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36 pages, 23123 KB  
Article
Evaluating Environmental and Crop Factors Affecting Drone-Mounted GPR Performance in Agricultural Fields
by Milad Vahidi and Sanaz Shafian
Sensors 2026, 26(6), 1873; https://doi.org/10.3390/s26061873 - 16 Mar 2026
Abstract
Drone-mounted ground-penetrating radar (GPR) systems offer new opportunities for integrating subsurface characterization into remote sensing workflows. However, the interaction between flight parameters, surface conditions, and vegetation characteristics remains poorly understood. This study investigates the impact of flight altitude, surface topography, crop presence, and [...] Read more.
Drone-mounted ground-penetrating radar (GPR) systems offer new opportunities for integrating subsurface characterization into remote sensing workflows. However, the interaction between flight parameters, surface conditions, and vegetation characteristics remains poorly understood. This study investigates the impact of flight altitude, surface topography, crop presence, and canopy water content on the stability and interpretability of GPR signals collected using a drone. Field experiments were conducted under controlled conditions using agricultural plots with variable canopy cover and soil moisture regimes. Radargrams were processed to evaluate signal amplitude, reflection continuity, and attenuation patterns in relation to terrain slope and vegetation structure derived from co-registered RGB drone imagery. The results reveal that lower flight altitudes and smoother surfaces yield higher signal coherence and greater subsurface penetration, while increased canopy water content and biomass reduce signal strength and clarity. Integrating drone-based GPR observations with surface spectral and thermal data improved discrimination between soil and vegetation-induced signal distortions. The findings highlight the potential of drone–GPR systems as a complementary layer in a multi-sensor remote sensing framework for precision agriculture, environmental monitoring, and 3D soil mapping. Full article
(This article belongs to the Section Sensors and Robotics)
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21 pages, 6566 KB  
Article
GmRWP-RK1 Enhances Salt Tolerance by Modulating Antioxidant Defense, Ion Homeostasis and Stress-Responsive Pathways in Soybean
by Lu Liu, Qianyue Bai, Min Xu, Qi Zhang, Yuhong Gai, Naveed Ahmad, Piwu Wang, Zhuo Zhang, Nooral Amin and Wei Jian
Plants 2026, 15(6), 912; https://doi.org/10.3390/plants15060912 - 16 Mar 2026
Abstract
Soil salinity is rapidly spreading across agricultural regions and has become one of the most critical constraints on soybean growth, yield, and sustainable production. Despite the central role of transcription factors (TFs) in coordinating plant responses to abiotic stresses, the molecular mechanisms by [...] Read more.
Soil salinity is rapidly spreading across agricultural regions and has become one of the most critical constraints on soybean growth, yield, and sustainable production. Despite the central role of transcription factors (TFs) in coordinating plant responses to abiotic stresses, the molecular mechanisms by which RWP-RK domain-containing TFs regulate salt-tolerant responses in soybean remain poorly understood. Our previous genome-wide characterization identified 28 RWP-RK TFs in soybean exhibiting abiotic stress-responsive expression, yet their biological functions under salt stress have not been experimentally validated. Here, we investigated a 981-bp GmRWP-RK1 encoding region and demonstrated its regulatory role in enhancing salt tolerance by activating antioxidant defence, Na+/K+ homeostasis, and transcriptional control of salt-responsive genes using a cross-species overexpression approach. The two Arabidopsis lines (OE1 & OE4) overexpressing GmRWP-RK1 demonstrated significantly improved salt tolerance, as evidenced by ~18% greater survival and enhanced germination compared to non-transgenic plants under salinity stress. This phenotype was supported by stronger antioxidant protection, as indicated by elevated proline levels, reduced MDA accumulation, and increased SOD and POD activities. At the molecular level, the transgenic lines also showed up-regulated expression of key stress-responsive genes (AtACS10, AtSUMO1, AtGBF1), confirming the regulatory influence of GmRWP-RK1 on salt-adaptation pathways. Consistent with the Arabidopsis results, GmRWP-RK1 overexpression in soybean hairy roots also led to improved salt-stress tolerance by accumulating significantly reduced ROS contents (27.38% lower H2O2 and 33.98% lower O2), and maintained a balanced Na+/K+ ratio compared to that of non-transgenic hairy roots under salinity. Furthermore, GmRWP-RK1-overexpressing transgenic soybean hairy roots showed increased expression of stress-responsive genes, especially GmATG-5, GmOLP-1, and GmOLP-2. Overall, our results support a possible role of GmRWP-RK1 in soybean salt tolerance and provide a foundation for future functional and breeding-oriented studies. Full article
(This article belongs to the Special Issue Abiotic Stress Responses in Plants—Second Edition)
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27 pages, 9493 KB  
Article
Long-Term Land Use/Land Cover Change and Climate-Driven Projection of Soil Organic Carbon Stocks and Sequestration Using the RothC Model in the Northern Nile Delta, Egypt
by Noura Bakr, Sahar A. Shahin, Ahmed A. Afifi and Elsayed F. Essa
Sustainability 2026, 18(6), 2884; https://doi.org/10.3390/su18062884 - 15 Mar 2026
Abstract
Soil organic carbon (SOC) is a major component of the global carbon cycle. This study aimed to: (i) monitor five decades’ land use/land cover (LULC) changes in the northern Nile delta using Landsat imagery; (ii) quantify baseline SOC stocks (SOCs) in 2021; (iii) [...] Read more.
