Search Results (1,885)

Pomegranate marc is a major, underutilized juice industry by-product rich in lipophilic polyunsaturated fatty acids—notably conjugated α-linolenic acids (CLnAs)—and hydrophilic polyphenols with potent antioxidant and anti-inflammatory properties. Despite its potential for nutraceutical, cosmetic, and pharmaceutical applications, this matrix remains largely unexploited. This study presents a novel, sequential in-line extraction strategy combining supercritical CO₂ (ScCO₂) and subcritical water (scW) to recover complementary bioactive fractions. Both extraction steps were optimized via Response Surface Methodology (RSM). Box–Behnken optimization of ScCO₂ (43 MPa, 76 °C, 6.4 L min⁻¹, 124 min) yielded 30 g kg⁻¹ dry weight (dw) of oleoresin, achieving a 68% recovery of total oil. Subsequent scW extraction was optimized at 149 °C, with a 40 L kg⁻¹ water-to-solute ratio and 73 min extraction time, yielding 47 g kg⁻¹ dw of total phenolics (58% recovery). Strong agreement between experimental and predicted values confirmed the robustness of the models. Comprehensive profiling revealed a diverse phytocomplex including fatty acids, tocopherols, flavonoids, soluble sugars, and polysaccharides. Antioxidant assays confirmed that both γ-tocopherol and polyphenols significantly contribute to the extracts’ bioactivity. To improve physical handling, the aqueous fractions were converted into solid dispersions via spray drying with maltodextrin. Preliminary in vitro biological assessments on HEK-293 (human embryonic kidney) and MCF-7 (Michigan Cancer Foundation-7) cell lines suggested that the maltodextrin-based formulations may modulate the cytotoxic profile compared to the free extract, with exploratory results showing dosage-dependent variations in cell viability across the two lines. This work suggests a potentially scalable and sustainable biorefinery approach for the integral valorisation of pomegranate marc, offering a basis for a pathway to produce solvent-free bioactives. Full article

Red clover (Trifolium pratense L.) is a crop used as a forage that possesses an exceptional nutritional profile and digestibility. Unfortunately, this crop has low seed yield. Within the framework of the “Legume Generation” EC-funded project, our team aimed to investigate the role of foliar boron application on pollen viability and pollen tube development, and to assess its overall effect on red clover cultivation. Plants of six commercial diploid red clover cultivars, Nika 11, Sofia 52, AberClaret, Milvus, Global, and S123, were field-grown and boron-treated by spraying with the commercial product “Lebasol”, 11% active water-soluble boron. To reach our purpose, the transcript levels of genes related to flower, pollen, and pollen tube development and boron transport were measured by qRT-PCR; pollen grain viability and count were assessed microscopically. For this research, eight genes were selected: Auxin Response factor (TprARF17); TprAPETALA3; Walls are thin (TprWAT1 and TprWAT2); NIPs genes (Nodulin Intrinsic Protein) TprNIP4;2, TprNIP7;1, TprNIP5;1, and TprNIP6;1. Additionally, total nitrogen content in leaves detached from field-grown boron-treated and untreated plants was assessed and compared with the expression levels of two TprNIP5;1 and TprNIP6;1 transporters. The fresh and dry biomass weight from the first and second cuts was evaluated, as well as the seed collected from the red clover plants. Seed germination percentage and vigor of seedlings were examined in vitro for both boron-treated and untreated groups of two specific cultivars. Collected data confirm that foliar application of boron affects pollen viability and plant development of red clover in the cultivation conditions of South East Europe. Full article

Cardoon (Cynara cardunculus var. altilis DC.) is a Mediterranean crop valued for biomass production and bioactive compounds; however, information on the use of biostimulants in soilless systems remains limited. This study evaluated the effects of two biostimulants, a legume-derived protein hydrolysate (PH) and an Ascophyllum nodosum seaweed extract (SW), applied as weekly foliar sprays, on growth, physiological performance, mineral composition, and phytochemical traits of cardoon cultivated in a floating system. Biostimulant application significantly affected plant performance, inducing distinct treatment-dependent responses. Both PH and SW increased fresh and dry biomass compared with untreated plants. SW predominantly promoted vegetative growth, chlorophyll content, and nutrient accumulation, whereas PH markedly enhanced nutraceutical quality by increasing total phenolic content and antioxidant activity, reaching 64.4 mg GAE g⁻¹ dry extract and the lowest IC₅₀ value (172 µg mL⁻¹). Harvest timing modulated the magnitude of biostimulant effects, with maximum biomass yield observed at intermediate developmental stages (up to 8.17 kg m⁻²), while phenolic concentration and antioxidant capacity declined at later stages. Multivariate analyses confirmed that PH and SW induced complementary metabolic strategies. Overall, the biostimulant type emerged as the primary driver of plant response, with harvest timing acting as a modulating factor. Targeted biostimulant management, therefore, represents a promising strategy for optimizing the productivity and phytochemical quality of cardoon in soilless cultivation systems. Full article

