Search Results (13,084)

Mercury (Hg) bioaccumulation in freshwater fish represents a major pathway of human exposure, particularly in cascade reservoir systems where hydrological retention and legacy contamination can enhance methylmercury (MeHg) formation and trophic transfer. This study quantified total mercury (THg) concentrations in seven tissues of seven fish species from the Arda River cascade (Bulgaria). Multi-tissue measurements were integrated with morphometric predictors, multivariate statistical analyses, and combined deterministic and probabilistic human-health risk assessments. Muscle and liver contained the highest THg concentrations, whereas gills and gonads exhibited the lowest levels. Predatory species and larger individuals accumulated significantly more Hg, reflecting trophic magnification and size-dependent exposure. A longitudinal gradient across the cascade reservoirs suggests hydrological retention effects influencing mercury distribution. Species- and tissue-specific size–Hg relationships further indicate heterogeneous bioaccumulation dynamics among taxa. Risk assessment indicated acceptable exposure for adults and pregnant women at average consumption (140 g·week⁻¹), but elevated exposure for children consuming high-Hg predators. Monte Carlo simulations (N = 30,000) revealed upper-tail risks, while Safe Weekly Intake thresholds provided species-specific consumption limits. These findings highlight the value of integrating multi-tissue monitoring with probabilistic risk modelling to support evidence-based fish-consumption advisories in contaminated freshwater systems. Full article

This study comprehensively characterised the volatile composition of hydrolates obtained as by-products of the hydrodistillation of orange, mandarin, and clementine peels. Enzymatic pretreatments using pectinase, cellulase, xylanase, or their mixture were applied in purified water or citrate buffer (pH 5) prior to Clevenger hydrodistillation, and volatile profiles were analysed by HS–SPME GC–MS. Across all citrus species, hydrolates were dominated by oxygenated monoterpenes and alcohols, with α-terpineol and linalool identified as the principal constituents. Statistical analysis suggested differences in hydrolate volatile composition following enzymatic pretreatment in citrate buffer with cellulase, xylanase, or their combination (p < 0.05); notably, α-terpineol content in mandarin hydrolates nearly doubled after these treatments. Enzyme-free reflux extraction in water also led to observable changes in volatile profiles (p < 0.05), highlighting the importance of including both untreated and enzyme-free controls when evaluating enzymatic effects. The study also illustrates the distinct distribution of dominant volatiles between hydrolates with prevailing α-terpineol. These findings demonstrate the potential of enzymatic hydrodistillation for the valorisation of citrus peel by-products by enabling modulation of hydrolate volatile composition and supporting more sustainable use of citrus processing residues. Full article

Background/Objectives: Accurate determination of active pharmaceutical ingredient (API) particle size within dry powder inhaler (DPI) formulations is essential for ensuring effective pulmonary delivery but remains analytically challenging due to low API content and micronized particle size. Methods: In this study, scanning electron microscopy (SEM) coupled with energy-dispersive X-ray microanalysis (EDX) was used to directly identify and calculate the API particle size within several different commercial DPI products fit for purpose under regulatory constraints. The method exploits unique elemental markers inherent to each API, enabling reliable discrimination from excipients without prior sample modification or API extraction. Results: Large-area SEM–EDX mapping was used to localize API particles, followed by high-magnification imaging and confirmatory spot microanalysis. Particle sizes were manually measured for at least 50 API particles per formulation using image analysis software, and particle size distribution parameters were calculated from equivalent spherical diameters. Conclusions: The methodology was successfully applied to Spiriva^®, Anoro^® Ellipta, and Relvar^® Ellipta inhalation powders, revealing micronized APIs with distinct morphological features and verifying systematic application across products. Cross-validation against laser diffraction measurements of pure APIs demonstrated statistical equivalence, confirming the robustness and analytical utility of the proposed method for particle size assessment in DPI formulations. Full article

