Search Results (672)

The chickpea (Cicer arietinum L.) is a key legume crop in Mediterranean agriculture, valued for its nutritional profile and adaptability. However, its productivity is severely impacted by drought stress. To identify microbial solutions that enhance drought resilience, we isolated seven Mesorhizobium strains from chickpea nodules collected in southern Spain and evaluated their cultivar-specific symbiotic performance. Two commercial cultivars (Pedrosillano and Blanco Lechoso) and twenty chickpea germplasms were tested under growth chamber and greenhouse conditions, both with and without drought stress. Initial screening in a sterile substrate using nodulation assays, shoot/root dry weight measurements, and acetylene reduction assays identified three elite strains (ISC11, ISC15, and ISC25) with superior symbiotic performance and nitrogenase activity. Greenhouse trials under reduced irrigation demonstrated that several strain–cultivar combinations significantly mitigated drought effects on plant biomass, with specific interactions (e.g., ISC25 with RR-98 or BT6-19) preserving over 70% of shoot biomass relative to controls. Whole-genome sequencing of the elite strains revealed diverse taxonomic affiliations—ISC11 as Mesorhizobium ciceri, ISC15 as Mesorhizobium mediterraneum, and ISC25 likely representing a novel species. Genome mining identified plant growth-promoting traits including ACC deaminase genes (in ISC11 and ISC25) and genes coding for auxin biosynthesis-related enzymes. Our findings highlight the potential of targeted rhizobial inoculants tailored to chickpea cultivars to improve crop performance under water-limiting conditions. Full article

During the Pleistocene, the successive ice ages prompted the southward expansion of the “Mammoth Steppe” ecosystem, a prevalent habitat that supported species adapted to cold environments such as the mammoth, woolly rhinoceros, and reindeer. Previously, the earliest evidence for such cold-adapted species in the Iberian Peninsula dated back to Marine Isotope Stage 6 (MIS 6, ~191–123 ka). This paper reports the discovery of a reindeer (Rangifer) tooth from Unit GIII of the Galería site at the Atapuerca-Trinchera site complex, dated to MIS 8 (~300–243 ka). This find is significant as it represents not only the oldest evidence of glacial fauna in the Iberian Peninsula but also the southernmost occurrence of reindeer in Europe of this age. The presence of Rangifer at this latitude (42°21′ N) during MIS 8 suggests that the glacial conditions affected the Iberian fauna earlier and with greater intensity than previously understood. Over the subsequent climatic cycles, cold-adapted species spread further south, reaching Madrid (40°20′) during the penultimate glacial period and the province of Granada (37°01′) during the last glacial maximum. The coexistence of human fossils and lithic artefacts within Units GII and GIII at Galería indicates that early humans also inhabited these glacial environments at Atapuerca. This study elaborates on the morphological and archaeological significance of the reindeer fossil, emphasizing its role in understanding the biogeographical patterns of glacial fauna and the adaptability of Middle Pleistocene human populations. Full article

Extreme heat is a pressing public health threat. This study assesses and describes the interrelationships between beliefs about heat waves, individuals’ precautionary behaviors during heat waves, and demographic factors. In May 2022, we surveyed 1051 residents (aged 25–90 years) in Southern Spain, a region that experiences frequent heat waves. We found that many participants engaged in heat wave avoidance (80.5%, e.g., spending more time indoors), impact reduction (63.7%, e.g., drinking more water), or prosocial behavior (31.6%, e.g., helping others). However, one in four (25.9%) respondents also indicated that they personally do not need to worry about heat waves. Heat wave beliefs and behaviors were modestly correlated with demographic characteristics. Individuals who view themselves as less vulnerable to heat-related health risks (“impervious” beliefs) were less likely to report altering their behavior during heat waves. Public health efforts aiming to change behavior during heat waves may anticipate “impervious” beliefs and demographic differences in risk perception and heat-related behaviors. Full article

Q Fever is a zoonosis caused by Coxiella burnetii that affects domestic and wild ruminants, leading to reproductive disorders. In humans, the disease can manifest with acute and chronic clinical manifestations. Veterinarians, as healthcare professionals in close contact with animals, serve both as the first line of defence in preventing infection at the animal–human interface and as an important sentinel group for the rapid detection of outbreaks. The aim of this study was to assess the knowledge, perception, and attitude of veterinarians in Southern Spain regarding Q Fever. To this end, an online survey was designed, validated, and conducted among veterinarians in the province of Malaga, with a final participation of 97 individuals, predominantly from the private sector (clinic, livestock, agri-food, etc.). The data obtained reflected a general lack of knowledge about the disease, particularly concerning its epidemiology and infection prevention. Regarding perception and attitude, a significant percentage of respondents stated they did not use protective equipment when handling susceptible animals and only sought information about the disease in response to outbreak declarations. The study emphasised the significance of promoting training in zoonotic diseases during and after graduation, the relevance of official channels in occupational risk prevention, and the utility of epidemiological surveys as a tool to identify and address potential gaps in knowledge related to this disease. Full article

