Search Results (114)

Coastal ecosystems are increasingly threatened by climate change and anthropogenic pressures, which can disrupt microbial communities and favor the emergence of pathogenic organisms. In this study, we applied metagenomic analysis to characterize fungal communities in sediment samples from an urban mangrove subjected to environmental stress. The results revealed a fungal community with reduced richness—28% lower than expected for similar ecosystems—likely linked to physicochemical changes such as heavy metal accumulation, acidic pH, and eutrophication, all typical of urbanized coastal areas. Notably, we detected an increase in potentially pathogenic genera, including Candida, Aspergillus, and Pseudoascochyta, alongside a decrease in key saprotrophic genera such as Fusarium and Thelebolus, indicating a shift in ecological function. The fungal assemblage was dominated by the phyla Ascomycota and Basidiomycota, and despite adverse conditions, symbiotic mycorrhizal fungi remained present, suggesting partial resilience. A considerable fraction of unclassified fungal taxa also points to underexplored microbial diversity with potential ecological or health significance. Importantly, this study does not aim to compare pristine and contaminated environments, but rather to provide a sanitary alert by identifying the presence and potential proliferation of pathogenic fungi in a degraded mangrove system. These findings highlight the sensitivity of mangrove fungal communities to environmental disturbance and reinforce the value of metagenomic approaches for monitoring ecosystem health. Incorporating fungal metagenomic surveillance into environmental management strategies is essential to better understand biodiversity loss, ecological resilience, and potential public health risks in degraded coastal environments. Full article

This study investigates the effectiveness of two cybersecurity awareness interventions—phishing simulations and organized online training—in enhancing end-user resilience to phishing attacks in a Croatian university setting. Three controlled phishing simulations and one targeted instructional module were executed across several organizational departments. This study assesses behavioral responses, compromise rates, and statistical associations with demographic variables, including age, department, and educational background. Despite educational instruction yielding a marginally reduced number of compromised users, statistical analysis revealed no meaningful difference between the two methods. The third phishing simulation, executed over a pre-holiday timeframe, demonstrated a significantly elevated compromising rate, underscoring the influence of temporal and organizational context on employee alertness. These findings highlight the shortcomings of standalone awareness assessments and stress the necessity for ongoing, contextualized, and integrated cybersecurity training approaches. The findings offer practical guidance for developing more effective phishing defense strategies within organizational environments. Full article

Introduction: Chewing gum is a widespread, seemingly mundane behaviour that has been linked to diverse benefits such as improved cognitive performance, reduced stress, and enhanced alertness. While animal and human research indicate that mastication engages extensive sensorimotor networks and may also modulate higher-order cognitive and emotional processes, questions remain about the specific neural mechanisms involved. This review combines findings from neuroimaging studies—including fMRI, fNIRS, and EEG—that investigate how chewing gum alters brain activity in humans. Methods: Using a targeted search strategy, we screened the major databases (PubMed/Medline, Scopus, ResearchGate, Google Scholar, and Cochrane) from January 1980 to March 2025 for clinical studies published in English. Eligible studies explicitly measured brain activity during gum chewing using EEG, fNIRS, or fMRI. Results: After a title/abstract screening and a full-text review, thirty-two studies met the inclusion criteria for this review: 15 utilising fMRI, 10 using fNIRS, 2 using both fNIRS and EEG, and 5 employing EEG. Overall, the fMRI investigations consistently reported strong activation in bilateral motor and somatosensory cortices, the supplementary motor area, the insula, the cerebellum, and the thalamus, during gum chewing, with several studies also noting involvement of higher-order prefrontal and cingulate regions, particularly under stress conditions or when participants chewed flavoured gum. The fNIRS findings indicated that chewing gum increased oxygenated haemoglobin in the prefrontal cortex, reflecting heightened cortical blood flow; these effects were often amplified when the gum was flavoured or when participants were exposed to stressful stimuli, suggesting that both sensory and emotional variables can influence chewing-related cortical responses. Finally, the EEG studies documented transient increases in alpha and beta wave power during gum chewing, particularly when flavoured gum was used, and reported short-lived enhancements in vigilance or alertness, which tended to subside soon after participants ceased chewing. Conclusions: Neuroimaging data indicate that chewing gum reliably engages broad sensorimotor circuits while also influencing regions tied to attention, stress regulation, and possibly memory. Although these effects are often short-lived, the range of outcomes—from changes in cortical oxygenation to shifts in EEG power—underscores chewing gum’s capacity to modulate brain function beyond simple oral motor control. However, at this time, the neural changes associated with gum chewing cannot be directly linked to the positive behavioural and functional outcomes observed in studies that measure these effects without the use of neuroimaging techniques. Future research should address longer-term impacts, refine methods to isolate flavour or stress variables, and explore potential therapeutic applications for mastication-based interventions. Full article

