Search Results (19)

As an important component of the global fishery economy, the crab breeding and processing industry faces the dual challenges of sustainable development and technological upgrading. This paper first systematically analyzes the regional distribution and core biological characteristics of major global economic crab species, laying a foundation for the targeted design of processing technologies and equipment. Secondly, based on advances in crab processing technology, the industry is categorized into two systems: live crab processing and dead crab processing. Live crab processing has formed a full-chain technological system of “fishing–temporary rearing–depuration–grading–packaging”. Dead crab processing focuses on high-value utilization: high-pressure processing enhances the quality of crab meat; liquid nitrogen quick-freezing combined with modified atmosphere packaging extends shelf life; and biological fermentation and enzymatic hydrolysis facilitate the green extraction of chitin from crab shells. In terms of intelligent equipment application, sensor technology enables full coverage of aquaculture water quality monitoring, precise classification during processing, and vitality monitoring during transportation. Automation technology reduces labor costs, while fuzzy logic algorithms ensure the process stability of crab meat products. The integration of the Internet of Things (IoT) and big data analytics, combined with blockchain technology, enables full-link traceability of the “breeding–processing–transportation” chain. In the future, cross-domain technological integration and multi-equipment collaboration will be the key to promoting the sustainable development of the industry. Additionally, with the support of big data and artificial intelligence, precision management of breeding, processing, logistics, and other links will realize a more efficient and environmentally friendly crab industry model. Full article

Soiling is one of the main performance risks for bifacial photovoltaic (PV) technology, particularly in arid environments such as the Atacama Desert, where dust is deposited asymmetrically on the front and rear surfaces of the modules. This study evaluates one year (July 2022 to June 2023) of soiling behavior in bifacial modules installed in fixed-tilt and horizontal single-axis tracking (HSAT) configurations, enabling a comparison to be made between static and moving structures. The average dust accumulation was found to be 0.33 mg/cm² on the front surface and 0.15 mg/cm² on the rear surface of the fixed modules. In contrast, the respective values for the HSAT systems were found to be lower at 0.25 mg/cm² and 0.035 mg/cm². These differences resulted in performance losses of 5.8% for fixed modules and 3.7% for HSAT systems. Microstructural analysis revealed that wetting and drying cycles had formed dense, cemented layers on the front surface of fixed modules, whereas tracking modules exhibited looser deposits. Natural cleaning events, such as fog, dew and frost, only provided partial and temporary mitigation. These findings demonstrate that bifaciality introduces differentiated soiling dynamics between the front and rear surfaces, emphasizing the importance of tailored cleaning strategies and the integration of monitoring systems that consider bifacial gain as a key operational parameter. These insights are crucial for developing predictive models and cost-effective O&M strategies in large-scale bifacial PV deployments under desert conditions. Full article

Due to its high bioconversion efficiency and nutritional value, the black soldier fly (Hermetia illucens L. 1758) is a promising insect species for sustainable animal feed production. However, concerns remain regarding microbial safety when larvae are reared on substrates contaminated by pathogenic or spoilage bacteria. This study investigated the effects of substrate inoculation with Bacillus subtilis 168 or Pseudomonas putida KT2440 on larval performance and gut microbiota composition. Larvae reared on contaminated diets showed no significant differences in survival or development time compared to controls. However, a short-term reduction in growth was observed in the Bacillus-exposed group. qPCR analyses confirmed the temporary presence of Bacillus taxa in larval guts, while Pseudomonas taxa were effectively excluded. Amplicon sequencing of the 16S rRNA gene revealed that the contamination did not affect gut bacterial microbiota richness and composition. Instead, the bacterial communities evolved naturally with Lactobacillales-related bacteria dominating early stages and Morganellaceae taxa becoming more abundant in prepupae. Our findings demonstrate the stability and resilience of H. illucens gut bacterial microbiota, reinforcing the safety and suitability of H. illucens as a feed ingredient, even when reared under challenging microbial conditions. Full article

