Search Results (3,654)

Accurate parametric modeling of lithium-ion batteries is essential for battery management system (BMS) design in electric vehicles and broader energy storage applications, enabling reliable state estimation and effective thermal control under diverse operating conditions. This study presents a detailed characterization of lithium-ion cells to support advanced BMS in electric vehicles and stationary storage. A second-order equivalent circuit model is developed to capture instantaneous and dynamic voltage behavior, with parameters extracted through Hybrid Pulse Power Characterization over a broad range of temperatures (−10 °C to 45 °C) and state-of-charge levels. The method includes multi-duration pulse testing and separates ohmic and transient responses using two resistor–capacitor branches, with parameters tied to physical processes like charge transfer and diffusion. A weakly coupled electro-thermal model is presented to support real-time BMS applications, enabling accurate voltage, temperature, and heat generation prediction. This study also evaluates open-circuit voltage and direct current internal resistance across pulse durations, leading to power capability maps (“fish charts”) that capture discharge and regenerative performance across SOC and temperature. The analysis highlights performance asymmetries between charging and discharging and confirms model accuracy through curve fitting across test conditions. These contributions enhance model realism, thermal control, and power estimation for real-world lithium-ion battery applications. Full article

Mitochondrial fission and fusion appear to be relatively infrequent in cardiac cells compared to other cell types; however, the proteins involved in these events are highly expressed in adult cardiomyocytes (ACM). Therefore, these proteins likely have additional non-canonical roles. We have previously shown that DRP1 not only participates in mitochondrial fission processes but also regulates mitochondrial bioenergetics in cardiac tissue. However, it is still unknown where the DRP1 that does not participate in mitochondrial fission is located and what its role is at those non-fission spots. Therefore, this manuscript will clarify whether oligomeric DRP1 is located at the SR–mitochondria interface, a specific region that harbors the Ca²⁺ microdomains created by Ca²⁺ release from the SR through the RyR2. The high Ca²⁺ microdomains and the subsequent Ca²⁺ uptake by mitochondria through the mitochondrial Ca²⁺ uniporter complex (MCUC) are essential to regulate mitochondrial bioenergetics during excitation–contraction (EC) coupling. Herein, we aimed to test the hypothesis that mitochondria-bound DRP1 preferentially accumulates at the mitochondria–SR contacts to deploy its function on regulating mitochondrial bioenergetics and that this strategic position is modulated by calcium in a beat-to-beat manner. In addition, the mechanism responsible for such a biased distribution and its functional implications was investigated. High-resolution imaging approaches, cell fractionation, Western blot, 2D blue native gel electrophoresis, and immunoprecipitations were applied to both electrically paced ACM and Langendorff-perfused beating hearts to elucidate the mechanisms of the strategic DRP1 localization. Our data show that in ACM, mitochondria-bound DRP1 clusters in high molecular weight protein complexes at mitochondria-associated membrane (MAM). This clustering requires DRP1 interaction with β-ACTIN and is fortified by EC coupling-mediated Ca²⁺ transients. In ACM, DRP1 is anchored at the mitochondria–SR contacts through interactions with β-ACTIN and Ca²⁺ transients, playing a fundamental role in regulating mitochondrial physiology. Full article

Giant somatosensory evoked potentials (gSEPs) are abnormally high-amplitude cortical responses to peripheral nerve stimulation, traditionally regarded as electrophysiological hallmarks of progressive myoclonic epilepsies (PMEs). However, accumulating evidence shows their presence in a broader range of non-epileptic conditions, including focal lesions, metabolic encephalopathies, neurodegenerative diseases, and even functional disorders. This review offers a comprehensive analysis of the physiological mechanisms, diagnostic criteria, and clinical significance of gSEPs, integrating data from both classical and emerging neurophysiological techniques. gSEPs are mainly produced in the primary somatosensory cortex through mechanisms involving cortical disinhibition, impaired GABAergic transmission, and altered thalamocortical connectivity. In epileptic syndromes such as Unverricht–Lundborg disease and other PMEs, gSEPs reflect cortical hyperexcitability and are closely linked to cortical myoclonus. Conversely, in non-epileptic contexts, they may indicate transient or chronic cortical dysfunction. The diagnostic utility of gSEPs ranges from differential diagnosis of myoclonus to monitoring disease. However, heterogeneity in amplitude definitions and recording protocols hinders the standardization of these measurements. This may result in the identification of the right threshold to differentiate conditions associated with simple increased versus giant SEP, the latter of which may help identify truly epileptic conditions from other disorders simply associated with increased SEP amplitude. Full article

