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21 pages, 452 KiB  
Article
Heat-Loss Based Method for Real-Time Monitoring Method for Hydroelectric Power Plant Efficiency
by Lorenzo Battisti, Lorenzo Tieghi and Soheil Fattahi
Energies 2025, 18(10), 2586; https://doi.org/10.3390/en18102586 - 16 May 2025
Viewed by 430
Abstract
In energy transition scenarios, hydropower remains the largest source of renewable electricity generation. However, with respect to other means of renewable energy exploitation, like wind turbines or photovoltaics, very few technological advancements are to be expected, due to the technological maturity of hydropower [...] Read more.
In energy transition scenarios, hydropower remains the largest source of renewable electricity generation. However, with respect to other means of renewable energy exploitation, like wind turbines or photovoltaics, very few technological advancements are to be expected, due to the technological maturity of hydropower turbines. Therefore, an increase in power production of hydropower plants can only be possible thanks to an optimization of the operation and maintenance policies, leading to improved performance, reducing energy losses and downtimes. This work proposes a practical approach to the continuous monitoring of the operational conditions of hydropower plants through the non-invasive measurement of the electrical efficiency of the generator group. To achieve this, a heat-loss based method is introduced, which enables the measurement of both the electrical generator losses and the electrical input power, along with their associated uncertainties. This method is applicable for plants of any size, does not require a production shutdown, and, since it is applied to the electrical generator, can be used with different turbine types, including Kaplan, Francis, and Pelton. It also relies on relatively simple instruments such as thermo-cameras, thermo-resistances, thermo-couples, and flow meters to measure key variables, including cooling water inlet and outlet temperatures, electrical machine external and frame temperatures, undisturbed ambient temperature, electrical power absorbed, and cooling water flow rate. The proposed methodology has been tested and validated through the application to a laboratory test rig. In all test conditions, the heat loss-based method showed a smaller relative error than the standard efficiency measurement methods. Full article
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11 pages, 2652 KiB  
Article
Thermal Resistance Enhancement and Wettability Amelioration of Poly(benzimidazole-aramid) Film by Silica Nanocomposites
by Jiabei Zhou, Xianzhu Zhong, Kenji Takada, Masayuki Yamaguchi and Tatsuo Kaneko
Polymers 2024, 16(24), 3563; https://doi.org/10.3390/polym16243563 - 20 Dec 2024
Viewed by 1035
Abstract
Polybenzimidazole (PBI) is a high-performance polymer known for its excellent thermal stability, mechanical strength, and chemical resistance, attributes that are derived from its unique structure comprising repeated benzene and imidazole rings. However, limitations such as relatively low thermal stability and moisture sensitivity restrict [...] Read more.
Polybenzimidazole (PBI) is a high-performance polymer known for its excellent thermal stability, mechanical strength, and chemical resistance, attributes that are derived from its unique structure comprising repeated benzene and imidazole rings. However, limitations such as relatively low thermal stability and moisture sensitivity restrict its application as a super engineering plastic. In this study, amide groups are incorporated into the PBI backbone to synthesize the copolymer poly(BI-co-A), effecting a structural modification at the molecular level. Additionally, silica nanospheres were composited into the poly(BI-co-A) film to further enhance its thermal performance. The resulting composite films exhibited remarkable thermal stability, with the temperature for 10% weight loss reaching as high as 761 °C. To address increased water absorption due to the high hydrophilicity of hydroxyl groups on the silica nanospheres’ surface, a dehydration treatment was applied in an electric furnace. This treatment produced a highly thermoresistant poly(BI-co-A) nanocomposite film with reduced wettability, making it suitable for applications in humid environments. Full article
(This article belongs to the Special Issue Advances in High-Performance Polymer Materials)
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18 pages, 2873 KiB  
Article
Improving Resistive Heating, Electrical and Thermal Properties of Graphene-Based Poly(Vinylidene Fluoride) Nanocomposites by Controlled 3D Printing
by Rumiana Kotsilkova, Vladimir Georgiev, Mariya Aleksandrova, Todor Batakliev, Evgeni Ivanov, Giovanni Spinelli, Rade Tomov and Tsvetozar Tsanev
Nanomaterials 2024, 14(22), 1840; https://doi.org/10.3390/nano14221840 - 17 Nov 2024
Viewed by 1870
Abstract
This study developed a novel 3D-printable poly(vinylidene fluoride) (PVDF)-based nanocomposite incorporating 6 wt% graphene nanoplatelets (GNPs) with programmable characteristics for resistive heating applications. The results highlighted the significant effect of a controlled printing direction (longitudinal, diagonal, and transverse) on the electrical, thermal, Joule [...] Read more.
