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41 pages, 580 KiB  
Review
The Alarming Effects of Per- and Polyfluoroalkyl Substances (PFAS) on One Health and Interconnections with Food-Producing Animals in Circular and Sustainable Agri-Food Systems
by Gerald C. Shurson
Sustainability 2025, 17(15), 6957; https://doi.org/10.3390/su17156957 - 31 Jul 2025
Viewed by 187
Abstract
Per- and polyfluoroalkyl substances (PFAS) are synthetically produced chemicals that are causing a major One Health crisis. These “forever chemicals” are widely distributed globally in air, water, and soil, and because they are highly mobile and extremely difficult to degrade in the environment. [...] Read more.
Per- and polyfluoroalkyl substances (PFAS) are synthetically produced chemicals that are causing a major One Health crisis. These “forever chemicals” are widely distributed globally in air, water, and soil, and because they are highly mobile and extremely difficult to degrade in the environment. They cause additional health concerns in a circular bioeconomy and food system that recycles and reuses by-products and numerous types of waste materials. Uptake of PFAS by plants and food-producing animals ultimately leads to the consumption of PFAS-contaminated food that is associated with numerous adverse health and developmental effects in humans. Contaminated meat, milk, and eggs are some of the main sources of human PFAS exposure. Although there is no safe level of PFAS exposure, maximum tolerable PFAS consumption guidelines have been established for some countries. However, there is no international PFAS monitoring system, and there are no standardized international guidelines and mechanisms to prevent the consumption of PFAS-contaminated foods. Urgent action is needed to stop PFAS production except for critical uses, implementing effective water-purification treatments, preventing spreading sewage sludge on land and pastures used to produce food, and requiring marketers and manufacturers to use packaging that is free of PFAS. Full article
29 pages, 3259 KiB  
Review
The Role of the Environment (Water, Air, Soil) in the Emergence and Dissemination of Antimicrobial Resistance: A One Health Perspective
by Asma Sassi, Nosiba S. Basher, Hassina Kirat, Sameh Meradji, Nasir Adam Ibrahim, Takfarinas Idres and Abdelaziz Touati
Antibiotics 2025, 14(8), 764; https://doi.org/10.3390/antibiotics14080764 - 29 Jul 2025
Viewed by 439
Abstract
Antimicrobial resistance (AMR) has emerged as a planetary health emergency, driven not only by the clinical misuse of antibiotics but also by diverse environmental dissemination pathways. This review critically examines the role of environmental compartments—water, soil, and air—as dynamic reservoirs and transmission routes [...] Read more.
Antimicrobial resistance (AMR) has emerged as a planetary health emergency, driven not only by the clinical misuse of antibiotics but also by diverse environmental dissemination pathways. This review critically examines the role of environmental compartments—water, soil, and air—as dynamic reservoirs and transmission routes for antibiotic-resistant bacteria (ARB) and resistance genes (ARGs). Recent metagenomic, epidemiological, and mechanistic evidence demonstrates that anthropogenic pressures—including pharmaceutical effluents, agricultural runoff, untreated sewage, and airborne emissions—amplify resistance evolution and interspecies gene transfer via horizontal gene transfer mechanisms, biofilms, and mobile genetic elements. Importantly, it is not only highly polluted rivers such as the Ganges that contribute to the spread of AMR; even low concentrations of antibiotics and their metabolites, formed during or after treatment, can significantly promote the selection and dissemination of resistance. Environmental hotspots such as European agricultural soils and airborne particulate zones near wastewater treatment plants further illustrate the complexity and global scope of pollution-driven AMR. The synergistic roles of co-selective agents, including heavy metals, disinfectants, and microplastics, are highlighted for their impact in exacerbating resistance gene propagation across ecological and geographical boundaries. The efficacy and limitations of current mitigation strategies, including advanced wastewater treatments, thermophilic composting, biosensor-based surveillance, and emerging regulatory frameworks, are evaluated. By integrating a One Health perspective, this review underscores the imperative of including environmental considerations in global AMR containment policies and proposes a multidisciplinary roadmap to mitigate resistance spread across interconnected human, animal, and environmental domains. Full article
(This article belongs to the Special Issue The Spread of Antibiotic Resistance in Natural Environments)
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21 pages, 1897 KiB  
Article
Simulation of Conventional WWTPs Acting as Mediators in H2/CO2 Conversion into Methane
by Rubén González and Xiomar Gómez
Environments 2025, 12(7), 245; https://doi.org/10.3390/environments12070245 - 16 Jul 2025
Viewed by 509
Abstract
CO2-biomethanation was studied in the present manuscript by considering the direct injection of hydrogen into a conventional anaerobic digester treating sewage sludge within a simulated wastewater treatment plant (WWTP). The plant was simulated using the Python 3.12.4 software, and a Monte [...] Read more.
