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Keywords = soil improvement

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23 pages, 12563 KiB  
Article
Optimization of Grouser–Track Structural Parameters for Enhanced Tractive Performance in Unmanned Amphibious Tracked Vehicles
by Yaoyao Chen, Xiaojun Xu, Wenhao Wang, Xue Gao and Congnan Yang
Actuators 2025, 14(8), 390; https://doi.org/10.3390/act14080390 - 6 Aug 2025
Abstract
This study focuses on optimizing track and grouser structural parameters to enhance UATV drawbar pull, particularly under soft soil conditions. A numerical soil thrust model for single-track shoes was developed based on track–soil interaction mechanics, revealing distinct mechanistic roles: track structural parameters (length/width) [...] Read more.
This study focuses on optimizing track and grouser structural parameters to enhance UATV drawbar pull, particularly under soft soil conditions. A numerical soil thrust model for single-track shoes was developed based on track–soil interaction mechanics, revealing distinct mechanistic roles: track structural parameters (length/width) govern pressure–sinkage relationships at the track base, while grouser structural parameters (height, spacing, V-shaped angle) dominate shear stress–displacement dynamics on grouser shear planes. A novel DEM-MBD coupling simulation framework was established through soil parameter calibration and multi-body dynamics modeling, demonstrating that soil thrust increases with grouser height and V-shaped angle, but decreases with spacing, with grouser height exhibiting the highest sensitivity. A soil bin test validated the numerical model’s accuracy and the coupling method’s efficacy. Parametric optimization via the Whale Optimization Algorithm (WOA) achieved a 55.86% increase in drawbar pull, 40.38% reduction in ground contact pressure and 57.33% improvement in maximum gradability. These advancements substantially improve the tractive performance of UATVs in soft beach terrains. The proposed methodology provides a systematic framework for amphibious vehicle design, integrating numerical modeling, high-fidelity simulation, and experimental validation. Full article
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20 pages, 1788 KiB  
Article
Legume–Cereal Cover Crops Improve Soil Properties but Fall Short on Weed Suppression in Chickpea Systems
by Zelalem Mersha, Michael A. Ibarra-Bautista, Girma Birru, Julia Bucciarelli, Leonard Githinji, Andualem S. Shiferaw, Shuxin Ren and Laban Rutto
Agronomy 2025, 15(8), 1893; https://doi.org/10.3390/agronomy15081893 - 6 Aug 2025
Abstract
Chickpea is a highly weed-prone crop with limited herbicide options and high labor demands, raising the following question: Can fall-planted legume–cereal cover crops (CCs) improve soil properties while reducing herbicide use and manual weeding pressure? To explore this, we evaluated the effect of [...] Read more.
Chickpea is a highly weed-prone crop with limited herbicide options and high labor demands, raising the following question: Can fall-planted legume–cereal cover crops (CCs) improve soil properties while reducing herbicide use and manual weeding pressure? To explore this, we evaluated the effect of fall-planted winter rye (WR) alone in 2021 and mixed with hairy vetch (HV) in 2022 and 2023 at Randolph farm in Petersburg, Virginia. The objectives were two-fold: (a) to examine the effect of CCs on soil properties using monthly growth dynamics and biomass harvested from fifteen 0.25 m2-quadrants and (b) to evaluate the efficiency of five termination methods: (1) green manure (GM); (2) GM plus pre-emergence herbicide (GMH); (3) burn (BOH); (4) crimp mulch (CRM); and (5) mow-mulch (MW) in suppressing weeds in chickpea fields. Weed distribution, particularly nutsedge, was patchy and dominant on the eastern side. Growth dynamics followed an exponential growth rate in fall 2022 (R2 ≥ 0.994, p < 0.0002) and a three-parameter sigmoidal curve in 2023 (R2 ≥ 0.972, p < 0.0047). Biomass averaged 55.8 and 96.9 t/ha for 2022 and 2023, respectively. GMH consistently outperformed GM in weed suppression, though GM was not significantly different from no-till systems by the season’s end. Kabuli-type chickpeas under GMH had significantly higher yields than desi types. Pooled data fitted well to a three-parametric logistic curve, predicting half-time to 50% weed coverage at 35 (MM), 38 (CRM), 40 (BOH), 46 (GM), and 53 (GMH) days. Relapses of CCs were consistent in no-till systems, especially BOH and MW. Although soil properties improved, CCs alone did not significantly suppress weed. Full article
(This article belongs to the Section Weed Science and Weed Management)
22 pages, 19937 KiB  
Article
Development and Evaluation of a Two-Dimensional Extension/Contraction-Driven Rover for Sideslip Suppression During Slope Traversal
by Kenta Sagara, Daisuke Fujiwara and Kojiro Iizuka
Aerospace 2025, 12(8), 699; https://doi.org/10.3390/aerospace12080699 - 6 Aug 2025
Abstract
Wheeled rovers are widely used in lunar and planetary exploration missions owing to their mechanical simplicity and energy efficiency. However, they face serious mobility challenges on sloped soft terrain, especially in terms of sideslip and loss of attitude angle when traversing across slopes. [...] Read more.