This study developed a novel 3D-printable poly(vinylidene fluoride) (PVDF)-based nanocomposite incorporating 6 wt% graphene nanoplatelets (GNPs) with programmable characteristics for resistive heating applications. The results highlighted the significant effect of a controlled printing direction (longitudinal, diagonal, and transverse) on the electrical, thermal, Joule heating, and thermo-resistive properties of the printed structures. The 6 wt% GNP/PVDF nanocomposite exhibited a high electrical conductivity of 112 S·m−1 when printed in a longitudinal direction, which decreased significantly in other directions. The Joule heating tests confirmed the material’s efficiency in resistive heating, with the maximum temperature reaching up to 65 °C under an applied low voltage of 2 V at a raster angle of printing of 0°, while the heating Tmax decreased stepwise with 10 °C at the 45° and the 90° printing directions. The repeatability of the Joule heating performance was verified through multiple heating and cooling cycles, demonstrating consistent maximum temperatures across several tests. The effect of sample thickness, controlled by the number of printed layers, was investigated, and the results underscore the advantages of programmable 3D printing orientation in thin layers for enhanced thermal stability, tailored electrical conductivity, and efficient Joule heating capabilities of 6 wt% GNP/PVDF composites, positioning them as promising candidates for next-generation 3D-printed electronic devices and self-heating applications. Full article
(This article belongs to the Special Issue Hybrid Nano Polymer Composites (2nd Edition))
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16 pages, 1374 KiB  
Article
Effect of Thawing Procedure and Thermo-Resistance Test on Sperm Motility and Kinematics Patterns in Two Bovine Breeds
by Juan M. Solís, Francisco Sevilla, Miguel A. Silvestre, Ignacio Araya-Zúñiga, Eduardo R. S. Roldan, Alejandro Saborío-Montero and Anthony Valverde
Animals 2024, 14(19), 2768; https://doi.org/10.3390/ani14192768 - 25 Sep 2024
Cited by 1 | Viewed by 2201
Abstract
This investigation aimed to analyze the effect that thawing time and temperature in combination with a termo-resistance test had on straws from dairy bulls used for artificial insemination (AI) on semen motility and kinematic variables measured with CASA systems. Eight animals of Holstein [...] Read more.
This investigation aimed to analyze the effect that thawing time and temperature in combination with a termo-resistance test had on straws from dairy bulls used for artificial insemination (AI) on semen motility and kinematic variables measured with CASA systems. Eight animals of Holstein and Jersey breeds were used, and nine frozen-thawed semen doses per animal were analyzed for each breed. Three temperatures (35, 37, and 40 °C) and three thawing times (35, 40, and 45 s) were evaluated using a factorial design. Motility and kinematic patterns were analyzed using CASA-mot (Computer-Assisted Semen Analysis of motility) technology at different post-thawing times (0.5, 1, and 2 h). Sperm motility in Jersey bulls was higher (p < 0.05) than in Holstein ones (64.52 ± 1.45% and 53.10 ± 1.40%, respectively). The same effect was seen with progressive motility among the two breeds (Jersey: 45.29 ± 1.00%; Holstein: 36.30 ± 0.98%, p < 0.05). The Jersey breed presented higher values (p < 0.05) of curvilinear velocity (VCL), rectilinear velocity (VSL), average velocity (VAP), linearity on forward progression (LIN), and wobble (WOB). The Holstein breed showed a lower mean value (p < 0.05) of the beat-cross frequency (BCF) compared to the Jersey breed, thus suggesting an effect on VCL and VAP. During the post-thaw period, a gradual increase in VCL was observed at 2 h. VSL and VAP showed a decrease (p < 0.05) as the post-thaw period was prolonged. The study showed differences in sperm quality between Holstein and Jersey breeds, influenced by cryopreservation, thawing, and post-thawing incubation. Thawing at 37 °C for 30 s was considered optimal in relation to sperm motility. In addition, a decrease in sperm quality was observed as post-thawing time increased. Full article
(This article belongs to the Special Issue Technological Applications in Farm Animal Reproduction)
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13 pages, 5021 KiB  
Article
Towards Sustainable Temperature Sensor Production through CO2-Derived Polycarbonate-Based Composites
by Ane Martín-Ayerdi, Luis Rubio-Peña, Nikola Peřinka, Itziar Oyarzabal, José L. Vilas, Pedro Costa and Senentxu Lanceros-Méndez
Polymers 2024, 16(13), 1948; https://doi.org/10.3390/polym16131948 - 8 Jul 2024
Viewed by 1443
Abstract
The steep increase in carbon dioxide (CO2) emissions has created great concern due to its role in the greenhouse effect and global warming. One approach to mitigate CO2 levels involves its application in specific technologies. In this context, CO2 [...] Read more.