CO2-biomethanation was studied in the present manuscript by considering the direct injection of hydrogen into a conventional anaerobic digester treating sewage sludge within a simulated wastewater treatment plant (WWTP). The plant was simulated using the Python 3.12.4 software, and a Monte Carlo simulation was conducted to account for the high variability in the organic content of the wastewater and the methane potential of the sludge. Two modes of operation were studied. The first mode involves the use of an anaerobic digester to upgrade biogas, and the second mode considers using the digester as a CO2 utilization unit, transforming captured CO2. Upgrading biogas and utilizing the extra methane to generate electricity within the same plant leads to a negative economic balance (first scenario). A hydrogen injection of 1 L of H2/Lr d (volumetric H2 injection per liter of reactor per day) was required to transform the CO2 present in the biogas into methane. The benefits associated with this approach resulted in lower savings regarding heat recovery from the electrolyzer, increased electricity production, and an additional oxygen supply for the waste-activated sludge treatment system. Increasing the injection rate to values of 5 and 30 L of H2/Lr d was also studied by considering the operation of the digester under thermophilic conditions. The latter assumptions benefited from the better economy of scale associated with larger installations. They allowed for enough savings to be obtained in terms of the fuel demand for sludge drying, in addition to the previous categories analyzed in the biogas upgrading case. However, the current electricity price makes the proposal unfeasible unless a lower price is set for hydrogen generation. A standard electricity price of 7.6 c€/kWh was assumed for the analysis, but the specific operation of producing hydrogen required a price below 3.0 c€/kWh to achieve profitability. Full article
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17 pages, 6479 KiB  
Article
Operation of a Zero-Discharge Evapotranspiration Tank for Blackwater Disposal in a Rural Quilombola Household, Brazil
by Adivânia Cardoso da Silva, Adriana Duneya Diaz Carrillo and Paulo Sérgio Scalize
Water 2025, 17(14), 2098; https://doi.org/10.3390/w17142098 - 14 Jul 2025
Viewed by 453
Abstract
Decentralized sanitation in rural areas urgently requires accessible and nature-based solutions to achieve Sustainable Development Goal 6 (clean water and sanitation for all). However, monitoring studies of such ecotechnologies in disperse communities remain limited. This study evaluated the performance of an evapotranspiration tank [...] Read more.
