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Search Results (4,516)

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23 pages, 2002 KB  
Article
Risk Assessment of Coal Mine Ventilation System Based on Fuzzy Polymorphic Bayes: A Case Study of H Coal Mine
by Jin Zhao, Juan Shi and Jinhui Yang
Systems 2026, 14(1), 99; https://doi.org/10.3390/systems14010099 - 16 Jan 2026
Abstract
Coal mine ventilation systems face coupled and systemic risks characterized by structural interconnection and disaster chain propagation. In order to accurately quantify and evaluate this overall system risk, this study presents a new method of risk assessment of the coal mine ventilation system [...] Read more.
Coal mine ventilation systems face coupled and systemic risks characterized by structural interconnection and disaster chain propagation. In order to accurately quantify and evaluate this overall system risk, this study presents a new method of risk assessment of the coal mine ventilation system based on fuzzy polymorphic Bayesian networks. This method effectively addresses the shortcomings of traditional assessment approaches in the probabilistic quantification of risk. A Bayesian network with 44 nodes was established from five dimensions: ventilation power, ventilation network, ventilation facilities, human and management factors, and work environment. The risk states were divided into multiple states based on the As Low As Reasonably Practicable (ALARP) metric. The probabilities of evaluation-type root nodes were calculated using fuzzy evaluation, and the subjective bias was corrected by introducing a reliability coefficient. The concept of distance compensation is proposed to flexibly calculate the probabilities of quantitative-type root nodes. Through the verification of the ventilation system of H Coal Mine in Shanxi, China, it is concluded that the high risk of the ventilation system is 18%, and the high-risk probability of the ventilation system caused by the external air leakage of the mine is the largest. The evaluation results are consistent with real-world conditions. The results can provide a reference for improving the safety of the ventilation systems. Full article
(This article belongs to the Special Issue Advances in Reliability Engineering for Complex Systems)
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27 pages, 1134 KB  
Article
A Cryptocurrency Dual-Offline Payment Method for Payment Capacity Privacy Protection
by Huayou Si, Yaqian Huang, Guozheng Li, Yun Zhao, Yuanyuan Qi, Wei Chen and Zhigang Gao
Electronics 2026, 15(2), 400; https://doi.org/10.3390/electronics15020400 - 16 Jan 2026
Abstract
Current research on cryptocurrency dual-offline payment systems has garnered significant attention from both academia and industry, owing to its potential payment feasibility and application scalability in extreme environments and network-constrained scenarios. However, existing dual-offline payment schemes exhibit technical limitations in privacy preservation, failing [...] Read more.
Current research on cryptocurrency dual-offline payment systems has garnered significant attention from both academia and industry, owing to its potential payment feasibility and application scalability in extreme environments and network-constrained scenarios. However, existing dual-offline payment schemes exhibit technical limitations in privacy preservation, failing to adequately safeguard sensitive data such as payment amounts and participant identities. To address this, this paper proposes a privacy-preserving dual-offline payment method utilizing a cryptographic challenge-response mechanism. The method employs zero-knowledge proof technology to cryptographically protect sensitive information, such as the payer’s wallet balance, during identity verification and payment authorization. This provides a technical solution that balances verification reliability with privacy protection in dual-offline transactions. The method adopts the payment credential generation and credential verification mechanism, combined with elliptic curve cryptography (ECC), to construct the verification protocol. These components enable dual-offline functionality while concealing sensitive information, including counterparty identities and wallet balances. Theoretical analysis and experimental verification on 100 simulated transactions show that this method achieves an average payment generation latency of 29.13 ms and verification latency of 25.09 ms, significantly outperforming existing technology in privacy protection, computational efficiency, and security robustness. The research provides an innovative technical solution for cryptocurrency dual-offline payment, advancing both theoretical foundations and practical applications in the field. Full article
(This article belongs to the Special Issue Data Privacy Protection in Blockchain Systems)
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12 pages, 3085 KB  
Article
Data-Driven Interactive Lens Control System Based on Dielectric Elastomer
by Hui Zhang, Zhijie Xia, Zhisheng Zhang and Jianxiong Zhu
Technologies 2026, 14(1), 68; https://doi.org/10.3390/technologies14010068 - 16 Jan 2026
Abstract
In order to solve the dynamic analysis and interactive imaging control problems in the deformation process of bionic soft lenses, dielectric elastomer (DE) actuators are separated from a convex lens, and data-driven eye-controlled motion technology is investigated. According to the DE properties, which [...] Read more.