Wheeled rovers are widely used in lunar and planetary exploration missions owing to their mechanical simplicity and energy efficiency. However, they face serious mobility challenges on sloped soft terrain, especially in terms of sideslip and loss of attitude angle when traversing across slopes. Previous research proposed using wheelbase extension/contraction and intentionally sinking wheels into the ground, thereby increasing shear resistance and reducing sideslip. Building upon this concept, this study proposes a novel recovery method that integrates beam extension/contraction and Archimedean screw-shaped wheels to enable lateral movement without rotating the rover body. The beam mechanism allows for independent wheel movement, maintaining stability by anchoring stationary wheels during recovery. Meanwhile, the helical structure of the screw wheels helps reduce lateral earth pressure by scraping soil away from the sides, improving lateral drivability. Driving experiments on a sloped sandbox test bed confirmed that the proposed 2DPPL (two-dimensional push-pull locomotion) method significantly reduces sideslip and prevents a drop in attitude angle during slope traversal. Full article
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20 pages, 5378 KiB  
Article
Machine Learning-Based Approach for CPTu Data Processing and Stratigraphic Analysis
by Helena Paula Nierwinski, Arthur Miguel Pereira Gabardo, Ricardo José Pfitscher, Rafael Piton, Ezequias Oliveira and Marieli Biondo
Metrology 2025, 5(3), 48; https://doi.org/10.3390/metrology5030048 - 6 Aug 2025
Abstract
Cone Penetration Tests with pore pressure measurements (CPTu) are widely used in geotechnical site investigations due to their high-resolution profiling capabilities. However, traditional interpretation methods—such as the Soil Behavior Type Index (Ic)—often fail to capture the internal heterogeneity typical of [...] Read more.
Cone Penetration Tests with pore pressure measurements (CPTu) are widely used in geotechnical site investigations due to their high-resolution profiling capabilities. However, traditional interpretation methods—such as the Soil Behavior Type Index (Ic)—often fail to capture the internal heterogeneity typical of mining tailings deposits. This study presents a machine learning-based approach to enhance stratigraphic interpretation from CPTu data. Four unsupervised clustering algorithms—k-means, DBSCAN, MeanShift, and Affinity Propagation—were evaluated using a dataset of 12 CPTu soundings collected over a 19-year period from an iron tailings dam in Brazil. Clustering performance was assessed through visual inspection, stratigraphic consistency, and comparison with Ic-based profiles. k-means and MeanShift produced the most consistent stratigraphic segmentation, clearly delineating depositional layers, consolidated zones, and transitions linked to dam raising. In contrast, DBSCAN and Affinity Propagation either over-fragmented or failed to identify meaningful structures. The results demonstrate that clustering methods can reveal behavioral trends not detected by Ic alone, offering a complementary perspective for understanding depositional and mechanical evolution in tailings. Integrating clustering outputs with conventional geotechnical indices improves the interpretability of CPTu profiles, supporting more informed geomechanical modeling, dam monitoring, and design. The approach provides a replicable methodology for data-rich environments with high spatial and temporal variability. Full article
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18 pages, 11555 KiB  
Article
Impacts of Land Use and Hydrological Regime on the Spatiotemporal Distribution of Ecosystem Services in a Large Yangtze River-Connected Lake Region
by Ying Huang, Xinsheng Chen, Ying Zhuo and Lianlian Zhu
Water 2025, 17(15), 2337; https://doi.org/10.3390/w17152337 - 6 Aug 2025
Abstract
In river-connected lake regions, both land use and hydrological regime changes may affect the ecosystem services; however, few studies have attempted to elucidate their complex influences. In this study, the spatiotemporal dynamics of eight ecosystem services (crop production, aquatic production, water yield, soil [...] Read more.