Decentralized sanitation in rural areas urgently requires accessible and nature-based solutions to achieve Sustainable Development Goal 6 (clean water and sanitation for all). However, monitoring studies of such ecotechnologies in disperse communities remain limited. This study evaluated the performance of an evapotranspiration tank (TEvap), designed with community participation, for the treatment of domestic sewage in a rural Quilombola household in the Brazilian Cerrado. The system (total area of 8.1 m2, with about 1.0 m2 per inhabitant) was monitored for 218 days, covering the rainy season and the plants’ establishment phase. After 51 days, the TEvap reached operational equilibrium, maintaining a zero-discharge regime, and after 218 days, 92.3% of the total system inlet volumes (i.e., 37.47 in 40.58 m3) were removed through evapotranspiration and uptake by cultivated plants (Musa spp.). Statistical analyses revealed correlations that were moderate to strong, and weak between the blackwater level and relative humidity (Pearson correlation coefficient, r = 0.75), temperature (r = −0.66), and per capita blackwater contribution (r = 0.28), highlighting the influence of climatic conditions on system efficiency. These results confirm the TEvap as a promising, low-maintenance, and climate-resilient technology for decentralized domestic sewage treatment in vulnerable rural communities, with the potential to support sanitation policy goals and promote public health. Full article
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14 pages, 2951 KiB  
Article
Utilization of Sewage Sludge in the Sustainable Manufacturing of Ceramic Bricks
by Kairat Ospanov, Dariusz Andraka, Elmira Kuldeeva and Issatay Munussov
Sustainability 2025, 17(14), 6431; https://doi.org/10.3390/su17146431 - 14 Jul 2025
Viewed by 340
Abstract
The storage of sewage sludge in landfills is still the primary method of their disposal in many countries. Therefore, finding sustainable solutions for the reuse of this waste is an important issue to be addressed. This paper presents the results of research on [...] Read more.
The storage of sewage sludge in landfills is still the primary method of their disposal in many countries. Therefore, finding sustainable solutions for the reuse of this waste is an important issue to be addressed. This paper presents the results of research on processing sewage sludge generated at the wastewater treatment plants of Almaty, Kazakhstan, for use in the manufacturing of ceramic bricks. The chemical composition of the sewage sludge was determined, showing the presence of 35.7% silicon dioxide, 7.3% aluminum oxide, 11.2% iron oxide, and 10.6% calcium oxide. Experimental studies established that adding sewage sludge to clay reduces the compressive strength of ceramic bricks while increasing their flexural strength. Furthermore, as the proportion of sewage sludge in the clay mixture increases, the average density of the ceramic bricks decreases, while water absorption increases. It was also found that ceramic bricks made from a mixture of 90% clay and 10% sewage sludge demonstrated the best physico-mechanical properties. This composition showed increased flexural strength and a simultaneous reduction in the average density by 15.4%, indicating the improved structural quality of the manufactured bricks. Full article
(This article belongs to the Special Issue Sustainable Advancements in Construction Materials)
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22 pages, 2944 KiB  
Article
From Wastewater to Soil Amendment: A Case Study on Sewage Sludge Composting and the Agricultural Application of the Compost
by Csilla Almási, Zoltán Veres, Ibolya Demeter, Viktória Orosz, Tímea Tóth, Mostafa M. Mansour, István Henzsel, Zsolt Bogdányi, Tamás András Szegi and Marianna Makádi
Water 2025, 17(13), 2026; https://doi.org/10.3390/w17132026 - 5 Jul 2025
Viewed by 583
Abstract
The treatment of wastewater and the utilization of the by-products of these processes are an important part of the circular economy. The sewage sludge, a result of wastewater treatment, could be used as a material for plant nutrient supply and/or soil-improving products. The [...] Read more.