The steep increase in carbon dioxide (CO2) emissions has created great concern due to its role in the greenhouse effect and global warming. One approach to mitigate CO2 levels involves its application in specific technologies. In this context, CO2 can be used for a more sustainable synthesis of polycarbonates (CO2-PCs). In this research, CO2-PC films and composites with multiwalled carbon nanotubes (MWCNTs, ranging from 0.2 to 7.0 wt.%) have been prepared to achieve more sustainable multifunctional sensing devices. The inclusion of the carbonaceous fillers allows for the electrical conductivity to be enhanced, reaching the percolation threshold (Pc) at 0.1 wt.% MWCNTs and a maximum electrical conductivity of 0.107 S·m−1 for the composite containing 1.5 wt.% MWCNTs. The composite containing 3.0 wt.% MWCNTs was also studied, showing a stable and linear response under temperature variations from 40 to 100 °C and from 30 to 45 °C, with a sensitivity of 1.3 × 10−4 °C−1. Thus, this investigation demonstrates the possibility of employing CO2-derived PC/MWCNT composites as thermoresistive sensing materials, allowing for the transition towards sustainable polymer-based electronics. Full article
(This article belongs to the Special Issue Conductive and Magnetic Properties of Polymer Nanocomposites)
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18 pages, 7105 KiB  
Article
PVDF Hybrid Nanocomposites with Graphene and Carbon Nanotubes and Their Thermoresistive and Joule Heating Properties
by Stiliyana Stoyanova, Evgeni Ivanov, Lohitha R. Hegde, Antonia Georgopoulou, Frank Clemens, Fahmi Bedoui and Rumiana Kotsilkova
Nanomaterials 2024, 14(11), 901; https://doi.org/10.3390/nano14110901 - 21 May 2024
Cited by 5 | Viewed by 2158
Abstract
In recent years, conductive polymer nanocomposites have gained significant attention due to their promising thermoresistive and Joule heating properties across a range of versatile applications, such as heating elements, smart materials, and thermistors. This paper presents an investigation of semi-crystalline polyvinylidene fluoride (PVDF) [...] Read more.
