Search Results (1,874)

Small ruminant production is increasingly affected by heat stress, with recent heat waves highlighting growing economic and welfare-related challenges. Chronic exposure to elevated temperatures disrupts thermoregulation, reduces feed intake, slows growth, compromises meat quality, and increases mortality. This study evaluated the effects of chromium-yeast supplementation at different doses and timepoints on physiological and molecular stress biomarkers in heat-stressed lambs. Forty-eight clinically healthy 6-month-old Katahdin lambs (average weight 20 ± 2.9 kg) were assigned to a 2 × 4 factorial design, with two ambient temperature conditions (heat stress [HS] and thermoneutral [TN]) and four levels of dietary Cr-yeast (0, 0.2, 0.4, and 0.8 mg/kg of dry matter intake). Lambs were housed individually in pens (1.2 × 2.5 m), with ad libitum access to water, and fed a 50:50 corn silage and concentrate diet (excluding mineral premix) twice daily. Blood samples were collected at days 0, 30, and 60 to evaluate plasma cortisol and the expression of hsp60 and hsp70. Chromium bioavailability was assessed by blood levels using absorption chromatography, and glucose clearance was measured at the end of the experiment. Significant reductions in cortisol and hsp70 expression were observed after 30 days of Cr-yeast supplementation under HS conditions (p < 0.05), particularly at the highest dose. For hsp60, a significant reduction was observed at the highest dose on day 30 under HS (p < 0.05). These effects were not sustained on day 60 (p > 0.05). No significant differences were detected under TN conditions (p > 0.05). These findings suggest that Cr-yeast may offer short-term physiological and cellular protection against chronic heat stress in lambs. Full article

Despite increasing evidence that cigarette smoke is a significant source of indoor fine particulate matter (PM_2.5), quantitative emission factors (EFs) for PM_2.5 and its toxic chemical composition in mainstream (MS) and sidestream (SS) smoke are still not well defined. In this study, we employed a custom-designed chamber to separately collect MS (intermittent puff) and SS (continuous sampling) smoke from eleven cigarette models, representing six brands and two product types, under controlled conditions. PM_2.5 was collected on quartz-fiber filters and analyzed for carbon fractions (using the thermal–optical IMPROVE-A protocol), nine water-soluble inorganic ions (by ion chromatography), and twelve trace elements (via ICP-MS). SS smoke exhibited significantly higher mass fractions of total analyzed species (84.7% vs. 65.9%), carbon components (50.6% vs. 44.2%), water-soluble ions (17.1% vs. 13.7%), and elements (17.0% vs. 7.0%) compared to MS smoke. MS smoke is characterized by a high proportion of pyrolytic organic carbon fractions (OC₁–OC₃) and specific elements such as vanadium (V) and arsenic (As), while SS smoke shows elevated levels of elemental carbon (EC1), water-soluble ions (NH₄⁺, NO₃⁻), and certain elements like zinc (Zn) and cadmium (Cd). The toxicity-weighted distribution indicates that MS smoke primarily induces membrane disruption and pulmonary inflammation through semi-volatile organics and elements, whereas SS smoke enhances oxidative stress and cardiopulmonary impairment via EC-mediated reactions and secondary aerosol formation. The mean OC/EC ratio of 132.4 in SS smoke is an order of magnitude higher than values reported for biomass or fossil-fuel combustion, indicative of extensive incomplete combustion unique to cigarettes and suggesting a high potential for oxidative stress generation. Emission factors (µg/g cigarette) revealed marked differences: MS delivered higher absolute EFs for PM_2.5 (422.1), OC (8.8), EC (5.0), Na⁺ (32.6), and V (29.2), while SS emitted greater proportions of NH₄⁺, NO₃⁻, Cl⁻, and carcinogenic metals (As, Cd, Zn). These findings provide quantitative source profiles suitable for receptor-oriented indoor source-apportionment models and offer toxicological evidence to support the prioritization of comprehensive smoke-free regulations. Full article