In river-connected lake regions, both land use and hydrological regime changes may affect the ecosystem services; however, few studies have attempted to elucidate their complex influences. In this study, the spatiotemporal dynamics of eight ecosystem services (crop production, aquatic production, water yield, soil retention, flood regulation, water purification, net primary productivity, and habitat quality) were investigated through remote-sensing images and the InVEST model in the Dongting Lake Region during 2000–2020. Results revealed that crop and aquatic production increased significantly from 2000 to 2020, particularly in the northwestern and central regions, while soil retention and net primary productivity also improved. However, flood regulation, water purification, and habitat quality decreased, with the fastest decline in habitat quality occurring at the periphery of the Dongting Lake. Land-use types accounted for 63.3%, 53.8%, and 40.3% of spatial heterogeneity in habitat quality, flood regulation, and water purification, respectively. Land-use changes, particularly the expansion of construction land and the conversion of water bodies to cropland, led to a sharp decline in soil retention, flood regulation, water purification, net primary productivity, and habitat quality. In addition, crop production and aquatic production were higher in cultivated land and residential land, while the accompanying degradation of flood regulation, water purification, and habitat quality formed a “production-pollution-degradation” spatial coupling pattern. Furthermore, hydrological fluctuations further complicated these dynamics; wet years amplified agricultural outputs but intensified ecological degradation through spatial spillover effects. These findings underscore the need for integrated land-use and hydrological management strategies that balance human livelihoods with ecosystem resilience. Full article
(This article belongs to the Section Ecohydrology)
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19 pages, 3503 KiB  
Article
Discovery of Hub Genes Involved in Seed Development and Lipid Biosynthesis in Sea Buckthorn (Hippophae rhamnoides L.) Using UID Transcriptome Sequencing
by Siyang Zhao, Chengjiang Ruan, Alexey A. Dmitriev and Hyun Uk Kim
Plants 2025, 14(15), 2436; https://doi.org/10.3390/plants14152436 - 6 Aug 2025
Abstract
Sea buckthorn is a vital woody oil species valued for its role in soil conservation and its bioactive seed oil, which is rich in unsaturated fatty acids and other compounds. However, low seed oil content and small seed size are the main bottlenecks [...] Read more.
Sea buckthorn is a vital woody oil species valued for its role in soil conservation and its bioactive seed oil, which is rich in unsaturated fatty acids and other compounds. However, low seed oil content and small seed size are the main bottlenecks restricting the development and utilization of sea buckthorn. In this study, we tested the seed oil content and seed size of 12 sea buckthorn cultivars and identified the key genes and transcription factors involved in seed development and lipid biosynthesis via the integration of UID RNA-seq (Unique Identifiers, UID), WGCNA (weighted gene co-expression network analysis) and qRT-PCR (quantitative real-time PCR) analysis. The results revealed five cultivars (CY02, CY11, CY201309, CY18, CY21) with significantly higher oil contents and five cultivars (CY10, CY201309, CY18, CY21, CY27) with significantly heavier seeds. A total of 10,873 genes were significantly differentially expressed between the S1 and S2 seed developmental stages of the 12 cultivars. WGCNA was used to identify five modules related to seed oil content and seed weight/size, and 417 candidate genes were screened from these modules. Among them, multiple hub genes and transcription factors were identified; for instance, ATP synthase, ATP synthase subunit D and Acyl carrier protein 1 were related to seed development; plastid–lipid-associated protein, acyltransferase-like protein, and glycerol-3-phosphate 2-O-acyltransferase 6 were involved in lipid biosynthesis; and transcription factors DOF1.2, BHLH137 and ERF4 were associated with seed enlargement and development. These findings provide crucial insights into the genetic regulation of seed traits in sea buckthorn, offering targets for future breeding efforts aimed at improving oil yield and quality. Full article
(This article belongs to the Special Issue Molecular Regulation of Seed Development and Germination)
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17 pages, 1396 KiB  
Article
Dose-Dependent Effect of the Polyamine Spermine on Wheat Seed Germination, Mycelium Growth of Fusarium Seed-Borne Pathogens, and In Vivo Fusarium Root and Crown Rot Development
by Tsvetina Nikolova, Dessislava Todorova, Tzenko Vatchev, Zornitsa Stoyanova, Valya Lyubenova, Yordanka Taseva, Ivo Yanashkov and Iskren Sergiev
Agriculture 2025, 15(15), 1695; https://doi.org/10.3390/agriculture15151695 - 6 Aug 2025
Abstract
Wheat (Triticum aestivum L.) is a crucial global food crop. The intensive crop farming, monoculture cultivation, and impact of climate change affect the susceptibility of wheat cultivars to biotic stresses, mainly caused by soil fungal pathogens, especially those belonging to the genus [...] Read more.