Potassium-ion batteries hold great promise for large-scale energy storage, but their commercialization is hindered by the large ionic radius of potassium, which causes sluggish kinetics and severe volume expansion in anode materials. To address this, we present a scalable spray-drying strategy coupled with NaCl salt-templating to synthesize hierarchical porous carbon/vanadium sulfide microspheres (p-V₃S₄/C MS). In this structure, V₃S₄ nanoparticles are uniformly encapsulated within a dextrin-derived amorphous carbon matrix, and pores are formed via selective NaCl etching. This unique architecture accommodates volume fluctuations while providing rapid ion diffusion pathways. As a result, the p-V₃S₄/C MS anode exhibits outstanding electrochemical performance, maintaining a reversible capacity of 107 mA h g⁻¹ after 2000 cycles at 2.0 A g⁻¹, and achieves a high pseudocapacitive contribution of 93% at 2.0 mV s⁻¹. Furthermore, a full cell paired with a Prussian blue (PB) cathode demonstrates practical viability and robust reversibility. Our findings demonstrate that this structural engineering effectively mitigates internal resistance and structural degradation, offering a cost-effective route for mass-producing high-performance anodes for next-generation energy storage. Full article

This study developed an optimized processing strategy for γ-aminobutyric acid (GABA) instant white tea (GABA-IT) using GABA-enriched white tea as raw material, systematically characterizing its chemical composition and volatile profile. In contrast to the conventional instant tea production process, this work integrates response surface methodology with spray-drying parameter optimization. This integrated approach enables the simultaneous enhancement of functional components and sensory quality. A response surface design was employed to refine the extraction and spray-drying variables following preliminary single-factor trials, and the optimal parameter combination was subsequently determined (40% ethanol concentration, material-to-liquid ratio of 1:15, extraction time of 3 days, atomization speed of 300 rpm, and inlet temperature of 120 °C); the resulting GABA-IT exhibited significantly improved quality characteristics. Specifically, the GABA content increased by 209% (reaching 4.42 mg/g), and theanine, catechins, and caffeine were enriched by 200–300%. Regarding volatile profiles, processing led to a reduction in esters but an increase in aldehydes and hydrocarbons. Relative odor activity value (rOAV) analysis revealed that epoxy-β-ionone and linalool were the key contributors to the characteristic aroma of GABA-IT. Collectively, this study demonstrates the technical feasibility of producing GABA-rich instant tea with enhanced functional components and improved sensory quality, providing practical guidance for the large-scale industrial production of functional tea beverages. Full article