An attempt was made to understand the interactive consequences of subjecting a rhizospheric microbial community of xTriticocereale (Triticale) to higher CO₂ levels and soil nitrogen addition in the short term in a tropical agro-ecosystem. Open-top chambers (OTCs) were used to grow the test crops for a single season under ambient CO₂ (AC) and elevated CO₂ (EC) along with two variable N dosages: recommended (N₀: 0.053 g N/kg of soil) and high (N₂: 0.107 g of N/kg of soil) levels. Variations in the composition of microbial communities and abundances were investigated using phospholipid fatty acid analysis (PLFA). A significantly (p < 0.001) increased microbial biomass content (MB) was observed under EC compared to AC, while the addition of N had a minor effect. A decreased fungi/bacteria (F/B) ratio (~38%) was observed with high N application in the CO₂ enrichment treatment. Bacteria were more abundant, while fungal abundance decreased under N₂ and EC. Gram (+ve) bacteria used these conditions to thrive under N₂ and EC, while Gram (−ve) bacteria declined. No significant effects on actinomycetes were noticed in any of the treatments. However, eukaryotes acquired more benefits and flourished in response to EC. Varied responses were noted for the Shannon diversity index (H’) under EC. Overall, (i) bacteria (Gram-positive) and eukaryotes dominated under EC and high N addition, while fungi decreased, and (ii) EC and high levels of N addition did not affect actinomycetes. Short-term exposure under the given conditions was found to alter the rhizospheric microbial community. However, multiple season studies are needed to elucidate whether these short-term responses are transient or continuous. Full article

A significant application of the proportional–integral (PI) controller in the automotive sector is in electric motors, particularly brushless direct current (BLDC) motors utilized in electric vehicles (EVs). This paper presents a high-performance boost converter regulated by a fractional-order proportional–integral (FoPI) controller to ensure stable output voltage and power delivery to effectively charge the battery under fluctuating input conditions. The FoPI controller parameters, including gains and fractional order, are optimized using an Arithmetic Harris Hawks Optimization (AHHO) algorithm with an integral absolute error (IAE) as the objective function. The primary objective is to enhance the system’s robustness against input voltage fluctuation while charging from renewable sources. Conversely, regenerative braking is crucial for energy recovery during vehicle operation. This study implements a fractional-order PI controller (FOPI) for the smooth and exact regulation of speed and energy recuperation during regenerative braking. The proposed scheme underwent extensive simulations in the Simulink environment using the FOMCON toolbox version 2023b. The results were validated with the traditional Ziegler–Nichols method. The simulation findings demonstrate smooth and precise speed control and effective energy recovery during regenerative braking and a constant voltage output of 375 V, with fewer ripples and rapid transient responses during charging of batteries from variable input supply. Full article

Cutaneous malignant melanoma remains one of the most aggressive forms of skin cancer, characterized by high metastatic potential and resistance to standard therapies. Emerging evidence suggests that transient receptor potential (TRP) channels, non-selective cation channels involved in calcium homeostasis, and cellular stress responses play a pivotal role in melanoma development and progression. This review highlights the physiological expression of key TRP subfamilies (TRPM1, TRPM7, TRPM8, TRPV1, TRPV4, and TRPM2) in melanocytes and discusses their dysregulation in melanoma cells. TRPM1 is implicated as a tumor suppressor, whereas TRPM7, TRPV1, and TRPV4 often function as both melanoma suppressor or oncogenic drivers, modulating proliferation, apoptosis, and metastasis. TRPM2, which is responsive to oxidative stress, supports melanoma cell survival under metabolic stress. The potential of TRP channels as diagnostic biomarkers and therapeutic targets is evaluated, with attention paid to current pharmacological approaches and research challenges. The complexity and context-dependency of TRP function in melanoma underscore the need for isoform-specific modulation and personalized therapeutic strategies. Full article