Saline–alkaline water resources are globally widespread, and their rational development offers significant potential to alleviate freshwater scarcity. Saline–alkaline water aquaculture farming not only affects fish growth and survival but also impairs reproductive and developmental functions. Largemouth bass (Micropterus salmoides), an economically important fish, has demonstrated excellent high tolerance to such environments, in order to investigate the effects of alkaline water aquaculture environments on its growth performance, sex hormone levels, gonadal development, and molecular adaptation mechanisms. In this study, largemouth bass were chronically exposed to freshwater (0.55 mmol/L), low alkalinity (10 mmol/L), or high alkalinity (25 mmol/L) and cultured for 80 days. Alkalinity exposure more severely impacted the growth rate of females. High alkalinity significantly increased the hepatosomatic index and decreased the gonadosomatic index in both sexes; moreover, it induced oxidative stress in both sexes, evidenced by reduced superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (TAOC) levels and elevated malondialdehyde (MDA) content. Furthermore, the levels of sex hormones Serum estradiol (E2), 11-ketotestosterone (11-KT), and testosterone were significantly reduced, accompanied by either an elevated ratio of primary oocytes and follicular atresia, or by reduced spermatogenesis. Apoptotic signals appeared in gonadal interstitial cells, with upregulated expression of genes P53, Bax, Casp3, and Casp8. Ultrastructural damage included fewer mitochondria and cristae blurring, further indicating tissue damage causing dysfunction. Transcriptome results showed that oxidative stress damage and energy metabolism imbalance caused by carbonate alkalinity were key to the delayed gonadal development, which was mainly manifested in enrichment of the ECM–receptor interaction and PI3K-Akt signaling pathways in females exposed to low alkalinity, and the GnRH secretion and chemokine signaling pathways in males. Glycosphingolipid biosynthesis and Ferroptosis pathway were enriched in females exposed to high alkalinity, and the Cortisol synthesis and secretion pathway were enriched in males. Overall, high-alkalinity exposure significantly delayed gonadal development in both sexes of largemouth bass, leading to reproductive impairment. Full article

In the current study, the impact of different acaricide treatments against Varroa (such as amitraz strips, oxalic and formic acid strips impregnated with glycerin, or the sublimation or instillation of oxalic acid) on glycerol residue levels of propolis was investigated. Propolis samples were collected from five beehives located in Chalkidiki (northern Greece), where all mentioned treatments were used and chemically analyzed and compared to control ones. Propolis samples were collected on days 7, 21, and 65 after the application of Varroa treatments, extracted with ethanol/water (70:30), silylated, and then analyzed using GC-MS, showing the predominance of diterpenes. The sublimation of oxalic acid and the amitraz treatment yielded a low glycerol residue (5.12% and 5.09% from 9.98% and 9.19%, respectively) in propolis specimens, while glycerin-impregnated oxalic acid strips led to elevated glycerol percentages (24.30% from 20.51%), unlike the reduced glycerol residues for all other treatments (instillation: 12.60% from 14.48% and glycerin-impregnated formic acid strips: 8.91% from 9.25%) and controls (3.27% from 6.30%). Furthermore, Principal Component Analysis (PCA) and the corresponding biplot illustrated how the sample composition varied across treatments and sampling days, highlighting the chemical constituent categories that most strongly contributed to these distinctions. These findings suggest that the use of glycerol-impregnated strips should be avoided in future beekeeping treatments against varroosis, as they could have a negative impact on the quality of propolis either for nutritional or medicinal applications. Full article

