Search Results (2,381)

Background: Strychnos madagascariensis Poir (Loganiaceae) is a drought-tolerant indigenous fruit tree of East and southern Africa, valued for its food, medicinal, and socio-economic contributions to rural communities. Despite its importance as a candidate food crop, intraspecific morphological and genetic diversity had not previously been characterized, and no simple sequence repeat (SSR) markers had been developed for this species, leaving breeders and conservation planners without the basic diversity baseline needed to prioritize material for domestication. Methods: This study assessed vegetative and reproductive trait variation, variance components, and broad-sense heritability, and SSR-based genetic diversity among 27 morphologically defined S. madagascariensis morphotypes at Bonamanzi Game Reserve, KwaZulu-Natal, South Africa. Three trees were measured per morphotype (81 trees total), over two growing seasons. Genetic diversity was characterized in one representative tree per morphotype using seventeen newly developed SSR loci, the first such markers reported for this species, and analyzed with population structure (STRUCTURE version 2.3.4), PCA, and Nei’s genetic distance. Results: Twenty-seven morphotypes were identified based on leaf colour, shape, hairiness and size, dominated by grey (41%), elongated (59%), less hairy (48%), and medium-sized (>50–90 mm) leaves. Fruit diameter and mass showed the highest inter-morphotype variation (r = 0.949) and also the highest broad-sense heritability (H² = 55.3% and 47.8%, respectively), indicating strong genetic control of these traits and their suitability as targets for selective breeding. Environmental variance exceeded genotypic variance for most traits. A total of 144 alleles were identified across 17 SSR loci (mean 4.24 alleles/locus; mean PIC = 0.31). Population structure gave a preliminary, tentative signal of two genetic clusters (K = 2) with substantial admixture, which we interpret cautiously, given the limited sampling depth. Conclusions: This is the first study to characterize intraspecific morphological variation in S. madagascariensis and the first to develop SSR markers for the species. The results provide a preliminary, single-site framework for conservation genetics and crop improvement that should be validated with larger, multi-site samples. Grey morphotypes GyEvH1, GyEvH2, GyEvH3, GyRlH1 and GyEH2 combined consistent fruiting performance with favourable fruit-trait values and are proposed as priority candidates for further evaluation in domestication and breeding programmes. Full article

Insect larvae are naturally part of the diet of farmed animals, for instance poultry, pigs, and fish. Thus, the black soldier fly, Hermetia illucens (Linnaeus, 1758) (Diptera: Stratiomyidae) has been grown for use as a source of protein in animal feed. Black soldier fly larvae (BSFL) feed on various organic materials and bioaccumulate the nutrients obtained from the organic materials. This results in BSFL with protein content ranging from 25 to 60% depending on the type of organic material fed. Feeding strategies customized for optimizing BSFL growth and protein deposition are essential for sustainably increasing the production of BSFL to meet the growing demand for their use in animal feed. Feeding strategies for sustainable BSFL production should: ensure nutrient utilization efficiency to optimize BSFL growth and protein deposition; use readily available local organic material of good nutritional quality, safe, and acceptable for use in the animal feed industry; ensure economic and environmental sustainability; and adhere to existing legislature. While substantial information on feeding BSFL is available in different data sources, the literature mainly focuses on increasing BSFL production without integrating sustainability issues, especially economic and environmental sustainability. The objective of this review was to synthesize and consolidate existing information on feeding strategies for BSFL production from different sources and point out sustainable feeding strategies, existing knowledge gaps, and aspects that require further research. The purpose of the review is to provide information on feeding practices for the sustainable production of BSFL to meet the growing demand for BSFL in animal feed. This will contribute to improved food security, environmental management, and job creation. BSFL can feed on mixed organic material food sources more efficiently, reducing the volume of the food by up to 72%, while bioaccumulating the nutrients better than when feeding on individual organic sources such as fruit or vegetable waste. Full article

