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17 pages, 566 KB  
Article
Analyst-of-Record: A Proof-of-Concept for Influence-Based Analyst Credit Assignment in Human-Feedback Decision Support
by Devon L. Brown and Danda B. Rawat
Electronics 2026, 15(6), 1210; https://doi.org/10.3390/electronics15061210 - 13 Mar 2026
Abstract
The purpose of this study is to examine whether analyst-level credit can be assigned quantitatively in a lightweight human-feedback decision-support pipeline. In intelligence and national security workflows, analysts often provide edits, comments, and evaluative feedback during the production of analytic products, yet these [...] Read more.
The purpose of this study is to examine whether analyst-level credit can be assigned quantitatively in a lightweight human-feedback decision-support pipeline. In intelligence and national security workflows, analysts often provide edits, comments, and evaluative feedback during the production of analytic products, yet these intermediate contributions are usually discarded, leaving no auditable record of how individual feedback shaped the final output. To address this problem, this study proposes a proof-of-concept Analyst-of-Record framework that combines synthetic analyst feedback, a linear ridge reward model, first-order influence functions, and additive Shapley aggregation to estimate both feedback-item and analyst-level contribution scores. The research design uses the Fact Extraction and VERification (FEVER) fact-verification dataset under controlled experimental settings. The pipeline retrieves evidence with Best Matching 25 (BM25), generates a grounded template-based response, derives three synthetic analyst feedback channels from FEVER annotations, trains a reward model on simple claim–answer and analyst-identity features, and aggregates per-feedback influence scores into an Analyst Contribution Index (ACI). The main experiments are conducted on a 500-claim subset across five random seeds, with additional ablation and bootstrap analyses used to assess sensitivity and stability. The findings show that the reward model achieves a mean validation R2 of 0.801±0.037, indicating that the synthetic feedback signals are learnable under the selected featureization. The analyst-level contribution scores remain stable across random seeds, with approximately half of the total influence magnitude attributed to the explanation-quality channel and the remainder split across the other two channels. Ablation results further show that removing the explanation-quality channel collapses validation fit, while bootstrap resampling demonstrates tight concentration of absolute ACI magnitudes. Theoretically, this study extends attribution research beyond document-only grounding by showing how analyst feedback itself can be modeled as an object of contribution analysis. It also demonstrates that influence functions and Shapley-style aggregation can be adapted into a tractable framework for estimating interpretable analyst-level credit in a reproducible experimental setting. Practically, the proposed framework offers an initial foundation for more traceable and accountable decision-support workflows in which intermediate analyst contributions can be preserved rather than lost. The results also provide a feasible implementation path for future systems that incorporate stronger generators, richer evidence representations, and real analyst annotations. Full article
(This article belongs to the Section Computer Science & Engineering)
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20 pages, 1841 KB  
Article
Seed Literacy and Access to Quality Seeds Among Smallholder Farmers in the Eastern Cape, South Africa: A Case Study of KwaMkhiva Village
by Walter Shiba, Mankaba Whitney Matli, Ntanda Gqutyana, Portia Mdwebi, Nomfundo Magagula, Siphe Zantsi and Michael Bairu
Sustainability 2026, 18(6), 2835; https://doi.org/10.3390/su18062835 - 13 Mar 2026
Abstract
Access to quality seed is a critical driver of smallholder productivity and household food security in South Africa, yet rural communities in the Eastern Cape continue to rely heavily on informal seed systems. Limited seed literacy among farmers and vendors is widely recognized [...] Read more.