In order to solve the dynamic analysis and interactive imaging control problems in the deformation process of bionic soft lenses, dielectric elastomer (DE) actuators are separated from a convex lens, and data-driven eye-controlled motion technology is investigated. According to the DE properties, which are consistent with the deformation characteristics of hydrogel electrodes, the motion and deformation effect of eye-controlled lenses under film prestretching, lens size, and driving voltage, is studied. The results show that when the driving voltage increases to 7.8 kV, the focal length of the lens, whose prestretching λ is 4, and the diameter d is 1 cm, varies in the range of 49.7 mm and 112.5 mm. And the maximum focal-length change could reach 58.9%. In the process of eye controlling design and experimental verification, a high DC voltage supply was programmed, and eye movement signals for controlling the lens were analyzed by MATLAB software (R2023b). Eye-controlled interactive real-time motion and tunable imaging of the lens were realized. The response efficiency of soft lenses could reach over 93%. The adaptive lens system developed in this research has the potential to be applied to medical rehabilitation, exploration, augmented reality (AR), and virtual reality (VR) in the future. Full article
(This article belongs to the Special Issue AI Driven Sensors and Their Applications)
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38 pages, 7681 KB  
Article
A Sequential GAN–CNN–FUZZY Framework for Robust Face Recognition and Attentiveness Analysis in E-Learning
by Chaimaa Khoudda, Yassine El Harrass, Kaoutar Tazi, Salma Azzouzi and Moulay El Hassan Charaf
Appl. Sci. 2026, 16(2), 909; https://doi.org/10.3390/app16020909 - 15 Jan 2026
Viewed by 18
Abstract
In modern e-learning environments, ensuring both student identity verification and concentration monitoring during online examinations has become increasingly important. This paper introduces a robust sequential framework that integrates Generative Adversarial Networks (GANs), Convolutional Neural Networks (CNNs) and fuzzy logic to achieve reliable face [...] Read more.
In modern e-learning environments, ensuring both student identity verification and concentration monitoring during online examinations has become increasingly important. This paper introduces a robust sequential framework that integrates Generative Adversarial Networks (GANs), Convolutional Neural Networks (CNNs) and fuzzy logic to achieve reliable face recognition and interpretable attentiveness assessment. Images from the Extended Yale B (cropped) dataset are preprocessed through grayscale normalization and resizing, while GANs generate synthetic variations in pose, illumination, and occlusion to enrich the training set and improve generalization. The CNN extracts discriminative facial features for identity recognition, and a fuzzy inference system transforms the CNN’s confidence scores into human-interpretable concentration levels. To stabilize learning and prevent overfitting, the model incorporates dropout regularization, batch normalization, and extensive data augmentation. Comprehensive evaluations using confusion matrices, ROC–AUC, and precision–recall analyses demonstrate an accuracy of 98.42%. The proposed framework offers a scalable and interpretable solution for secure and reliable online exam proctoring. Full article
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27 pages, 7771 KB  
Review
Advances in Folding-Wing Flying Underwater Drone (FUD) Technology
by Jianqiu Tu, Junjie Zhuang, Haixin Chen, Changjian Zhao, Hairui Zhang and Wenbiao Gan
Drones 2026, 10(1), 62; https://doi.org/10.3390/drones10010062 - 15 Jan 2026
Viewed by 25
Abstract
The evolution of modern warfare and civil exploration requires platforms that can operate seamlessly across the air–water interface. The folding-wing Hybrid Air and Underwater Vehicle (FUD) has emerged as a transformative solution, combining the high-speed cruising capabilities of fixed-wing aircraft with the stealth [...] Read more.