Microbial fuel cells (MFCs) are emerging as biomimetic bioelectrochemical systems that emulate naturally occurring microbial electron-transfer pathways for stimulus bioenergy generation and wastewater remediation. In this study, food–vegetable leachate (FVL) and sugarcane bagasse-derived biol were evaluated in combination with carbon fiber (CF) and biochar-modified carbon fiber (BCF) electrodes used as membrane components in MFCs. Four configurations, in duplicate, were constructed by coupling two substrates (biol or FVL) with two membrane types (CF and BCF). All systems exhibited progressive anodic acidification and up to a 55% increase in electrical conductivity. The highest voltage output was achieved in MFC-BL-2 (404.59 mV), followed by MFC-FL-1, driven by synergistic interactions between the substrate and biochar-enhanced conductive networks. MFC-FL-1 also demonstrated superior contaminant removal performance, achieving 60% COD reduction, 36% BOD reduction, and 50% NH₄⁺–N removal. SEM–EDS analysis confirmed that biochar-modified electrodes developed a porous structure and substantially enhanced microbial adhesion. FVL-fed systems formed dispersed electroactive biofilms that facilitated electron transfer, whereas biol-fed systems developed compact biofilms that constrained electron flux. By integrating waste-derived lignocellulosic materials with electroactive microbial consortia, this work advances a biomimetic circular bioengineering platform for sustainable bioelectrochemical recovery and wastewater remediation. Full article

Relict yew plants (Taxus L.) are not only ornamental plants with valuable wood but also have the ability to synthesize the unique compound taxol, which is successfully used in the treatment of cancer due to its powerful cytotoxic effect. Due to the presence of taxol, all parts of yew plants are extremely poisonous, but there have been cases where animals have eaten yew cones without fatal consequences. The biosynthesis of taxol is carried out due to the interaction of the isoprenoid and phenolic pathways of the secondary metabolism of plants. Despite the close attention of researchers to the peculiarities of taxol metabolism, there is very little data on the tissue and intracellular localization of both taxols and phenolic compounds in yew plants. Polyphenols are known to be physiologically active mediators involved in respiration, photosynthesis, plant growth and development, as well as in the process of in vitro dedifferentiation. Since Taxus is a relict species and has a limited and hard-to-reach range in nature, technologies that allow yew plants to be restored without removing plant material from the natural environment are of great practical importance: overcoming deep physiological dormancy of seeds, microclonal reproduction and initiation of plant growth. In vitro cultures are possible sources of biologically active and medicinal products. The aims and objectives of this study are to determine the characteristics of the formation and localization of phenolic compounds with high biological activity in various organs of plants of the genus Taxus and to determine the biological activity of ethanolic extracts from this plant. The objects of this study were the generative organs of Taxus canadensis, collected during the entire growing season (April–October) from plants growing in the Moscow region. The localization of various classes of polyphenols was determined by histochemical methods using light microscopy. Histochemical studies have shown the abundant presence of polyphenols in yew megastrobiles, microstrobiles, cones, seeds and aril. Ethanolic plant extracts were used to determine the biological activity. Flavans were dominant in the aril at various stages of vegetation, which was confirmed by our biochemical and histochemical studies. Extractive substances of T. canadensis show high antibacterial activity, especially in its shoot extracts. Ethanolic extracts from plant shoots showed greater biological activity than seed extracts. Aril extracts had the lowest cytotoxicity. Full article

The rapid expansion of urbanization in recent decades has intensified the urban heat island effect, driven by reduced vegetation cover, widespread use of heat-absorbing materials, and increases in surface and atmospheric temperature that may reach 5–6 °C. These conditions negatively impact well-being, quality of life, and human health. In response, numerous studies have examined mitigation strategies based on high-albedo materials and urban vegetation. This systematic review analyzes 225 peer-reviewed articles published between 2016 and 2025 addressing urban heat mitigation, surface thermal conditions, urban vegetation, outdoor thermal comfort and microclimate simulations. It provides a comprehensive synthesis, highlighting key findings and implications for future research. According to the Köppen–Geiger classification, most studies were conducted in humid subtropical and warm Mediterranean climates. The analysis focuses on urban canyon interventions, where vegetation is primarily modeled as shading trees (79.2%), along with other forms such as grass or shrubs (27.1%), mainly during the summer season. Results indicate that integrated mitigation strategies combining vegetation and high-albedo surfaces (≈0.8) generally provide greater cooling benefits than isolated interventions. Overall, the findings underscore the importance of the interaction between vegetation shading and surface properties for mitigating urban heat in outdoor spaces. Full article