For the local oscillation phenomenon of the APF algorithm in the face of static U-shaped obstacles, the path cusp phenomenon caused by the vehicle corner and path curvature constraints is not taken into account, as well as the low path safety caused by ignoring the vehicle volume constraints. Therefore, an APF-Dijkstra path planning fusion algorithm based on steering model and volume constraints is proposed to improve it. First, perform an expansion treatment on the obstacles in the map, optimize the search direction of the Dijkstra algorithm and its planned global path, ensuring that the distance between the path and the expanded grid is no less than 1 m, and use the path points as temporary target points for the APF algorithm. Secondly, a Gaussian function is introduced to optimize the potential energy function of the APF algorithm, and the U-shaped obstacle is ellipticized, and a virtual target point is used to provide the gravitational force. Again, the three-point arc method based on the steering model is used to determine the location of the predicted points and to smooth the paths in real time while constraining the steering angle. Finally, a 4.5 m × 2.5 m vehicle rectangle is used instead of the traditional mass points to make the algorithm volumetrically constrained. Meanwhile, a model for detecting vehicle collisions is established to cover the rectangle boundary with 14 envelope circles, and the combined force of the computed mass points is transformed into the combined force of the computed envelope circles to further improve path safety. The algorithm is validated by simulation experiments, and the results show that the fusion algorithm can avoid static U-shaped obstacles and dynamic obstacles well; the curvature change rate of the obstacle avoidance path is 0.248, 0.162, and 0.169, and the curvature standard deviation is 0.16, which verifies the smoothness of the fusion algorithm. Meanwhile, the distances between the obstacles and the center of the rear axle of the vehicle are all higher than 1.60 m, which verifies the safety of the fusion algorithm. Full article

This study investigated the effects of dietary Antarctic krill meal (AKM) on the physiological metabolism and flavor quality of adult Eriocheir sinensis ovaries during the postharvest temporary rearing. The AKM concentrations tested were 0% (including negative control group and positive control group), 2%, 4%, 6%, and 8%. The results indicate that the E. sinensis ovaries in 8% AKM group produced the highest levels of aroma compounds after thermal processing, including hexanal, heptanal, phenylacetaldehyde, 3-octanone, and 2-methylbutanoic acid ethyl ester. The 8% AKM and negative control group were analyzed by UPLC-MS/MS combined with the nontargeted and widely targeted metabolomics technique. The AKM altered the composition of aroma precursors by adjusting the metabolism of glycerophospholipid, linoleic acid, α-linolenic acid, and amino acid in ovaries. Moreover, lipids composed of polyunsaturated fatty acids (PUFAs) were significantly upregulated (p < 0.05). Dietary supplementation with 8% AKM had the best effect on improving the ovarian flavor quality of E. sinensis. During the postharvest temporary rearing, more aromatic precursors were produced by regulating physiological metabolism. The ovarian flavor was enhanced by lipid oxidation, Maillard reaction, and Strecker degradation during thermal processing. Full article

In response to the difficulty of controlling the layered composite roof of large-section coal roadways and the problem of slow excavation speed caused by unreasonable support parameter values, a dynamic staged control principle for surrounding rock based on “high-strength passive temporary support near the excavation face, combined with active support of rear bolts and anchor cables” is proposed by analyzing the evolution law of rock release stress under the spatial effect of excavation face. Based on the geological conditions of the 1211 (1) transportation roadway in Guqiao Coal Mine, a similar physical simulation test model was constructed to conduct experimental research on the bearing capacity and deformation instability mechanism of the surrounding rock of the layered-composite-roof coal roadway. The law of influence of staged support on the deformation and failure evolution of the surrounding rock was obtained. The research results show the following: (1) After loading above the model, the vertical stress on the roof increases rapidly in a “stepped” manner. After unloading the roadway excavation, due to the release of constraints on the roof above the roadway, the vertical stress on the roof rapidly decreases, especially in the temporary support area where the reduction in vertical stress on the roof is most significant. (2) As the vertical load increases, the displacement curve of the roof gradually evolves into a “V” shape. The farther away from the center of the roadway, the smaller the subsidence of the roof. When loaded to 54.45 kN, the subsidence of the roof increases, indicating that the development of roof delamination cracks is faster, and delamination occurs between 12 cm and 22 cm above the roof. (3) With the continuous increase of axial load, cracks first appear around the roof and slightly sink. Then, the cracks gradually expand and penetrate, causing instability and failure of the roadway roof. When the mining stress reaches 54.45 kN, the middle part of the roadway roof in the axial direction breaks, and the cracks penetrate, resulting in overall collapse. Full article