Soil organic carbon (SOC) is a major component of the global carbon cycle. This study aimed to: (i) monitor five decades’ land use/land cover (LULC) changes in the northern Nile delta using Landsat imagery; (ii) quantify baseline SOC stocks (SOCs) in 2021; (iii) project SOCs and potential SOC sequestration (PSOCS) to 2100 under four SSP2-4.5 climate scenarios using RothC model; and (iv) evaluate uncertainty in SOCs and PSOCS projections using the Monte Carlo approach. Sixty soil samples were collected during the winter and summer seasons of 2018/2019 (30 per season). Agricultural land expanded from 12% in 1972 to 35% in 2021, while fish farms, established in the 1990s, accounted for 24% of the area by 2021. SOCs varied across LULC types and seasons. Between 13 and 28% of agricultural land exceeding 7 Mg C ha−1 in summer and winter, respectively. Barren land and sabkha were characterized by low SOCs (<3 Mg C ha−1). Model predictions indicate that mean SOCs will increase from 5.83 (2021) to 6.16 (mid-century), followed by a decline to 5.96 Mg C ha−1 by 2100. Estimated PSOCS range from 0.13 to 0.32 Mg C ha−1. Monte Carlo uncertainty analysis yielded median SOCs between 6.01 and 6.27 Mg C ha−1 and median PSOCS between 0.18 and 0.44 Mg C ha−1, reflecting moderate projection uncertainty. Full article
(This article belongs to the Section Soil Conservation and Sustainability)
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29 pages, 16838 KB  
Article
Spatiotemporal Evolution of Drought and Its Multi-Factor Driving Mechanisms in Xinjiang During 1981–2020
by Xuchuang Yu, Siguo Liu, Anni Deng, Runsen Li, Xiaotao Hu, Ping’an Jiang and Ning Yao
Agriculture 2026, 16(6), 669; https://doi.org/10.3390/agriculture16060669 - 15 Mar 2026
Abstract
Drought is a highly destructive natural disaster that inflicts severe economic losses. Its formation mechanisms are complex, yet existing studies have often focused on single driving factors, leaving the synergistic effects of multiple factors insufficiently explored. Based on multi-source data from Xinjiang spanning [...] Read more.
Drought is a highly destructive natural disaster that inflicts severe economic losses. Its formation mechanisms are complex, yet existing studies have often focused on single driving factors, leaving the synergistic effects of multiple factors insufficiently explored. Based on multi-source data from Xinjiang spanning 1981–2020, this study systematically examined the combined impacts of atmospheric circulation, underlying surface conditions, and human activities on drought, using the multi-temporal-scale Standardized Precipitation Evapotranspiration Index (SPEI) and Standardized Soil Moisture Index (SSI), along with partial correlation analysis, spatial autocorrelation, and principal component analysis. The results show that Xinjiang experienced a pronounced drying trend over the past 40 years, with the seasonal SPEI and SSI both exhibiting significant declines. Drought intensity was higher in northern Xinjiang than in the south. Correlations between drought indices and circulation indices, such as Atlantic Multidecadal Oscillation (AMO), were relatively weak, indicating a limited regulatory influence of large-scale circulation on regional drought under the dual constraints of topography and an inland setting. Among underlying surface factors, slope significantly influenced drought spatial patterns. Mountainous areas and basin interiors showed positive spatial correlations, characterized respectively by high–high clustering (high slope and high drought index) and low–low clustering (low slope and low drought index). In contrast, basin margins exhibited low–high clustering (low slope surrounded by high drought index), reflecting negative spatial correlation. Aspect showed no significant effect. Vegetation cover displayed clear seasonal coupling with drought, with strong negative correlations in spring due to intensified water stress. Human activities also played a prominent role. Since the mid-1990s, the expansion of built-up land and increased agricultural water use have shifted drought–land use relationships toward low–high clustering (low drought index surrounded by high land-use intensity) in southern Xinjiang oases, and toward low–low clustering (low drought index and low land-use intensity) in eastern Xinjiang. Meanwhile, ecological restoration projects promoted a transition from low–high to high–high clustering (high drought index and high land-use intensity) in some areas, alleviating local drying trends. Principal component analysis further revealed a shift in the dominant driver: land-use change was the primary factor before 2005, whereas vegetation cover became the key driver thereafter. By clarifying the mechanisms underlying multi-factor interactions in drought in Xinjiang, this study provides scientific support for integrated water resource management, ecological conservation, and climate adaptation strategies in arid regions. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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19 pages, 9427 KB  
Article
Research on Creep Characteristics of Dredged Fill Soil in Humen Port Considering the Effect of Temperature
by Xiaodi Xu, Qiunan Chen and Chen Zhang
Appl. Sci. 2026, 16(6), 2820; https://doi.org/10.3390/app16062820 - 15 Mar 2026
Abstract
Dredged Fill Soil, as a primary foundation material in reclamation projects, exhibits complex physical and mechanical properties, characterized by a high plasticity index, high water content, low density, high compressibility, large void ratio, and low bearing capacity. Its creep behavior is highly sensitive [...] Read more.