Tomato (Solanum lycopersicum L.) is a globally important vegetable crop and a major dietary source of bioactive compounds, including lycopene, ascorbic acid, phenolics, and minerals. Modern tomato breeding has substantially improved yield, uniformity, and postharvest performance; however, these gains have often been accompanied by reduced flavor quality, lower nutritional value, and narrowing of the genetic base. This review synthesizes available evidence on Lithuanian tomato germplasm and evaluates its relevance for future breeding strategies aimed at enhancing genetic gain under Northern European conditions. The review integrates published data on genetic diversity, molecular characterization, morphological traits, fruit quality parameters, and yield performance of Lithuanian cultivars and hybrids developed in Lithuania. SSR-based studies indicate moderate genetic diversity, with mean expected heterozygosity of approximately 0.51 and mean PIC values of 0.47 in cultivars and 0.45 in hybrids, while also confirming a relatively narrow breeding pool. Lithuanian cultivars display substantial variation in fruit morphology, dry matter, soluble solids, firmness, lycopene, ascorbic acid, and yield. Traditional cultivars such as ‘Svara’, ‘Milžinai’, ‘Slapukai’, and ‘Balčiai’ show valuable nutritional and technological traits, whereas hybrids such as ‘Auksiai H’, ‘Adas H’, and ‘Ainiai H’ demonstrate improved productivity and firmness. The available evidence suggests persistent yield–quality trade-offs, particularly between productivity, soluble solids content, antioxidant accumulation, and postharvest performance. Although Lithuanian germplasm does not represent exceptionally broad genetic diversity, it contains regionally adapted material with stabilized trait combinations useful for breeding resilience, nutritional quality, and adaptation to temperate environments. Future progress will require broadening the genetic base and integrating traditional breeding with marker-assisted selection, genomic selection, GWAS, genome editing, multi-omics, and pangenomic approaches. Overall, Lithuanian tomato germplasm represents a locally adapted regional resource for translating genetic diversity into genetic gain in modern tomato breeding. Full article

Rapid urbanization in tropical Asia has led to a critical loss of green cover, exacerbating urban environmental challenges. While green roofs offer a promising Nature-based solution, their implementation in Asian countries is hindered by the prevalence of sloped roofs and high structural conversion costs. This research addresses this gap by developing a novel, lightweight vegetative roof tile designed as a direct structural replacement for conventional roofing materials in Sri Lanka. Existing roofing systems were studied, followed by a laboriousness study to determine the optimum tile dimensions. To meet these requirements, a modular tile measuring 900 mm × 1200 mm with a wave-shaped corrugated profile (a 10 mm rise and a 200 mm pitch) was engineered using SolidWorks 2024 and ABAQUS 2024 to meet Eurocode standards. Field investigations into plant health helped to finalize the depth of the roof tile as 2.5 cm. Following root penetration testing, fiber-reinforced plastic was selected for the tile structure to ensure durability while maintaining a total saturated weight of 52.5 kg/m². Biological testing demonstrated robust greening performance, with Axonopus compressus and Zoysia matrella achieving 100% survival rates and over 80% canopy coverage. This design methodology can be adapted across tropical Asia, contributing significantly to regional green infrastructure development and sustainable building practices. Full article