Coastal health assessment and diagnosis are important tools for decision-making and coastal management. In this paper, the concept of ecosystem health, which uses medical terminology to define the state of coastal health, was applied to examine and diagnose the state of the physical coastal systems of 120 coastal sites along the Moroccan Mediterranean coastline. Based on this assessment approach, five categories are defined: (1) “Good Health”, with two subdivisions: (1a) “Health Warning” and (1b) “Surface Wounds”; (2) “Minor Injury”; (3) “Major Injury”; (4) “On Life Support” and (5) “Deceased”. According to the results obtained, 38 sites (32%) were classified in the “Good Health” category, with 35 sites (29%) in the “Health Warning” and 11 (9%) in the “Surface Wounds” subdivisions; 14 sites (12%) in each of the “Minor Injury” and “Major Injury” categories; no sites (0%) in the “On Life Support” category; and 8 sites (7%) in the “Deceased” category. The considerable percentage of sites in the alert and lower categories highlights the level of degradation and ongoing loss of coastal ecosystems along the Moroccan Mediterranean coast due to the significant impact of anthropogenic processes and inadequate coastal management practices, highlighting the current degradation of its physical state and its capacity to function naturally, i.e., its ability to respond to various present and future environmental changes. The results and proposals presented in this paper offer important perspectives for the governance, preservation, and management of coastal systems and are very useful in limiting and preventing the degradation of coastal systems linked to natural processes and the development of future anthropogenic activities. In addition, they stress the importance of protecting sites classified as “healthy” and restoring those classified as “alert” or “unhealthy”, using sound management strategies based on reliable scientific data. Full article

Caffeine is a weak, nonselective adenosine receptor antagonist. At low-to-moderate doses, caffeine has a stimulating effect; however, at higher doses, it can act as a depressant. It can function both as a neuroprotectant and a neurotoxin. In experimental Traumatic Brain Injury (TBI), administration of this psychoactive drug has been associated with beneficial or detrimental effects, depending on the dose, model, and timing. In a healthy brain, caffeine can enhance alertness and promote wakefulness. However, its consumption during late adolescence and early adulthood disrupts normal pruning processes in the context of repetitive moderate TBI (mTBI), leading to changes in dendritic spine morphology, resulting in neurological and behavioral impairments. Caffeine can potentially reduce TBI-associated intracranial pressure, oxidative stress, lipid peroxidation, cytotoxic edema, inflammation, and apoptosis. It can enhance alertness and reduce mental fatigue, which is critical for the cognitive rehabilitation of TBI patients. Additionally, caffeine positively affects immune cells and aids recovery post-TBI. Antagonizing adenosine receptors involved in controlling synaptic transmission, synaptic plasticity, and synapse toxicity can improve cognitive function. Conversely, studies have also shown that caffeine consumers report significantly higher somatic discomfort compared to non-consumers. This review aims to explore various studies and thoroughly examine the positive and negative roles of caffeine in TBI. Full article

Biological, physiological, and psychological stressors cause a “stress response” in our bodies. Stressors that are sensorily perceived (either acute or chronic) trigger hormonal responses from the sympathetic nervous system—the SAM and HPA axis—that effect intended organs to alert the individual. Other stressors have a direct effect on the target organ(s) of the body—e.g., physical injury and wounds, toxins, ionizing, and UV radiation. Both kinds of stressors change cell equilibrium, often leading to reactive oxygen species (ROS) accumulation and cellular damage. Among the signaling pathways involved in fighting these stressors, the c-Jun-N-terminal kinases (JNK) respond to diverse kinds of stressors. This review focuses on JNK1 and JNK2, both of which are ubiquitously present in all cell types, and attention is paid to gastrointestinal tract epithelial cells and their response—including tight junction disruption and cytoskeletal changes. We discuss the seemingly opposite roles of JNK1 and JNK2 in helping cells choose pro-survival and pro-apoptotic pathways. We examine the common features of the JNK protein structure and the possibilities of discovering JNK-isoform-specific inhibitors since, although JNK1 and JNK2 are involved in multiple diseases, including cancer, obesity, diabetes, musculoskeletal and liver disease, no cell-specific or isoform-specific inhibitors are available. Full article

