antimicrobial properties of metallic nanoparticles (MNPs). This review critically analyzes recent advances in the design, physicochemical properties, antimicrobial mechanisms, and biomedical applications of these systems. Current evidence demonstrates that MNHHs can achieve antimicrobial efficiencies above 98–99%, with minimum inhibitory concentrations as low as 0.78 µg mL⁻¹ and inhibition zones of up to 25 mm against clinically relevant pathogens. Furthermore, the incorporation of MNPs significantly improves the mechanical properties of hydrogels and enables controlled and sustained metal ion release for periods of up to 14 days. Despite these promising results, important challenges remain regarding cytotoxicity, release control, the lack of experimental standardization, and the limited understanding of long-term biological effects. Overall, MNHHs represent a promising strategy for infection control, regenerative medicine, and controlled drug delivery; however, their clinical translation still requires the development of reproducible, safe, scalable, and highly biocompatible systems. Full article

Plot combine harvesters are specialized machines used in breeding trials, germplasm evaluation, and small-batch seed harvesting. Compared with conventional field combine harvesters, they have higher requirements for sample independence, grain integrity, seed purity, low residual grain, rapid plot switching, and plot-level data reliability. However, existing studies remain relatively fragmented, and many studies mainly focus on individual components, whereas analyses of whole-machine coordination, residual-grain control, crop adaptability, and data integration remain insufficient. This paper presents a structured review of the research progress in plot combine harvesters from an agricultural-engineering perspective, covering representative international and domestic models, headers, threshing and separation systems, cleaning systems, residual-seed removal devices, simulation methods, intelligent monitoring, and seed-quality sensing. Existing evidence indicates that plot combine harvesters are developing toward whole-machine low-residue design, coordinated threshing–cleaning–conveying optimization, standardized evaluation methods, sample identification, data traceability, and long-term field validation under continuous multi-plot harvesting conditions. Key challenges include coordinating small-batch intermittent material flow, controlling residual grain during frequent plot switching, balancing threshing completeness with seed protection, improving adaptability to different crops and breeding materials, and validating intelligent sensing technologies under field conditions. This paper provides an engineering reference for improving the mechanization, precision, and intelligence of breeding-trial harvesting equipment. Full article

Background: Immune checkpoint inhibitors (ICIs) are standard treatment for melanoma and non-small cell lung cancer (NSCLC), yet evidence on their effectiveness in older adults remains limited due to underrepresentation in clinical trials. This study assessed long-term, age-stratified outcomes of ICI treatment in real-world clinical practice. Methods: This nationwide observational study used data from the Netherlands Cancer Registry on patients with synchronous stage IV melanoma or NSCLC who received first-line ICIs between 2018 and 2023. Melanoma treatments included nivolumab plus ipilimumab or anti-PD-1 monotherapy; NSCLC treatments included pembrolizumab with or without chemotherapy. Primary outcomes were five-year overall survival (5-yr OS) and three-year conditional survival (3-yr CS), stratified by age. Results: A total of 11,140 patients were included, consisting of 583 patients with melanoma and 10,557 with NSCLC. In the melanoma population, 5-yr OS was 43.8%. Patients aged ≥ 75 years had a 5-yr OS of 30.8% and a 3-yr CS of 58.7%. In NSCLC treated with pembrolizumab monotherapy, 5-yr OS was 23.1%; among patients aged ≥ 75 years, 5-yr OS was 15.6% and 3-yr CS was 46.6%. Pembrolizumab combined with chemotherapy resulted in a 5-yr OS of 14.6%, with corresponding 5-yr OS of 8.4% and 3-yr CS of 35.5% in patients aged ≥ 75 years. Conclusions: This registry-based analysis suggests that ICI are associated with durable long-term survival in real-world patients with stage IV melanoma or NSCLC, including selected older adults. These findings are in line with outcomes of clinical trials, but further research is needed on predictors of ICI effectiveness in the older population. Full article

Background/Objectives: Postoperative monitoring of microvascular free flaps is critical for early detection of vascular complications and flap survival. Nurses play a central role in this process; however, qualitative evidence on their experiences and challenges remains limited. This study explored nurses’ experiences in free tissue flap care to identify clinical practices, challenges, and improvement needs. Methods: A phenomenological qualitative design was used. Data were collected through semi-structured interviews with nine nurses experienced in free tissue flap care, recruited via purposive and snowball sampling. Interviews were conducted online and lasted 30–45 min. Data were analyzed using content analysis with MAXQDA 2025. Inter-researcher reliability was 97%. Results: The findings were categorized into four main themes and seventeen subthemes: (1) clinical monitoring and evaluation in the care process, (2) challenges and difficulties, (3) emotional and professional reflections, and (4) suggestions for improving care. Nurses reported that flap care requires intensive monitoring, rapid decision-making, and close collaboration with physicians, especially within the first 24–48 h. Monitoring was largely based on observation and experience due to the lack of standardized protocols. Major challenges included high workload, frequent assessments, and donor site management. Emotional burden, stress, and responsibility were also prominent. Conclusions: Free flap care is a complex and demanding process for nurses. The lack of standardized monitoring tools and protocols is a key gap. Developing structured tools, improving training, and strengthening multidisciplinary collaboration may enhance patient safety and care quality. Full article