Salinity is a significant environmental component that affects the physiological state of aquatic species. This study aimed to investigate whether water salinity had an impact on the biochemical properties and meat quality of adult Aplodinotus grunniens during temporary rearing of 7 days. Salinity caused increased osmotic pressure and antioxidant enzyme activities of Aplodinotus grunniens, which were attributed to the increase in the content of alanine and glutamate. It raised the hardness and shear force with an increase in salinity, leading to an increase in water-holding capacity. Salinity enhanced the DHA ratio with a decrease in the atherosclerotic index and thrombosis index. Combined with the increase in flavor amino acids and nucleotides, salinity enhanced the umami taste of Aplodinotus grunniens. These findings suggest that temporary rearing in salinity might be a practical approach to improving the meat quality of adult Aplodinotus grunniens. Full article

Feeding on mixed, alternating, or changing diets often favor insect development. With the aim to optimize mass rearing and use for the biological control of insect pests, we investigated the effects of various combinations of high-quality (the green peach aphid Myzus persicae) and low-quality (eggs of the grain moth Sitotroga cerealella) foods on the larval development of a predatory ladybird Cheilomenes propinqua. In the first experiment, eggs and aphids were mixed in different proportions; in the second experiment, larvae switched from feeding on aphids to feeding on eggs. Although the beneficial additive effect of mixed foods was detected in some treatments with limited diets, feeding on various combinations of eggs with aphids never resulted in higher survival, faster development, or a larger size of emerging adults than those observed for feeding on unlimited amounts of aphids. For the practice of biological control, we conclude that, if necessary (for example, in the case of temporary shortage or a lack of aphids in mass rearing facilities or in the case of preventing release of C. propinqua adults in greenhouses), C. propinqua larvae can be fed with grain moth eggs by replacing, mixing, or alternating with aphids, although this will inevitably result in a proportional decrease in pre-adult survival, rate of development, weight, and size of the emerging adults. On the other hand, even a minimal addition of aphids can have a substantial positive effect on larvae fed with grain moth eggs. Full article

Long-term storage is an important component of insect mass-rearing systems, prolonging the shelf life of biocontrol agents during a low-demand period or a temporary lack of suitable food. Macrolophus pygmaeus is a predatory heteropteran, mass-reared and widely used for the biological control of arthropod pests in greenhouses. With the aim to determine the optimal conditions and acceptable duration of cold storage, we evaluated the impact of different periods of storage of fed and starved third instar nymphs of M. pygmaeus at different temperatures on nymphal survival, adult emergence, and female fecundity. Four storage temperatures (3, 6, 9, and 12 °C) were tested. The longevity of starved nymphs decreased with an increase in the storage temperature, with a maximum of about 40 days at 3 °C, whereas the longest lifetime of nymphs fed on eggs of the grain moth Sitotroga cerealella (about 150 days) was observed at 9 °C. Further experiments demonstrated that the third instar nymphs of M. pygmaeus fed with eggs of the grain moth can be stored at 9 °C for 30 days, with a moderate (10–20%) decrease in survival and fecundity, whereas the survival of starved nymphs decreased by half after 10 days of storage at 3 °C. Full article