The treatment of wastewater and the utilization of the by-products of these processes are an important part of the circular economy. The sewage sludge, a result of wastewater treatment, could be used as a material for plant nutrient supply and/or soil-improving products. The city of Nyíregyháza, Hungary, with 120,000 citizens, has a well-planned water treatment plant operated by Nyírségvíz Ltd., which, in cooperation with the Research Institute of Nyíregyháza, developed a municipal sewage sludge compost (SSC). The closed loop of sewage water treatment and the agricultural utilization of its by-product has been developed and managed. The compost product called Nyírkomposzt was planned for acidic sandy soils. Beyond the agronomic benefits, the sustainable and environmentally sound utilization of SSC reduces sewage sludge disposal. This active involvement of a water utility company demonstrates the potential of cross-sectoral cooperation in solving environmental problems. The quality of the compost fits the Hungarian legislation. To study the effects of 0, 9, 18, and 27 t ha−1 doses of compost on acidic sandy soil, a long-term small plot experiment was started in 2003. The cumulative effects of the regular (every third year, last treatment before sampling in 2021) application of the SSC showed positive changes in basic soil properties, depending on the doses used. Increasing values were found in the case of pH from 4.5 to 6, plant available P2O5 from 240 to 690 ppm, and plant available K2O from 180 to 200 ppm. The plant-available zinc and copper content also increased. Soil organic matter and total N content stabilized at around 0.9% and 0.08%, respectively. The grain yields of winter rye also increased in both investigated years. The yields of 18 t ha−1 treatment were about two times higher compared to the control, but only in 2022 was the difference significant. Our findings underscore the potential of well-planned SSC applications to improve the fertility of ploughed, acidic sandy soil, taking into account the theory of the circular economy by utilizing wastes and decreasing landfilling. Full article
(This article belongs to the Special Issue Treatment and Resource Utilization of Urban Sewage Sludge)
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20 pages, 2601 KiB  
Article
Waste as a Source of Fuel and Developments in Hydrogen Storage: Applied Cases in Spain and Their Future Potential
by Juan Pous de la Flor, María-Pilar Martínez-Hernando, Roberto Paredes, Enrique Garcia-Franco, Juan Pous Cabello and Marcelo F. Ortega
Appl. Sci. 2025, 15(13), 7514; https://doi.org/10.3390/app15137514 - 4 Jul 2025
Viewed by 365
Abstract
The integration of renewable energy with circular economy strategies offers effective pathways to reduce greenhouse gas emissions while enhancing local energy independence. This study analyses three real-world projects implemented in Spain that exemplify this synergy. LIFE Smart Agromobility converts pig manure into biomethane [...] Read more.
The integration of renewable energy with circular economy strategies offers effective pathways to reduce greenhouse gas emissions while enhancing local energy independence. This study analyses three real-world projects implemented in Spain that exemplify this synergy. LIFE Smart Agromobility converts pig manure into biomethane to power farm vehicles, using anaerobic digestion and microalgae-based upgrading systems. Smart Met Value refines biogas from a wastewater treatment plant in Guadalajara to produce high-purity biomethane for the municipal fleet, demonstrating the viability of energy recovery from sewage sludge. The UNDERGY project addresses green hydrogen storage by repurposing a depleted natural gas reservoir, showing geochemical and geomechanical feasibility for seasonal underground hydrogen storage. Each project utilises regionally available resources to produce clean fuels—biomethane or hydrogen—while mitigating methane and CO2 emissions. Results show significant energy recovery potential: biomethane production can replace a substantial portion of fossil fuel use in rural and urban settings, while hydrogen storage provides a scalable solution for surplus renewable energy. These applied cases demonstrate not only the technical feasibility but also the socio-economic benefits of integrating waste valorisation and energy transition technologies. Together, they represent replicable models for sustainable development and energy resilience across Europe and beyond. Full article
(This article belongs to the Section Energy Science and Technology)
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16 pages, 7027 KiB  
Article
Quantitative Assessment of Seasonal and Land-Use Impacts on Coastal Urban Sewage Systems with Seawater Intrusion Vulnerability Analysis
by Yanhong Ge, Jiachong Lin, Qidong Yin, Sheng Huang, Yingchao Lin and Kai He
Water 2025, 17(13), 1939; https://doi.org/10.3390/w17131939 - 28 Jun 2025
Viewed by 352
Abstract
Based on the sewage pipe network system in the service area of Qianshan-Gongbei Plant in Zhuhai City, the characteristics of water quality and quantity were analyzed, and the common problems were diagnosed. Through the establishment of a hydraulic-water quality model, the flow state [...] Read more.