In rocky mountainous regions characterized by shallow, barren soils and water scarcity, non-rainfall water, such as condensation, plays a crucial ecological role in mitigating seasonal drought in forest trees. To enhance the water-use capacity of vegetation, this study utilized a previously developed eco-friendly PVA–CS/SA–Ca²⁺ hydrogel. The primary objective was to elucidate the synergistic mechanisms by which the hydrogel optimizes condensed water utilization and drives the ecophysiological recovery of Pinus tabuliformis and Platycladus orientalis, two keystone afforestation species in northern China. Utilizing a controlled environmental chamber to simulate the condensation and humidification process, the experiment established three treatments: a control group (CK), a pot-sealed group (PS, to isolate soil water absorption), and a hydrogel-amended group (Hydrogel-Root Wrapping, HRW). To comprehensively evaluate the water utilization mechanisms, the amount of condensed water captured by the system was quantified, and hydrogen isotope tracing techniques were employed to precisely track water transport pathways and contribution rates. Concurrently, key physiological parameters were systematically determined, including leaf water potential, stomatal conductance, leaf water content, net photosynthetic rate, and transpiration rate. The results demonstrated the following: (1) the hydrogel significantly enhanced the condensation water capture capacity of the system. The net mass gains of the Pinus tabuliformis and Platycladus orientalis systems under the HRW treatment reached 26.3 g and 32.9 g, respectively, which represented 1.17 and 1.30 times those of the CK treatment, and 1.52 and 1.54 times those of the PS treatment. (2) Isotope tracing confirmed that both tree species possess significant Foliar Water Uptake (FWU) capacity. Following condensation, the δ²H values in the leaves of Platycladus orientalis and Pinus tabuliformis surged to 113.5‰ and 85.3‰, respectively, with stem δ²H values increasing by 31‰ and 22‰ compared to their initial baseline. (3) The introduction of the hydrogel in the HRW treatment provided 11.2% and 10.9% of the stem water supply for Platycladus orientalis and Pinus tabuliformis, respectively, thereby reducing their dependence on soil water by 8.3% and 13.1%. In contrast, there was no significant difference in the fractional contribution of condensation water to stem water between the PS and CK treatments. (4) Regarding physiological responses, the application of the hydrogel material effectively improved the physiological status of the plants. The leaf water potentials of Pinus tabuliformis and Platycladus orientalis increased to −0.15 MPa and −1.32 MPa, respectively. Concurrently, stomatal conductance (3.25 and 3.64 mm·s⁻¹) and leaf water content (58.4% and 67.4%) were significantly higher than those in the other treatments. In summary, the hydrogel can significantly enhance the capture, conversion, and utilization efficiency of condensation water by vegetation, effectively optimizing the water supply dynamics of the system. This provides key theoretical and technical support for ecological afforestation in difficult sites within rocky mountainous areas. Full article

The climate crisis, housing precarity, and the loss of everyday architectural heritage are converging challenges in Mediterranean cities. This article investigates the adaptive reuse of early twentieth-century adobe refugee dwellings in Nea Ionia and Kaisariani, neighborhoods of Attica, Greece, as an integrated social, environmental, and cultural strategy. Historical documentation, urban-morphological analysis, field observations, building survey data, material assessment, and design-based microclimatic analysis were combined to evaluate compatible restoration and bioclimatic upgrades as alternatives to demolition and conventional energy retrofit practices, with the main aim of preserving an important part of Greek history and architecture. The study develops a replicable qualitative assessment framework that identifies how existing adobe envelopes, compact layouts, courtyards, thresholds, vegetated pergolas, and low-water evaporative cooling may support low-carbon housing reuse. The results clarify the current preservation conditions and reuse potential of the selected case-study fragments, showing that adobe dwellings can preserve embodied material value, retain thermal mass and hygroscopic regulation, and support social housing when repaired with compatible, low-impact techniques. The article argues that the reuse of adobe refugee dwellings can function as a distributed urban strategy for housing provision, heritage continuity, and microclimatic adaptation. Its main contribution is a transferable analytical framework for assessing overlooked earthen housing stocks in dense Mediterranean contexts. The study argues that adaptive reuse can serve simultaneously as a means of social housing, a mechanism for optimizing the microclimate, and a means of preserving the tangible and intangible heritage of Greek adobe buildings that have been standing for over 100 years. This position extends circular construction debates by prioritizing non-demolition and direct reuse while preserving an important period of history. Full article