Wheat (Triticum aestivum L.) is a crucial global food crop. The intensive crop farming, monoculture cultivation, and impact of climate change affect the susceptibility of wheat cultivars to biotic stresses, mainly caused by soil fungal pathogens, especially those belonging to the genus Fusarium. This situation threatens yield and grain quality through root and crown rot. While conventional chemical fungicides face resistance issues and environmental concerns, biological alternatives like seed priming with natural metabolites are gaining attention. Polyamines, including putrescine, spermidine, and spermine, are attractive priming agents influencing plant development and abiotic stress responses. Spermine in particular shows potential for in vitro antifungal activity against Fusarium. Optimising spermine concentration for seed priming is crucial to maximising protection against Fusarium infection while ensuring robust plant growth. In this research, we explored the potential of the polyamine spermine as a seed treatment to enhance wheat resilience, aiming to identify a sustainable alternative to synthetic fungicides. Our findings revealed that a six-hour seed soak in spermine solutions ranging from 0.5 to 5 mM did not delay germination or seedling growth. In fact, the 5 mM concentration significantly stimulated root weight and length. In complementary in vitro assays, we evaluated the antifungal activity of spermine (0.5–5 mM) against three Fusarium species. The results demonstrated complete inhibition of Fusarium culmorum growth at 5 mM spermine. A less significant effect on Fusarium graminearum and little to no impact on Fusarium oxysporum were found. The performed analysis revealed that the spermine had a fungistatic effect against the pathogen, retarding the mycelium growth of F. culmorum inoculated on the seed surface. A pot experiment with Bulgarian soft wheat cv. Sadovo-1 was carried out to estimate the effect of seed priming with spermine against infection with isolates of pathogenic fungus F. culmorum on plant growth and disease severity. Our results demonstrated that spermine resulted in a reduced distribution of F. culmorum and improved plant performance, as evidenced by the higher fresh weight and height of plants pre-treated with spermine. This research describes the efficacy of spermine seed priming as a novel strategy for managing Fusarium root and crown rot in wheat. Full article
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18 pages, 4216 KiB  
Article
Screening and Application of Highly Efficient Rhizobia for Leguminous Green Manure Astragalus sinicus in Lyophilized Inoculants and Seed Coating
by Ding-Yuan Xue, Wen-Feng Chen, Guo-Ping Yang, You-Guo Li and Jun-Jie Zhang
Plants 2025, 14(15), 2431; https://doi.org/10.3390/plants14152431 - 6 Aug 2025
Abstract
Astragalus sinicus, a key leguminous green manure widely cultivated in Southern China’s rice-based cropping systems, plays a pivotal role in sustainable agriculture by enhancing soil organic matter sequestration, improving rice yield, and elevating grain quality. The symbiotic nitrogen-fixing association between A. sinicus [...] Read more.