Based on the sewage pipe network system in the service area of Qianshan-Gongbei Plant in Zhuhai City, the characteristics of water quality and quantity were analyzed, and the common problems were diagnosed. Through the establishment of a hydraulic-water quality model, the flow state of sewage in the pipe network is simulated, and the actual data is checked. It is found that there are significant differences in the quantity and quality of sewage pipe network systems in different seasons and land use types, and there is an obvious seawater backflow phenomenon in coastal areas. To solve these problems, this paper puts forward a series of optimization suggestions to improve the operation efficiency of sewage treatment plants and the reliability of urban drainage systems. Full article
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17 pages, 1866 KiB  
Article
Risk Management in the Analysis of Failures of Protective Coatings in Municipal Sewage Treatment Plant Tanks
by Janusz Banera, Marek Maj and Ahmad H. Musa
Buildings 2025, 15(13), 2254; https://doi.org/10.3390/buildings15132254 - 26 Jun 2025
Viewed by 267
Abstract
Polyurea failures in reinforced concrete tanks, such as swimming pools and sewage treatment plants, require a thorough analysis of the causes of failures during renovation. Urban agglomerations are increasingly relying on these facilities for maintaining city functioning, and the increasing concentration of pollutants [...] Read more.
Polyurea failures in reinforced concrete tanks, such as swimming pools and sewage treatment plants, require a thorough analysis of the causes of failures during renovation. Urban agglomerations are increasingly relying on these facilities for maintaining city functioning, and the increasing concentration of pollutants in these facilities necessitates urgent repairs due to frequent failures. More thorough analysis should be given to repeated failures on the same object or “twin” objects within a short period, causing high renovation costs and long shutdowns. The causes of failures can be found not only as a result of insufficient knowledge but also in a limited analysis of the entire project from the assumption phase to completion. The article analyzed water and sewage tanks on which failures of applied polyurea coatings occurred many times. The posteriori uses of the risk management analysis with the assessment of the impact and probability of occurrence of the planned activities that failed allows it to be applied a priori and treated as a necessary analysis. For this purpose, in selected repairs, those activities that had the greatest impact on failure and a relatively high probability of occurrence during implementation were distinguished from the entire project. Based on the risk management analysis, it was shown that the basic cause of the failure was the poor knowledge and insufficient experience of the entities performing the repairs, and the errors that occurred could be minimized by conducting good diagnostics of the facility, selecting professional designers and contractors, and constant monitoring of each important activity. Full article
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24 pages, 1615 KiB  
Article
Comparative Effects of Non-Composted and Composted Sewage Sludge from Wastewater Treatment Plants on the Physiological and Antioxidative Responses of Maize
by Dávid Kaczur, Makoena Joyce Moloi, Seyed Mohammad Nasir Mousavi and Brigitta Tóth
Plants 2025, 14(13), 1955; https://doi.org/10.3390/plants14131955 - 26 Jun 2025
Viewed by 450
Abstract
This study evaluated the physiological and antioxidative responses of maize to non-composted (NCSS) and composted sewage sludge (CSS) from Debrecen and Kecskemét, applied at 25%, 50%, and 75% (m/m) concentrations. Measurements were taken 21 and 35 days after sowing (DAS). Debrecen NCSS significantly [...] Read more.
This study evaluated the physiological and antioxidative responses of maize to non-composted (NCSS) and composted sewage sludge (CSS) from Debrecen and Kecskemét, applied at 25%, 50%, and 75% (m/m) concentrations. Measurements were taken 21 and 35 days after sowing (DAS). Debrecen NCSS significantly enhanced plant height at all concentrations and at both sampling times, while higher doses of Kecskemét NCSS reduced growth by 35 DAS. Chlorophyll-a, chlorophyll-b, and carotenoid contents were notably enhanced by Kecskemét treatments, especially at lower concentrations, whereas Debrecen treatments showed less effect. Photosystem II (PSII) efficiency parameters varied with origin: Kecskemét NCSS notably increased minimal fluorescence (Fo), while Debrecen CSS occasionally reduced maximum fluorescence (Fm) and variable fluorescence (Fv) at 75% dose (21 DAS). The superoxide dismutase activity (SOD) was significantly elevated in Kecskemét treatments—by 101%, 148% and 149% at 25%, 50% and 75% CSS applications. Correlation analysis further highlighted that NCSS treatments often showed negative associations between plant height and chlorophyll content but positive correlations with antioxidant activity. In contrast, CSS treatments promoted balanced physiological responses. The results support the importance of sludge origin and application rate and suggest that composted sludge can be a safe, sustainable amendment when managed appropriately. Full article
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16 pages, 1827 KiB  
Article
Tropical Wetlands as Nature-Based Solutions to Remove Nutrient and Organic Inputs from Stormwater Discharge and Wastewater Effluent in Urban Environments
by Flavia Byekwaso, Gabriele Weigelhofer, Rose Kaggwa, Frank Kansiime, Guenter Langergraber and Thomas Hein
Water 2025, 17(12), 1821; https://doi.org/10.3390/w17121821 - 18 Jun 2025
Viewed by 549
Abstract
Natural wetlands are critical water quality regulators, especially in developing tropical countries. The Lubigi wetland is a large urban wetland in Kampala, the largest city in Uganda in Africa. We studied whether stormwater discharge and wastewater effluent from a nearby stormwater channel and [...] Read more.