Mature-green tomatoes are prone to rapid ripening and quality deterioration during the postharvest stage, highlighting the urgent need for environmentally friendly and efficient preservation technologies. This study investigated the synergistic regulatory effects of nano-SiO₂ and light quality (white light, W; blue light, B; red/blue mixed light, RB, 1:1) on postharvest appearance, physiological processes, and quality attributes in ‘Yu Zhu’ (Solanum lycopersicum L.), a tasty tomato cultivar with light-yellow fruit color. Mature-green fruits were treated with light quality in combination with nano-SiO₂ (pre-immersion in 1 mL/L nano-SiO₂ for 1 h, followed by periodic spraying with 0.5 mL/L nano-SiO₂ every two days). Key indicators—including ripening traits, flavor attributes, antioxidant capacity, and endogenous hormone metabolites—were monitored on their respective sampling days. The results revealed distinct light quality-dependent responses: (1) B-Si (B + nano-SiO₂) significantly delayed the breaker stage compared to W, maintained the lowest water loss, and exhibited the slowest softening rate. W-Si showed a significantly higher dry weight-to-fresh weight ratio than W. (2) RB-Si achieved superior flavor quality, with 11.47% soluble solids, 1.62% titratable acidity, and a sugar-to-acid ratio of 7.2—values markedly higher than those in RB. (3) RB-Si increased total phenolic (TP), flavonoids, and ascorbic acid (AsA) levels relative to RB, while enhancing total antioxidant capacity (T-AOC) and the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), with only slight suppression of ascorbate peroxidase (APX) activity. (4) Nano-SiO₂ differentially regulated hormonal metabolism depending on light quality: it activated the jasmonic acid (JA)–gibberellin (GA) pathway under W light, fine-tuned cytokinin (CK) metabolism under B light, and upregulated JA, GA, CK, and auxin under RB light. Moreover, RB-Si significantly reduced ACC accumulation compared to W, thereby delaying senescence. Collectively, RB-Si synergistically regulates endogenous hormone metabolism to simultaneously delay ripening, reduce water loss, maintain firmness, optimize flavor, and enhance antioxidant capacity. This study elucidates the interaction mechanism between nano-SiO₂ and light quality, providing theoretical and technical support for the green preservation of horticultural crops. Full article

The incorporation of probiotic bacteria into active packaging systems represents an innovative strategy to enhance food preservation while delivering health benefits to consumers. This review discusses the selection criteria for probiotic strains focusing on their resistance to environmental stressors, antimicrobial activity, and viability in different food matrices and their integration into edible films and coatings. Polysaccharides, proteins, and hydrocolloids are widely used as biopolymeric matrices due to their biocompatibility and functional properties. The efficiency of probiotic packaging largely depends on three factors: the choice of strain, the encapsulation technique (such as spray drying, emulsification, or electrospinning), and the properties of the matrix material. These packaging systems demonstrate strong antimicrobial activity through multiple mechanisms, including bacteriocin production, competition for adhesion sites, and acidification. Applications in dairy, meat, fish, and fresh produce reveal the potential of these technologies to delay spoilage, reduce pathogenic microorganisms, inhibit lipid oxidation, and maintain nutritional and sensory qualities. Moreover, studies emphasize that combining probiotics with prebiotic compounds can improve both microbial stability and functional performance. Despite promising results, challenges remain regarding the industrial scalability and long-term stability of these systems under varied storage conditions. Future research should focus on optimizing formulation parameters, expanding applications across diverse food categories, and integrating smart packaging technologies. Altogether, probiotic-based edible packaging aligns with current demands for sustainable, health-oriented food solutions. Full article

The medical field now uses mRNA therapeutics to deliver fast programmable treatment options through versatile vaccination platforms. The worldwide adoption of mRNA therapeutics faces a major obstacle because these molecules require extreme cold storage and transportation systems. mRNA stability establishes a fundamental scientific and industrial challenge which requires researchers to unite formulation design with process control and material engineering for cold-chain independence. Current knowledge about RNA hydrolysis and lipid oxidation and water-mediated degradation is combined with new methods for solid-state stabilization through lyophilization and spray-freeze-drying and thin-film technologies. Mechanism such as vitrification, water replacement and excipient RNA interactions are assessed to establish the fundamental chemical properties needed for extended product stability. Advanced mRNA development strategies are also examined, including self-amplifying and circular RNA structures and nano-glass and metal–organic frameworks and artificial intelligence-based predictive design for creating stable mRNA formulations at room temperature. This review examines manufacturing and regulatory and logistical obstacles which affect real-world implementation of mRNA therapeutics through assessments of production scale and product quality tests and packaging strength and tropical environment testing. The combination of research findings presents a path to develop mRNA medicines which maintains their effectiveness when stored at 25 °C or above, thus enabling worldwide access to RNA-based treatments. The development of mRNA into a durable therapeutic platform requires scientists to merge molecular research with process development and regulatory standardization. Full article