Astragalus sinicus, a key leguminous green manure widely cultivated in Southern China’s rice-based cropping systems, plays a pivotal role in sustainable agriculture by enhancing soil organic matter sequestration, improving rice yield, and elevating grain quality. The symbiotic nitrogen-fixing association between A. sinicus and its matching rhizobia is fundamental to its agronomic value; however, suboptimal inoculant efficiency and field application methodologies constrain its full potential. To address these limitations, we conducted a multi-phase study involving (1) rhizobial strain screening under controlled greenhouse conditions, (2) an optimized lyophilization protocol evaluating cryoprotectant (trehalose, skimmed milk powder and others), and (3) seed pelleting trails with rhizobial viability and nodulation assessments over different storage periods. Our results demonstrate that Mesorhizobium huakuii CCBAU 33470 exhibits a superior nitrogen-fixing efficacy, significantly enhancing key traits in A. sinicus, including leaf chlorophyll content, tiller number, and aboveground biomass. Lyophilized inoculants prepared with cryoprotectants (20% trehalose or 20% skimmed milk powder) maintained >90% bacterial viability for 60 days and markedly improved nodulation capacity relative to unprotected formulations. The optimized seed pellets sustained high rhizobial loads (5.5 × 103 cells/seed) with an undiminished viability after 15 days of storage and nodulation ability after 40 days of storage. This integrated approach of rhizobial selection, inoculant formulation, and seed coating overcomes cultivation bottlenecks, boosting symbiotic nitrogen fixation for A. sinicus cultivation. Full article
(This article belongs to the Topic New Challenges on Plant–Microbe Interactions)
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9280 KiB  
Proceeding Paper
Dynamical Modeling of Floods Using Surface Water Level Time Series
by Johan S. Duque, Jorge Zapata, Lucia de Leon, Alexander Gutierrez and Leonardo Santos
Eng. Proc. 2025, 101(1), 13; https://doi.org/10.3390/engproc2025101013 - 5 Aug 2025
Abstract
We present a dynamical systems approach to modeling nonlinear flood dynamics using 20 years of water level data from Durazno, Uruguay. Flood events are identified, and their periodicity and temporal distribution are analyzed in relation to rain gauge precipitation. Phase space reconstruction enables [...] Read more.
We present a dynamical systems approach to modeling nonlinear flood dynamics using 20 years of water level data from Durazno, Uruguay. Flood events are identified, and their periodicity and temporal distribution are analyzed in relation to rain gauge precipitation. Phase space reconstruction enables data-driven neural network modeling and quantification of the relationship between water level and soil moisture. Bifurcation diagrams define basin-specific flood thresholds, offering a mechanistic framework for improved flood forecasting and risk assessment. Full article
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17 pages, 1323 KiB  
Article
The Effect of Nitrogen Fertilizer Placement and Timing on Winter Wheat Grain Yield and Protein Concentration
by Brent Ballagh, Anna Ballagh, Jacob Bushong and Daryl Brian Arnall
Agronomy 2025, 15(8), 1890; https://doi.org/10.3390/agronomy15081890 - 5 Aug 2025
Abstract
Nitrogen (N) fertilizer management in winter wheat production faces challenges from volatilization losses and sub-optimal application strategies. This is particularly problematic in the Southern Great Plains, where environmental conditions during top-dressing periods favor N losses. This study evaluated the effects of a fertilizer [...] Read more.
Nitrogen (N) fertilizer management in winter wheat production faces challenges from volatilization losses and sub-optimal application strategies. This is particularly problematic in the Southern Great Plains, where environmental conditions during top-dressing periods favor N losses. This study evaluated the effects of a fertilizer placement method, enhanced-efficiency fertilizers, and application timing on grain yield and protein concentration (GPC) across six site-years in Oklahoma (2016–2018). Treatments included broadcast applications of untreated urea and SuperU® (urease/nitrification inhibitor-treated urea). These were compared with subsurface placement using single-disc and double-disc drilling systems, applied at 67 kg N ha−1 during January, February, or March. Subsurface placement increased the grain yield by 324–391 kg ha−1 compared to broadcast applications at sites with favorable soil conditions. However, responses varied significantly across environments. Enhanced-efficiency fertilizers showed limited advantages over untreated urea. Benefits were most pronounced during February applications under conditions favoring volatilization losses. Application timing effects were more consistent for GPC than for the yield. Later applications (February–March) increased GPC by 0.8–1.2% compared to January applications. Treatment efficacy was strongly influenced by soil pH, equipment performance, and post-application environmental conditions. This indicates that N management benefits are highly site-specific. These findings demonstrate that subsurface placement can improve nitrogen use efficiency (NUE) under appropriate conditions. However, success depends on matching application strategies to local soil and environmental factors rather than adopting universal recommendations. Full article
(This article belongs to the Special Issue Fertility Management for Higher Crop Productivity)
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19 pages, 642 KiB  
Article
Influence of Partial Vermicompost Tea Substitution for Mineral Nitrogen Fertilizers on Yield and Nutrient Content of Wheat Cultivars
by Hashim Abdel-Lattif and Mohamed Abbas
Crops 2025, 5(4), 51; https://doi.org/10.3390/crops5040051 - 5 Aug 2025
Abstract
Chemical fertilizers pose significant risks to both human health and the environment. To investigate the effect of substituting nitrogen fertilizer with vermicompost tea on wheat yield, shoot chemical constituents, and grain quality under clay-loam soil conditions, two field experiments were conducted at the [...] Read more.