Natural wetlands are critical water quality regulators, especially in developing tropical countries. The Lubigi wetland is a large urban wetland in Kampala, the largest city in Uganda in Africa. We studied whether stormwater discharge and wastewater effluent from a nearby stormwater channel and a sewage treatment plant in the western part of the city were cleaned as they flowed through the wetland. Despite the significant pollution, the wetland removed ammonium-nitrogen, orthophosphate, and particulate nutrients during both seasons, achieving removal rates ranging from 50 to 60% for orthophosphate but only 20–40% for ammonium-nitrogen. Overall, seasonal differences in loads and retention rates of nutrient and organic matter inputs were minimal. Interestingly, the wetland mostly released nitrate and nitrite during water passage through the wetland, most likely due to the mineralization of organic nitrogen and agricultural run-off during rainy events in the wet season. However, the limited capacity of the sewage treatment plant and untreated stormwater discharge from the Nsooba main channel reduced the wetland’s ability to clean water. The insufficient carrying capacity of the treatment plant and the release of untreated sewage into the wetland significantly impact the self-purification capacity of the Lubigi wetland. Thus, the concept of Nature-Based Solutions is ineffective if the wetland systems are overloaded. Full article
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17 pages, 2430 KiB  
Article
Analysis of Hotspots in the Field of Sulfonamides Treatment: A Bibliometric Review
by Jian Wang, Xinyao Liu, Feng Qian and Jie Su
Water 2025, 17(12), 1792; https://doi.org/10.3390/w17121792 - 15 Jun 2025
Viewed by 371
Abstract
Sulfonamide drugs (SAs) are a class of emerging contaminants widely present in water environments, which has gradually attracted attention from scholars worldwide. Based on the Web of Science core collection database, this study employs bibliometric methods and visualization tools, such as CiteSpace, Bibliometrix, [...] Read more.
Sulfonamide drugs (SAs) are a class of emerging contaminants widely present in water environments, which has gradually attracted attention from scholars worldwide. Based on the Web of Science core collection database, this study employs bibliometric methods and visualization tools, such as CiteSpace, Bibliometrix, and VOSviewer, to systematically analyze the literature on the treatment of SAs from 2004 to 2024, exploring the research status, hotspots, and development trends in this field. The results indicate that research on SAs in the past 20 years can be categorized into three stages: initial exploration (2004–2008), slow development (2009–2016), and in-depth research (2017–2024), with an overall increasing trend in number of publications. China and the United States have published the most articles on SAs, with 2266 and 592 articles respectively, and the collaborative ties between the two countries are the strongest. The Chinese Academy of Sciences is the most prolific institution, having published 348 articles. Science of the Total Environment is the journal with the highest publication volume. Among the many SAs, sulfamethoxazole has garnered the greatest research interest, and its primary entry route into the water environment is through the discharge of sewage treatment plants. The research focus has gradually shifted from the source analysis of SAs in the environment to seeking efficient methods for removing SAs. Future research should prioritize investigations into antibiotic-resistant bacteria and antibiotic resistance genes associated with SAs. Full article
(This article belongs to the Section Wastewater Treatment and Reuse)
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21 pages, 1011 KiB  
Article
Novel Bacterial Strains for Nonylphenol Removal in Water and Sewage Sludge: Insights from Gene Expression and Toxicity
by Alba Lara-Moreno, Inés Aguilar-Romero, Fernando Madrid, Jaime Villaverde, Jorge D. Carlier, Juan Luís Santos, Esteban Alonso and Esmeralda Morillo
Appl. Sci. 2025, 15(12), 6408; https://doi.org/10.3390/app15126408 - 6 Jun 2025
Viewed by 393
Abstract
4-Nonylphenols (4-NPs) are persistent endocrine disruptors frequently found in wastewater treatment plant (WWTP) effluents and sewage sludge. This study evaluated the ability of eight bacterial strains that were isolated from sewage sludge to degrade 4-n-NP in an aqueous solution. Bacillus safensis CN12, Shewanella [...] Read more.