In recent years, conductive polymer nanocomposites have gained significant attention due to their promising thermoresistive and Joule heating properties across a range of versatile applications, such as heating elements, smart materials, and thermistors. This paper presents an investigation of semi-crystalline polyvinylidene fluoride (PVDF) nanocomposites with 6 wt.% carbon-based nanofillers, namely graphene nanoplatelets (GNPs), multi-walled carbon nanotubes (MWCNTs), and a combination of GNPs and MWCNTs (hybrid). The influence of the mono- and hybrid fillers on the crystalline structure was analyzed by X-ray diffraction (XRD) and differential scanning calorimetry (DSC). It was found that the nanocomposites had increased amorphous fraction compared to the neat PVDF. Furthermore, nanocomposites enhanced the β phase of the PVDF by up to 12% mainly due to the presence of MWCNTs. The resistive properties of the nanocompositions were weakly affected by the temperature in the analyzed temperature range of 25–100 °C; nevertheless, the hybrid filler composites were proven to be more sensitive than the monofiller ones. The Joule heating effect was observed when 8 and 10 V were applied, and the compositions reached a self-regulating effect at around 100–150 s. In general, the inclusion in PVDF of nanofillers such as GNPs and MWCNTs, and especially their hybrid combinations, may be successfully used for tuning the self-regulated Joule heating properties of the nanocomposites. Full article
(This article belongs to the Special Issue Hybrid Nano Polymer Composites (2nd Edition))
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11 pages, 1227 KiB  
Article
Acetic Fermentation of Cagaita Pulp: Technological and Chemical Characteristics
by Jeisa Farias De Sousa Santana, Guilherme Freitas de Lima Hercos, Josemar Gonçalves de Oliveira Filho, Daiane Costa dos Santos, Marilene Silva Oliveira, Bheatriz Silva Morais de Freitas, Fabiano Guimarães Silva and Mariana Buranelo Egea
Beverages 2024, 10(2), 28; https://doi.org/10.3390/beverages10020028 - 12 Apr 2024
Cited by 2 | Viewed by 1903
Abstract
The Brazilian Cerrado region has a rich plant diversity, with fruits that have peculiar and unique sensory characteristics. For these reasons, using these fruits for biotechnological production is a promising alternative, mainly to protect this biome from deforestation and degradation. The production of [...] Read more.
The Brazilian Cerrado region has a rich plant diversity, with fruits that have peculiar and unique sensory characteristics. For these reasons, using these fruits for biotechnological production is a promising alternative, mainly to protect this biome from deforestation and degradation. The production of fermented acetic acid is an option to add value to native fruits and offer the market beverages with better nutritional quality and bioactive compounds. This work aimed to characterize fruits and to develop cagaita (Eugenia dysenterica DC.) acetic fermented beverage. The fruits were subjected to physical-chemical analyses in the first part. Subsequently, different treatments for fermentation were tested using two types of enzymes (amylase and pectinase), two subspecies of Saccharomyces cerevisiae yeast (UFLA CA11 and thermoresistant LNF Angel), and the chaptalization of the must with sucrose (16 °Brix). Alcoholic fermentation was carried out in an incubator with temperature control at 34 ± 1 °C. The pH, total soluble solids, titratable acidity, alcohol content, and density of the fermented products were monitored daily. The chaptalized must with amylase addition and thermoresistant yeast had the best performance during alcoholic fermentation, demonstrating that thermoresistant yeast is an economically advantageous and efficient alternative for the cagaita juice fermentation process. Subsequently, acetic fermentation was carried out using the slow method. Heat-resistant yeast without added enzymes was used to produce cagaita acetic fermented beverages within the parameters of the Brazilian legislation. Furthermore, phenolic compounds and antioxidant activity in the final product were observed. The work demonstrated the possibility of using cagaita fruits in biotechnological processes to produce new food products. Full article
(This article belongs to the Section Beverage Technology Fermentation and Microbiology)
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17 pages, 5135 KiB  
Article
Kaempferol as an Alternative Cryosupplement for Bovine Spermatozoa: Cytoprotective and Membrane-Stabilizing Effects
by Štefan Baňas, Eva Tvrdá, Filip Benko, Michal Ďuračka, Natália Čmiková, Norbert Lukáč and Miroslava Kačániová
Int. J. Mol. Sci. 2024, 25(7), 4129; https://doi.org/10.3390/ijms25074129 - 8 Apr 2024
Cited by 4 | Viewed by 2231
Abstract
Kaempferol (KAE) is a natural flavonoid with powerful reactive oxygen species (ROS) scavenging properties and beneficial effects on ex vivo sperm functionality. In this paper, we studied the ability of KAE to prevent or ameliorate structural, functional or oxidative damage to frozen–thawed bovine [...] Read more.