Previous research indicates that WC-12Co contents above 60 wt.% in feedstock powders for cermet coatings impair adhesion and wear resistance. This study characterizes NiCrFeSiAlBC coatings—unreinforced or reinforced with 65 wt.% or 85 wt.% WC-12Co—applied via high-velocity oxy-fuel (HVOF) spraying onto stainless steel substrates under controlled parameters. It quantifies the influence of high carbide volume fractions within the NiCrFeSiAlBC matrix on microstructure and tribomechanical performance. Microstructural analysis revealed uniformly distributed cermet layers featuring dissolved reinforcements and WC hard phase formation, with minimal W₂C crystallization. Elevated WC-12Co incorporation promoted densification and reduced porosity. Vickers microhardness tests (HV 0.3) demonstrated increased hardness upon WC-12Co addition, attributable to finer particle sizes, lower porosity, and the presence of WC phases alongside crystallographic refinements. Under dry reciprocating sliding conditions, friction coefficients and wear volumes decreased markedly. Consequently, the coating with 85 wt.% WC exhibited the best mechanical and tribological properties. Full article

Excessive sodium consumption is a global public health problem that demands technological innovations in processed foods. This study aimed to reduce the sodium content in extruded corn snacks while maintaining perceived saltiness by substituting common salt with compound microparticles (70% NaCl, 30% starch). Two drying methods were evaluated: spray drying and conventional oven drying. The snacks were subjected to physicochemical, instrumental (texture and colour), density, porosity, microstructural, and sensory analyses (intensity scale, n = 104). The results demonstrated that the particles obtained by spray drying allowed a 28% reduction in the final sodium content without statistically differing in saltiness perception compared to the control. In contrast, the oven treatment reduced saltiness perception compared to the standard. Images obtained by scanning electron microscopy, along with porosity measurements, demonstrated a significant increase in porosity in the spray-dried sample. This allows rapid dissolution of the salt in the mouth, maintaining a salty taste even with reduced sodium content. It was concluded that the use of salt–starch microparticles via spray drying was a viable strategy for producing snacks with reduced sodium content without compromising sensory quality. Full article

Background/Objectives: Reproducible evaluation of aerosol dispersibility remains a key challenge in the development of dry powder inhalers (DPIs), where small variations in particle cohesion, morphology, or device resistance can lead to large differences in aerodynamic performance. In passive DPIs, the forces required for powder fluidization and aerosolization arise from the interaction of patient inspiratory airflow with device geometry and must overcome strong interparticle cohesive forces to enable effective lung delivery. Cascade impaction is the gold standard for determining aerodynamic particle size distribution (APSD), but its low throughput and experimental burden limit its utility for systematic formulation and device screening. Prior studies have explored laser diffraction-based particle sizing under varying dispersion energies as indirect metrics of powder dispersibility. Here, we extend this approach by introducing a mathematically rigorous, distribution-based framework that applies the first-order Wasserstein distance (Earth Mover’s Distance) to quantify relative dispersibility with respect to a material-specific maximally dispersed reference state. Methods: Mannitol, trehalose, and inulin were spray-dried under matched conditions to generate model dry powders. Particle size distributions were measured by laser diffraction (Sympatec HELOS/R) using both a RODOS dry dispersion module to define a maximally dispersed reference state and an INHALER module to generate aerosols under clinically relevant dispersion conditions spanning multiple device resistances and pressure drops. For each condition, the Wasserstein-1 distance (W₁) was computed between cumulative volume-based size distributions obtained under reference and inhaler-based dispersion. Cascade impaction was used as an orthogonal method to characterize aerodynamic performance under a representative dispersion condition. Results: W₁ captured formulation-, device-, and flow-dependent differences in dispersibility that were not readily separable by visual inspection of particle size distributions alone. Crystalline mannitol exhibited the largest and most flow-rate-dependent W₁ values, whereas amorphous trehalose and polymeric inulin showed smaller W₁ values with distinct, non-monotonic pressure responses that depended on device resistance. W₁ qualitatively aligned with cascade impaction metrics, exhibiting a positive association with mass median aerodynamic diameter and an inverse association with fine particle fraction, while also demonstrating that efficient dose emission can occur despite incomplete deagglomeration. Conclusions: This study establishes the Wasserstein distance as a physically interpretable, formulation-agnostic metric for quantifying aerosol dispersibility relative to a material-specific reference state. This framework enables systematic comparison of dispersion efficiency across devices and operating conditions using standard laser diffraction data and provides a reproducible basis for mechanistic optimization of DPI formulations and inhaler designs. Full article