This study investigates the potential of transient potentiometric signals generated by an array of ion-selective electrodes (ISEs) as the basis for a potentiometric electronic tongue capable of identifying and quantifying a range of inorganic and organic cations. Six distinct polymeric membrane ISEs were fabricated, differing in plasticizer type (either NPOE or DEHS), and in the presence or absence of a lipophilic ion exchanger (KTClPB) and/or an ionophore (DB18C6). Transient potential responses were recorded following the exposure of the electrode array to various cations at different concentrations. A total of 810 transient signals were analyzed through visual inspection and principal component analysis (PCA), revealing characteristic dynamic patterns influenced by membrane composition, ion type, and ion concentration. PCA was conducted both on the transient signals from each individual electrode and on the aggregated dataset comprising signals from the full six-electrode array (electronic tongue). The electronic tongue exhibited a markedly enhanced capacity for discriminating and quantifying ion concentrations in comparison to any single electrode. Full article

Rapid pressure fluctuations—known as hydraulic transients—occur during valve operations or load changes in turbines and pumps. The presence of biofouling, particularly caused by the golden mussel (Limnoperna fortunei), can intensify these effects and compromise the structural integrity of pressurized systems. This study experimentally evaluated the influence of such biofouling on pressure peaks during transient events in forced conduits. A hydraulic test rig was developed using PVC pipes with nominal diameters of 2½”, 3”, and 4”, tested under both clean conditions and with simulated biofouling printed in 3D, replicating mussel morphology. Results showed that, under the same initial flow rates, pressure peaks in biofouled pipes were significantly higher than in clean ones, especially in smaller diameters. To mitigate structural risks, the downstream shut-off valve closure time was modulated using a needle valve, effectively reducing peak pressures to levels closer to design limits. It is concluded that L. fortunei colonization alters transient hydraulic behavior and should be considered in the design and operation of systems vulnerable to biofouling, particularly in critical infrastructure such as water supply networks and hydroelectric power plants. Full article

Background: Hepatocellular carcinoma (HCC) is the most common primary liver cancer and remains a significant global health challenge. Transarterial chemoembolization (TACE) is the treatment of choice for intermediate-stage HCC patients. While TACE induces localized cytotoxic and ischemic tumor necrosis, the resultant hypoxia paradoxically activates pro-angiogenic signaling pathways, which may promote tumor revascularization and recurrence. This study aimed to evaluate the plasma levels of angiogenetic factors pre- and post-TACE to assess their dynamic changes and potential clinical implications. Methods: Twenty-five intermediate-stage HCC patients were included in this monocentric prospective study. Peripheral blood samples were collected at baseline (pre-TACE), 24 h, 3 days, and 1 month post-TACE. Angiogenic factor levels were analyzed using a multiplex bead-based assay. Results: Angiopoietin-2 levels were significantly elevated three days post-TACE, followed by a gradual decline after one month. A similar pattern was observed for hepatocyte growth factor, with a marked increase at 24 h post-TACE and subsequent normalization. Endothelin-1 also exhibited a temporary increase, although it was only detected in four patients. Fibroblast growth factors (1 and 2) and vascular endothelial growth factor A were detected in a limited number of patients, which may indicate low systemic release or the need for a more sensitive detection method. Conclusions: These findings suggest that TACE induces a transient increase in angiogenic factors, likely due to tumor ischemia, tissue injury, or microenvironmental responses. Future studies should explore more sensitive detection methods and evaluate whether these factors could serve as prognostic biomarkers or therapeutic targets in HCC treatment. Full article