Chemical fertilizers pose significant risks to both human health and the environment. To investigate the effect of substituting nitrogen fertilizer with vermicompost tea on wheat yield, shoot chemical constituents, and grain quality under clay-loam soil conditions, two field experiments were conducted at the Faculty of Agriculture, Cairo University, Egypt, during the winter seasons of 2021–2022 and 2022–2023. A split-plot design in randomized complete blocks with three replications was employed. Vermicompost tea was assigned to the main plots, while wheat cultivars were assigned to the subplots. The cultivars were evaluated under four treatments involving partial substitution of mineral nitrogen (recommended dose of nitrogen (RDN%, 190 kg N ha−1): a control (90% of RDN + 25 kg vermicompost tea), 80% of RDN + 37.5 kg vermicompost tea, and 70% of RDN + 50 kg vermicompost tea. Nitrogen fertilizer (RDN%) was applied at rates of 190 (control), 170 (90%), 150 (80%), and 130 (70%) kg N ha−1. The results indicated that partially substituting mineral nitrogen with vermicompost tea significantly increased grain weight/Ha, chlorophyll A, chlorophyll B, carotenoids, nitrogen, phosphorus (P), and potassium (K) content in shoots, as well as ash, crude protein, crude fiber, total sugar, and N, P, and K content in wheat grains. The grain weight/Ha of the Sakha-95, Giza-171, and Sads-14 cultivars increased by 38.6%, 33.5%, and 39.3%, respectively, when treated with 70% RDN + 50 kg vermicompost tea. The combination of the Sads-14 cultivar and 70% RDN + 50 kg vermicompost tea resulted in the highest values for grain weight/ha (9.43 tons ha−1), chlorophyll A (1.39 mg/g), chlorophyll B (1.04 mg/g), N (5.08%), P (1.63%), and P (2.43%) content in shoots. The same combination also improved ash (2.89%), crude fiber (2.84%), and K (6.05%) content in grains. In conclusion, the application of vermicompost tea in conjunction with chemical fertilizers offers a viable alternative to using chemical fertilizers alone, promoting sustainable agricultural practices and improving wheat production. It is recommended that mineral nitrogen fertilizer be partially replaced with vermicompost tea to enhance both the productivity and grain quality of wheat while minimizing environmental pollution. Full article
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17 pages, 7038 KiB  
Article
Polyploidy Induction of Wild Diploid Blueberry V. fuscatum
by Emily Walter, Paul M. Lyrene and Ye Chu
Horticulturae 2025, 11(8), 921; https://doi.org/10.3390/horticulturae11080921 (registering DOI) - 5 Aug 2025
Abstract
Diploid Vaccinium fuscatum is a wild blueberry species with a low chilling requirement, an evergreen growth habit, and soil adaptability to southeast US growing regions. Regardless of its potential to improve the abiotic and biotic resilience of cultivated blueberries, this species has rarely [...] Read more.