Dredged Fill Soil, as a primary foundation material in reclamation projects, exhibits complex physical and mechanical properties, characterized by a high plasticity index, high water content, low density, high compressibility, large void ratio, and low bearing capacity. Its creep behavior is highly sensitive to temperature changes. This study systematically investigates the temperature-dependent creep behavior of reclaimed soil from Humen Port through laboratory experiments, theoretical modeling, and experimental validation. Triaxial creep tests conducted at different temperatures (5 °C, 15 °C, 25 °C, 35 °C) show that increasing temperature significantly exacerbates creep deformation: under undrained conditions, creep strain at 35 °C is nearly 300% higher than at 5 °C, while drainage reduces the strain by approximately 29.3%. Based on these results, a Burgers-type creep constitutive model considering temperature effects is developed, incorporating the impact of temperature on viscosity and elastic modulus. The model’s predictions show good agreement with the experimental results (15 °C: R2 = 0.9788; 35 °C: R2 = 0.9890), confirming the model’s validity. The research findings provide theoretical and practical references for the long-term stability evaluation and engineering design of reclaimed foundations in complex marine environments. Full article
(This article belongs to the Special Issue Effects of Temperature on Geotechnical Engineering)
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23 pages, 3050 KB  
Article
Polyethylene Mulch Emissions Differentially Impact the Soil Metabolome and Microbial Community in Field Pea (Pisum sativum L.) Cultivation
by Emoke Dalma Kovacs, Nguyen Khoi Nghia and Melinda Haydee Kovacs
J. Xenobiot. 2026, 16(2), 49; https://doi.org/10.3390/jox16020049 - 15 Mar 2026
Abstract
Background and Objectives: Polyethylene (PE) mulching enhances crop productivity through microclimate optimization but introduces synthetic polymer-derived compounds into agricultural soils. Despite widespread use, biochemical and microbial impacts of PE mulch emissions remain poorly understood. This study investigated the impact of PE mulch emissions [...] Read more.
Background and Objectives: Polyethylene (PE) mulching enhances crop productivity through microclimate optimization but introduces synthetic polymer-derived compounds into agricultural soils. Despite widespread use, biochemical and microbial impacts of PE mulch emissions remain poorly understood. This study investigated the impact of PE mulch emissions on soil metabolomes and microbial communities during field pea (Pisum sativum L.) cultivation. Methods: A 75-day field experiment compared PE-mulched and non-mulched soils across five temporal sampling points (T0–T4). Headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry was used to identify PE-derived organic compounds in mulched soils. Microbial community structure was assessed through the phospholipids derived fatty acids (PLFA) approach, whereas mass spectrometric untargeted metabolomics was used to characterize the soil biochemical profiles. Results: Analysis identified 18 PE-derived organic compounds (n-alkanes, phthalates, and additives) in the mulched soils. PE mulching significantly increased bacterial abundance (anaerobic bacteria, actinomycetes, and aerobic bacteria) but suppressed all functional fungal guilds, particularly saprotrophic fungi (30% reduction) and arbuscular mycorrhizal symbionts. PE-derived organic compounds were associated primarily with the first RDA axis (RDA1), which alone explained 44.6% of the metabolome variance. These compounds presented strong positive correlations with organic nitrogen compounds and lipids and negative correlations with benzenoids and nucleotides. Pathway analysis revealed perturbations in energy metabolism, lipid metabolism, and xenobiotic degradation pathways. Conclusions: PE mulch emissions differentially shift soil microbial communities and metabolic networks, with bacterial proliferation contrasting with fungal suppression. These findings highlight the complex trade-offs between agronomic benefits and soil biological impacts, emphasizing the need for sustainable mulching alternatives. Full article
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17 pages, 1415 KB  
Article
The Application of Saline–Alkali-Tolerant Growth-Promoting Endophytic Bacteria for Enhancing the Saline–Alkali Tolerance of Alfalfa
by Muhammad Rahman Ali Shah, Lu Tang, Hao Zhou, Huiying Zheng, Yimeng Shi and Changhong Guo
Biology 2026, 15(6), 474; https://doi.org/10.3390/biology15060474 - 15 Mar 2026
Abstract
Saline–alkali stress is a severe abiotic factor that limits plant growth and development. Endophytic bacteria can improve plant tolerance to such stress through various mechanism, including osmoregulatory substance accumulation and antioxidant enzyme activity. In this study, four saline–alkali-tolerant endophytic strains, designated SYM-2, SYM-4, [...] Read more.