The coastal waters of Qinhuangdao are a major hotspot for harmful algal blooms (HABs) in the Bohai Sea, with Noctiluca scintillans being one of the primary algal species responsible for these events. A comprehensive understanding of the microbial community structure and functional responses to N. scintillans bloom events is crucial for elucidating their underlying mechanisms and ecological impacts. This study investigated the microbial community dynamics, metabolic shifts, and the environmental drivers associated with a N. scintillans bloom in the coastal waters of Qinhuangdao, China, using high-throughput sequencing of 16S and 18S rRNA genes, co-occurrence network analysis, and metabolic pathway prediction. The results revealed that the proliferation of autotrophic phytoplankton, such as Minutocellus spp., likely provided a nutritional foundation and favorable conditions for the N. scintillans bloom. The bloom significantly altered the community structures of prokaryotes and microeukaryotes, resulting in significantly lower α-diversity indices in the blooming region (BR) compared to the non-blooming region (NR). Co-occurrence network analyses demonstrated reduced network complexity and stability in the BR, with keystone taxa primarily belonging to Flavobacteriaceae and Rhodobacteraceae. Furthermore, the community structures of both prokaryotes and microeukaryotes correlated with multiple environmental factors, particularly elevated levels of NH₄⁺-N and PO₄³⁻-P. Metabolic predictions indicated enhanced anaerobic respiration, fatty acid degradation, and nitrogen assimilation pathways, suggesting microbial adaptation to bloom-induced localized hypoxia and high organic matter. Notably, ammonia assimilation was upregulated, likely as a detoxification strategy. Additionally, carbon flux was redirected through the methylmalonyl-CoA pathway and pyruvate-malate shuttle to compensate for partial TCA cycle downregulation, maintaining energy balance under oxygen-limited conditions. This study elucidates the interplay between N. scintillans blooms, microbial interactions, and functional adaptations, providing insights for HAB prediction and management in coastal ecosystems. Full article

Monitoring freshwater resources is essential for assessing the impacts of drought, water management and global warming. Spaceborne LiDAR altimeters allow researchers to obtain water height information, while water area and precipitation data can be obtained using different satellite systems. In our study, we examined 5 years (2018–2022) of data concerning the Euphrates–Tigris Basin (ETB), one of the most important freshwater resources of the Middle East, and the water bodies of both the ETB and the largest lake of Türkiye, Lake Van. A multi-sensor study aimed to detect and monitor water levels and water areas in the water scarcity basin. The ATL13 product of the Ice, Cloud, and Land Elevation Satellite-2 (ICESat-2) was used to determine water levels, while the normalized difference water index was applied to the Sentinel-2 optical imaging satellite to monitor the water area. Variations in both water level and area may be related to the time series of precipitation data from the ECMWF Reanalysis v5 (ERA5) product. In addition, our results were compared with global HydroWeb water level data. Consequently, it was observed that the water levels in the region decreased by 5–6 m in many reservoirs after 2019. It is noteworthy that there was a decrease of approximately 14 m in the water level and 684 km² in the water area between July 2019 and July 2022 in Lake Therthar. Full article

Atmospheric water harvesting, as an emerging water collection technology, is expected to mitigate water resource crises. Adsorption-based atmospheric water harvesting technology offers distinct advantages, including geographical independence and reduced reliance on ambient humidity levels. Herein, a thermoresponsive gel (PNIPAM/TO-CNF) integrated with lithium chloride was constructed to achieve accelerated moisture sorption and rapid desorption capabilities. In the designated PNIPAM/TO-CNF/LiCl gel, PNIPAM provided a temperature-responsive hydrophilic–hydrophobic transition network; the hydrophilicity and structural strength were enhanced by TO-CNF, the moisture absorption capacity was dramatically elevated by hygroscopic salt LiCl, and pore-forming agent polyethylene glycol created a favorable porous structure. This synergistic design endows the gel with an optimized hydrophilic network, temperature-responsive behavior, and a porous architecture conducive to water vapor transportation, thereby achieving rapid moisture absorption and desorption. Under 60% relative humidity, the gel exhibited a water vapor adsorption capacity of 144% within 1 h, reaching its maximum absorption capacity of 178% after 140 min. The gel exhibited an even more superior desorption performance: when heated to 70 °C, its moisture content rapidly decreased to 16% of its initial weight within 1 h, corresponding to the desorption of 91% of the total absorbed water. A simplified pore-forming methodology that enables the integration of temperature-responsive properties with efficient moisture transfer channels was reported in this paper, providing a viable design pathway for achieving accelerated adsorption–desorption cycles in atmospheric water harvesting. Full article