Glyphosate has been used for roadside weed control in southern Spain for over 40 years, and most populations of goldentop (Lamarckia aurea L.) Moench have putatively developed resistance to this active ingredient. The physiological and biochemical basis for glyphosate resistance in this weed has been investigated. Dose–response studies indicated that the resistant biotype (R) was almost 13 times more resistant to glyphosate compared to a known susceptible biotype (S). Studies of foliar glyphosate retention and ¹⁴C-glyphosate uptake/translocation showed no significant differences between both L. aurea biotypes. Basal 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) activity (µmol µg⁻¹TSP min⁻¹) showed similar values between R (0.82 ± 0.04) and S (0.75 ± 0.05) biotypes. On the other hand, the resistance factor (I₅₀R/I₅₀S) did not show a difference between the two biotypes. Therefore, it was concluded that target-site (TSR) resistance mechanisms are not involved in glyphosate resistance in this weed species. The metabolism of glyphosate to form the non-toxic metabolites aminomethylphosphonic acid (AMPA), glyoxylate, and sarcosine was greater and faster in the R compared to the S biotype; thus, glyphosate resistance is due to non-target-site resistance (NTSR) mechanisms. This paper is the first report of glyphosate resistance in L. aurea in the world. Full article

Climate change poses a major threat to forests, impacting the distribution and viability of key species. Quercus pyrenaica Willd., a marcescent oak endemic to the Iberian Peninsula (Portugal and Spain) and southwestern France and a structural species in submediterranean forests, is particularly susceptible to shifts in temperature and precipitation patterns. Aiming to assess its potential loss of suitable area under future climate scenarios, we developed high-resolution spatial distribution models to project the future habitat suitability of Q. pyrenaica under two climate change scenarios (SSP3-7.0 and SSP5-8.5) for the periods 2070 and 2100. Our model, which has an excellent predictive performance (AUC of 0.971 and a TSS of 0.834), indicates a predominantly northward shift in the potential distribution of the species, accompanied by substantial habitat loss in southern and lowland regions. Long-term potential suitable area may shrink to 42% of that currently available. This, combined with the limited natural dispersal capacity of the species, highlights the urgency of targeted management and conservation strategies. These results offer critical insights to inform conservation strategies and forest management under ongoing climate change. Full article

In this study, we examined the microclimates at eight entrances to a karst system distributed between an elevation of 812 and 906 m in Southern Spain. The karst system, characterised by subvertical open tectonic joints that form narrow shafts, developed on the slope of a mountainous area with a Mediterranean climate and strong chimney effect, resulting in an intense airflow throughout the year. The airflows modify the entrance temperatures, creating a distinctive pattern in each opening that changes with the seasons. The objective of this work is to characterise the outflows and find simple temperature-based parameters that provide information about the karst interior. The entrances were monitored for five years (2017–2022) with temperature–humidity dataloggers at different depths. Other data collected include discrete wind measurements and outside weather data. The most significant parameters identified were the characteristic temperature (T_y), recorded at the end of the outflow season, and the rate of cooling/warming, which ranges between 0.1 and 0.9 °C/month. These parameters allowed the entrances to be grouped based on the efficiency of heat exchange between the outside air and the cave walls, which depends on the rock-boundary geometry. This research demonstrates that simple temperature studies with data recorded at selected positions will allow us to understand geometric aspects of inaccessible karst systems. Dynamic high-airflow cave systems could become a natural source of evidence for climate change and its effects on the underground world. Full article

As climate change intensified water scarcity in Southern Europe, tourism-dependent regions such as Portugal’s Algarve faced growing pressure to adapt their water management systems. This study investigated how hotel groups in the Algarve have adopted and communicated water reuse technologies—specifically desalination and greywater recycling—under environmental, institutional, and reputational constraints. A comparative qualitative case study was conducted involving three hotel groups—Vila Vita Parc, Pestana Group, and Vila Galé—selected through purposive sampling based on organizational capacity and technology adoption stage. The analysis was supported by a supplementary mini-case from Mallorca, Spain. Publicly accessible documents, including sustainability reports, media coverage, and policy frameworks, were thematically coded using organizational environmental behavior theory and the OECD Principles on Water Governance. The results demonstrated that (1) higher organizational capacity was associated with greater maturity in water reuse implementation; (2) communication transparency increased alongside technological advancement; and (3) early-stage adopters encountered stronger financial, regulatory, and operational barriers. These findings culminated in the development of the Maturity–Communication–Governance (MCG) Framework, which elucidates how internal resources, stakeholder signaling, and institutional alignment influence sustainable infrastructure uptake. This research offered policy recommendations to scale water reuse in tourism through financial incentives, regulatory simplification, and public–private partnerships. The study contributed to the literature on sustainable tourism and decentralized climate adaptation, aligning with UN Sustainable Development Goals 6.4, 12.6, and 13. Full article