Saline–alkali stress is a severe abiotic factor that limits plant growth and development. Endophytic bacteria can improve plant tolerance to such stress through various mechanism, including osmoregulatory substance accumulation and antioxidant enzyme activity. In this study, four saline–alkali-tolerant endophytic strains, designated SYM-2, SYM-4, SYM-9, and SYM-15, were isolated from the roots of alfalfa grown in saline–alkali soil. Though 16S rDNA sequencing, morphological observations, and physiological–biochemical characterization, the strains were identified as closely related to Bacillus cereus, B. thuringiensis, B. halotolerans, and Pantoea agglomerans, respectively. These strains demonstrated the ability to produce 1-aminocyclopropane-1-carboxylate (ACC) deaminase, siderophores, and indole-3-acetic acid (IAA), as well as solubilizing phosphorus. Under saline–alkali conditions, inoculation with these strains significantly increased alfalfa growth parameters. Plant height increased by 4.07–33.90% and root length by 7.49–27.94%, and fresh and dry weight (both above and below ground) increased compared with the control. Strain SYM-15 showed the highest promoting effects, increasing plant height by 33.90%, root length by 27.94% and shoot dry weight by 59.26%. Additionally, root activity increased by 11.23–40%, proline content by 19.09–129–87%, and soluble protein by 7.71–42.49%, and the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were significantly elevated across treatments. At the same time, inoculation reduced the levels of hydrogen peroxide (H2O2), superoxide anion (O2), and malondialdehyde (MDA). Compared with the control and other treatments, including SYM-9, the peroxidase activity and superoxide dismutase activity of alfalfa significantly increased after the SYM-15 treatment, while hydrogen peroxide content, phosphorus content, and neutral detergent fiber and acid detergent fiber contents decreased (p < 0.05). Therefore, SYM-15 plays an important role in promoting growth and represents a promising, high-quality strain resource for the large-scale development of microbials aimed at improving alfalfa tolerance under saline–alkali conditions. Full article
(This article belongs to the Collection Plant Growth-Promoting Bacteria: Mechanisms and Applications)
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33 pages, 8401 KB  
Article
Soil Pore Architecture and Hydraulic Functioning of Native Forest and Sugarcane Systems with and Without Cover Crop Intercropping Revealed by X-Ray Computed Tomography
by Gabriel Oladele Awe, Ademir de Oliveira Ferreira, Brivaldo Gomes de Almeida, Williams Ramos da Silva, Antonio Celso Dantas Antonino and José Miguel Reichert
Forests 2026, 17(3), 365; https://doi.org/10.3390/f17030365 - 14 Mar 2026
Abstract
Soil pore architecture and hydraulic functioning strongly regulate water flow and retention. However, despite the growing application of X-ray computed tomography (X-ray CT) in soil science, its application in characterizing the pore system and hydraulic functioning of native forest soils converted to sugarcane [...] Read more.