Steel structures in marine splash zones (MSZ) experience severe corrosion owing to high humidity and frequent wet–dry cycles, which poses considerable threats to structural integrity and operational safety. To achieve intelligent, real-time corrosion monitoring, this study presents a corrosion-rate model based on the Weibull distribution, intended to serve as the core algorithm of smart corrosion sensors that continuously provide corrosion depth data via techniques such as electrochemical impedance spectroscopy or fiber optic sensing. The model was validated through systematic laboratory salt-spray cyclic tests that simulated MSZ conditions; corrosion behaviour was analysed by means of mass-loss measurements, electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). The results reveal a three-stage corrosion progression and confirm that the Weibull model accurately captures the time-variant corrosion behaviour under different splash intensities. The model thus provides a reliable algorithmic foundation for intelligent corrosion monitoring, enabling real-time assessment of structural safety and prediction of residual life. Full article

The present study explores the possibility of combining membrane concentration, spray drying, and low-temperature precipitation into a single process for the valorization of spent lavender biomass as a source of ingredients rich in antioxidants. Lavender spent plant material was subjected to solid–liquid extraction, and the obtained hydroalcoholic extracts were further concentrated using a dead-end membrane filtration cell (METcell) with a polyamide–urea thin-film composite X201 membrane. The feed and the obtained retentate were subsequently spray dried using a Nano Spray Dryer B-90 (BÜCHI) under different temperature conditions (120 °C and 85 °C). Low-temperature precipitation was further applied for the retentate. An eight-fold concentration of the extracts was achieved, with membrane rejection coefficients of 100% for antioxidant activity and 98.5% for dry solids content. The permeate flux ranged from 2.25 to 0.201 L·m⁻²·h⁻¹. Spray drying at a lower inlet temperature resulted in minimal losses for antioxidant activity (below 6%). The low-temperature storage of the membrane concentrate led to clear phase separation, allowing for the recovery of a precipitated fraction. The obtained results demonstrate that the integrated approach may support the sustainable and scalable valorization of lavender by-products. Full article

Cinnamaldehyde (CIN) is a natural organic compound known for its antimicrobial and antioxidant properties. However, its susceptibility to environmental degradation has restricted its practical application. This study aimed to microencapsulate CIN using soy protein isolate hydrolysates and maltodextrin as wall materials through emulsion preparation and spray drying, and to characterize the microstructure, controlled-release properties, antibacterial efficacy, and preservation performance of the resulting microcapsules. Under optimized condition, the encapsulation efficiency reached 70.72%. The microcapsules displayed smooth spherical structures, improved thermal stability, and an average particle size of 291.01 ± 33.64 nm. They demonstrated enhanced storage stability and sustained-release characteristics. Furthermore, the microcapsules exhibited significant antibacterial and antioxidant activity, which effectively delayed lipid and protein oxidation in pork loin for up to 6 days. Collectively, the results confirm the successful encapsulation of CIN and indicate the strong potential of these microcapsules for food industry applications requiring preservative and controlled-release functions. Full article

Pulmonary drug delivery has emerged as a powerful strategy for the treatment of respiratory infectious diseases, including bacterial, fungal, and viral infections such as influenza and COVID-19, by enabling high local drug concentrations while minimizing systemic exposure. However, the clinical success of inhaled anti-infective therapies critically depends on the precise engineering of particle properties that govern lung deposition, cellular targeting, and therapeutic efficacy. In this review, we provide a comprehensive and technology-driven overview of cutting-edge formulation and manufacturing strategies for pulmonary drug delivery, with particular emphasis on the key process and formulation parameters required to generate effective inhalable systems for the treatment of infectious diseases. Advanced particle-engineering approaches, including spray drying, spray freeze drying, jet milling, and supercritical fluid technologies are discussed as enabling tools to tightly control aerodynamic particle size, morphology, and solid-state properties. In parallel, emerging platforms such as nanoparticle-based delivery systems are examined for their ability to target specific lung cell populations, including epithelial cells and alveolar macrophages, thereby enhancing antimicrobial efficacy. Finally, innovative manufacturing concepts such as microfluidics and three-dimensional (3D) printing are highlighted as promising strategies to improve particle size uniformity, reproducibility, and formulation customization. By integrating formulation science with advanced manufacturing technologies, this review identifies the critical design and processing parameters that underpin effective pulmonary delivery of anti-infective therapies and outlines future directions for the development of next-generation inhaled treatments. Full article