Access to quality seed is a critical driver of smallholder productivity and household food security in South Africa, yet rural communities in the Eastern Cape continue to rely heavily on informal seed systems. Limited seed literacy among farmers and vendors is widely recognized as a constraint to the effective selection and use of high-quality seed. The purpose of this study is to assess seed literacy levels among smallholder farmers in KwaMkhiva village and evaluate how knowledge gaps shape farmers’ seed sourcing patterns and access to quality seed. The study hypothesizes that low seed literacy significantly increases reliance on informal seed systems and reduces adoption of certified or improved varieties. A quantitative, cross-sectional survey design was used to collect data from 50 smallholder farmers and 12 informal seedling vendors, complemented by semi-structured interviews with three extension officers. Descriptive statistics, chi-square tests, correlation analysis, and a composite Seed Literacy Index (SLI) were employed to assess literacy dimensions and their association with seed choices. Findings show that 49% of farmers rely on local markets and 40% use farm-saved seed, with 75% assessing quality visually rather than through germination or varietal indicators. Only 10% had received any seed-related training, and awareness of seed adaptability and crop rotation was below 20%. Higher SLI scores were positively associated with adoption of certified seed (r = 0.42, p < 0.01) and crop diversification. The study concludes that seed literacy is a critical yet underserved capability that shapes smallholder seed access within dual seed economies. Strengthening farmer-centred seed literacy programmes, revitalising extension services, and supporting community seed banks could enhance access to quality seed and improve smallholder resilience. Full article
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24 pages, 3361 KB  
Article
Simulation and Numerical Analysis of the Performance Parameters and Combustion Process of a Biofuel-Powered Compression Engine
by Paulina Mitan-Zalewska, Ewelina Kostecka, Irmina Durlik, Rafał Zalewski and Tymoteusz Miller
Energies 2026, 19(6), 1453; https://doi.org/10.3390/en19061453 - 13 Mar 2026
Abstract
This paper presents the analysis and results of the numerical simulation of the biofuel combustion process: namely, the volumetric mixture of diesel oil (ON) and camelina seed oil methyl ester (CSME) in a diesel engine. The mathematical model used in the simulation is [...] Read more.
This paper presents the analysis and results of the numerical simulation of the biofuel combustion process: namely, the volumetric mixture of diesel oil (ON) and camelina seed oil methyl ester (CSME) in a diesel engine. The mathematical model used in the simulation is based on a four-stroke diesel engine acting as a power generator. To enable simulations depending on the type of biofuel, a model algorithm was developed in the MATLAB/Simulink environment that allowed for the conditions and parameters to be adjusted according to specific test requirements. The numerical simulation was built on the basis of a real stand, in order to confirm the results of previous research both theoretically and in real applications. The calculation approach starts with the elemental composition of the fuel and goes through the intake, compression, combustion, and expansion stages, culminating in the thermal balance of the engine. The mathematical model confirmed the obtained results, which are comparable to the results from the research station. The obtained results confirm the legitimacy of using CSME as an additive to diesel and show its impact on engine performance that can be optimized to achieve the desired results. The use of pure CSME (100%) resulted in an increase in engine power and torque, probably due to the oxygen content of the biofuel molecules and its higher cetane number, which improves its ignition characteristics. However, an increase in unit fuel consumption has been observed, indicating lower energy efficiency compared to clean diesel, which is partially offset by the higher density of biofuel. The model takes into account the physicochemical properties of the fuel, such as the viscosity, cetane number and density, which significantly affect the fuel injection and atomization processes. Although the simulation is based on simplified assumptions, its results highlight the potential of biofuels in heavy transport and their cost-effectiveness as an alternative to fossil fuels. The developed model is used not only to evaluate the engine performance, but also as a tool for assessing the thermal efficiency, and optimizing the composition of the fuel mixture. Full article
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27 pages, 2643 KB  
Review
Common Buckwheat (Fagopyrum esculentum Mill.) as a Support for Sustainable Agriculture
by Piotr Jarosław Żarczyński, Ewa Mackiewicz-Walec, Sławomir Józef Krzebietke, Stanisław Sienkiewicz, Soňa Hlinková and Katarzyna Żarczyńska
Sustainability 2026, 18(6), 2823; https://doi.org/10.3390/su18062823 - 13 Mar 2026
Abstract
Common buckwheat (Fagopyrum esculentum Mill.) is a pseudocereal that has recently gained increasing interest among both farmers and scientists. Its low soil requirements, high adaptability, and high resistance to diseases and pests allow it to be cultivated in many regions of the [...] Read more.