4-Nonylphenols (4-NPs) are persistent endocrine disruptors frequently found in wastewater treatment plant (WWTP) effluents and sewage sludge. This study evaluated the ability of eight bacterial strains that were isolated from sewage sludge to degrade 4-n-NP in an aqueous solution. Bacillus safensis CN12, Shewanella putrefaciens CN17, and Alcaligenes faecalis CN8 showed the highest degradation rates, removing 100%, 75%, and 74% of 4-n-NP (10 mg L⁻1), with DT50 values of 0.90, 8.9, and 10.4 days, respectively. Despite the reduction in 4-n-NP concentrations, ecotoxicity assays revealed that the resulting transformation products (TPs) were more toxic than the parent compound. To investigate the potential degradation mechanisms, in silico and gene expression analyses were conducted on B. safensis CN12, revealing a significant upregulation of the multicopper oxidase gene, cotA (7.25-fold), and the ring-cleaving dioxygenase gene, mhqO (13.9-fold). Although the CN12 strain showed potential for mineralization based on gene expression studies, this was not observed in the aqueous solution. However, when 4-n-NP was adsorbed on sludge and treated with CN12 in the presence of hydroxypropyl-β-cyclodextrin (HPBCD) as a bioavailability enhancer, mineralization reached up to 33%, indicating a synergistic effect with the native sludge microbiota. Full article
(This article belongs to the Section Applied Microbiology)
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22 pages, 4149 KiB  
Article
Profiling of Bacterial Communities of Hospital Wastewater Reveals Clinically Relevant Genera and Antimicrobial Resistance Genes
by Clemente Cruz-Cruz, Javier Gaytán-Cervantes, Carolina González-Torres, Andres Emmanuel Nolasco-Rojas, Miguel Ángel Loyola-Cruz, Laura Delgado-Balbuena, Josué Delgado-Balbuena, Marianela Paredes-Mendoza, María Concepción Tamayo-Ordóñez, Yahaira de Jesús Tamayo-Ordoñez, Emilio Mariano Durán-Manuel, Araceli Rojas-Bernabé, Carlos Alberto Jiménez-Zamarripa, Oscar Sosa-Hernández, Omar Agni García-Hernández, Esther Ocharan-Hernández, Paola Berenice Zárate-Segura, Elizabeth González-Terreros, Daniel Alejandro Ramírez-Villanueva, Claudia Camelia Calzada-Mendoza and Juan Manuel Bello-Lópezadd Show full author list remove Hide full author list
Microorganisms 2025, 13(6), 1316; https://doi.org/10.3390/microorganisms13061316 - 5 Jun 2025
Viewed by 1202
Abstract
In Mexico, hospital wastewater (HWW) is a source of chemical and microbiological contamination, and it is released into the municipal sewage system without prior treatment. This water may contain pathogenic bacteria and antimicrobial resistance genes, which represent a risk to Public Health and [...] Read more.
