Search Results (7,614)

In the big data era, understanding and influencing consumer behavior in digital marketing increasingly relies on large-scale data and AI-driven analytics. This narrative, concept-driven review examines how big data technologies and machine learning reshape consumer behavior analysis across key decision-making areas. After outlining the theoretical foundations of consumer behavior in digital settings and the main data and AI capabilities available to marketers, this paper discusses five application domains: personalized marketing and recommender systems, dynamic pricing, customer relationship management, data-driven product development and fraud detection. For each domain, it highlights how algorithmic models affect targeting, prediction, consumer experience and perceived fairness. This review then turns to synthetic data as a privacy-oriented way to support model development, experimentation and scenario analysis, and to dark data as a largely underused source of behavioral insight in the form of logs, service interactions and other unstructured records. A discussion section integrates these strands, outlines implications for digital marketing practice and identifies research needs related to validation, governance and consumer trust. Finally, this paper sketches future directions, including deeper integration of AI in real-time decision systems, increased use of edge computing, stronger consumer participation in data use, clearer ethical frameworks and exploratory work on quantum methods. Full article

In this study, a phase change material (PCM) in the form of technical-grade octadecane and oriented strand boards (OSBs), which are boards made from wood strands, are used to develop a latent heat storage board with the aim of utilizing this material in building construction while lowering energy consumption. The incorporation of PCM into buildings is difficult for several reasons, including the organic phase material’s flammability and leakage during phase change. These obstacles have been overcome to a significant extent in the engineered OSB material. To avoid PCM from leaking throughout the phase change regime, PCM was hosted in the oriented strand board (OSB) using high-density polyethylene to develop a shape-stabilized phase change wood-based board (SSPCM-OSB). To improve the binding between PCM and OSBs and reduce the flammability, additional additives were added. Extensive testing was conducted to determine the physical and thermal properties and heat transfer characteristics of the developed SSPCM-OSB. The newly developed oriented strand board with SSPCM integration has a lower heat flux than a conventional OSB and comparable flammability characteristics. Full article

Escherichia coli is a major pathogen responsible for urinary tract infections, septicemia, and other clinically relevant conditions, with increasing multidrug resistance (MDR) limiting available treatment options. In this context, bacteriophages represent a valuable resource for exploring novel antimicrobial and biotechnological tools. Here, we report the isolation and genomic characterization of BorMax, a novel lytic phage infecting multiple MDR E. coli. Transmission electron microscopy revealed a tailed morphology consistent with Dhillonvirus. Whole genome sequencing and de novo assembly showed a linear double-stranded DNA genome of 45,502 bp, encoding 70 predicted coding sequences (CDSs) and lacking tRNAs. Bioinformatic analyses confirmed the absence of lysogeny-associated genes, as well as virulence and antimicrobial resistance determinants. Comparative genomics using classified BorMax within the genus Dhillonvirus as a new species, sharing <77% intergenomic similarity with known members. Notably, predictions using PaCRISPR and AcRanker identified four CDSs with strong anti-CRISPR (Acr) potential, representing previously undescribed Acr candidates in this group. These genomic features highlight the novelty, safety, and potential biotechnological relevance of BorMax and contribute to the expanding genomic and functional diversity of Dhillonvirus and E. coli-infecting phages. Full article

Synthetic RNA has become an essential modality in therapeutic development. Linear mRNA is already clinically validated, which demonstrated that in vitro-transcribed (IVT) RNA can achieve robust protein expression in humans and can be manufactured at a large scale. Circular RNA (circRNA) represents a more recent format characterized by a covalently closed backbone that confers enhanced resistance to exonucleases and supports sustained translation when paired with appropriate regulatory elements. Although both formats are produced through cell-free synthesis, their manufacturing pathways are distinct. Linear mRNA synthesis requires transcription, capping, polyadenylation, and stringent removal of double-stranded RNA contaminants. circRNA production generally proceeds through transcription of a linear precursor followed by enzymatic or ribozyme-mediated circularization, with emerging strategies such as permuted intron-exon designs improving efficiency and reducing extraneous sequence content. This review summarizes the principal methods used to generate linear and circRNA and identifies the technical barriers that must be overcome during the manufacturing process. Full article