Common buckwheat (Fagopyrum esculentum Mill.) is a pseudocereal that has recently gained increasing interest among both farmers and scientists. Its low soil requirements, high adaptability, and high resistance to diseases and pests allow it to be cultivated in many regions of the world. It is recommended for various cultivation systems, especially for low-input and organic farming. Currently, buckwheat is grown mainly for seeds and less often for green fodder. Thanks to its above-average nutritional value and many benefits that support human health, it is considered one of the leaders in functional food. It can be a basic raw material for many food products such as flour, groats, and flakes, but can also be used as a valuable addition to crisps, bars and drinks. Recently, buckwheat’s usefulness in the energy industry, construction, medicine, and pharmacology has been confirmed. Buckwheat, as a plant species distinct from the dominant global crops, fits very well into the current standards and assumptions of sustainable development. Its cultivation and consumption are associated with a number of benefits not only for human health but also for the whole environment. It is considered a species that counteracts climate change. Buckwheat’s valuable properties include its positive impact on soil physicochemical properties, its enhancement of biodiversity, and its support for pollinators. It is considered a species that can be cultivated in a changing climate, generating a very low carbon footprint. The aim of this study was to determine the contemporary economic importance of buckwheat, its place among species supporting sustainable development, and to identify potential research areas that will contribute to strengthening buckwheat’s role in sustainable agriculture. Full article
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29 pages, 10115 KB  
Article
ABA-Induced Transcriptomic Dynamics in Arabidopsis thaliana Anthers: Insights into Pollen Development and Fertility
by Lu Liu, Huiting Huang, Dexi Shi, Shuo Wang, Ziyi Lin, Fengming Huang, Li Huang and Sue Lin
Plants 2026, 15(6), 894; https://doi.org/10.3390/plants15060894 - 13 Mar 2026
Abstract
Pollen development is a complex process that is highly sensitive to environmental stresses. Abscisic acid (ABA), a key hormone mediating plant growth and stress responses, has been implicated in the regulation of sexual reproduction, especially pollen development, yet its precise regulatory role remains [...] Read more.
Pollen development is a complex process that is highly sensitive to environmental stresses. Abscisic acid (ABA), a key hormone mediating plant growth and stress responses, has been implicated in the regulation of sexual reproduction, especially pollen development, yet its precise regulatory role remains unclear. This study investigated the effects of exogenous ABA on Arabidopsis thaliana pollen development and function through integrated phenotypic, cytological, and transcriptomic approaches. ABA treatment specifically impaired pollen function by reducing germination rates and inhibiting pollen tube elongation, which resulted in shortened siliques and decreased seed set, without affecting pollen morphology or viability. Transcriptome analysis of mature anthers revealed a transient and time-dependent transcriptional response, with the number of differentially expressed genes (DEGs) peaking at 8 h post-ABA treatment and markedly declining by 22 h. These DEGs were enriched in stress-response pathways (e.g., salt, cold, and dehydration), hormone signaling, and carbohydrate metabolism. Moreover, we identified 25 differentially expressed transcription factors and 16 pollen development and function-related genes, highlighting their key roles in ABA-mediated regulation. In parallel, 146 differentially expressed lncRNAs (DELs) were identified, which formed 144 cis-regulatory pairs with genes involved in ABA response and pollen tube growth, with their predicted targets enriched in pathways such as hormone and MAPK signaling, carbohydrate metabolism and stress response. Trans-regulatory analysis further revealed that these DELs co-expressed with DEGs in modules enriched for stress response, pollen development, and tube growth pathways. Notably, key pollen function genes showed strong co-expression with DELs, indicating that lncRNAs participate in ABA-induced transcriptional reprogramming that shifts metabolic resources from growth to defense, thereby suppressing pollen germination and tube elongation. Together, these findings elucidate a coordinated regulatory network involving mRNAs, lncRNAs and transcription factors roles in modulating ABA responses during pollen/anther development. Full article
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29 pages, 1808 KB  
Review
Strawberry Propagation: Progress on Propagation Methods, Environmental Regulation, and Disease Management Strategies over the Past 20 Years
by YoungHun Lee, HyunSik Yeoung, Bruno Mezzetti and YoungRog Yeoung
Horticulturae 2026, 12(3), 351; https://doi.org/10.3390/horticulturae12030351 - 13 Mar 2026
Abstract
Strawberry (Fragaria × ananassa Duch.) propagation has evolved significantly over the past 20 years, transitioning from traditional field nursery systems to advanced, controlled, environment production. This review synthesizes recent advances in propagation methods, environmental regulation, and disease management strategies. Traditional field systems [...] Read more.