Background: Alzheimer’s Disease (AD) is one of the most prevalent neurodegenerative disorders and the most common form of dementia. Although current treatments examine disease progression, many have side effects and primarily target symptomatic relief as opposed to halting further neurodegeneration. Objective: The current study aims to determine the neuroprotective effects of water-soluble coenzyme Q10 (Ubisol-Q10) and an ethanolic Ashwagandha extract (E-ASH) on a transgenic mouse model of AD. Methods: A variety of immunofluorescence staining of biomarkers was conducted to assess mechanisms commonly implicated in the disease. Additionally, spatial and non-spatial memory tests evaluated cognitive functions at two timepoints throughout the progression of the disease. Results: A substantial reduction in microglial activation and amyloid-β (Aβ) plaques when treated with a combination of natural health products (NHPs), Ubisol-Q10 and E-ASH. Moreover, activation of autophagy was upregulated in both the Ubisol-Q10 and combination (Ubisol-Q10+E-ASH given as a combined “Tonic” solution) groups. Oxidative stress was decreased across treated groups, while astrocyte activation was elevated in both the E-ASH and Tonic group. The Tonic group expressed an elevation in the fluorescent intensity of neuronal nuclei (NeuN) and brain-derived neurotrophic factor (BDNF) levels. Interestingly, treatment with E-ASH and Ubisol-Q10 enhanced synaptic vesicle formation compared to controls. Pre-mortem memory tests revealed the treatments to be effective at preserving cognitive abilities. Conclusions: Based on these findings, the combination of E-ASH and Ubisol-Q10 may effectively mitigate the various mechanisms implicated in AD and ultimately prevent further disease progression. Full article

Coastal ecosystems have faced escalating environmental degradation in recent years, with eutrophication and nutrient imbalances emerging as critical concerns, particularly in estuarine regions. Understanding the spatiotemporal dynamics of key nutrients, including dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP), and silicate (SiO₃-Si), is essential for effective coastal management. This study examines the spatial and seasonal variations in these nutrients across 36 sampling sites in the Yellow River estuary from 2016 to 2018. Results indicate that DIN was the primary contributor to water quality degradation, with more than 27% of sampling sites exceeding the Class II seawater quality standard in 2018. Nutrient concentrations were notably elevated near the estuary. The eutrophication index (EI) revealed predominantly mild-to-moderate eutrophication levels throughout the study area. The study area exhibited a widespread phosphorus (P) limitation, with 44.4–94.4% of coastal waters experiencing P-restricted eutrophication. The N/P ratio significantly exceeded the Redfield ratio (16), indicating a pronounced nutrient imbalance. Furthermore, SiO₃-Si concentrations displayed a declining trend, highlighting the need for balanced nutrient management alongside eutrophication mitigation. Full article

For addressing the technical challenges of photon denoising and water level extraction in ICESat-2 satellite-based water monitoring applications, this paper proposes an innovative solution integrating Gaussian function fitting with Hough transform. The method first employs histogram Gaussian fitting to achieve coarse denoising of water body regions. Subsequently, a probability attenuation model based on elevation differences between adjacent photons is constructed to accomplish refined denoising through iterative optimization of adaptive thresholds. Building upon this foundation, the Hough transform technique from image processing is introduced into photon cloud processing, enabling robust water level extraction from ICESat-2 data. Through rasterization, discrete photon distributions are converted into image space, where straight lines conforming to the photon distribution are then mapped as intersection points of sinusoidal curves in Hough space. Leveraging the noise-resistant characteristics of the Hough space accumulator, the interference from residual noise photons is effectively eliminated, thereby achieving high-precision water level line extraction. Experiments were conducted across five typical water bodies (Qinghai Lake, Long Land, Ganquan Island, Qilian Yu Islands, and Miyun Reservoir). The results demonstrate that the proposed denoising method outperforms DBSCAN and OPTICS algorithms in terms of accuracy, precision, recall, F1-score, and computational efficiency. In water level estimation, the absolute error of the Hough transform-based line detection method remains below 2 cm, significantly surpassing the performance of mean value, median value, and RANSAC algorithms. This study provides a novel technical framework for effective global water level monitoring. Full article