Introduction: Previous neuroimaging studies have reported structural brain alterations and local network hyperexcitability in terms of increased slow-wave electroencephalography (EEG) activity in patients with borderline personality disorder (BPD). In particular, intermittent rhythmic delta and theta activity (IRDA/IRTA) has drawn attention in mental health contexts due to its links with metabolic imbalances, neuronal stress, and emotional dysregulation—processes that are highly pertinent to BPD. These functional disturbances may be reflected in corresponding structural brain changes. The current study investigated cortical thickness and subcortical volumes in BPD and examined their associations with IRDA/IRTA events per minute, symptom severity, and neuropsychological measures. Methods: Seventy female BPD patients and 36 age-matched female healthy controls (HC) were included (for clinical EEG comparisons even 72 patients were available). IRDA/IRTA rates were assessed using an automatic independent component analyses (ICA) approach. T1-weighted MRI data were obtained using a MAGNETOM Prisma 3T system and analyzed with FreeSurfer (version 7.2) for subcortical structures and CAT12 for cortical thickness and global volume measurements. Psychometric assessments included questionnaires such as Borderline Symptom List (BSL-23) and Inventory of Personality Organization (IPO). Neuropsychological performance was evaluated with the Test for Attentional Performance (TAP), Culture Fair Intelligence Test (CFT-20-R), and Verbal Learning and Memory Test (VLMT). Results: Between-group comparisons exhibited no significant increase in IRDA/IRTA rates or structural abnormalities between the BPD and HC group. However, within the BPD group, cortical thickness of the right isthmus of the cingulate gyrus negatively correlated with the IRDA/IRTA difference (after minus before hyperventilation, HV; p < 0.001). Furthermore, BPD symptom severity (BSL-23) and IPO scores positively correlated with the thickness of the right rostral anterior cingulate cortex (p < 0.001), and IPO scores were associated with the thickness of the right temporal pole (p < 0.001). Intrinsic alertness (TAP) significantly correlated with relative cerebellar volume (p = 0.01). Discussion: While no group-level structural abnormalities were observed, correlations between EEG slowing, BPD symptom severity, and alertness with cortical thickness and/or subcortical volumes suggest a potential role of the anterior cingulate cortex, temporal pole, and cerebellum in emotion regulation and cognitive functioning in BPD. Future research employing multimodal EEG-MRI approaches may provide deeper insights into the neural mechanisms underlying BPD and guide personalized therapeutic strategies. Full article

(1) Background: Human exposure to air pollution may induce inflammation and oxidative stress. In addition, extreme air temperatures and relative humidity cause vasoconstriction and abnormal blood cell function. These harmful effects may increase cardiovascular disease mortality. The effects of air pollution and climatic factors on mortality in patients with acute myocardial infarction (AMI) are relatively unknown. (2) Methods: We used AMI mortality data collected from Taiwan’s Medical Quality Indicator. Air pollutant data were collected from the Taiwanese Environmental Protection Administration, and air temperature and relative humidity data were obtained from the Taiwanese Central Weather Administration. The effects were estimated using Poisson regression to analyze the relative risk (RR) of mortality from AMI associated with exposure to air pollutants and climatic factors. (3) Results: The RR for every 4.7 μg/m³ increase in particulate matter with a diameter less than 2.5 μm (PM_2.5) was 1.086 (95% CI: 1.033–1.142, p = 0.001). The RR for every 10.3 ppb increase in ozone concentration was 1.095 (95% CI: 1.011–1.185, p = 0.025). The RR for every 6.5 °C decrease in air temperature was 1.087 (95% CI: 1.024–1.154, p = 0.006) for AMI mortality. (4) Conclusions: This study revealed that higher PM_2.5 and ozone concentrations, along with cold weather, are associated with mortality in individuals with AMI. The government must develop policies to promote air pollution prevention, mitigate air pollution, and alert residents about poor air quality and cold weather, thereby promoting sustainable human health. Full article