Kaempferol (KAE) is a natural flavonoid with powerful reactive oxygen species (ROS) scavenging properties and beneficial effects on ex vivo sperm functionality. In this paper, we studied the ability of KAE to prevent or ameliorate structural, functional or oxidative damage to frozen–thawed bovine spermatozoa. The analysis focused on conventional sperm quality characteristics prior to or following thermoresistance tests, namely the oxidative profile of semen alongside sperm capacitation patterns, and the levels of key proteins involved in capacitation signaling. Semen samples obtained from 30 stud bulls were frozen in the presence of 12.5, 25 or 50 μM KAE and compared to native ejaculates (negative control—CtrlN) as well as semen samples cryopreserved in the absence of KAE (positive control—CtrlC). A significant post-thermoresistance test maintenance of the sperm motility (p < 0.001), membrane (p < 0.001) and acrosome integrity (p < 0.001), mitochondrial activity (p < 0.001) and DNA integrity (p < 0.001) was observed following supplementation with all KAE doses in comparison to CtrlC. Experimental groups supplemented with all KAE doses presented a significantly lower proportion of prematurely capacitated spermatozoa (p < 0.001) when compared with CtrlC. A significant decrease in the levels of the superoxide radical was recorded following administration of 12.5 (p < 0.05) and 25 μM KAE (p < 0.01). At the same time, supplementation with 25 μM KAE in the cryopreservation medium led to a significant stabilization of the activity of Mg2+-ATPase (p < 0.05) and Na+/K+-ATPase (p < 0.0001) in comparison to CtrlC. Western blot analysis revealed that supplementation with 25 μM KAE in the cryopreservation medium prevented the loss of the protein kinase A (PKA) and protein kinase C (PKC), which are intricately involved in the process of sperm activation. In conclusion, we may speculate that KAE is particularly efficient in the protection of sperm metabolism during the cryopreservation process through its ability to promote energy synthesis while quenching excessive ROS and to protect enzymes involved in the process of sperm capacitation and hyperactivation. These properties may provide supplementary protection to spermatozoa undergoing the freeze–thaw process. Full article
(This article belongs to the Special Issue A Molecular Perspective on Reproductive Health)
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23 pages, 1622 KiB  
Article
Toxicity Screening of Fungal Extracts and Metabolites, Xenobiotic Chemicals, and Indoor Dusts with In Vitro and Ex Vivo Bioassay Methods
by Tuomas Hintikka, Maria A. Andersson, Taina Lundell, Tamás Marik, László Kredics, Raimo Mikkola, Magnus C. Andersson, Jarek Kurnitski and Heidi Salonen
Pathogens 2024, 13(3), 217; https://doi.org/10.3390/pathogens13030217 - 29 Feb 2024
Cited by 1 | Viewed by 4453
Abstract
It is controversial how useful bioassays are for identifying the in vivo toxicity of hazardous environmental exposures. In this study, fruiting bodies of forest mushrooms (n = 46), indoor mold colonies (n = 412), fungal secondary metabolites (n = 18), xenobiotic chemicals such [...] Read more.
It is controversial how useful bioassays are for identifying the in vivo toxicity of hazardous environmental exposures. In this study, fruiting bodies of forest mushrooms (n = 46), indoor mold colonies (n = 412), fungal secondary metabolites (n = 18), xenobiotic chemicals such as biocides and detergents (n = 6), and methanol extracts of indoor dusts from urban buildings (n = 26) were screened with two different bioactivity assays: boar sperm motility inhibition (BSMI) and inhibition of cell proliferation (ICP) tests. For the forest mushrooms, the toxicity testing result was positive for 100% of poisonous-classified species, 69% of non-edible-classified species, and 18% of edible-classified species. Colonies of 21 isolates of Ascomycota mold fungal species previously isolated from water-damaged buildings proved to be toxic in the tests. Out of the fungal metabolites and xenobiotic chemicals, 94% and 100% were toxic, respectively. Out of the indoor dusts from moldy-classified houses (n = 12) and from dry, mold-free houses (n = 14), 50% and 57% were toxic, respectively. The bioassay tests, however, could not differentiate the samples from indoor dusts of moldy-classified buildings from those from the mold-free buildings. Xenobiotic chemicals and indoor dusts were more toxic in the BSMI assay than in the ICP assay, whereas the opposite results were obtained with the Ascomycota mold colonies and fungal secondary metabolites. The tests recognized unknown methanol-soluble thermoresistant substances in indoor settled dusts. Toxic indoor dusts may indicate a harmful exposure, regardless of whether the toxicity is due to xenobiotic chemicals or microbial metabolites. Full article
(This article belongs to the Special Issue Detection of Indoor Fungi: Part II)
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13 pages, 2401 KiB  
Article
Incorporation of Aramids into Polybenzimidazoles to Achieve Ultra-High Thermoresistance and Toughening Effects
by Xianzhu Zhong, Aniruddha Nag, Kenji Takada, Akinori Nakajima and Tatsuo Kaneko
Molecules 2024, 29(5), 1058; https://doi.org/10.3390/molecules29051058 - 28 Feb 2024
Cited by 2 | Viewed by 1552
Abstract
Polybenzimidazoles (PBIs) are recognized for their remarkable thermal stability due to their unique molecular structure, which is characterized by aromaticity and rigidity. Despite their remarkable thermal attributes, their tensile properties limit their application. To improve the mechanical performance of PBIs, we made a [...] Read more.