This paper presents a calibrated and dynamically responsive simulation framework for hybrid energy systems that integrate Proton Exchange Membrane Fuel Cells (PEMFCs) and batteries, targeting applications in light commercial vehicles (LCVs). The aim is to support the design and assessment of energy management strategies (EMS) under realistic operating conditions. A publicly available PEMFC model is used as the starting point. To improve its representativeness, calibration is performed using experimental polarization curve data, enhancing the accuracy of the stack voltage model, and the air compressor model—critical for maintaining stable fuel cell operation—is adjusted to reflect measured transient responses, ensuring realistic system behavior under varying load demands. Quantitatively, the calibration results are strong: the R² values of both the fuel cell polarization curve and the overall system efficiency are around 0.99, indicating excellent agreement with experimental data. The calibrated model is embedded within a complete hybrid vehicle powertrain simulation, incorporating longitudinal dynamics and control strategies for power distribution between the battery and fuel cells. Simulations conducted under WLTP driving cycles confirm the model’s ability to replicate key behaviors of PEMFC-battery hybrid systems, particularly with respect to dynamic energy flow and system response. In conclusion, this work provides a reliable and high-fidelity simulation environment based on empirical calibration of key subsystems, which is well suited for the development and evaluation of advanced EMS algorithms. Full article

Magnetic gears are becoming promising devices that can replace conventional mechanical gears in several applications, where reduced maintenance, absence of lubrication and intrinsic overload protection are especially relevant. This paper focuses on the experimental analysis of a coaxial magnetic gear prototype recently developed at the Department of Industrial Engineering of the University of Padova. It is found that its efficiency is high and aligned with prototypes in the literature, its stationary response confirms the velocity ratio of the corresponding mechanical planetary gear, the overload protection is aligned with numerical prediction, while the dynamic response highlights that the intrinsic compliance of the magnetic coupling prevents the use of such device in high-frequency transients. It is concluded that the proposed architecture can be effectively employed for speed reducers applications where low-frequency modulation is sufficient, which includes many industrial applications. Nevertheless, high rotational speeds are allowed. The performance characteristics, although specific for the prototype considered, experimentally highlights the key features of coaxial magnetic gear devices. The experimental performance are also compared with estimations from the literature, when available. Full article

This paper investigates the stabilization problem of a class of nonlinear systems characterized by non-minimum phase behavior within each subsystem, with a focus on an application to a gantry crane system that employs friction to control its swing angle. In practical crane operations, the demand for accelerated system response is critical to improving productivity; however, this often induces significant variations in the swing angle, potentially destabilizing the system. To overcome this challenge, we propose a hybrid control approach that combines the concept of multi-diffeomorphism with symmetry considerations to enhance the smoothness of transient responses. Unlike classical input–output feedback linearization, which typically relies on a single diffeomorphism and may compromise the zero dynamics stability, the proposed method distributes the transformation across multiple diffeomorphisms, ensuring balanced and coordinated transient behavior. The design involves the simultaneous development of subsystem-dependent feedback controllers, which collaboratively guarantee the global stability of the overall closed-loop nonlinear gantry crane system. The Lyapunov stability framework is employed to rigorously demonstrate that the tracking errors converge asymptotically to meet the desired performance specifications. In addition, the simulation results demonstrate that the developed hybrid control approach notably enhances the system’s responsiveness while preserving both symmetry and the stability of the zero dynamics. Specifically, the swing angle decreases by over 90% in less than 2 s, highlighting the method’s efficiency in minimizing oscillations during fast operations. This study highlights the practical benefits of integrating symmetry-aware multi-diffeomorphism techniques into nonlinear control design. Such techniques are found to be particularly effective for underactuated mechanical systems like gantry cranes. Full article