Diploid Vaccinium fuscatum is a wild blueberry species with a low chilling requirement, an evergreen growth habit, and soil adaptability to southeast US growing regions. Regardless of its potential to improve the abiotic and biotic resilience of cultivated blueberries, this species has rarely been used for blueberry breeding. One hurdle is the ploidy barrier between diploid V. fuscatum and tetraploid cultivated highbush blueberries. To overcome the ploidy barrier, vegetative shoots micro-propagated from one genotype of V. fuscatum, selected because it grew vigorously in vitro and two southern highbush cultivars, ‘Emerald’ and ‘Rebel,’ were treated with colchicine. While shoot regeneration was severely repressed in ‘Emerald’ and ‘Rebel,’ shoot production from the V. fuscatum clone was not compromised at either 500 µM or 5000 µM colchicine concentrations. Due to the high number of shoots produced in vitro via the V. fuscatum clone shoots of this clone that had an enlarged stem diameter in vitro were subjected to flow cytometer analysis to screen for induced polyploidy. Sixteen synthetic tetraploid V. fuscatum, one synthetic octoploid ‘Emerald,’ and three synthetic octoploid ‘Rebel’ were identified. Growth rates of the polyploid-induced mutants were reduced compared to their respective wildtype controls. The leaf width and length of synthetic tetraploid V. fuscatum and synthetic octoploid ‘Emerald’ was increased compared to the wildtypes, whereas the leaf width and length of synthetic octoploid ‘Rebel’ were reduced compared to the wildtype controls. Significant increases in stem thickness and stomata guard cell length were found in the polyploidy-induced mutant lines compared to the wildtypes. In the meantime, stomata density was reduced in the mutant lines. These morphological changes may improve drought tolerance and photosynthesis in these mutant lines. Synthetic tetraploid V. fuscatum can be used for interspecific hybridization with highbush blueberries to expand the genetic base of cultivated blueberries. Full article
(This article belongs to the Section Propagation and Seeds)
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18 pages, 2479 KiB  
Article
Seasonality of Arbuscular Mycorrhizal Fungal Diversity and Glomalin in Sodic Soils of Grasslands Under Contrasting Grazing Intensities
by Ileana García, Karla Cáceres-Mago and Alejandra Gabriela Becerra
Soil Syst. 2025, 9(3), 87; https://doi.org/10.3390/soilsystems9030087 (registering DOI) - 5 Aug 2025
Abstract
Arbuscular mycorrhizal fungi (AMF) taxa, glomalin protein, and hyphal density are potential indicators of soil functionality of temperate grasslands in marginal environments subject to grazing over the years. This study evaluated how the AMF community composition, glomalin protein, and hyphal density vary in [...] Read more.
Arbuscular mycorrhizal fungi (AMF) taxa, glomalin protein, and hyphal density are potential indicators of soil functionality of temperate grasslands in marginal environments subject to grazing over the years. This study evaluated how the AMF community composition, glomalin protein, and hyphal density vary in response to grazing intensity (low or high) and seasonality (spring and autumn) in sodic soils of Argentinian temperate grasslands. The AMF community was dominated by Glomeraceae species. Funneliformis geosporus and Glomus brohultii were the most abundant in both seasons and all grasslands. No AMF species were associated with a particular grazing intensity. However, Entrophospora etunicata, Glomus fuegianum, Septoglomus constrictum, and Acaulospora sp. occurred only in spring, and no species were exclusive to autumn. Hyphal density was highest in grasslands with low grazing intensity and can be considered an indicator of soil functionality. Glomalin protein was the highest in spring in all grasslands. The lower grazing intensity in grasslands with poor livestock control showed no changes in AMF diversity. The AMF community showed high adaptation to soil conditions, indicating high resilience. We concluded that longer periods of controlled grazing management are needed to improve soil conditions and, consequently, change the AMF species composition. Full article
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18 pages, 2357 KiB  
Article
Nitrogen Fertilizer Reduction in Rice–Eel Co-Culture System Improves the Soil Microbial Diversity and Its Functional Stability
by Mengqian Ma, Weiguang Lv, Yu Huang, Juanqin Zhang, Shuangxi Li, Naling Bai, Haiyun Zhang, Xianpu Zhu, Chenglong Xu and Hanlin Zhang
Plants 2025, 14(15), 2425; https://doi.org/10.3390/plants14152425 - 5 Aug 2025
Abstract
The ecological rice–eel co-culture system is not only beneficial for enhancing productivity and sustainability in agriculture but also plays a crucial role in promoting environmental health. In the present study, based on the long-term positioning trial of the rice–eel co-culture system that began [...] Read more.