The evolution of modern warfare and civil exploration requires platforms that can operate seamlessly across the air–water interface. The folding-wing Hybrid Air and Underwater Vehicle (FUD) has emerged as a transformative solution, combining the high-speed cruising capabilities of fixed-wing aircraft with the stealth characteristics of underwater navigation. This review thoroughly analyzes the advancements and challenges in folding-wing FUD technology. The discussion is framed around four interconnected pillars: the overall design driven by morphing technology, adaptation of the propulsion system, multi-phase dynamic modeling and control, and experimental verification. The paper systematically compares existing technical pathways, including lateral and longitudinal folding mechanisms, as well as dual-use and hybrid propulsion strategies. The analysis indicates that, although significant progress has been made with prototypes demonstrating the ability to transition between air and water, core challenges persist. These challenges include underwater endurance, structural reliability under impact loads, and effective integration of the power system. Additionally, this paper explores promising application scenarios in both military and civilian domains, discussing future development trends that focus on intelligence, integration, and clustering. This review not only consolidates the current state of technology but also emphasizes the necessity for interdisciplinary approaches. By combining advanced materials, computational intelligence, and robust control systems, we can overcome existing barriers to progress. In conclusion, FUD technology is moving from conceptual validation to practical engineering applications, positioning itself to become a crucial asset in future cross-domain operations. Full article
(This article belongs to the Special Issue Advances in Autonomous Underwater Drones: 2nd Edition)
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15 pages, 1607 KB  
Article
Using Steganography and Artificial Neural Network for Data Forensic Validation and Counter Image Deepfakes
by Matimu Caswell Nkuna, Ebenezer Esenogho and Ahmed Ali
Computers 2026, 15(1), 61; https://doi.org/10.3390/computers15010061 - 15 Jan 2026
Viewed by 43
Abstract
The merging of the Internet of Things (IoT) and Artificial Intelligence (AI) advances has intensified challenges related to data authenticity and security. These advancements necessitate a multi-layered security approach to ensure the security, reliability, and integrity of critical infrastructure and intelligent surveillance systems. [...] Read more.
The merging of the Internet of Things (IoT) and Artificial Intelligence (AI) advances has intensified challenges related to data authenticity and security. These advancements necessitate a multi-layered security approach to ensure the security, reliability, and integrity of critical infrastructure and intelligent surveillance systems. This paper proposes a two-layered security approach that combines a discrete cosine transform least significant bit 2 (DCT-LSB-2) with artificial neural networks (ANNs) for data forensic validation and mitigating deepfakes. The proposed model encodes validation codes within the LSBs of cover images captured by an IoT camera on the sender side, leveraging the DCT approach to enhance the resilience against steganalysis. On the receiver side, a reverse DCT-LSB-2 process decodes the embedded validation code, which is subjected to authenticity verification by a pre-trained ANN model. The ANN validates the integrity of the decoded code and ensures that only device-originated, untampered images are accepted. The proposed framework achieved an average SSIM of 0.9927 across the entire investigated embedding capacity, ranging from 0 to 1.988 bpp. DCT-LSB-2 showed a stable Peak Signal-to-Noise Ratio (average 42.44 dB) under various evaluated payloads ranging from 0 to 100 kB. The proposed model achieved a resilient and robust multi-layered data forensic validation system. Full article
(This article belongs to the Special Issue Multimedia Data and Network Security)
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22 pages, 20100 KB  
Article
Real-Time Detection and Validation of a Target-Oriented Model for Spindle-Shaped Tree Trunks Leveraging Deep Learning
by Kang Zheng, Shuo Yang, Zhichong Wang, Hao Fu, Xiu Wang, Wei Zou, Changyuan Zhai and Liping Chen
Agronomy 2026, 16(2), 210; https://doi.org/10.3390/agronomy16020210 - 15 Jan 2026
Viewed by 100
Abstract
To enhance the automation and intelligence of trenching fertilization operations, this research proposes a real-time trunk detection model (Trunk-Seek) designed for spindle-shaped orchards. The model employs a customized data augmentation strategy and integrates the YOLO deep learning framework to effectively address visual challenges [...] Read more.