Fusion-associated small transmembrane (FAST) proteins are viral nonstructural proteins known to be encoded by specific members of the Spinareoviridae, specifically within the Aquareovirus and Orthoreovirus genera. These proteins specialize in mediating cell–cell fusion, leading to syncytia. Unlike enveloped viruses, naked viruses do not rely on fusion proteins for cell entry; however, such proteins may facilitate viral spread between cells. Although not essential for virus replication, FAST proteins have been shown to enhance viral replication, particularly during the early stages of infection. More recently, proteins with characteristics resembling FAST proteins have been identified in a broader range of viruses, including several rotavirus species within the family Sedoreoviridae, and, unexpectedly, in some enveloped viruses within the Coronaviridae family. Here, we present protein sequence analyses suggesting that viruses of the recently established virus family Pistolviridae (order Ghabrivirales) also encode proteins with similarity to FAST proteins. Pistolviruses are small double-stranded RNA viruses that infect piscine species, and were initially referred to as “toti-like” viruses due to genomic similarities with members of the former Totiviridae, which infect single-celled organisms. The putative FAST proteins of the pistolviruses may be expressed either from small, distinct open reading frames or suggested to be produced as cleavage products derived from polyproteins. Full article

This exploratory mixed-methods study examined whether a narrative-driven digital gamification platform, FantasyClass, grounded in the MDA (Mechanics–Dynamics–Aesthetics) framework and Bartle’s player typology (used as a cohort-level design input), was associated with science attitudinal change in preservice primary teachers. The quantitative component employed a one-group pretest–posttest (pre-experimental) within-participant design using a validated 22-item attitudes questionnaire (N = 23), structured across three temporal dimensions: past (retrospective experiences), present (current perceptions), and future (teaching expectations). Significant improvements were observed across all attitudinal dimensions with large effect sizes, most notably in students’ future expectations and confidence to teach science. Exploratory correlation analyses indicated that participants’ perceived motivational value of narrative and immersion elements was moderately associated with Future-dimension attitudinal gains. Qualitative thematic analysis of open-ended responses (n = 15) revealed enhanced motivation, reduced science anxiety, more positive perceptions of physics and chemistry, and strong intentions to adopt game-based and gamified strategies in future teaching practice. Convergence across quantitative and qualitative strands suggests that structurally coherent, player-type-informed narrative gamification may be associated with attitudinal transformation and early professional identity development in STEM teacher education, while recognizing that the design does not permit causal attribution. Full article

The Atlantic bluefin tuna (Thunnus thynnus) is a high-value species subject to strict catch quotas and seasonal closures in the Mediterranean Sea. However, detecting illegal, unreported, and unregulated fishing remains challenging, particularly for small-scale activities. The aim of this study is to investigate whether citizen-reported strandings of tuna remains along Italian coasts can provide potential indirect indications of illegal fishing activities. To address this question, we collected and verified photographic records of stranded tuna remains (e.g., skulls, vertebral elements, complete specimens) reported on social networks. A similar search was conducted for two other conspicuous fishes, the ‘dusky grouper’ (Epinephelus marginatus) and the ‘greater amberjack’ (Seriola dumerili), as controls. Thirty-two strandings of tuna remains were recorded, with no records of amberjacks and only one of dusky grouper. Most strandings involved tuna heads or neurocrania, some of which showed clear mechanical cuts indicating post-capture processing. Several remains were older and degraded, suggesting long-term persistence in the sea. Overall, our results indicate that citizen-reported strandings could provide low-cost, spatially broad potential indications of discards resulting from illegal fishing activities. Wider adoption of standardised public reporting could not only engage the public in marine conservation, but also provide valuable data for policymakers tackling illegal fishing and complement traditional fisheries monitoring. Full article

Pleurotus ostreatus, a globally cultivated oyster mushroom, is susceptible to viral infections that threaten yield and quality. This study reports the identification and characterization of three novel viruses from a symptomatic P. ostreatus strain K3: Pleurotus ostreatus deltaflexivirus 2, 3, and 4 (PoDFV2, PoDFV3, PoDFV4). Complete genome sequencing revealed that they are single-stranded, positive-sense RNA viruses with lengths of 7809 nt, 7771 nt, and 7786 nt, encoding 5, 2, and 4 open reading frames (ORFs), respectively. The largest open reading frame (ORF1) encodes a putative replication-associated polyprotein (RP) containing three conserved domains—viral RNA methyltransferase (Mtr), viral RNA helicase (Hel), and RNA-dependent RNA polymerase (RdRp). Based on genomic sequence analysis, multiple sequence alignments, and phylogenetic analysis, PoDFV2–4 were identified as novel viruses of the genus Deltaflexivirus within the family Deltaflexiviridae. PoDFV2–4 had no significant effects on mycelial growth rate, plate mycelial biomass, or laccase activity. However, they significantly inhibited mycelial cellulase activity and resulted in malformed fruiting bodies, as well as a substantial reduction in yield. Full article