Background and Objectives: Accurate assessment of liver fibrosis is essential for treatment decisions in patients with chronic hepatitis B (CHB). Although liver biopsy is considered the reference standard, its invasive nature limits routine use. Serum-based non-invasive fibrosis scores have been proposed as alternatives; however, their diagnostic performance in CHB remains variable. This study aimed to compare multiple serum-based non-invasive fibrosis scores with liver biopsy findings and to evaluate their association with histological activity. Materials and Methods: This retrospective cross-sectional study included 219 adult patients with CHB who underwent liver biopsy with simultaneous laboratory evaluation. Patients with viral co-infections (HIV, HCV, or HDV), metabolic syndrome, diabetes mellitus, hepatic steatosis, or incomplete data were excluded. Non-invasive fibrosis scores—including APRI, FIB-4, AST/ALT ratio (AAR), age–platelet index (API), GGT-to-platelet ratio (GPR), Lok index, modified Forns index, Albumin–Bilirubin (ALBI) score, and red cell distribution width (RDW)-based indices—were calculated using routine laboratory parameters. Histopathological fibrosis staging served as the reference standard. Diagnostic performance was evaluated using receiver operating characteristic (ROC) curve analysis, and areas under the curve (AUC) were compared using the DeLong test. Associations with histological activity index (HAI) were assessed using Spearman correlation. Results: For the prediction of significant fibrosis (≥F2), FIB-4 demonstrated the highest AUC, followed by ALBI and APRI. For advanced fibrosis (≥F3), FIB-4 again showed the highest AUC, followed by APRI and GPR. For significant fibrosis (≥F2), DeLong analysis revealed no statistically significant differences between FIB-4 and the other serum-based scores (p > 0.05). APRI (r = 0.556, p < 0.001) and FIB-4 (r = 0.463, p < 0.001) showed the strongest correlations with HAI. In ROC analysis for moderate-to-severe histological activity (HAI ≥ 4), APRI demonstrated the highest diagnostic accuracy (AUC = 0.677). Conclusions: Serum-based non-invasive fibrosis scores demonstrate comparable but overall modest diagnostic performance for biopsy-confirmed fibrosis in patients with chronic hepatitis B. Indices such as FIB-4 and APRI demonstrated relatively better discrimination and may be considered as screening or rule-out tools in selected clinical contexts. APRI and FIB-4 also show associations with histological activity; however, their clinical application should be interpreted with caution, given their moderate discriminatory capacity. Full article

Multi-agent and distributed dynamical systems can exhibit coordinated behavior that is difficult to summarize in terms of independent parts. Integrated Information Theory (IIT) provides one influential notion of system-level irreducibility, but exact computation of causal $\mathrm{\Phi }$ remains intractable except in very small systems. In this work, we introduce ${\mathrm{\Phi }}_{\mathrm{spectral}}$, a scalable observer-relative statistic defined on pairwise mutual information networks extracted from multivariate time-series data. A normalized graph Laplacian and its Fiedler vector identify a bipartition of the mutual information graph, and ${\mathrm{\Phi }}_{\mathrm{spectral}}$ reports the normalized weight of informational coupling crossing that cut. The measure is inspired by IIT’s concern with irreducibility but is not equivalent to intrinsic causal $\mathrm{\Phi }$: it is pairwise, undirected, and functional/statistical rather than intervention-based. We evaluate it on four exploratory simulation regimes: random oscillators, a transitional Kuramoto-like synchronization regime, a perfectly synchronized regime, and a combinatorial threshold-linear network (CTLN). Across these cases, ${\mathrm{\Phi }}_{\mathrm{spectral}}$ is most useful as a measure of observer-relative integration under second-order dependencies, separating redundancy-dominated from transiently differentiated regimes. The current results should be read as a proof-of-concept rather than as a formal validation against exact IIT. We discuss relations to weak IIT, Integrated World Modeling Theory (IWMT), and the perturbational complexity index (PCI), and we outline the stationary benchmarking and small-system validation needed for stronger causal claims. Full article

Sewerage systems are a main element of a city’s infrastructure. Roughness coefficients are fundamental parameters for sewage system operation. The intermittent nature of the flow leads to the appearance of deposits that become an integral part of the sewerage systems. Deposited material not only leads to the loss of hydraulic capacity and decreases the concentration of dissolved oxygen (which is found in direct relation to all quality parameters), but it also results in more transported particles being intercepted. In the design calculations, the roughness coefficient is estimated rather than calculated. It has been demonstrated that the estimation of stress within and above roughness elements improves the predictive capability for the concentration of suspended sediment. In this study, we focused on a local evaluation of the roughness coefficient when the flow velocity is below the minimum self-cleansing velocity. Some authors consider the selection of the most reliable method for estimating bed shear stress to be the main challenge. Other authors have suggested that all possible methods should be applied simultaneously to achieve a reliable bed shear stress estimation, knowing that the roughness coefficient can be determined through the shear boundary stress. We calculate the local roughness coefficient in Manning’s equation using a laboratory model, considering clear water flowing over a solid boundary with consolidated deposits, represented by artificial roughness elements (calibrated hemispheres). The European standard EN 752:2017 specifies a minimum average cross-sectional velocity of 0.7 m/s for pipe self-cleansing. This study established the range of possible roughness coefficient values when the minimum velocity design criterion is not met. The second criterion was to consider acceptable a sediment deposit occupying between 1% and 2% of the collector diameter. Velocity distributions around artificial roughness and statistical parameters of the turbulent flow were obtained using a PIV system. Five methods were implemented and the range of roughness coefficient values varied between 0.007 and 0.023. This variation is closely related to sewer performance. We selected the dissipation method as the primary reference for this study, as it is most closely aligned with the underlying physics of flow over roughness elements. This approach allows for robust validation by correlating multiple characteristic mechanisms of the turbulent cascade. Full article