Soil pore architecture and hydraulic functioning strongly regulate water flow and retention. However, despite the growing application of X-ray computed tomography (X-ray CT) in soil science, its application in characterizing the pore system and hydraulic functioning of native forest soils converted to sugarcane production systems in northeast Brazil is still poorly known. This study therefore quantified the soil structure, pore system, and hydraulic functioning of a native forest (NF) and an adjacent sugarcane field receiving vinasse and managed without intercropping (sole sugarcane (SG)) and with Brachiaria ruziziensis intercropping (SG + Bra intercrop) in northeastern Brazil, using conventional soil physical measurements and X-ray CT, in three soil layers (0–10, 10–20, and 20–40 cm). Soil physical and hydraulic properties, as well as soil water retention, were quantified. The native forest soil exhibited a uniformly sandy texture across all depths, whereas sugarcane systems ranged from loam to sandy textures in surface layers due to long-term management. Soil organic matter and total nitrogen in the 0–10 cm layer were approximately 75 and 65% higher, respectively, in sole Sole SG and SG + Bra intercrop than in NF. Soil bulk density increased with depth under sugarcane, reaching values about 10%–13% higher than NF in the 20–40 cm layer. Saturated hydraulic conductivity in the surface layer was higher in the NF, approximately five to nine times greater than in sole SG and SG + Bra intercrop, respectively. Conventional water retention analysis showed that sole SG and SG + Bra intercrop had greater total porosity (0.49–0.55 m3 m−3), microporosity (0.26–0.36 m3 m−3), field capacity (0.19–0.33 m3 m−3), and plant available water (0.09–0.15 m3 m−3) in the upper 20 cm compared with the NF (≤0.10 m3 m−3 available water). In contrast, X-ray CT revealed higher macroporosity (0.20–0.23 mm3 mm−3) and pore connectivity in the NF across all depths, with predominantly complex, inclined to near-horizontal pores and low anisotropy. Intercropping sugarcane with Brachiaria did not significantly alter (p > 0.05) bulk density, hydraulic conductivity, or CT-derived pore connectivity relative to sole sugarcane. The degree of anisotropy and fractal dimension derived from X-ray CT were significantly correlated (p < 0.05) with conventionally measured hydraulic properties. The X-ray computed tomography proved effective in linking pore-scale architecture to soil hydraulic functioning, providing insights beyond conventional measurements. The short-term inclusion of Brachiaria as a cover crop at 10 kg seed ha−1 did not result in significant improvements in soil pore structure, indicating that longer-term adoption and/or higher planting densities may be required to induce measurable changes in pore system architecture and soil hydraulic functioning. Full article
(This article belongs to the Special Issue Forest Soil Stability in Response to Global Change Scenarios)
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23 pages, 2269 KB  
Article
A Comparative Study on the Sustainable Remediation of Arsenic Pollution in Water and Soil Using Iron-Modified and Cerium-Modified Biochar
by Siyuan Wang, Xiaoxian Yuan, Shifeng Li, Shiji Bie, Yang Zhou, Shuzheng Guo and Zhipu Wang
Sustainability 2026, 18(6), 2873; https://doi.org/10.3390/su18062873 - 14 Mar 2026
Abstract
Arsenic (As) pollution has become a global concern, and the search for effective and sustainable As remediation methods has attracted much attention. Sustainable and cost-effective technologies for As remediation are essential to protect public health. This study aligns with the United Nations Sustainable [...] Read more.
Arsenic (As) pollution has become a global concern, and the search for effective and sustainable As remediation methods has attracted much attention. Sustainable and cost-effective technologies for As remediation are essential to protect public health. This study aligns with the United Nations Sustainable Development Goals (SDGs), specifically SDG 6 (Clean Water and Sanitation) and SDG 12 (Responsible Consumption and Production), by transforming agricultural waste into value-added biochar for environmental remediation. Currently, studies on the remediation of As pollution using iron-modified biochar (Fe-BC) and cerium-modified biochar (Ce-BC) have demonstrated promising application potential. Although there is an established research foundation regarding their remediation performance and mechanisms, comparative studies evaluating their performance and mechanisms under unified experimental conditions remain limited. As in this study, Fe-BC and Ce-BC were prepared and systematically investigated. The As remediation performance and mechanisms of the two biochars were compared and analyzed through material characterization, aqueous adsorption experiments, and soil remediation assessments. The results showed that the specific surface areas of Fe-BC and Ce-BC were 94.380 m2·g−1 and 36.388 m2·g−1, respectively, both higher than that of the original biochar (BC). The Langmuir and Freundlich models adequately fitted the As adsorption processes of all three materials. Fe-BC and Ce-BC exhibited a tendency toward monolayer adsorption for As(III). The Freundlich distribution coefficient KF of Fe-BC was 0.1604, which was higher than that of BC and Ce-BC, indicating superior adsorption performance for As(III). In the pot experiment, when Fe-BC and Ce-BC were applied at 5%, the As content in ryegrass decreased by 78.38% and 77.15%, respectively. Fe-BC reduced the available As content in soil by 63.1% and decreased As accumulation in ryegrass by 78.38%. The reduction in available As content achieved by Fe-BC was greater than that achieved by Ce-BC. Fe(III) oxides supported on Fe-BC immobilized As through complexation and precipitation mechanisms. Fe0 and Fe3O4 in the materials altered the redox potential of the local microenvironment, affecting the transformation and stabilization of As species. Ce-BC primarily oxidized As(III) to As(V), and Ce4+ facilitated the formation of CeAsO4 precipitates due to its high redox potential. Full article
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18 pages, 673 KB  
Article
Short-Term Trace Element Distribution Following Application of Sargassum-Based Liquid Biofertilizer in a Soil–Plant–Tomato Fruit System
by Yaset Rodríguez-Rodríguez, Máximo Elías Reynoso Ortega, Pamela Tejada-Tejada, Gustavo Gandini, Luis Enrique Rodríguez de Francisco and Ulises Javier Jáuregui-Haza
Plants 2026, 15(6), 901; https://doi.org/10.3390/plants15060901 - 14 Mar 2026
Abstract
The recurrent influx of pelagic Sargassum spp. along Caribbean coastlines poses a significant environmental challenge while offering potential as a resource-recovery agricultural input. However, agricultural reuse of Sargassum biomass raises concerns regarding salinity and trace-metal distribution within the soil–plant–food continuum. This study evaluated [...] Read more.