Polybenzimidazoles (PBIs) are recognized for their remarkable thermal stability due to their unique molecular structure, which is characterized by aromaticity and rigidity. Despite their remarkable thermal attributes, their tensile properties limit their application. To improve the mechanical performance of PBIs, we made a vital modification to their molecular backbone to improve their structural flexibility. Non-π-conjugated components were introduced into PBIs by grafting meta-polyamide (MA) and para-polyamide (PA) onto PBI backbones to form the copolymers PBI-co-MA and PBI-co-PA. The results indicated that the cooperation between MA and PA significantly enhanced mechanical strain and overall toughness. Furthermore, the appropriate incorporation of aromatic polyamide components (20 mol% for MA and 15% for PA) improved thermal degradation temperatures by more than 30 °C. By investigating the copolymerization of PBIs with MA and PA, we unraveled the intricate relationships between composition, molecular structure, and material performance. These findings advance copolymer design strategies and deepen the understanding of polymer materials, offering tailored solutions that address thermal and mechanical demands across applications. Full article
(This article belongs to the Special Issue Themed Issue Dedicated to Prof. Bernard Boutevin)
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16 pages, 10049 KiB  
Article
Oxy-Butane Ablation Testing of Thermal Protection Systems Based on Nanomodified Phenolic Resin Matrix Materials
by George Pelin, Cristina Elisabeta Pelin, Adriana Stefan, Violeta Tsakiris, Alexandra Ana Maria Panait and Emil Costea
Polymers 2023, 15(19), 4016; https://doi.org/10.3390/polym15194016 - 7 Oct 2023
Cited by 2 | Viewed by 2083
Abstract
Two classes of thermal protection systems composed of a carbon-fibre-reinforced (CFRP) layer and an ablative material layer joined with a thermo-resistant ceramic adhesive were developed. The two classes differ in the composition of the ablative material reinforcing compound. In the first class, the [...] Read more.
Two classes of thermal protection systems composed of a carbon-fibre-reinforced (CFRP) layer and an ablative material layer joined with a thermo-resistant ceramic adhesive were developed. The two classes differ in the composition of the ablative material reinforcing compound. In the first class, the ablative material is based on micronic-sized cork granules, and in the second class, the ablative material is reinforced with carbonic felt. For both classes of thermal protection systems, the reinforcement material was impregnated in simple phenolic resin, and nanometric additive, consisting of silicon carbide nanoparticles added in two different weight contents (1 and 2% by weight) relative to the resin. The thermal conductivity for the ablative materials in the thermal protection systems structure was determined. A test facility using oxy-butane flame was developed through which the thermal protection systems developed were tested at extreme temperatures, to simulate some thermal conditions in space applications. The materials were characterised from a morphostructural point of view using optical and scanning electron microscopy after thermal testing. The TPS composed of the carbon-felt-based ablative layer showed improved behaviour compared to the cork-based ablative ones in terms of the temperature increase rate during thermal conductivity testing, mass loss, as well as morphostructural appearance and material erosion after oxy-butane testing. The nSiC-based samples in both sets of TPSs showed improved behaviour compared to the un-filled ones, considering the temperature increase, mass loss, and morphostructure of the eroded material. Full article
(This article belongs to the Special Issue High-Temperature Composite Resin)
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15 pages, 3310 KiB  
Article
Directed Modification of a GHF11 Thermostable Xylanase AusM for Enhancing Inhibitory Resistance towards SyXIP-I and Application of AusMPKK in Bread Making
by Dong Zhang, Jing Huang, Youyi Liu, Xingyi Chen, Tiecheng Gao, Ning Li, Weining Huang and Minchen Wu
Foods 2023, 12(19), 3574; https://doi.org/10.3390/foods12193574 - 26 Sep 2023
Cited by 6 | Viewed by 1724
Abstract
To reduce the inhibition sensitivity of a thermoresistant xylanase AusM to xylanase inhibitor protein (XIP)-type in wheat flour, the site-directed mutagenesis was conducted based on the computer-aided redesign. First, fourteen single-site variants and one three-amino acid replacement variant in the thumb region of [...] Read more.