To enhance the automation and intelligence of trenching fertilization operations, this research proposes a real-time trunk detection model (Trunk-Seek) designed for spindle-shaped orchards. The model employs a customized data augmentation strategy and integrates the YOLO deep learning framework to effectively address visual challenges such as lighting variation, occlusion, and motion blur. Multiple object tracking algorithms were evaluated, and ByteTrack was selected for its superior performance in dynamic trunk tracking. In addition, a Positioning and Triggering Algorithm (PTA) was developed to enable precise localization and triggering for target-oriented fertilization. The system was deployed on an edge device, a test bench was established, and both laboratory and field experiments were conducted to validate its performance. Experimental results demonstrated that the detection model achieved an mAP50 of 98.9% and maintained a stable 32.53 FPS on the edge device, fulfilling real-time detection requirements. Test bench analysis revealed that variations in trunk diameter and operation speed affected triggering accuracy, with an average dynamic localization error of ±1.78 cm. An empirical model (T) was developed to describe the time-delay behavior associated with positioning errors. Field verification in orchards confirmed that Trunk-Seek achieved a triggering accuracy of 91.08%, representing a 24.08% improvement over conventional training methods. Combining high accuracy with robust real-time performance, Trunk-Seek and the proposed PTA provide essential technical support for the development of a visual target-oriented fertilization system in modern orchards. Full article
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29 pages, 4136 KB  
Article
Intelligent Prediction Model for Icing of Asphalt Pavements in Cold Regions Oriented to Geothermal Deicing Systems
by Junming Mo, Ke Wu, Jiading Jiang, Lei Qu, Wenbin Wei and Jinfu Zhu
Processes 2026, 14(2), 294; https://doi.org/10.3390/pr14020294 - 14 Jan 2026
Viewed by 69
Abstract
To address traffic safety hazards from asphalt pavement icing in Xinjiang’s cold regions and inefficiencies of conventional deicing and imprecise geothermal deicing systems, this study focused on local asphalt surfaces. Using “outdoor qualitative screening and indoor quantitative verification”, key variables were identified via [...] Read more.
To address traffic safety hazards from asphalt pavement icing in Xinjiang’s cold regions and inefficiencies of conventional deicing and imprecise geothermal deicing systems, this study focused on local asphalt surfaces. Using “outdoor qualitative screening and indoor quantitative verification”, key variables were identified via controlled tests and their coupling effects on the time to complete icing were quantified through an L16(44) orthogonal test (a 4-factor, 4-level design encompassing 16 test groups). A Backpropagation (BP) neural network model (3 inputs, 5 hidden neurons, and a learning rate of 0.7) optimized with 64 datasets was established to predict the time to complete icing of asphalt pavements, achieving a prediction accuracy (PA) of 90.7% for the time to complete icing and a mean error of merely 0.71 min. Dynamic icing risk thresholds (high/medium/low) were established via K-means clustering and statistical tests, enabling data-driven precise activation and on-demand regulation of geothermal deicing systems. This resolves energy waste and deicing delays, offering technical support for efficient geothermal utilization in cold-region transportation infrastructure, and provides a scalable “factor screening + model prediction” framework for asphalt pavement anti-icing practice. Full article
(This article belongs to the Special Issue Innovative Technologies and Processes in Geothermal Energy Systems)
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26 pages, 5028 KB  
Article
Optimal Dispatch of Energy Storage Systems in Flexible Distribution Networks Considering Demand Response
by Yuan Xu, Zhenhua You, Yan Shi, Gang Wang, Yujue Wang and Bo Yang
Energies 2026, 19(2), 407; https://doi.org/10.3390/en19020407 - 14 Jan 2026
Viewed by 92
Abstract
With the advancement of the “dual carbon” goal, the power system is accelerating its transition towards a clean and low-carbon structure, with a continuous increase in the penetration rate of renewable energy generation (REG). However, the volatility and uncertainty of REG output pose [...] Read more.
With the advancement of the “dual carbon” goal, the power system is accelerating its transition towards a clean and low-carbon structure, with a continuous increase in the penetration rate of renewable energy generation (REG). However, the volatility and uncertainty of REG output pose severe challenges to power grid operation. Traditional distribution networks face immense pressure in terms of scheduling flexibility and power supply reliability. Active distribution networks (ADNs), by integrating energy storage systems (ESSs), soft open points (SOPs), and demand response (DR), have become key to enhancing the system’s adaptability to high-penetration renewable energy. This work proposes a DR-aware scheduling strategy for ESS-integrated flexible distribution networks, constructing a bi-level optimization model: the upper-level introduces a price-based DR mechanism, comprehensively considering net load fluctuation, user satisfaction with electricity purchase cost, and power consumption comfort; the lower-level coordinates SOP and ESS scheduling to achieve the dual goals of grid stability and economic efficiency. The non-dominated sorting genetic algorithm III (NSGA-III) is adopted to solve the model, and case verification is conducted on the standard 33-node system. The results show that the proposed method not only improves the economic efficiency of grid operation but also effectively reduces net load fluctuation (peak–valley difference decreases from 2.020 MW to 1.377 MW, a reduction of 31.8%) and enhances voltage stability (voltage deviation drops from 0.254 p.u. to 0.082 p.u., a reduction of 67.7%). This demonstrates the effectiveness of the scheduling strategy in scenarios with renewable energy integration, providing a theoretical basis for the optimal operation of ADNs. Full article
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12 pages, 2700 KB  
Proceeding Paper
A Low-Cost and Reliable IoT-Based NFT Hydroponics System Using ESP32 and MING Stack
by Tolga Demir and İhsan Çiçek
Eng. Proc. 2026, 122(1), 3; https://doi.org/10.3390/engproc2026122003 - 14 Jan 2026
Viewed by 131
Abstract
This paper presents the design and implementation of an IoT-based automation system for indoor hydroponic plant cultivation using the Nutrient Film Technique. The system employs an ESP32-based controller with multiple sensors and actuators. These enable real-time monitoring and control of pH, TDS, temperature, [...] Read more.