The recurrent influx of pelagic Sargassum spp. along Caribbean coastlines poses a significant environmental challenge while offering potential as a resource-recovery agricultural input. However, agricultural reuse of Sargassum biomass raises concerns regarding salinity and trace-metal distribution within the soil–plant–food continuum. This study evaluated the short-term elemental response to a Sargassum-Based Liquid Biofertilizer (SBLB) produced via controlled anaerobic fermentation, using tomato (Solanum lycopersicum L.) grown under greenhouse conditions. Raw biomass, fermented biofertilizer, irrigation water, soils, vegetative tissues, and fruits were chemically characterized. Elemental concentrations were quantified by ICP–OES and ICP-MS and treatment effects were analyzed using one-way and two-way ANOVA (p < 0.05). Anaerobic fermentation resulted in lower measured concentrations of sodium, arsenic, and selected trace elements in the liquid fraction relative to raw biomass. SBLB application increased soil macronutrient availability (N, P, K, Ca, Mg), while soil trace-metal concentrations remained within international reference ranges during the experimental period. Metals of concern (As, Cd, Pb, Ni, Cr) showed no detectable short-term enrichment in soils, vegetative tissues, or fruits relative to controls. In tomato fruits, arsenic, cadmium, and lead were below the limit of quantification across all treatments. Within the experimental timeframe, SBLB application was not associated with detectable trace-element accumulation in the soil–plant system. Long-term field studies and detailed soil physicochemical characterization are required to evaluate cumulative effects under repeated applications. Full article
(This article belongs to the Topic Plant-Soil Interactions, 2nd Volume)
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22 pages, 2143 KB  
Article
Biochar Derived from Black Liquor as a Soil Amendment: Effects on Soil Quality, Growth Parameters, Chlorophyll and Mineral Content of Barley
by Anca Maria Zaharioiu, Oana Maria Tanislav, Marius Constantinescu, Antoaneta Roman, Felicia Bucura, Violeta-Carolina Niculescu, Simona Oancea and Florian Marin
Agriculture 2026, 16(6), 659; https://doi.org/10.3390/agriculture16060659 - 13 Mar 2026
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Abstract
This study investigated the application of biochar obtained from black liquor, a residue generated during the Kraft pulping process in the paper industry, as a sustainable soil amendment in barley (Hordeum vulgare L.) cultivation. The biochar was produced through controlled pyrolysis at [...] Read more.