To reduce the inhibition sensitivity of a thermoresistant xylanase AusM to xylanase inhibitor protein (XIP)-type in wheat flour, the site-directed mutagenesis was conducted based on the computer-aided redesign. First, fourteen single-site variants and one three-amino acid replacement variant in the thumb region of an AusM-encoding gene (AusM) were constructed and expressed in E. coli BL21(DE3), respectively, as predicted theoretically. At a molar ratio of 100:1 between SyXIP-I/xylanase, the majority of mutants were nearly completely inactivated by the inhibitor SyXIP-I, whereas AusMN127A retained 62.7% of its initial activity and AusMPKK retained 100% of its initial activity. The optimal temperature of the best mutant AusMPKK was 60 °C, as opposed to 60–65 °C for AusM, while it exhibited improved thermostability, retaining approximately 60% of its residual activity after heating at 80 °C for 60 min. Furthermore, AusMPKK at a dosage of 1000 U/kg was more effective than AusM at 4000 U/kg in increasing specific bread loaf volume and reducing hardness during bread production and storage. Directed evolution of AusM significantly reduces inhibition sensitivity, and the mutant enzyme AusMPKK is conducive to improving bread quality and extending its shelf life. Full article
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34 pages, 1608 KiB  
Article
Quantitative Measurement of Thermal Conductivity by SThM Technique: Measurements, Calibration Protocols and Uncertainty Evaluation
by Nolwenn Fleurence, Séverine Demeyer, Alexandre Allard, Sarah Douri and Bruno Hay
Nanomaterials 2023, 13(17), 2424; https://doi.org/10.3390/nano13172424 - 25 Aug 2023
Cited by 3 | Viewed by 2704
Abstract
Thermal management is a key issue for the downsizing of electronic components in order to optimise their performance. These devices incorporate more and more nanostructured materials, such as thin films or nanowires, requiring measurement techniques suitable to characterise thermal properties at the nanoscale, [...] Read more.
Thermal management is a key issue for the downsizing of electronic components in order to optimise their performance. These devices incorporate more and more nanostructured materials, such as thin films or nanowires, requiring measurement techniques suitable to characterise thermal properties at the nanoscale, such as Scanning Thermal Microscopy (SThM). In active mode, a hot thermoresistive probe scans the sample surface, and its electrical resistance R changes as a function of heat transfers between the probe and sample. This paper presents the measurement and calibration protocols developed to perform quantitative and traceable measurements of thermal conductivity k using the SThM technique, provided that the heat transfer conditions between calibration and measurement are identical, i.e., diffusive thermal regime for this study. Calibration samples with a known k measured at the macroscale are used to establish the calibration curve linking the variation of R to k. A complete assessment of uncertainty (influencing factors and computational techniques) is detailed for both the calibration parameters and the estimated k value. Outcome analysis shows that quantitative measurements of thermal conductivity with SThM (with an uncertainty value of 10%) are limited to materials with low thermal conductivity (k<10Wm1K1). Full article
(This article belongs to the Special Issue Metrology for Energy Nanomaterials)
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16 pages, 18271 KiB  
Article
Development and Characterization of Hybrid, Temperature Sensing and Heating Yarns with Color Change
by Theresa Junge, Rike Brendgen, Carsten Grassmann, Thomas Weide and Anne Schwarz-Pfeiffer
Sensors 2023, 23(16), 7076; https://doi.org/10.3390/s23167076 - 10 Aug 2023
Cited by 7 | Viewed by 2298
Abstract
A person’s body temperature is an important indicator of their health status. A deviation of that temperature by just 2 °C already has or can lead to serious consequences, such as fever or hypothermia. Hence, the development of a temperature-sensing and heatable yarn [...] Read more.