The Adiabatic Sonic Evaporation and Crystallization (ASEC) technology was developed as a disruptive zero-liquid discharge system to treat contaminated mining effluents. This study evaluates its ecotoxicological efficacy using Lemna minor, a freshwater macrophyte, as a sensitive bioindicator. Acute growth inhibition tests were conducted using OECD Guideline 221. Lemna minor was exposed for 7 days to untreated and treated effluents from the Tharsis mine and the Tinto River in southern Spain. The results revealed 100% inhibition of frond growth and biomass in untreated samples (pH < 2.6), indicating acute toxicity. In contrast, effluents treated with ASEC showed growth and biomass accumulation statistically indistinguishable from the control, confirming the system’s efficiency in reducing toxicity and restoring water quality. These findings support the environmental viability of ASEC technology for mine and port effluent treatment. Full article

This article investigates the institutional and informational foundations of water governance in Southern Europe amid escalating climate stress. Focusing on Portugal, Spain, Italy, and Greece, it develops a multi-level analytical framework to explore how information asymmetries and governance fragmentation undermine coordinated responses to water scarcity. Integrating theories of information economics, polycentric governance, and critical institutionalism, this study applies a stylized economic model and comparative institutional analysis to assess how agents—such as farmers, utilities, regulators, and civil society—respond to varying incentives, data access, and coordination structures. Using secondary data, normalized indicators, and scenario-based simulations, the model identifies three key structural parameters—institutional friction (θ_i), information cost (β_i), and incentive strength (α_i)—as levers for governance reform. The simulations are stylized and not empirically calibrated, serving as heuristic tools rather than predictive forecasts. The results show that isolated interventions yield limited improvements, while combined reforms significantly enhance both equity and effectiveness. Climate stress simulations further reveal stark differences in institutional resilience, with Greece and Italy showing systemic fragility and Portugal emerging as comparatively robust. This study contributes a flexible, policy-relevant tool for diagnosing governance capacity and informing reform strategies while also underscoring the need for integrated, equity-oriented approaches to adaptive water governance. Full article

This study presents a social life cycle assessment (S-LCA) of the F-CUBED Production System (FPS), an innovative process that converts wet biogenic residues—specifically paper biosludge, virgin olive pomace, and fruit and vegetable residues—into intermediate bioenergy carriers via hydrothermal treatment (TORWASH^®), pelletization, and anaerobic digestion. The hydrothermal carbonization of these low-grade, moisture-rich biogenic residues enhances the flexibility and reliability of renewable energy systems while also offering the potential to reduce environmental burdens compared to conventional disposal methods. Through this S-LCA, the study aims to evaluate the cradle-to-gate socioeconomic impacts of the FPS in three European contexts—Sweden, Italy, and Spain—using the 2020 UNEP Guidelines and the Social Hotspots Database (SHDB) and applying quantitative modeling via SimaPro. The functional unit is defined as 1 kWh of electricity produced. The assessment combines SHDB-based modeling with primary data from stakeholder surveys conducted in the three countries. Impact categories are harmonized between SHDB and UNEP typologies, and the results are reported in medium-risk-hour equivalents (mrheq). The results show a heterogeneous social impact profile across case studies. In Sweden, the treatment of paper biosludge delivers substantial benefits with minimal risk. In Spain (orange peel), the introduction of the FPS demonstrated a strong social benefit, particularly in health and safety and labor rights, indicating high institutional performance and good integration with local industry. Conversely, in Italy (olive pomace), the FPS revealed significant social risks, especially in the biopellet production and electricity generation sectors, reflecting regional vulnerabilities in labor conditions and governance. This suggests that targeted mitigation strategies are recommended in contexts like Southern Italy. These findings highlight that the social sustainability of emerging bioenergy technologies is context-dependent and sensitive to sectoral and regional socioeconomic conditions. This S-LCA complements prior environmental assessments and emphasizes the importance of integrating social performance considerations in the deployment and scaling of innovative bioenergy systems. Full article