The southeastern Iberian Peninsula, particularly the Águilas Arc within the Neogene Volcanic Province (NVP), represents a promising geothermal domain with complex tectonics and geology. The Palomares Fault Zone (PFZ), a key shear structure initiated during the Late Miocene, acts as a conduit for fluid migration, promoting mineralization and potential anomalies of rare and critical metals through fluid–rock interaction. This study investigates such interactions in the southernmost Águilas Arc, focusing on the El Arteal fault segment within the eastern PFZ strand. Mineralogical, geochemical, and hydrogeological analyses were performed using XRD, SEM, and ICP-MS techniques. Results reveal six mineral assemblages (MA) within the fault segment where the fault gouge samples were characterized by cataclastic textures and the occurrence of authigenic minerals, including halite, kaolinite, illite, paragonite, goethite, hematite, gypsum, barite, celestine, and quartz. Geochemical data indicate enrichment signatures in large-ion lithophile elements (LILE) and minor chalcophile and light rare-earth elements (LREE). Two thermal hydrofacies with alkaline metals enrichment were identified in wells and mine shafts: (1) Na⁺SO₄²⁻ and (2) Na⁺Cl⁻, where the latter exhibits high Na⁺ and Cl⁻ concentrations toward deeper sectors. These findings suggest multiple stages of fluid–rock interaction controlled by temperature: an early phase dominated by epithermal mineralization, followed by late-stage circulation of hypersaline fluids. This evolution provides an abnormal geochemical signature that is unique in the Aguilas Arc Geothermal System. Full article

This study systematically investigated the genomic alterations in Saccharomyces cerevisiae driven by Replication Factor A (RFA) dosage insufficiency using a promoter-replacement strategy combined with mutation accumulation and whole-genome sequencing. Our findings reveal that transcriptional suppression of RFA2 or RFA3 leads to severe growth inhibition. RFA deficiency induces a distinct mutational spectrum characterized by a high frequency of monosomy and terminal deletions, indicative of severe replication stress. Furthermore, loss of heterozygosity is significantly enriched at centromeres and high-GC regions, underscoring the role of RFA in stabilizing intrinsic genomic barriers. Utilizing an APOBEC3B-induced mutagenesis assay, we demonstrate that RFA insufficiency leads to the extensive accumulation of exposed ssDNA with a distinct bias towards the lagging strand template. Notably, we observed that cells spontaneously inactivate Mismatch Repair (MMR) genes, such as MSH2 and PMS1, to survive RFA-induced stress. This hypermutant phenotype grants a certain degree of growth recovery on Low Galactose (LG) medium. Overall, these findings demonstrate that RFA dosage is a key determinant of genomic integrity and elucidate how repair pathway modulation drives adaptive evolution under replication stress. Full article

Background/Objectives: Nusinersen is a synthetic antisense RNA oligonucleotide employed in the management of spinal muscular atrophy, a rare neuromuscular disorder, by modulating the alternative splicing of the survival motor neuron 2 (SMN2) gene. GNR-100 represents the first generic version of the reference listed drug (RLD), containing nusinersen sodium as the active pharmaceutical ingredient. We performed comprehensive evaluations in accordance with FDA guidelines, including side-by-side comparative analyses of critical quality attributes, to thoroughly characterize the structural and functional properties of both nusinersen products. Results/Methods: GNR-100 was comprehensively demonstrated to be highly similar to RLD in terms of oligonucleotide structure, physicochemical properties, impurity profile, and in vitro cell-based assays for SMN-gene splice-switching and SMN-protein activity. Structural analyses confirmed that the oligonucleotide primary sequences and chemical structures were identical. The diastereomeric composition and higher-order structures were also similar between the proposed generic and the reference product. Comparable resistance to phosphodiesterase degradation and nearly identical melting temperatures of the oligonucleotide duplexes with their complementary strand further substantiated the structural sameness of the nusinersen products. The impurity profile of the proposed therapeutic oligonucleotide was consistent with that of RLD, and the collectively reduced levels of impurities, as assessed by orthogonal analytical methods, indicated no meaningful impact on the safety profile. Moreover, both products exhibited comparable biological activity in enhancing the production of full-length SMN2 mRNA transcripts and functional SMN protein in fibroblasts derived from SMA patients. Conclusions: These quality studies demonstrate that GNR-100 exhibits no significant differences from the licensed drug across structural, physicochemical, biophysical, and biological attributes, establishing its potential as a cost-effective therapeutic alternative for patients with spinal muscular atrophy. Full article

The use of antiretroviral therapy (ART) as the only effective way to control human immunodeficiency virus (HIV) infection results in HIV drug resistance. Next-generation sequencing (NGS) has become a common method for identifying drug-resistant variants and reducing analysis costs. The aim of this study was to develop an NGS-based protocol for identifying resistance mutations and cell tropism of HIV-1 in adult patients with and without treatment experience in Russia in 2024–2025. Plasma samples from adult HIV-infected patients from Russia were analyzed. Consensus nucleotide sequences of pol and env genes were obtained using NGS. HIV-1 drug resistance analysis was conducted using the Stanford University HIVdb database. CXCR4 cell tropism was predicted using an empirical rule classifier. A protocol for NGS of HIV-1 pol and env genes was developed. The most common HIV-1 surveillance mutations were in the reverse transcriptase. High levels of resistance were observed to non-nucleoside reverse transcriptase inhibitors (NNRTIs) and nucleoside reverse transcriptase inhibitors (NRTIs) in treatment-experienced patients and to NNRTIs in treatment-naïve patients. Low levels of resistance were observed to protease and integrase strand transfer inhibitors (INSTIs). CXCR4 cell tropism was extremely rare. NGS allows for the simultaneous processing of large data sets during epidemiological studies. The introduction of NGS-based protocols allows for performing ART efficiency and tropism monitoring at scale. Full article