This study investigated the application of biochar obtained from black liquor, a residue generated during the Kraft pulping process in the paper industry, as a sustainable soil amendment in barley (Hordeum vulgare L.) cultivation. The biochar was produced through controlled pyrolysis at 450 °C and subsequently characterized with respect to elemental composition, porosity, specific surface area, and chemical stability, confirming its suitability for agricultural use. The experiment comprised three treatments: unamended soil (control), soil supplemented with 3% biochar, and soil fertilized with NPK, all conducted under controlled growth conditions. The results showed that biochar significantly improved key soil fertility indicators, increasing cation exchange capacity from 11 to 19 cmol(+)/kg and soil organic matter from 2.1% to 2.6%. Mineral nitrogen availability increased from 7.0 mg/kg to 10.5 mg/kg in the biochar treatment compared with the control. At the plant level, biochar enhanced early barley growth, with plant height increasing from 25 cm to 27 cm and chlorophyll content rising from 32.35 SPAD units to 39 SPAD units. Although NPK fertilization produced slightly higher immediate growth responses, biochar contributed to improved soil chemical properties and nutrient retention. Overall, the results suggest that black liquor-derived biochar shows potential as a complementary soil amendment under controlled conditions. Full article
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28 pages, 1916 KB  
Article
Valorization of Corncob and Khat Waste into Biochar via Decentralized Multi-Purpose Pyrolysis Stoves
by Tarekegn Limore Binchebo, Krzysztof Pikoń, Venkata Ramayya Ancha, Teka Tesfaye Mengesha, Solomon Kebede Asefa, Defar Getahun Gizachew and Mamo Abawalo
Energies 2026, 19(6), 1461; https://doi.org/10.3390/en19061461 - 13 Mar 2026
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Abstract
The escalating global waste crisis necessitates sustainable valorization strategies, with biochar production emerging as a promising solution for converting organic residues into a carbon-rich material. This study evaluated biochar derived from corncob and khat waste pyrolyzed using allo-thermal and auto-thermal multi-purpose stoves. Biochar [...] Read more.
The escalating global waste crisis necessitates sustainable valorization strategies, with biochar production emerging as a promising solution for converting organic residues into a carbon-rich material. This study evaluated biochar derived from corncob and khat waste pyrolyzed using allo-thermal and auto-thermal multi-purpose stoves. Biochar was fractionated into four particle sizes (>2 mm, 1–2 mm, 0.6–1 mm, and <0.6 mm) and characterized for ash content, pH, and electrical conductivity (EC). Results demonstrated that the auto-thermal stove, operating at higher temperatures (up to 800 °C), consistently produced biochar with greater ash content (khat: 12–19%; corncob: 11–14%), alkaline pH (9.2–10.0), and significantly higher EC compared to the allo-thermal stove (maximum 350 °C). EC values ranged from 0.38 mS/cm (coarse allo-thermal corncob) to 6.6 mS/cm (fine auto-thermal khat), with auto-thermal biochar exhibiting EC values 5–10 times higher than their allo-thermal counterparts. khat waste consistently yielded biochar with higher ash content, pH, and EC than corncob, reflecting its richer mineral composition. Particle size fractionation revealed that pH and EC increased progressively with decreasing particle size across all treatments, with the finest fraction (<0.6 mm) showing the highest values. For auto-thermal khat, EC increased from 2.43 mS/cm (>2 mm) to 6.6 mS/cm (<0.6 mm). This study demonstrates that decentralized biochar production using multi-purpose stoves can yield materials with tunable properties, and that khat waste—an underutilized regional resource—shows particular promise for producing high-ash, high-EC biochar suitable for acidic soil amendment. Full article
(This article belongs to the Special Issue Emission Control and Sustainable Energy)
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11 pages, 2221 KB  
Article
Molecular Identification and Characterization of Peribacillus simplex LT4 Isolated from the Roots of Baby Maize (Zea mays L.)
by Chuong Van Nguyen and Tri Le Kim Tran
Nitrogen 2026, 7(1), 28; https://doi.org/10.3390/nitrogen7010028 - 13 Mar 2026
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Abstract
Rhizosphere nitrogen-fixing bacteria play a critical role in sustainable crop production by enhancing nitrogen availability and improving soil fertility. This study aimed to isolate and characterize native rhizospheric nitrogen-fixing bacteria (NRNFB) associated with baby maize (Zea mays L.) roots and evaluate their [...] Read more.
Rhizosphere nitrogen-fixing bacteria play a critical role in sustainable crop production by enhancing nitrogen availability and improving soil fertility. This study aimed to isolate and characterize native rhizospheric nitrogen-fixing bacteria (NRNFB) associated with baby maize (Zea mays L.) roots and evaluate their nitrogen-fixing potential. Thirty root samples were collected, and ten bacterial isolates (V1–V10) were obtained using selective media. Morphological, biochemical, and physiological analyses identified strain V3 as the most promising candidate, exhibiting strong growth on nitrogen-free Burk medium and high oxidase, catalase, and urea hydrolysis activities. The strain demonstrated broad environmental tolerance, including salinity up to 4% NaCl, temperatures ranging from 15 to 45 °C, and pH values between 5.0 and 8.0. Molecular identification based on 16S rRNA gene sequencing revealed 100% sequence similarity with Peribacillus simplex LT4 (strain LT4). Nitrogenase activity analysis showed a peak during the exponential growth phase, accompanied by increased nitrogen accumulation in the culture medium, confirming active biological nitrogen fixation. These findings highlight the physiological adaptability and functional efficiency of strain LT4, supporting its potential development as a biofertilizer for sustainable maize production systems. Full article
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27 pages, 1194 KB  
Review
Lifecycle Risks and Environmental Fate of Titanium Dioxide Nanoparticles in Automotive Coatings
by Emma Landskroner and Candace Su-Jung Tsai
Environments 2026, 13(3), 156; https://doi.org/10.3390/environments13030156 - 13 Mar 2026
Viewed by 106
Abstract
Titanium dioxide nanoparticles (TiO2 NPs) are incorporated into automotive coatings to enhance durability, corrosion, UV resistance, and, in some formulations, photocatalytic self-cleaning. While the toxicology of pristine TiO2 is well studied, the behavior of TiO2 NPs embedded in polymer matrices [...] Read more.