A person’s body temperature is an important indicator of their health status. A deviation of that temperature by just 2 °C already has or can lead to serious consequences, such as fever or hypothermia. Hence, the development of a temperature-sensing and heatable yarn is an important step toward enabling and improving the monitoring and regulation of a person’s body temperature. This technology offers benefits to several industries, such as health care and sports. This paper focuses on the characterization and development of a hybrid yarn, which can measure and visualize temperature changes through a thermoresistive and thermochromic effect. Moreover, the yarn is able to serve as a flexible heating element by connecting to a power source. The structure of the yarn is designed in three layers. Each layer and component ensures the functionality and flexibility of the yarn and additional compatibility with further processing steps. A flexible stainless steel core was used as the heat-sensitive and heat-conducting material. The layer of polyester wrapped around the stainless steel yarn improves the wearing comfort and serves as substrate material for the thermochromic coating. The resulting hybrid yarn has a reproducible sensory function and changes its resistance by 0.15 Ω between 20 and 60 °C for a length of 30 cm. In addition, the yarn has a uniform and reproducible heating power, so that temperature steps can be achieved at a defined length by selecting certain voltages. The thermochromic color change is clearly visible between 28 and 29 °C. Due to its textile structure, the hybrid sensory and actuating yarn can easily be incorporated into a woven fabric or into a textile by means of joining technology sewing. Full article
(This article belongs to the Section Nanosensors)
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13 pages, 5580 KiB  
Article
Discovery and Functional Evaluation of Heat Tolerance Genes in the Nonconventional Yeast Yarrowia lipolytica
by Mengchan Liu and Hairong Cheng
Fermentation 2023, 9(6), 509; https://doi.org/10.3390/fermentation9060509 - 25 May 2023
Cited by 3 | Viewed by 2264
Abstract
Yarrowia lipolytica, a GRAS (generally recognized as safe) nonconventional yeast, has been used widely in industrial fermentation to produce chemicals, fuels, and functional sugars such as erythritol and mannitol. Although Y. lipolytica is a promising organism for bioconversion and has substantial potential [...] Read more.
Yarrowia lipolytica, a GRAS (generally recognized as safe) nonconventional yeast, has been used widely in industrial fermentation to produce chemicals, fuels, and functional sugars such as erythritol and mannitol. Although Y. lipolytica is a promising organism for bioconversion and has substantial potential in industrial production, its utilization is restricted by the high cost of cooling during the fermentation process; the optimum growth or fermentation temperature of Y. lipolytica is 28–30 °C, which is lower than that of some fermenting species. Therefore, it is necessary to breed a thermoresistant Y. lipolytica for use in a fermentation system. Here, we report a new thermoduric Y. lipolytica strain (a thermoresistant clone, HRC) that can grow at 35 °C—higher than the starting strain Y. lipolytica CGMCC7326 (maximum growth temperature at 33 °C)—by laboratory adaptive evolution. Based on the transcriptome analysis of the mutant strain HRC and the parental strain Y. lipolytica CGMCC7326 at different temperatures, 22 genes with increased expression at high temperatures were identified and 10 of them were overexpressed in Y. lipolytica CGMCC7326. HRC1, HRC2, and HRC3 (with YALI0B21582g, YALI0C13750g, and YALI0B10626g overexpressed, respectively) were assessed for growth at higher temperatures. This revealed that these three genes were related to thermotolerance. This study provides insights into the metabolic landscape of Y. lipolytica under heat stress, enabling future metabolic engineering endeavors to improve both thermoresistance and sugar alcohol production in the yeast Y. lipolytica. Full article
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