Antimicrobial resistance represents a major global challenge for healthcare systems, particularly in urinary tract infections (UTIs), where empirical antibiotic therapy is frequently required. Acute pyelonephritis (AP) remains a severe condition, requiring prompt diagnosis and treatment. Local epidemiological data are essential for optimizing therapeutic strategies. The aim of this study was to analyze the pathogen distribution and antimicrobial resistance (AMR) patterns in patients with complicated AP. An observational, analytical study on community-acquired and hospital-acquired AP was conducted on patients admitted with complicated AP between January 2021 and December 2025. After applying the inclusions and exclusions criteria, 553 urinary isolates with complicated AP were analyzed to determine pathogen distribution and phenotypic AMR patterns derived from antimicrobial susceptibility testing. A total of 109 (19.7%) AMR isolates presented resistance phenotype. Resistant phenotypes were more frequently observed among male gender; age did not reach statistical significance. This study highlights the continued predominance of Escherichia coli in complicated AP while demonstrating a significant AMR burden among non-Escherichia coli pathogens, particularly Klebsiella and Pseudomonas species. These findings emphasize the importance of local epidemiological surveillance and culture-guided antibiotic therapy in the management of complicated UTIs. Full article

Animal poisoning remains an underreported public health and veterinary concern in many low- and middle-income countries where comprehensive surveillance systems are limited. This study was initiated to describe the epidemiology, clinical characteristics, and outcomes of animal poisoning cases reported to a national poison center in Thailand over a 10-year period. We performed a retrospective review of cases recorded in the Ramathibodi Poison Center Toxic Exposure Surveillance System between 2015 and 2024. Descriptive statistics were used to summarize species distribution, exposure categories, clinical signs, treatment, and outcomes, and comparisons were conducted to identify factors associated with mortality. A total of 118 poisoning cases were identified, with annual numbers increasing over time. Companion animals accounted for most exposures (93.2%), particularly dogs. Pesticides were the most common toxic agents, followed by household products, pharmaceuticals, and plant toxins. Neurological signs were the predominant clinical presentation. Respiratory compromise and neurological involvement at presentation were significantly associated with mortality. Overall survival was 88.1%. Fatalities were mainly linked to exposure to highly toxic pesticides or plants, including confirmed cassava-associated cyanide poisoning in elephants. This study highlights preventable environmental toxic risks affecting animals in Thailand and demonstrates the value of centralized poison surveillance. Strengthening pesticide safety practices and integrating veterinary toxicology into broader public health monitoring may reduce avoidable poisonings within shared human–animal environments. Full article

Balancing ecological conservation with agricultural production in protected areas remains a critical challenge, particularly regarding the nexus of food, water, land, and ecosystems (FWLE). Yet, the spatiotemporal trade-offs, synergies, and underlying drivers within the FWLE remain poorly understood. Focusing on the Henan Funiu Mountain National Nature Reserve (HFMNNR), we quantified water yield (WY), habitat quality (HQ), and food production (FP) using the InVEST model and statistical yearbook data. The XGBoost-SHAP framework was applied to dissect the key drivers and mechanisms governing the FWLE system. Results indicate a significant increasing trend in FP (2000–2020), contrasting with the unimodal (increase-then-decline) trajectories of HQ and WY. Pronounced trade-offs were identified between HQ and WY, and between HQ and FP. Topographic and vegetative factors predominated in shaping the spatial patterns of HQ and FP, whereas climatic factors dictated WY distribution. Specifically, HQ declined when NDVI fell below 0.87, population density surpassed 0.01, or slope was gentler than 7°. WY was constrained when precipitation dropped below 947 mm, actual evapotranspiration exceeded 752 mm, or temperature ranged between 12.5–16.2 °C. FP was suppressed under conditions of slopes > 7°, NDVI within 0–0.61 or 0.61–0.86, or DEM > 373 m. These findings underscore the necessity of spatially explicit management strategies grounded in spatial heterogeneity. We advocate for a multi-objective governance framework centered on HQ to harmonize production and ecological functions. Our findings provide critical insights for formulating policies aimed at sustainably managing protected areas facing similar ecological-production conflicts. Full article