The ecological rice–eel co-culture system is not only beneficial for enhancing productivity and sustainability in agriculture but also plays a crucial role in promoting environmental health. In the present study, based on the long-term positioning trial of the rice–eel co-culture system that began in 2016 and was sampled in 2023, the effects of reduced nitrogen fertilizer application on soil physico-chemical properties and the bacterial community were investigated. Treatments included a conventional regular fertilization treatment (RT), rice–eel co-culture system regular fertilization (IT), and nitrogen-reduction 10%, 30%, and 50% fertilization treatments (IT90, IT70, and IT50). Our research demonstrated the following: (1) Compared to RT, IT significantly increased soil water-stable macroaggregates (R0.25), mean weight diameter (MWD), geometric mean diameter (GMD), and available phosphorus content, with the increases of 15.66%, 25.49%, 36.00%, and 18.42%, respectively. Among the nitrogen-reduction fertilization treatments, IT90 showed the most significant effect. Compared to IT, IT90 significantly increased R0.25, MWD, GMD, and available nitrogen content, with increases of 4.4%, 7.81%, 8.82%, and 28.89%, respectively. (2) Compared to RT, at the phylum level, the diversity of Chloroflexi was significantly increased under IT and IT50, and the diversity of Gemmatimonadota was significantly increased under IT90, IT70, and IT50. The diversity of Acidobacteriota was significantly higher in IT90 and IT70 compared to IT. It was shown that the rice–eel co-culture system and nitrogen fertilizer reduction could effectively improve the degradation capacity of organic matter and promote soil nitrogen cycling. In addition, redundancy analysis (RDA) identified total phosphorus, total nitrogen, and available nitrogen (p = 0.007) as the three most important environmental factors driving changes in the bacterial community. (3) The functional prediction analysis of soil microbiota showed that, compared to RT, the diversity of pathways related to biosynthesis (carbohydrate biosynthesis and cell structure biosynthesis) and metabolism (L-glutamate and L-glutamine biosynthesis) was significantly higher under IT70, IT90, IT, and IT50 (in descending order). However, the diversity of pathways associated with degradation/utilization/assimilation (secondary metabolite degradation and amine and polyamine degradation) was significantly lower under all the rice–eel co-culture treatments. In conclusion, the rice–eel co-culture system improved soil physicochemical properties and the soil microbial environment compared with conventional planting, and the best soil improvement was achieved with 10% less N fertilizer application. Full article
(This article belongs to the Special Issue Chemical Properties of Soils and its Impact on Plant Growth)
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17 pages, 4589 KiB  
Article
Evaluation of Slope Stability and Landslide Prevention in a Closed Open-Pit Mine Used for Water Storage
by Pengjiao Zhang, Yuan Gao, Yachao Liu and Tianhong Yang
Appl. Sci. 2025, 15(15), 8659; https://doi.org/10.3390/app15158659 (registering DOI) - 5 Aug 2025
Abstract
To study and quantify the impact of water storage on lake slope stability after the closure of an open-pit mine, we targeted slope control measures by large-scale parallel computing methods and strength reduction theory. This was based on a three-dimensional refined numerical model [...] Read more.
To study and quantify the impact of water storage on lake slope stability after the closure of an open-pit mine, we targeted slope control measures by large-scale parallel computing methods and strength reduction theory. This was based on a three-dimensional refined numerical model to simulate the evolution of slope stability under different water storage levels and backfilling management conditions, and to quantitatively assess the risk of slope instability through the spatial distribution of stability coefficients. This study shows that during the impoundment process, the slope stability has a nonlinear decreasing trend due to the decrease in effective stress caused by the increase in pore water pressure. When the water storage was at 0 m, the instability range is the largest, and the surface range is nearly 200 m from the edge of the pit; when the water level continued to rise to 50 m, the hydrostatic pressure of the pit lake water on the slope support effect began to appear, and the stability was improved, but there is still a wide range of unstable areas at the bottom. In view of the unstable area of the steep slope with soft rock in the north slope during the process of water storage, the management scheme of backfilling the whole bottom to −150 m was proposed, and the slope protection and pressure footing were formed by discharging the soil to −40 m in steps to improve the anti-slip ability of the slope. Full article
(This article belongs to the Special Issue Advances in Slope Stability and Rock Fracture Mechanisms)
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