Although the green belt around Greater Lomé (GBGL) is a vital ecological buffer, it is currently facing significant degradation. This decline appears to be associated with a combination of various socioeconomic uses by the local community and formal operations of established businesses. Grounded in the cultural materialism framework, this study aims to contribute to a better understanding of the dynamics of the socioeconomic uses of the green belt around Greater Lomé in a context of degradation and investigates the dynamics of these socioeconomic uses and their environmental impacts through a multidisciplinary methodology. This approach combines anthropological analysis based on field observation, 53 semi-structured interviews and 5 focus groups, a quantitative questionnaire survey (n = 384) and an analysis of land use and land cover (LULC) dynamics derived from Landsat imagery (2003–2023). The results reveal six main types of socioeconomic uses of the GBGL (notably land transactions, agriculture, breeding and grazing, exploitation of wood energy, timber and utility wood, sand mining, and waste disposal), which lead to complex social dynamics ranging from conflicts to alliances among stakeholders. The LULC dynamics analysis indicates a staggering 468.26% expansion in built-up areas over the last 20 years, at the expense of swamp vegetation/gallery forest (−76.79%), tree-and-shrub savanna (−53.47%) and plantations (−49.43). This study provides a scientific basis supporting the urgent necessity to establish the GBGL as a legally protected entity and argues in favour of an inclusive management model that is designed to reconcile the socioeconomic survival needs of local populations with sustainable preservation of essential ecosystem services. Full article

Radar is critical for urban security against Unmanned Aerial Vehicles (UAVs), yet signal occlusion caused by dense buildings and complex urban structures remains a major challenge for coverage assessment. Existing approaches commonly rely on 2D maps or 2.5D Digital Surface Models (DSMs), which have difficulty representing vertical facades, vegetation, bridges, overhanging structures, and void spaces. These geometric limitations can introduce errors in radar occlusion determination and direct-path power-density estimation. Full 3D ray-tracing methods offer high fidelity, but their multi-path modeling and material-parameter requirements can be costly for large oblique photogrammetric city meshes. To address this problem, this paper proposes the Visible Radar Power-Density Field (VRPF), a 3D radar power-density field computation framework based on high-resolution oblique photogrammetric models. The method constructs a reusable spatial index for large numbers of triangular facets and performs two-stage occlusion queries: rapid Axis-Aligned Bounding Box (AABB) pruning followed by ray-triangle intersection tests. Together, these components enable efficient direct-path power-density calculation while accounting for line-of-sight occlusion in complex urban scenes. Qualitative and quantitative experiments show that VRPF better preserves occlusion boundaries around building edges, vegetation, and elevated structures than DSM-based baselines. VRPF also requires less time for repeated occlusion queries than a conventional 3D BVH ray-casting baseline while maintaining highly consistent radar-signal occlusion determinations. With 32 threads, VRPF computes power density for ${10}^8$ target points in 5.92 s, about $2.66\times$ faster than the 1 m DSM method. These results indicate that VRPF provides a practical balance between geometric fidelity and computational efficiency for direct-path radar power-density assessment with urban geometric occlusion. Full article

Pumpkin (Cucurbita moschata Duchesne) is an economically important vegetable crop in the family Cucurbitaceae. However, existing genetic transformation systems for pumpkin are limited by cultivar dependence, low efficiency, lengthy regeneration, and poor stability. In this study, we developed and optimized an Agrobacterium rhizogenes-mediated hairy root induction system for pumpkin leaves under non-tissue culture conditions, using the cultivar ‘Jintian No. 1’ as the experimental material. Systematic comparisons were performed across multiple parameters, including infection method, duration, bacterial strain, bacterial concentration, seedling age, and leaf position. The optimized transformation conditions were as follows: the second fully expanded leaf of 30-day-old seedlings, A. rhizogenes strain K599 at an optical density (OD₆₀₀) of 0.8, and direct immersion for 15 min. Under these optimized conditions, the positive hairy root induction rate reached 89.58%. Moreover, the system successfully induced positive hairy roots in all tested pumpkin cultivars. In summary, this study establishes a highly efficient and practical hairy root induction system for pumpkin leaves, providing a robust platform for root-specific gene functional analysis and rapid validation of candidate genes related to root development and stress responses in pumpkin. Full article