Background and Methods: This prospective observational study investigated the prognostic value of the hemoglobin-to-red cell distribution width ratio (HRR) in 278 patients hospitalized with decompensated heart failure (HF). The primary endpoint was a composite of all-cause mortality or HF rehospitalization at 12 months. Multivariable Cox regression was employed to adjust for risk factors including age, sex, NT-proBNP, LVEF, and eGFR. Results: The median HRR was 0.89. During follow-up, the primary endpoint occurred in 167 (60.1%) patients. Unadjusted analysis showed a lower HRR was significantly associated with reduced event-free survival (log-rank p = 0.027). However, after multivariable adjustment, this association was no longer statistically significant (p = 0.240). Older age and male sex remained independent predictors. Conclusions: In patients with decompensated HF, a lower baseline HRR correlates with increased risk but does not maintain independent prognostic value after adjusting for powerful confounders. HRR may serve as a simple, initial marker of risk rather than an independent predictor. Full article

Dispersed archaeological landscapes are often rich in heritage value but weakly integrated into regional tourism systems. This creates difficulties in visitor orientation, interpretive continuity, and conservation-sensitive tourism planning. In response to this problem, this study examines the Adana–Kozan–Anavarza axis in southern Türkiye and proposes a spatial corridor framework for organising tourism development within a dispersed archaeological landscape. The research integrates spatial accessibility assessment, service-capacity evaluation, field observation, and sequential route design in order to establish a hierarchical gateway–transition–anchor configuration. Anavarza, one of the largest archaeological complexes of Cilicia, represents a monumental urban heritage site and a biocultural landscape situated within a Mediterranean ecological zone historically associated with Pedanius Dioscorides. Although current visitor volumes remain moderate, official statistics indicate a substantial increase in annual entries between 2022 and 2024, reflecting rising destination visibility. This emerging growth trajectory underscores the need for proactive spatial governance mechanisms prior to the onset of congestion and environmental degradation pressures. The findings suggest that Adana can function as a metropolitan gateway, Kozan as an intermediate staging node, and Anavarza as the archaeological anchor within a realistic multi-day visitor sequence. In this configuration, visitor functions are distributed across multiple nodes, while the ecological and archaeological sensitivity of the anchor landscape is more cautiously managed through spatial sequencing. Rather than proposing a predictive model, the study develops and assesses a context-responsive spatial planning framework grounded in accessibility, infrastructural feasibility, and conservation-sensitive visitor distribution. Beyond the local case, the study offers a transferable hierarchical staging logic for corridor-based heritage planning. Full article

Background: Adolescence is a critical developmental period during which dietary habits are formed and may influence long-term health outcomes. Monitoring changes in adolescents’ eating behaviors and nutrition-related knowledge over time is important for developing effective health promotion strategies. The aim of this study was to compare adolescents’ (Lower Silesia, Poland) dietary habits and nutritional knowledge between two study periods (2011 and 2023) using comparable survey methods. Methods: A repeated cross-sectional comparison of two independent cohorts was conducted using an identical questionnaire in both study periods. The 2023 cohort included 14-year-old primary school students (n = 100; 48 girls and 52 boys), while the comparison group consisted of adolescents aged 13–15 years assessed in 2011 (n = 377; 202 girls and 175 boys). Anthropometric measurements and self-reported data on dietary habits and nutritional knowledge were analyzed using descriptive statistics and group comparison tests. Results: The findings indicate changes in selected dietary behaviors and levels of nutritional knowledge among adolescents over the studied period. A higher percentage of students in 2023 reported eating four meals per day and obtaining information about healthy eating from the Internet rather than from television. Students in 2023 were also more likely to recognize the relationship between diet and attention, identify the harmful effects of energy drinks and excessive fast-food consumption, and provide correct answers regarding proper nutrition. Nutritional knowledge improved over time, with a mean percentage of correct responses of 71.9% in 2023 compared with 63.7% in 2011. Although nutritional awareness improved in several areas, certain unhealthy eating habits remained prevalent, including irregular breakfast consumption and frequent intake of sweets. Changes in the distribution of body weight categories were also observed, with gender-specific differences between cohorts. Conclusions: The results suggest that improvements in nutritional knowledge alone may not be sufficient to ensure positive changes in dietary behavior among adolescents. Continued monitoring of adolescent nutrition and the development of comprehensive health promotion strategies addressing both knowledge and environmental influences remain necessary. Full article