This paper presents the design and implementation of an IoT-based automation system for indoor hydroponic plant cultivation using the Nutrient Film Technique. The system employs an ESP32-based controller with multiple sensors and actuators. These enable real-time monitoring and control of pH, TDS, temperature, humidity, light, tank level, and flow conditions. A modular five-layer architecture was developed. It combines the MING stack, which includes MQTT communication, InfluxDB time-series storage, Node-RED flow processing, and Grafana visualization. The system also includes a Flutter-based mobile app for remote access. Key features include temperature-compensated calibration, hysteresis-based control algorithms, dual-mode operation, TLS/ACL security, and automated alarm mechanisms. These features enhance reliability and safety. Experimental results showed stable pH/TDS regulation, dependable actuator and alarm responses, and secure long-term data logging. The proposed open-source and low-cost platform is scalable. It provides a solution for small-scale producers and urban farming, bridging the gap between academic prototypes and production-grade smart agriculture systems. In comparison to related works that mainly focus on monitoring, this study advances the state of the art. It combines continuous time-series logging, secure communication, flow verification, and integrated safety mechanisms to provide a reproducible testbed for future smart agriculture research. Full article
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32 pages, 7960 KB  
Article
Quality Inspection of Automated Rebar Sleeve Connections Using Point Cloud Semantic Filtering and Geometry-Prior Segmentation
by Haidong Wang, Youyu Shi, Jingjing Guo and Dachuan Chen
Buildings 2026, 16(2), 338; https://doi.org/10.3390/buildings16020338 - 13 Jan 2026
Viewed by 68
Abstract
In reinforced concrete structures, the quality of rebar sleeve connections directly impacts the structure’s safety reserve and durability. However, quality inspection is complicated by the periodic distribution of stirrups, concrete obstruction, and noise interference, presenting challenges for assessing sleeve connection integrity. This paper [...] Read more.
In reinforced concrete structures, the quality of rebar sleeve connections directly impacts the structure’s safety reserve and durability. However, quality inspection is complicated by the periodic distribution of stirrups, concrete obstruction, and noise interference, presenting challenges for assessing sleeve connection integrity. This paper proposes a training-free, interpretable framework for automated rebar sleeve connection quality inspection, leveraging point cloud semantic filtering and geometric a priori segmentation. The method constructs a polar-cylindrical framework, employing hierarchical semantic filtering to eliminate stirrup layers. Geometric a priori instance segmentation techniques are then applied, integrating θ histograms, Kasa circle fitting, and axial bridging domain constraints to reconstruct each longitudinal rebar. Sleeve detection occurs within the rebar coordinate system via radial profile analysis of length, angular coverage, and stability tests, subsequently stratified into two layers and parameterised. Sleeve projections onto column axes calculate spacing and overlap area percentages. Experiments using 18 BIM-TLS paired datasets demonstrate that this method achieves zero residual error in stirrup detection, with sleeve parameter accuracy reaching 98.9% in TLS data and recall at 57.5%, alongside stable runtime transferability. All TLS datasets meet the quality requirements of rebar sleeve connection spacing ≥35d and percentage of overlap area ≤50%. This framework enhances on-site quality inspection efficiency and consistency, providing a viable pathway for digital verification of rebar sleeve connection quality. Full article
(This article belongs to the Special Issue Intelligence and Automation in Construction—2nd Edition)
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20 pages, 2054 KB  
Article
Writer-Independent Offline Signature Verification Using Local and Global Feature Fusion
by Hongyang Wang, Xin He, Zhonghui Wei, Zhuang Lv, Zhiya Mu, Lei Zhang and Yi Gao
Symmetry 2026, 18(1), 149; https://doi.org/10.3390/sym18010149 - 13 Jan 2026
Viewed by 148
Abstract
In offline signature verification, extracting effective features and enhancing identification accuracy remain critical challenges. Traditional feature extraction modules struggle to capture comprehensive, detailed characteristics from signatures alone and often suffer from overfitting issues. This paper introduces ResT, a novel approach that integrates the [...] Read more.