Drowsiness and stress greatly influence worker health and productivity and workplace safety. Conflict between workplace expectations and employee control results in stress, which causes mental and physical reactions that affect performance and raise the risk of accidents at work. A common precursor to inadvertent drowsiness increases workplace risks and costs due to lost productivity and accidents. Developments in the interdisciplinary subject of neuroergonomics enable the creation of novel systems to track and minimize these issues. This work introduces prototype testing to demonstrate the system’s ability to detect stress and drowsiness. Along with other indicators such as body temperature, heart rate (HR), and SpO₂ levels, the system incorporates electroencephalography (EEG) and heart rate variability (HRV). By analyzing these biosignals, the system detects stress and drowsiness in real time, providing alerts to both users and the supervisor’s BI dashboard. The design is flexible, offering two wearable forms: a headband and an armband. Prototype testing demonstrates the system’s ability to detect stress and drowsiness effectively, paving the way for safer and more productive workplace environments. Full article

Congenital heart diseases (CHDs) lead to psychological and social repercussions for parents of affected children: the diagnosis, screenings, surgeries, and hospitalization, as well as ongoing difficulties bring with them stress, anxiety, fear, stigmatization, and isolation. Studies investigating parents’ direct perspective on these issues lack in the field literature. Our research aims to leverage parents’ narratives in order to explore how they describe their role as parents of a child with CHD and the impact of its social and clinical repercussions on their lives. We recruited 45 parents and analyzed their narratives through the MADIT approach, focusing on the discursive modalities and content cores employed. Parents describe and judge their role as ‘worried-protective’, ‘heroic’, ‘normal-untroubled’, and ‘unfortunate’, in a way that strongly characterizes the person, leaving limited possibilities for assuming different features. The clusters ‘state of ordeal’, ‘state of alert-overprotection’, and ‘personal identity changes’ are connoted as inevitable and established component of parents’ lives, while ‘limitation of life experiences’ is less monolithic and more open to change. Current narratives assume a totalizing form in the life of these parents, that can lead to stigma and exacerbate the already present difficulties and challenges, that need targeted psychological intervention by field professionals. Full article

Khat, derived from the Catha edulis plant, is widely consumed in East Africa and the Arabian Peninsula, where it holds significant socio-cultural importance. This review examines the multifaceted effects of khat, particularly focusing on its cardiovascular implications. Khat’s active constituents, notably cathinone and cathine, exhibit stimulant and psychoactive properties akin to amphetamines, leading to heightened alertness and euphoria. However, chronic consumption is associated with adverse effects, including cardiovascular diseases such as hypertension, myocardial infarction, and cardiomyopathy. The review highlights the pharmacokinetics of khat, with cathinone being rapidly absorbed and leading to sympathomimetic effects. Studies indicate a correlation between chronic khat use and increased risks of hypertension, acute coronary syndromes, and cardiomyopathy. These cardiovascular conditions are exacerbated by prolonged hemodynamic stress, catecholamine release, and oxidative stress induced by khat’s active compounds. Additionally, khat’s impact extends beyond the cardiovascular system, affecting neurological, reproductive, and gastrointestinal health. Despite its legality in certain regions, khat is classified as a controlled substance in many countries, emphasizing the need for global awareness of its health risks. This review calls for longitudinal studies to elucidate the pathophysiological mechanisms of khat-induced cardiomyopathy and to identify potential biomarkers for its early detection. Furthermore, it advocates for culturally sensitive public health initiatives and clinical guidelines to mitigate the adverse health effects of khat consumption, especially among immigrant populations in developed nations. Recognizing and addressing khat’s cardiovascular implications is crucial for improving patient outcomes and guiding effective clinical practice. Full article

This study presents a framework for integrating digital twins and knowledge graphs to enhance heritage building conservation, addressing challenges in environmental stress management, material degradation, and structural integrity while preserving historical authenticity. Using validated synthetic data, the framework enables real-time monitoring, predictive maintenance, and emergency response through a digital twin connected to a knowledge graph. Four scenarios were simulated to evaluate system performance: high humidity exceeding a 75% threshold triggered alerts for limestone maintenance; temperature fluctuations caused strain levels up to 0.009 units in load-bearing components at 35 °C, necessitating structural inspection; cumulative degradation monitoring projected re-plastering needs by month eight as the plaster degradation index approached 85%; and sudden impact events simulated emergency responses, with strain spikes over 0.004 units prompting real-time alerts within 2.5 s. Response times averaged 50 ms under normal conditions, peaking at 150 ms during high-frequency updates, showing robust Application Programming Interface (API) performance and data synchronization. SPARQL (SPARQL Protocol and RDF Query Language) queries within the knowledge graph facilitated proactive maintenance scheduling, reducing reactive interventions and supporting sustainable heritage conservation, especially suited to humid–temperate climates. This framework offers a novel, structured approach that bridges modern technology with heritage preservation needs, addressing both urgent conservation challenges and long-term sustainability to ensure the resilience of heritage buildings. Full article