This study delivers a pioneering, high-resolution hydrogeochemical assessment of surface waters in the Upper Velhas and Upper Paraopeba river basins within Brazil’s Iron Quadrangle—an area of critical socioeconomic importance marked by intensive mining and urbanization. Through a dense sampling network of 315 surface water points (one every 23 km²), the research generates an unprecedented spatial dataset, enabling the identification of contamination hotspots and the differentiation between lithogenic and anthropogenic sources of potentially toxic elements (PTEs). Statistical methods, including exploratory data analysis and cluster analysis, were applied to determine background and anomalous concentrations of potentially toxic elements (PTEs). Geospatial distribution maps were generated using GIS. The results revealed widespread contamination by As, Cd, Cr, Ni, Pb, and Zn, with many samples exceeding Brazilian, European, and global drinking water standards. Arsenic and cadmium anomalies in rural and peri-urban communities raise concerns due to the direct consumption of contaminated water. The innovative application of dense spatial sampling and integrated geostatistical methods offers new insights into the pathways and sources of PTE pollution, identifying specific lithological units (e.g., gold schists, mafic intrusions) and land uses (e.g., urban effluents, mining sites) associated with elevated contaminant levels. By establishing robust regional geochemical baselines and source attributions, this study sets a new standard for environmental monitoring in mining-impacted watersheds and provides a replicable framework for water governance, environmental licensing, and risk management in similar regions worldwide. Full article

This study examined the temporal dynamics of metal(loid) concentrations in agricultural soils, fern Amauropelta rivularioides, and surface waters in a peri-urban region on the Brazil–Paraguay border during 2019–2020. Elevated levels of As, Se, Co, Mn, Cu, and Zn raised concerns about environmental and human health risks, especially when compared to international guidelines. Post-harvest and pre-harvest periods, particularly during corn cultivation, revealed higher concentrations of toxic metals, suggesting cumulative effects of agrochemical use. Principal Component Analysis indicated significant geochemical variation, with particular emphasis on the Collection 1 period (1 June 2019). The fern A. rivularioides demonstrated metal accumulation, especially for As, Pb, Cr, and Ba, reflecting the influence of agrochemical residues and seasonal runoff. Surface waters displayed metal concentrations below detection limits, but phosphorus levels surpassed USEPA thresholds for eutrophication risk. Risk assessments indicated moderate to high contamination in soils, particularly for P, As, Mg, and Se. Hazard Quotient and Hazard Index values suggested chronic health risks, and Incremental Lifetime Cancer Risk values for dermal exposure to As, Pb, and Cr indicated an elevated cancer risk. Full article