In offline signature verification, extracting effective features and enhancing identification accuracy remain critical challenges. Traditional feature extraction modules struggle to capture comprehensive, detailed characteristics from signatures alone and often suffer from overfitting issues. This paper introduces ResT, a novel approach that integrates the Residual Network (ResNet) and Transformer architectures for multi-scale feature extraction. The proposed method comprises two components: ResNet blocks and Transformer blocks, designed to extract local and global signature characteristics, respectively. Within the ResNet blocks, we integrate two modules—Spatial-to-Depth Convolution (SPD-Conv) and Spatial and Channel Reconstruction Convolution (SCConv)—to emphasize stroke-level features, thereby improving local feature extraction. For the Transformer blocks, Vision Transformer (ViT) is employed to analyze the signature’s overall shape and stroke trends, capturing global features. To evaluate performance, we implement a writer-independent (WI) verification system and conduct extensive experiments on four public datasets: GPDS, CEDAR, BHSig-Bengali, and BHSig-Hindi. Results demonstrate that the proposed ResT model effectively distinguishes genuine from forged signatures and achieves competitive performance compared to existing methods. Full article
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23 pages, 24039 KB  
Article
Multi-Region Temperature Prediction in Grain Storage: Integrating WSLP Spatial Structure with LSTM–iTransformer Hybrid Framework
by Yongqi Xu, Peiru Li, Jin Qian, Limin Shi, Hui Zhang and Bangyu Li
Electronics 2026, 15(2), 357; https://doi.org/10.3390/electronics15020357 - 13 Jan 2026
Viewed by 162
Abstract
Grain security is a fundamental guarantee for social stability and sustainable development. Accurate monitoring and prediction of overall granary temperature are essential for reducing storage losses and improving warehouse management efficiency. As an integrated system, the temperature evolution of the grain pile is [...] Read more.
Grain security is a fundamental guarantee for social stability and sustainable development. Accurate monitoring and prediction of overall granary temperature are essential for reducing storage losses and improving warehouse management efficiency. As an integrated system, the temperature evolution of the grain pile is deeply affected by its inherent physical structure and heat transfer pathways. Therefore, a multi-level warehouse–surface–line–point (WSLP) structural modeling approach driven by the physical properties of the grain pile is proposed to extract the joint environmental and spatial characteristics. Building upon the WSLP framework, a dual-channel time-series prediction architecture integrating both long short-term memory (LSTM) and iTransformer through a mutual verification fusion mechanism is developed to enable synchronized temperature forecasting across different regions of the grain piles. Experiments are conducted using real granary data from Shandong, China. The results demonstrate that the proposed model achieves more than 30% improvement over baseline methods in terms of MAE and RMSE. Moreover, the WSLP-LSTM–iTransformer framework significantly improves prediction accuracy in complex warehouse environments and enhances the interpretability and applicability of deep learning models for grain condition forecasting by incorporating real environmental characteristics. Full article
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15 pages, 5093 KB  
Article
Single-Cell Tracking of Brewing Yeast Dynamics in Baijiu Fermentation Using GFP-Labeled Engineered Saccharomyces cerevisiae FSC01
by Yeyu Huang, Jie Meng, Xinglin Han, Dan Huang, Ruiqi Luo and Deliang Wang
Fermentation 2026, 12(1), 45; https://doi.org/10.3390/fermentation12010045 - 13 Jan 2026
Viewed by 187
Abstract
In view of the technical bottleneck of microbial dynamic monitoring during the solid-state fermentation of traditional Baijiu, this study introduced green fluorescent protein (GFP) labeling technology into the dominant Saccharomyces cerevisiae of Jiang-flavored Baijiu to construct the chromosomal integration engineering strain named FSC01. [...] Read more.