This work investigated the influence of thickness-direction boundary conditions on the flow characteristics of granular material in a quasi-two-dimensional silo using the discrete element method (DEM). Two types of boundary conditions were considered in the thickness direction: wall conditions and periodic boundary conditions. The simulation results indicate that under wall conditions, velocity waves propagate upward, manifested by the formation of bubble-like sub-flow zones in the velocity field, and the particle motion in the upper bed region exhibits a clear stick–slip feature. In contrast, under periodic boundary conditions, particle motion displays a resonant mode. Further statistical analysis reveals that, despite the distinct macroscopic motion mode under the two boundary conditions, the probability distributions of particle vertical fluctuating velocities share similar characteristics: both exhibit fat-tailed and asymmetric features and deviate from Gaussian distribution. Additionally, under wall conditions, the horizontal distributions of particle vertical velocity conform to the kinematic model throughout the bed, whereas under periodic boundary conditions, the horizontal distributions in the upper bed region display plug flow characteristics. In summary, the results of this work demonstrate that thickness-direction boundary conditions play a crucial role in determining the flow characteristics of granular assembly in silos. Full article

Heterogeneity at the grain scale strongly influences hydraulic fracturing in crystalline rock; however, systematic studies quantifying its impacts on the evolution of injection pressure and crack propagation remain limited. To address this gap, we employ a hydro-mechanical phase-field model incorporating Voronoi-based microstructures to systematically quantify the effects of grain-scale heterogeneity on hydraulic fracturing. Two numerical experimental programs are designed to examine the effects of (i) mean grain size and (ii) mineral distribution under different axial stresses. The simulations reveal a close coupling between injection pressure and crack-length evolution, and both responses are strongly governed by grain-scale heterogeneity. When the fracture enters weak minerals, it advances rapidly and pressure drops; when it encounters on strong minerals, growth slows or arrests and pressure builds until a threshold triggers the next advance. Moreover, peak pressure statistics further indicate that mineral distribution dominates the response scatter, while axial stress plays a secondary role. Specifically, the mean peak pressures at 0 and 10 MPa are similar (about 14.31 and 14.21 MPa), whereas rearranging minerals within the same Voronoi tessellation changes peak pressure by more than 4 MPa. Higher peaks occur when strong minerals lie ahead of the initial crack tip, increasing resistance to initiation and early growth. Finally, the stress state modulates fracture trajectories: under low axial stress, fractures preferentially follow mineral boundaries, whereas higher axial stress strengthens macroscopic stress guidance and shifts the path toward a direction closer to being perpendicular to the maximum principal stress. This trend is consistent with energy minimization, since interface detouring under high axial stress incurs a larger elastic free energy penalty. Full article

Background: This paper presents a systematic review and meta-analysis to identify the effects of Rhythmic Auditory Stimulation (RAS) delivered via wearable devices on the gait and balance in patients with Parkinson’s disease. Method: The PICO criteria were established according to the PRISMA 2020 guidelines, and literature searches were performed across five databases covering studies published between 2015 and 2025: PubMed, Embase, Cochrane, Scopus, and Web of Science. After applying the inclusion criteria, eleven randomized controlled trials (RCTs) were selected. The quality of the studies was evaluated using the PEDro Scale and ROB-2. Statistical analyses were performed using Review Manager 5.4 based on the number of samples, means, and standard deviations to calculate the effect sizes. Result: The analysis results showed that wearable RAS significantly improved the gait speed (SMD = 0.49, p < 0.05) and balance ability (SMD = 0.40, p < 0.05), while no significant differences in the gait pattern, FOG-Q, or UPDRS-III were observed. The heterogeneity among studies was low, and the funnel plots were distributed symmetrically, indicating minimal publication bias. The average PEDro score was 7.33, suggesting moderate-to-high methodological quality. Conclusion: wearable RAS was identified as an evidence-based intervention effective in improving the gait speed and balance in patients with Parkinson’s disease. Full article