In Mexico, hospital wastewater (HWW) is a source of chemical and microbiological contamination, and it is released into the municipal sewage system without prior treatment. This water may contain pathogenic bacteria and antimicrobial resistance genes, which represent a risk to Public Health and the environment. So far, there are no studies that analyse this problem comprehensively, relating bacterial population structures, chemical contaminants, and seasonality. The aim of this work was to seasonally characterise the bacterial communities of HWW, including clinically relevant bacteria and resistance genes in Hospital Juárez de México (HJM), and to evaluate the impact of physicochemical factors on their composition. A one-year observational, cross-sectional study was conducted at five HWW discharge points of HJM. Fourteen physicochemical parameters were determined by using standard methodologies, and statistical differences between discharges and seasons were evaluated. Bacterial communities were analysed by targeted amplicon sequencing of the V3-V4 region of the 16S rRNA gene. In addition, the presence of eight antimicrobial resistance genes of local epidemiological importance was assessed. Data were analysed using alpha and beta diversity indices, principal component analysis, and multivariate statistical tests. HWW showed high taxonomic diversity, with Proteobacteria, Firmicutes, and Bacteroidetes standing out. Clinically relevant bacteria were identified in 73.3% of the analyses, with Enterobacter and Escherichia-Shigella predominating. Total and dissolved solids, temperature, nitrate, and pH significantly influenced the bacterial composition of HWW. Seven out of the eight genes evaluated were identified, with blaKPC, blaOXA-40, and mcr-1 being the most frequent, showing significant seasonal differences. This study underlines the microbiological and chemical complexity of HWW, highlighting the impact of clinically relevant bacteria and antimicrobial resistance genes on Public Health. The findings emphasise the need to implement hospital waste management programmes and ideally specific treatment plants to minimise the associated risks and protect the environment and human health. Full article
(This article belongs to the Section Environmental Microbiology)
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14 pages, 5563 KiB  
Article
Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae in Wastewater Effluent in Blantyre, Malawi
by Edna Ibrahim, Charity Mkwanda, Edward Masoambeta, Luigia Scudeller, Tomislav Kostyanev, Hussein H. Twabi, Yohane K. Diness, Jobiba Chinkhumba, Janelisa Musaya, Rajhab S. Mkakosya, Surbhi Malhotra-Kumar, Chantal M. Morel, Save Kumwenda and Chisomo L. Msefula
Antibiotics 2025, 14(6), 562; https://doi.org/10.3390/antibiotics14060562 - 30 May 2025
Viewed by 650
Abstract
Background/Objectives: Wastewater treatment plants (WWTPs) serve as a sink for both antimicrobial residues and bacteria carrying resistant genes, which are later disseminated into the environment, facilitating the spread of antimicrobial resistance. This study investigated the presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia [...] Read more.
Background/Objectives: Wastewater treatment plants (WWTPs) serve as a sink for both antimicrobial residues and bacteria carrying resistant genes, which are later disseminated into the environment, facilitating the spread of antimicrobial resistance. This study investigated the presence of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli (Ec), Klebsiella pneumoniae (Kp), and Enterobacter cloacae (Enc) in effluent from WWTP in Blantyre, Malawi, to generate evidence and provide baseline information for interventions. Methods: Selective chromogenic agar was used to identify ESBL-producing bacteria. Results: A total of 288 samples were collected between April 2023 and March 2024, and 97.6% (281/288) yielded one or more presumptive ESBL isolates. Bacterial growth was confirmed as 48.9% Ec (255/522), 33.0% Kp (172/522), and 10.0% Enc (52/522). Antibiotic susceptibility testing showed the highest resistance to ceftriaxone (Ec, 100.0%; Kp, 98.3%; Enc, 100.0%) and the lowest resistance to meropenem (Ec, 6.3%, Kp, 1.2%; Enc, 3.8%) among the antibiotics that were tested. Multiple antibiotic resistance phenotypes were observed in 73.1% of the isolates, with the most prevalent phenotype being amoxicillin + clavulanate/cotrimoxazole/doxycycline/ciprofloxacin/gentamicin/azithromycin/ceftriaxone (55, 15.7%). Conclusions: The study demonstrated ongoing environmental contamination with antibiotic-resistant bacteria from sewage effluent. Therefore, the functionality of WWTPs should be improved to minimize the release of these organisms into the environment. Full article
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