The ageing of elevator travelling cables results in the breakage of inner copper strands, leading to communication and control faults in the elevator system. In this paper, a travelling cable state evaluation method based on time-frequency transformation and a deep learning fitting method is proposed. The cable diagnosis is based on the transmission line theory and finite element simulation results, which indicate that the number of broken strands of copper wires in twisted cables is positively related to the amplitude of fluctuation in the cable’s transmission spectrum. To evaluate this fluctuation with low cost and high accuracy, we acquired the 500 Msps time-domain signal after a square wave with different periods was transmitted through the detected cable; the transmission in base frequency and harmonics is calculated and combined into the total transmission spectrum. A deep learning model with a two-layer 1-D CNN and squeeze-excitation channel attention is utilized to fit the spectrum data, and cross-entropy is applied to estimate the departure between the fitting results and the experimental data, which serves as the cable’s broken-state index. Experiments demonstrate that the proposed method is able to detect minor cable faults such as one or two copper strands broken and could distinguish different broken states with a sensitivity of 16.42 ± 1.39 per break strand. Full article

The truss transfer story serves as a critical structural zone connecting different structural systems in high-rise buildings. This component incorporates numerous inclined-web trusses, which are prone to cracking and failure under seismic events. To enhance the seismic performance of long-span transfer structures and address the tensile cracking vulnerability of inclined-web trusses in conventional truss transfer stories, this study investigates the seismic behavior of a novel composite system: a prestressed CFRP tendon–steel-reinforced concrete transfer story structure with inclined-web trusses and two specimens of inclined-web truss transfer story frames—with and without prestressed CFRP tendons—were designed and fabricated. These specimens were subjected to horizontal low-cycle reversed loading to examine seismic performance indicators, including crack propagation patterns, failure modes, hysteretic curves, skeleton curves, stiffness degradation, ductility, and energy dissipation capacity. The results demonstrate that incorporating prestressed CFRP tendons into the inclined-web trusses did not alter the failure mode of the steel-reinforced concrete transfer story structure. The primary failure morphology consistently manifested as flexural-shear failure in the bottom chord columns. During the loading process, tensile cracking failure manifested in the inclined-web members of both specimens, with and without prestressing. Crack distribution remained uniform in all cases. The inclined-web trusses incorporating prestressed strands exhibited an 80% increase in cracking load compared to the non-prestressed specimen. Furthermore, the prestressed specimen demonstrated superior resistance to performance degradation and enhanced energy dissipation capacity. Both configurations exhibited significant deformation capacity and satisfactory seismic performance. The prestressed CFRP tendons enhance the crack resistance and deformation capacity of a transfer story structure with inclined-web trusses, providing novel insights for seismic design of truss transfer story structures. Full article

Genomic instability caused by defective DNA double-strand break (DSB) repair is a key determinant of cellular radiosensitivity. The Long–Evans cinnamon (LEC) rat is a rare naturally occurring model with marked radiosensitivity, and a major quantitative trait locus, X-ray hypersensitivity 1 (xhs1), has been mapped to rat chromosome 4; however, the causal mechanism has remained unclear. Here, we investigated the cellular and molecular basis of xhs1-associated radiosensitivity using LEA and LEC rat-derived cells and human cultured cells. Exploratory RNA-seq of pre-hepatitic liver tissue identified a sequence variant within the Hmces transcript in LEC rats. Consistently, HMCES protein levels were markedly reduced in multiple tissues and liver-derived cell lines from LEC rats. Functional analyses showed that reduced HMCES activity prolonged γH2AX signaling after X-ray irradiation, indicating delayed DSB resolution. Clonogenic survival assays demonstrated increased radiosensitivity in HMCES-deficient cells, which was partially rescued by restoring HMCES expression in stable LEA/LEC lines. Moreover, pimEJ5GFP reporter assays revealed significantly decreased end-joining repair activity in HMCES-knockout human cells. Together, these results establish HMCES as a critical mediator of DSB repair and cellular radioresistance, identify HMCES dysfunction as a core genetic determinant underlying xhs1-associated radiosensitivity, and provide mechanistic insight into radiation response architecture in a naturally occurring radiosensitive model. Full article