Equine piroplasmosis (EP) is an endemic parasitic disease in southern European countries, such as Spain. Andalusia, the most southwestern region of Europe, is the community with the highest number of registered horses and farms in Spain and one of the main international exporters of Andalusian (Spanish Purebred) horses worldwide. Considering the current expansion of this disease and the possible effect of climate change on its prevalence, studying the EP prevalence in this region is compelling. Molecular (PCR) and serological methods (cELISA and IFAT) were used to study the true and apparent prevalences during a period of three consecutive years, evaluating the effects of age, sex, season, year of testing, and province. Results showed different EP prevalences between western and eastern provinces, as well as among seasons. Moreover, a positive association was observed between age and T. equi seropositivity, without any effect of sex. Our findings demonstrate that Andalusia is an EP endemic region, but prevalences were lower compared to central and northern Spanish regions. Moreover, EP prevalence has not increased in Andalusia in recent years despite climate changes. Full article

This study explores the water harvesting systems of mgouds in southern Tunisia and boqueras in southeastern Spain to understand their adaptation to semi-arid conditions and geomorphic contexts. These systems use ephemeral water through medieval-origin infrastructures to increase the water supply to rainfed crops. The hypothesis is that the diversity of these systems stems from environmental rather than cultural factors. By employing a qualitative–analytical approach, this study compares concentrated runoff diversion systems to investigate the use of boqueras/mgouds in terraces and glacis in the arid and semi-arid areas of Tunisia and the southeastern Iberian Peninsula. The research involved performing detailed geomorphological and climatological analyses and comparing structural complexities and water management strategies across different regions. The results indicate significant variability in system size and complexity. Tunisian mgouds are typically simpler and more individualised, while Spanish boqueras are larger and more complex due to more frequent and intense torrential rainfall. No common patterns were identified between the two regions. This study reveals that both types of systems reflect sophisticated adaptations to manage water scarcity and mitigate the impacts of intense rainfall, with geomorphic and climatic factors playing a decisive role. The primary conclusion is that the design and functionality of these water systems are predominantly influenced by environmental conditions rather than cultural factors. This research provides insights for developing sustainable water management strategies in other semi-arid regions. Full article

Background: Understanding the multifactorial determinants of human longevity remains a major scientific challenge. Certain regions of the world—so-called “longevity hotspots”—exhibit a notably high prevalence of centenarians; one such region is the province of Ourense, in north-western Spain. Objectives: This study aimed to analyse, for the first time, the nutritional factors associated with healthy longevity among centenarians, as well as those linked to longevity irrespective of health status, in the province of Ourense. Methods: A cross-sectional, retrospective, observational, mixed-methods study was conducted. A population of 261 individuals aged 100 or over residing in Ourense was identified. A sample of 156 participants was included in the quantitative analysis; from this sample, 25 centenarians were selected for in-depth qualitative analysis through personal interviews. Results: Dietary patterns aligned with the Southern European Atlantic Diet (SEAD), combined with strong social bonds and a culture of self-sufficiency, appear to be key contributors to exceptional longevity in this population. Conclusions: Remarkable longevity in Ourense is associated with a combination of factors: adherence to an SEAD-style dietary pattern, an active and uncomplicated lifestyle, and strong social support networks. Full article

This study investigated the mechanisms determining macrophyte species composition in 23 Andalusian Mediterranean mountain wetlands (southern Spain). We employed a methodology combining two approaches: a pattern-based approach utilizing Elements of Metacommunity Structure (EMS) and a mechanistic approach involving Redundancy Analysis (RDA) and variance partitioning. This allowed us to identify the relevance of interactions between environmental and spatial factors. Data collection in these wetlands included macrophyte samples and physicochemical variables, alongside spatial variables generated using Moran’s Eigenvector Maps (MEMs). To refine the analysis of metacommunity structuring, the species matrix was partitioned based on macrophyte dispersal strategy (charophytes by spores and macrophyte vascular plants by seeds). Our results reveal that the macrophyte metacommunity in these wetlands exhibits quasi-clumped species loss for the total community, while charophytes and vascular plants showed quasi-random species loss. In conclusion, this study demonstrates that macrophyte communities in Mediterranean mountain wetlands do not follow a simple species replacement pattern. Instead, they are organized in a quasi-nested pattern, strongly shaped by environmental filters and, to a lesser extent, by spatial connectivity, with a prominent role for random processes. Understanding these mechanisms is crucial for predicting species responses to environmental changes and for designing effective conservation strategies within these vulnerable ecosystems. Full article