In view of the technical bottleneck of microbial dynamic monitoring during the solid-state fermentation of traditional Baijiu, this study introduced green fluorescent protein (GFP) labeling technology into the dominant Saccharomyces cerevisiae of Jiang-flavored Baijiu to construct the chromosomal integration engineering strain named FSC01. By designing an integrated recombinant plasmid containing the GFP gene and the geneticmycin resistance gene, an engineered strain that stably expresses fluorescent proteins was obtained by electroconversion. Flow cytometry verification showed that FSC01 showed excellent linear responses in the pure microbial system (R2 = 0.998) and the complex matrix of Baijiu jiupei (R2 = 0.981), with a detection limit of 102 cells/mL, and the detection cycle was shortened to 10 min. Solid-state fermentation simulation experiments show that the inoculation volume of FSC01 of 105 cells/kg can not only ensure the effective identification of fluorescence signals, but also does not significantly interfere with the growth and growth patterns of the original yeast (p > 0.05), which is highly consistent with the results of the traditional plate counting method. Dynamic monitoring shows that Saccharomyces cerevisiae during fermentation presents a typical succession pattern of “increase first and then decrease”, reaching a peak on the 7th day (1.2 × 107 cells/g), which is positively correlated with the base alcohol yield rate (26.7%). Compared with metagenomic (72 h) and PMA-qPCR (4 h) methods, this technology breaks through the limitations of specificity and timeliness of live bacteria detection, and provides a single-cell-level dynamic analysis tool for the digitization of traditional brewing processes. In the future, it will be expanded to monitor key functional microorganisms such as lactic acid bacteria through a multi-color fluorescent labeling system, and optimized pretreatment to eliminate starch granule interference, and promote the in-depth application of synthetic biology technology in the traditional fermentation industry. Full article
(This article belongs to the Section Fermentation Process Design)
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17 pages, 2471 KB  
Article
Learning Curve of Cardiac Surgery Residents in Transit-Time Flow Measurement and High-Resolution Epicardial Ultrasonography During Coronary Surgery
by Federico Cammertoni, Gabriele Di Giammarco, Nicola Testa, Natalia Pavone, Alberta Marcolini, Serena D’Avino, Piergiorgio Bruno, Maria Grandinetti, Francesco Bianchini, Antonio E. Trapani and Massimo Massetti
J. Clin. Med. 2026, 15(2), 620; https://doi.org/10.3390/jcm15020620 - 13 Jan 2026
Viewed by 136
Abstract
Objectives: This study aimed to define the learning curve required for cardiac surgery residents to acquire basic technical and interpretive skills in transit-time flow measurement (TTFM) and high-resolution epicardial ultrasonography (HRUS) during coronary artery bypass grafting (CABG). Methods: Prospective, observational, single-center [...] Read more.
Objectives: This study aimed to define the learning curve required for cardiac surgery residents to acquire basic technical and interpretive skills in transit-time flow measurement (TTFM) and high-resolution epicardial ultrasonography (HRUS) during coronary artery bypass grafting (CABG). Methods: Prospective, observational, single-center study evaluating performance using a novel scoring system combining functional (TTFM) and anatomical (HRUS) assessment criteria. This study was registered on ClinicalTrials.gov (Identifier: NCT06589323). Nine cardiac surgery residents without prior hands-on experience in TTFM or HRUS were enrolled. Twenty-seven elective CABG patients (67 grafts) were analyzed. Each measurement was compared with those obtained by an expert benchmark surgeon (N.T.) under standardized hemodynamic conditions. Results: Residents achieved the predefined primary endpoint (combined TTFM + HRUS score/number of grafts ≥ 11) after a median of 3 cases (IQR 2–4) and 7 anastomoses (IQR 7–10). Kaplan–Meier analysis showed a progressive increase in the probability of success, with a sharp rise after the seventh anastomosis. A shorter interval between attempts (<30 days) was significantly associated with earlier achievement of the endpoint (p < 0.05). Median acquisition time for TTFM was 25 s, with <10% inter-observer variability across all flow parameters. HRUS images of adequate quality were obtained within 60 s in >90% of cases, though slightly lower success rates were observed for lateral and inferior wall targets. No resident- or procedure-related variable was independently associated with performance improvement. Conclusions: Mastery of basic TTFM and HRUS skills requires only a few cases and anastomoses, demonstrating a short and attainable learning curve. These findings challenge the perception of a steep learning process and support the routine use of intraoperative graft verification techniques in all CABG procedures. Full article
(This article belongs to the Section General Surgery)
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