In response to the growing demand for lightweight and high-efficiency industrial equipment, this study addresses the critical issue of lubrication failure in high-speed, heavy-duty gear reducers, which often leads to reduced transmission efficiency and premature mechanical damage. A three-dimensional transient multiphysics-coupled model of oil-jet lubrication is developed based on computational fluid dynamics (CFD). The model integrates the Volume of Fluid (VOF) multiphase flow method with the shear stress transport (SST) k−ω turbulence model. This framework enables the accurate capture of oil-jet interface fragmentation, reattachment, and turbulence-coupled behavior within the gear meshing region. A parametric study is conducted on oil injection velocities ranging from 20 to 50 m/s to elucidate the coupling mechanisms between geometric configuration and flow dynamics, as well as their impacts on oil film evolution, energy dissipation, and thermal management. The results reveal that the proposed method can reveal the dynamic evolution characteristics of the gear injection lubrication. Adopting an appropriately moderate injection velocity (30 m/s) improves oil film coverage and continuity, with the lubricant transitioning from discrete droplets to a dense wedge-shaped film within the meshing zone. Optimal lubrication performance is achieved at this velocity, where oil shear-carrying capacity and kinetic energy utilization efficiency are maximized, while excessive turbulent kinetic energy dissipation is effectively suppressed. Dynamic monitoring data at point P further corroborate that a well-tuned injection velocity stabilizes lubricant-velocity fluctuations and improves lubricant oil distribution, thereby promoting consistent oil film formation and more efficient heat transfer. The proposed closed-loop collaborative framework—comprising model initialization, numerical solution, and post-processing—together with the introduced quantitative evaluation metrics, provides a solid theoretical foundation and engineering reference for structural optimization, energy control, and thermal reliability design of gearbox lubrication systems. This work offers important insights into precision lubrication of high-speed transmissions and contributes to the sustainable, green development of industrial machinery. Full article

This paper presents the design and implementation of a bidirectional resonant converter with enhanced dynamic response to electric vehicles (EV). The proposed system comprises an assembly of four switches, a capacitor, and an inductor on both the primary and secondary sides of the transformer. The value of C-L-L-C was calculated using the first harmonic approximation method. Moreover, the small-signal analysis method was used to design the control system and analyze the dynamic performance of the system. Closed-loop control algorithms for voltage and current loops with synchronous rectifiers (SRs) were designed and implemented on a 32-bit microcontroller (STM32G474RET6). A 70 kHz, 400 W prototype is built with a peak conversion efficiency of 95.05% using SR in the forward mode. Without SR, the peak conversion efficiency was 93.57% in the forward mode and 93.04% in the reverse mode. In the forward mode, the proposed algorithm reduced the settling time to 15 ms, in contrast to the 40 ms associated with the conventional algorithm; in the reverse mode, the proposed algorithm reduced the settling time to 10 ms, in contrast to the 15 ms associated with the conventional algorithm. Full article

This paper proposes a cost-effective five-component discrete capacitive pre-charging circuit designed to mitigate charge redistribution effects in Analog-to-Digital Converter (ADC) inputs, particularly for low-cost embedded applications involving multiplexed high-output-resistance sources. The paper presents an analysis and experimental validation of this approach, comparing its performance against traditional methods like grounding or leaving unused multiplexer inputs floating. The proposed solution leverages external components (two capacitors and three switches) and multiplexer features to pre-charge ADC inputs to approximately half the reference voltage, which could be taken directly from the multiplexer supply rail, significantly reducing transient glitches and settling times. The experimental results demonstrate a clear improvement, achieving settling times up to 1.4 µs shorter than conventional approaches during specific multiplexer transitions. Component selection guidelines are outlined, including compensation capacitor sizing and transistor choice, addressing practical concerns such as charge injection effects. Despite certain experimental constraints noted during testing, the developed discrete pre-charging method consistently exhibited substantial performance gains. Our findings confirm that this practical, minimal-component strategy effectively addresses charge redistribution challenges, presenting an efficient solution for enhancing ADC input accuracy and response speed in resource-limited embedded sensor systems. Full article