Growing demand for natural and functional foods has increased interest in fermentation-based technologies. This study aimed to develop and characterize fermented and freeze-dried vegetable snacks from beetroot, potato, zucchini, and cucumber. The evaluation included physicochemical properties (pH, titratable acidity, and color), microbiological quality, antioxidant potential using TEAC, ABTS and FRAP assays, along with determination of total phenolic content (TPC), vitamin C, sensory acceptability and attributes. Fermentation resulted in a significant decrease in pH and an increase in titratable acidity of samples, confirming active growth of lactic acid bacteria (LAB), with counts exceeding 6 log₁₀ CFU/g after five days. Freeze-drying preserved the acidic profile and influenced color parameters. Antioxidant capacity varied depending on the raw material, with beetroot showing the strongest potential to scavenge the ABTS^•+, while cucumber was characterized by the highest vitamin C content (175.24 mg/100 g dry matter. Zucchini exhibited moderate antioxidant capacity, whereas potato showed the lowest values. Sensory analysis indicated high acceptance of beetroot and potato snacks, associated with balanced flavor and texture, while cucumber and zucchini samples were rated lower due to softer texture and higher acidity. The combination of lactic fermentation and freeze-drying enables the production of vegetable snacks with desirable sensory properties and enhanced functional potential. Full article

Apple (Malus domestica Borkh.) rootstocks play a key role in modern intensive orchard systems, where their accurate identification is essential for breeding, nursery production, and certification of planting material. This is particularly important in Kazakhstan, a recognized center of origin of cultivated apple, where local germplasm remains insufficiently characterized at the molecular level. In this study, we integrated simple sequence repeat (SSR) genotyping and morphological trait analysis to develop a reliable approach for the identification of clonal apple rootstocks cultivated in Kazakhstan. Five widely used rootstocks (Zhetysu 5, ARM-18, B-7-35, M9, and B9) were analyzed using 17 polymorphic SSR markers and 30 vegetative traits. SSR analysis revealed moderate genetic polymorphism (PIC = 0.28–0.54; He = 0.35–0.58) and enabled clear discrimination among all studied genotypes. Cluster analysis based on genetic distances grouped rootstocks according to their genetic similarity, reflecting their origin and differentiation. Morphological evaluation demonstrated significant phenotypic variability and identified correlations among key vegetative traits related to plant vigor and leaf development. The integration of molecular and morphological data allowed the development of comprehensive genotype profiles (“molecular–morphological passports”) for each rootstock, ensuring their reliable identification. The proposed approach provides a practical framework for the certification of planting material and the management of apple genetic resources in Kazakhstan. It can be applied to improve nursery systems, support breeding programs, and ensure the production of true-to-type planting material in modern horticulture. Full article

Enormous genetic diversity exists in rice germplasm, including wild and weedy relatives, though they remain unexplored within in situ or ex situ collections. Characterisation and utilisation of the available biodiversity in plant breeding is essential for the detection of novel traits or genes for climate resilience. In this study, 97 rice genotypes, including 90 rice accessions belonging to various Oryza species and 7 check cultivars with an O. sativa background, were characterised for quantitative morphological characters following the guidelines based on distinctiveness, uniformity and stability (DUS) test by the Protection of Plant Varieties and Farmers’ Rights Authority (PPVFRA), India. Characterisation of the genotypes based on 39 important DUS morphological descriptors revealed polymorphism in 35 traits, confirming high morphological diversity among wild rice accessions and distinguishing and unique traits from other wild accessions for the utilisation in pre-breeding programmes. Genotypes such as WD5_6, WD10_4, and WD3_3 consistently expressed a favourable combination of broad and long leaves, extended panicle length, and well-branched panicles with higher panicle number. In addition, these genotypes showed purple pigmentation across multiple vegetative and reproductive organs, indicating stable and enhanced anthocyanin accumulation. Accessions WD10_4 and WD3_3 also represent valuable donors for panicle architecture and yield component enhancement, while genotypes such as WD17_15 and WD12_8 may serve as specific donors for panicle length and branching traits. Characterisation studies and detection of unique traits provide the empirical foundation for conservation decisions, taxonomic clarity, and pre-breeding applications. Interspecific crosses in the genetic background of elite cultivars with donor species viz., O. barthii, O. glaberrima and O. rufipogon were developed as pre-breeding materials for further crop improvement as well as for the identification of novel genes of agronomic importance. Full article