Background/Objectives: Obesity is influenced by biological, hormonal, and social factors, contributing to chronic diseases and burdening the healthcare system. Chronic stress and emotional eating are linked to weight gain, affecting eating behaviors and metabolism. This study aimed to assess the association between stress, eating behavior, and adiposity in obese women. Methods: This cross-sectional study included 132 obese women from Viçosa, Minas Gerais, Brazil. The participants completed the Lipp Stress Symptoms Inventory and the Dutch Eating Behavior Questionnaire. Blood samples were collected to measure plasma cortisol, and Body Mass Index (BMI) was calculated from weight and height measurements. Body fat was assessed using dual-energy X-ray absorptiometry (DXA). Associations between stress, eating behavior, and adiposity were evaluated using linear regression models, and interactions between stress and eating behavior subscales were tested. Results: Positive associations were observed between Phases I (alert), II (resistance), and III (exhaustion) of stress with emotional and external eating. A negative association was identified between dietary restraint and body fat, especially in women with lower cortisol levels (<13.7 mg/dL). Additionally, the alert phase was associated with higher android fat in these women. Conclusions: The findings support the hypothesis that stress and eating behavior are associated with body adiposity and that stress is linked to emotional and external eating. An inverse association between restrained eating and body fat was observed in women with lower cortisol levels. These results highlight the importance of an interdisciplinary approach that incorporates emotional and stress conditions in obesity treatment. Full article

With the rapid rise in technological advancements, health can be tracked and monitored in multiple ways. Tracking and monitoring healthcare gives the option to give precise interventions to people, enabling them to focus more on healthier lifestyles by minimising health issues concerning long screen time. Artificial Intelligence (AI) techniques like the Large Language Model (LLM) technology enable intelligent smart assistants to be used on mobile devices and in other cases. The proposed system uses the power of IoT and LLMs to create a virtual personal assistant for long-time screen users by monitoring their health parameters, with various sensors for the real-time monitoring of seating posture, heartbeat, stress levels, and the motion tracking of eye movements, etc., to constantly track, give necessary advice, and make sure that their vitals are as expected and within the safety parameters. The intelligent system combines the power of AI and Natural Language Processing (NLP) to build a virtual assistant embedded into the screens of mobile devices, laptops, desktops, and other screen devices, which employees across various workspaces use. The intelligent screen, with the integration of multiple sensors, tracks and monitors the users’ vitals along with various other necessary health parameters, and alerts them to take breaks, have water, and refresh, ensuring that the users stay healthy while using the system for work. These systems also suggest necessary exercises for the eyes, head, and other body parts. The proposed smart system is supported by user recognition to identify the current user and suggest advisory actions accordingly. The system also adapts and ensures that the users enjoy proper relaxation and focus when using the system, providing a flexible and personalised experience. The intelligent screen system monitors and improves the health of employees who have to work for a long time, thereby enhancing the productivity and concentration of employees in various organisations. Full article

Background: Dark anaerobiosis promotes the acidification of the thylakoid lumen and a reduction in the plastoquinone (PQ) pool. The relationship between the reduction in the PQ pool in the dark and the induction of the xanthophyll cycle under high light stress was investigated in Chlamydomonas reinhardtii. Methods: To achieve a comprehensive oxidative/reductive (aerobic/anaerobic conditions) state of the PQ pool, cultures were bubbled with air or nitrogen for 4 h. To induce the xanthophyll cycle, the cultures were then irradiated with 1200 µmol_photons m⁻² s⁻¹ white light for 1 h. Results: The anaerobic cultures exhibited a stronger induction of the xanthophyll cycle with a 3.4-fold higher de-epoxidation state than the aerobic cultures. Chlorophyll fluorescence measurements showed that this response was influenced by the previous redox state of the PQ pool, and that dark anaerobiosis triggers physiological responses, such as exposure to high light. Thus, the photosynthetic apparatus in anaerobic cultures was already alerted, at the moment of high light exposure, to give an appropriate response to the stress with a stronger induction of the xanthophyll cycle than in aerobic cultures. Conclusions: Our results provide new information on the importance of the redox signaling pathway and highlight the importance of the reductive conditions of the PQ pool in regulating the physiological responses of photosynthetic organisms to stress. Full article