Titanium dioxide nanoparticles (TiO2 NPs) are incorporated into automotive coatings to enhance durability, corrosion, UV resistance, and, in some formulations, photocatalytic self-cleaning. While the toxicology of pristine TiO2 is well studied, the behavior of TiO2 NPs embedded in polymer matrices and subjected to real-world aging, maintenance, and removal remains poorly characterized. This narrative review synthesizes 24 publications spanning the lifecycle of TiO2 nano-enabled automotive coatings, from synthesis and formulation through application, in-service weathering, repair, refinishing, and end-of-life environmental fate. Upstream properties, such as coating functionality and performance, have been examined as determinants of later-life release, exposure, and fate. Across studies, dispersion state, interfacial compatibility, and surface modification—together with transformations such as agglomeration, photocatalysis, weathering, and eco-corona formation—shape particle stability, release, exposure relevance, and toxicological risk. Evidence indicates that sanding and accelerated weathering predominantly generate matrix-associated, polymer-fragment-dominated aerosols rather than pristine TiO2 NPs, while NP-specific exposure measurements during spray application remain limited. Hazard data suggest matrix embedding may attenuate, but does not eliminate, biological responses relative to pure particles. Wastewater treatment plants and biosolids have been shown to act as sinks with potential for soil accumulation following sludge application. Regulatory frameworks rarely account for aging, transformation, and release, stressing the need for synchronized testing of aged materials and nano-specific exposure metrics to support safer-by-design coatings and risk governance. Full article
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23 pages, 8944 KB  
Article
Impact of Clogging on the Infiltration Performance of Porous Asphalt Mixtures Under a GIS–USLE-Based Multiscale Assessment of Peri-Urban Sediment Loads: A Case Study in Boyacá, Colombia
by Andres Silva-Balaguera, Julian Villate-Corredor, Jessica Betancourt-Gonzalez, Karen Fuquene-Saenz and Luis Ángel Sañudo-Fontaneda
Water 2026, 18(6), 669; https://doi.org/10.3390/w18060669 - 13 Mar 2026
Viewed by 160
Abstract
Clogging is the main mechanism that deteriorates the hydraulic functionality of permeable pavements, particularly in porous asphalt mixtures (PAM). This study evaluated the hydraulic impact of sediments from three peri-urban micro-watersheds in the Boyacá region of Colombia on the infiltration capacity of PAM. [...] Read more.
Clogging is the main mechanism that deteriorates the hydraulic functionality of permeable pavements, particularly in porous asphalt mixtures (PAM). This study evaluated the hydraulic impact of sediments from three peri-urban micro-watersheds in the Boyacá region of Colombia on the infiltration capacity of PAM. Road infrastructure and drainage conditions were analysed using orthophotos and field inspections to identify geomorphological factors that favour sediment transport toward the roadway. Annual erosion rates were estimated using the Universal Soil Loss Equation (USLE), and sediments were characterized both within the watersheds and at their outlet onto the road. Hydraulic performance was assessed through laboratory tests using a Falling Head Permeameter, complemented by field infiltration measurements with a Modified Cantabrian Infiltrometer (0.25 m2). Results showed erosion rates of up to 7.9 t/ha·year and infiltration losses above 90% under clogged conditions. A partial hydraulic recovery of around 40% was observed after maintenance, particularly when sediments exhibited a higher sand fraction. These findings demonstrate that combining USLE-based erosion modelling with controlled hydraulic testing provides a robust framework for evaluating clogging risks in peri-urban roads and offers new evidence on the hydraulic behaviour of PAM exposed to non-urban sediments in the design and maintenance of sustainable pavements. Full article
(This article belongs to the Special Issue Urban Drainage Systems and Stormwater Management, 2nd Edition)
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