Temporary works are necessary to ensure the construction and operation of railways. These works are characterized by their large scale, numerous locations, and long construction periods. However, suitable land resources for such purposes are extremely limited in mountainous railway areas. Additionally, the selection of sites for these works often overlaps with areas affected by debris flow, leading to high potential risks from geological disasters. Taking the Jinjia Gully watershed as an example, this paper explores a method for assessing debris flow risks in single gullies, including the zoning of debris flow danger areas, vulnerability analysis, and risk assessment. Based on the data obtained from field surveys, they utilize ArcGIS and the Analytic Hierarchy Process (AHP), combined with numerical simulations and indoor experiments, to establish a quantitative risk assessment method for large-scale temporary works. The results indicate that (1) the area of debris flow hazard zones decreases with increasing rainfall frequency, and (2) the vulnerability assessment model can not only reflect the types of individual work, structural materials, and construction quality but also the shielding effect of building clusters. In the direction of flow, the shielding effect range of buildings on debris flow accumulation fans is approximately 37.5 times the size of the buildings. In the direction of extension, when the angle between current and rear buildings exceeds 0.674 radians, the shielding effect can be neglected. (3) At a rainfall frequency of p = 5%, more than 80% of large-scale temporary works are in extremely low or low-risk zones, indicating that the study area is at a low risk level. Full article

We describe efforts to reintroduce the extinct-in-the-wild Spix’s Macaw (Cyanopsitta spixii) within its historical range in eastern Brazil. Twenty captive-reared Spix’s Macaws were released, along with 15 Blue-Winged Macaws (Primolius maracana), as heterospecific flocks in two events during the dry and rainy seasons of 2022. We monitored the release groups through daily observation and telemetry tracking from early June 2022 to early June 2023. We documented an overall first-year survival of 58.3% (CI: 37.8–78.7%), with 65% of Spix’s Macaws establishing a stable area of activity within 5 km of the release site, excluding any temporary long-distance forays. Eighty-five percent of released Spix’s Macaws exhibited flock cohesion, including interactions with and integration into wild Blue-Winged Macaw groups at the release site. Several released Spix’s Macaws formed pair-bonds with conspecifics and engaged in nest cavity exploration, breeding behavior, and territorial defense of nest sites, with three females also laying and incubating eggs. One nesting pair successfully hatched and reared chicks in an artificial nest cavity. These releases employed a novel reintroduction strategy using a surrogate species model, the sympatric Blue-Winged Macaw, to “mentor” and facilitate post-release adaptation by the target species and increase the total number of individuals in the release cohort. Lastly, participatory monitoring by local citizens is considered a way to engage and involve local communities in species and habitat conservation and potentially create new employment opportunities in the region. Full article

The aquaculture production of pikeperch has reached commercial scale in a number of European countries, but the high mortality of early life cycle stages and minor understanding of nutritional requirements are still major bottlenecks. To investigate the fate of fatty acids during early development, weaning and rearing, pikeperch larvae and juveniles from a commercial recirculating aquaculture system (RAS) were sampled over 2 months for morphometric data, as well as fatty acid composition, with a total of 6 sampling days, with four to five replicates per sampling day and between 1 and 25 pikeperch larvae per individual sample, depending on larval biomass. The biomass of sampled pikeperch larvae varied from 0.1 to 420 mg (dry mass DM), depending on the age of the larvae, and the initial length of the pikeperch larvae was about 4.5 mm. Our data confirm that, accompanied by an exponential increase in dry mass, total fatty acids (TFAs) in larval tissues increased with the beginning of exogenous feed uptake and were depleted between days 13 and 25 post hatch, most likely associated with the weaning and metamorphosis of the larvae. We conclude that all fatty acid classes may serve as metabolic fuel during metamorphosis, but the ultimate fatty acid composition is strongly impacted by the available feed. The chosen diet probably caused a lack of alpha-linolenic (18:3n-3; ALA) and docosahexaenoic acid (22:6n-3; DHA) during larval development and a shortage of vaccenic (18:1n-7), alpha-linolenic (18:3n-3; ALA) and arachidonic acid (20:4n-6; ARA) in juvenile pikeperch. This led to low DHA/EPA ratios 13 days post hatch, a high EPA/ARA ratio at days 41 and 56 post hatch and a fluctuating ratio of alpha-linolenic acid to linoleic acid (18:2n-6; LA). A temporary lack of essential fatty acids can cause dysfunctions and eventually mortalities in pikeperch larvae and juveniles. Despite high larval growth rates, the biochemical composition of the first fed Artemia and microdiets was most likely not sufficient and in need of improvement. We suggest that deficiencies must be compensated, e.g., through the substitution of the offered Artemia with more suitable live feed organisms, such as freshwater rotifers, and the enrichment of current microdiets in order to prevent high mortalities during pikeperch rearing and weaning. Full article