This study investigated the effects of probiotic supplementation on performance, egg quality, antioxidant capacity, gut morphology, fecal microbiota, and bone morphology in Lohmann Brown laying hens aged 44 weeks over a 16-week period. Ninety-six hens were randomly divided into control and probiotic groups (n = 48 each). The probiotic group received probiotic supplement containing Lactobacillus acidophilus KUEN 1607 and Pediococcus acidilactici KUEN 1608 via drinking water at 0.5%. Probiotic supplementation significantly improved feed conversion ratio (FCR), eggshell strength and thickness, and albumen quality (p < 0.001) and reduced the incidence of cracked and shell-less eggs (p < 0.05). Yolk and serum cholesterol levels decreased (p < 0.001), and antioxidant parameters improved, along with elevated serum IgG (p < 0.001). Histological analysis showed an increased ratio of villus height to crypt depth (p < 0.001) in the jejunum, indicating enhanced intestinal health. Fecal samples revealed increased Lactobacillus spp. and reduced coliform counts (p < 0.001), suggesting improved gut microbiota balance. While bone volume and surface area showed no significant difference, 3D geometric morphometric analysis identified subtle shape changes in long bones, especially the femur and tibiotarsus. These findings demonstrate that the selected probiotic combination supports nutrient utilization, egg quality, gut integrity, immune status, and skeletal health, offering a sustainable strategy to enhance productivity and welfare in laying hens. Full article

Mining activities have long-term impacts on the environment even after the active stage. Historical mines developed in the 19th and 20th centuries for tin, copper, and mainly iron ore are located in the Pitkäranta area (Karelia, Russia). These objects are considered in our research as natural–anthropogenic sites of long-term water–rock interaction. Waters from flooded mines are the subject of this research. Redox conditions, pH, dissolved oxygen content, conductivity, and water temperature were determined during field work. The chemical composition of natural waters was determined by ICP-MS, ICP-AES, ion chromatography, potentiometric titration, and spectrophotometry. Our investigation showed that the mine waters are fresh and predominantly calcium–magnesium hydrocarbonate; most samples showed elevated sulfate ion contents. Circumneutral pH values and the absence of extremely high concentrations of heavy metals indicate neutral mine drainage. However the calculation of the accumulation coefficient showed the highest levels for siderophile elements relative to the corresponding data of the geochemical regional background. Moreover, zinc has the highest content in the series of heavy metal(loid)s considered. The maximum concentration of zinc was determined in the water of one of the shafts of the Lupikko mine, i.e., 5205 µg/L. The accumulation of heavy metals occurs in the process of long-term interaction of water–rock–organic matter under conductive redox conditions. Overall, the research highlighted the relevance of investigating the geochemistry of historical mines in the Pitkäranta area both from the perspective of environmental safety and the preservation of mining sites for scientific and educational purposes. Full article

The Gulf of Guinea is undergoing accelerated sea-level rise (SLR), with localized rates surpassing 10 mm yr⁻¹, more than double the global mean. Integrating GRACE/FO ocean mass data, reanalysis products, and machine learning, we identify a regime shift in the regional sea-level budget post-2015. Over 60% of observed SLR near major riverine outlets stems from ocean mass increase, driven primarily by intensified terrestrial hydrological discharge, marking a transition from steric to barystatic and manometric dominance. This shift coincides with enhanced monsoonal precipitation, wind-forced equatorial wave adjustments, and Atlantic–Pacific climate coupling. Piecewise regression reveals a significant 2015 breakpoint, with mean coastal SLR rates increasing from 2.93 ± 0.1 to 5.4 ± 0.25 mm yr⁻¹ between 1993 and 2014, and 2015 and 2023. GRACE data indicate extreme mass accumulation (>10 mm yr⁻¹) along the eastern Gulf coast, tied to elevated river discharge and estuarine retention. Dynamical analysis reveals the reorganization of wind field intensification, which modifies Rossby wave dispersion and amplifies zonal water mass convergence. Random forest modeling attributes 16% of extreme SLR variance to terrestrial runoff (comparable to wind stress at 19%), underscoring underestimated land–ocean interactions. Current climate models underrepresent manometric contributions by 20–45%, introducing critical projection biases for high-runoff regions. The societal implications are severe, with >400 km² of urban land in Lagos and Abidjan vulnerable to inundation by 2050. These findings reveal a hybrid steric–manometric regime in the Gulf of Guinea, challenging existing paradigms and suggesting analogous dynamics may operate across tropical margins. This calls for urgent model recalibration and tailored regional adaptation strategies. Full article