Growth patterns during early development of four species of riverine cyprinids, common dace (Leuciscus leuciscus L.), ide (Leuciscus idus L.), chub (Squalius cephalus L.) and common barbel (Barbus barbus L.), over 30 days of rearing, were analyzed. The study period covered three successive phases of larval development (preflexion, flexion, postflexion) and part of the juvenile development. The growth analysis carried out in the present study shows that significant allometric growth occurred mainly in preferentially localized body parts (head and tail) and was also associated with an increase in body height. The replacement of temporary structures (e.g., fin fold absorption) and the appearance of definitive structures around 21 mm TL (beginning of the juvenile period) corresponds to a stabilization of the relative growth of most parts of the body. This is probably due to the fact that the studied species had completed the most important part of the remodeling process (metamorphosis) and had achieved a body shape that allows juveniles to colonize faster water habitats. The results also indicate that individuals of species that are less developmentally advanced at the time of hatching (common barbel, chub) show greater initial dynamics of change in growth rate than individuals of species whose individuals are more advanced in ontogeny (common dace, ide). In addition, the small differences observed in growth parameters between successive periods analyzed, supported by the results of previous studies on these species, probably reflect the lack of abrupt changes in the behavior and habitat of the species studied. Full article

Organic selenium has been widely studied as a nutritional supplement for animal feed. However, there are few studies on the effect of organic selenium on flesh quality. In this study, the effects of organic selenium (yeast selenium (YS), Se 0.002 mg/L) on the metabolism and protein expression in Micropterus salmoides muscle under temporary fasting condition (6 weeks) were investigated. The muscle structure was observed through a microscope, and regulatory pathways were analyzed using proteomics and metabolomics methods. Electron microscopy showed that YS made the muscle fibers of M. salmoides more closely aligned. Differential analysis identified 523 lipid molecules and 268 proteins. The numbers of upregulated and downregulated proteins were 178 and 90, respectively, including metabolism (46.15%), cytoskeleton (11.24%) and immune oxidative stress (9.47%), etc. Integrated analyses revealed that YS enhanced muscle glycolysis, the tricarboxylic acid cycle and oxidative phosphorylation metabolism. In the YS group, the content of eicosapentaenoic acid was increased, and that of docosahexaenoic acid was decreased. YS slowed down protein degradation by downregulating ubiquitin and ubiquitin ligase expression. These results suggest that organic selenium can improve M. salmoides muscle quality through the aforementioned pathways, which provides potential insights into the improvement of the quality of aquatic products, especially fish. Full article

Industrial insect mass rearing aims to produce quality insects under safe sanitary conditions which can be compromised by pathogens and abiotic stressors. Therefore, knowledge on pathogen persistence, virulence and means of detection is of importance. This study focuses on the opportunistic pathogen Serratia marcescens (Sm) as a possible candidate to reveal sanitary issues in Tenebrio molitor (Tm) breeding. A screening test was performed to assess the impact of abiotic stressors (starvation, density and sieving) in presence and absence of Sm. Two Sm detection methods were conducted, and the kinetics of Sm persistence were investigated. Our results show that (i) the presence of Sm had a low but significant effect on Tm mortality, (ii) a short temporary starvation period had a negative impact on larval growth, (iii) the detection of Sm by q-PCR was sensitive but less convenient than a specific Sm growth media, (iv) the kinetics of persistence showed that Sm declined but survived for nine days in the feed and in the feces for three weeks. Both the relatively low virulence and the persistence in the environment suggest that Sm could be used as an indicator for the sanitary status of mealworm production. Full article