Strawberry (Fragaria × ananassa Duch.) propagation has evolved significantly over the past 20 years, transitioning from traditional field nursery systems to advanced, controlled, environment production. This review synthesizes recent advances in propagation methods, environmental regulation, and disease management strategies. Traditional field systems face mounting challenges from soilborne pathogens (Neopestalotiopsis species, Phytophthora cactorum, Verticillium dahliae) and regulatory restrictions on methyl bromide fumigation. Plug plant technology offers 80–95% disease reduction and 3–7-week production cycles versus 12–16-weeks traditional cycles, although at higher unit costs. Advanced tray plant systems developed in the Netherlands enable 10–11 months cold storage and programmed year-round production schedules. Elevated bench propagation systems have emerged as dominant commercial technology in East Asian regions, particularly Korea and Japan, where disease pressure necessitated alternatives to conventional nurseries. Micropropagation via temporary immersion bioreactors achieves 50–100% higher multiplication rates, while ensuring virus-free status. Environmental control research reveals complex photoperiod–temperature-chilling interactions regulating dormancy and flowering. Emerging technologies include F1 hybrid seed propagation and AI-driven automation, achieving 15–25% energy efficiency gains. Despite progress, challenges remain in cost optimization, climate adaptation, and region-specific protocols. This review provides a comparative framework for nursery system selection under evolving climatic and regulatory constraints, identifying critical knowledge gaps and future research priorities for sustainable strawberry propagation. Full article
(This article belongs to the Section Propagation and Seeds)
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20 pages, 2008 KB  
Article
Development of an Efficient Somatic Embryogenesis Protocol for Carica papaya L. Var. TNAU Papaya CO 8 on Different Basal Media
by Shalini Chandrasekar, Kavitha Chinnasamy, Ganga Mathian, Krish K Kumar, Babu Rajendra Prasad, Manoranjitham S. Karuppannan, Selvaraju Kanagarajan and Saraladevi Muthusamy
Plants 2026, 15(6), 893; https://doi.org/10.3390/plants15060893 - 13 Mar 2026
Abstract
Papaya (Carica papaya L.) is a highly cross-pollinated crop that exhibits considerable genetic variability when propagated through seeds, resulting in non-true-to-type progeny. Therefore, the development of an efficient in vitro regeneration system is essential for large-scale clonal propagation of elite cultivars. In [...] Read more.
Papaya (Carica papaya L.) is a highly cross-pollinated crop that exhibits considerable genetic variability when propagated through seeds, resulting in non-true-to-type progeny. Therefore, the development of an efficient in vitro regeneration system is essential for large-scale clonal propagation of elite cultivars. In the present study, a highly efficient and reproducible somatic embryogenesis protocol was developed for C. papaya var. TNAU Papaya CO 8 using immature zygotic embryos as explants. This study provides the first comprehensive comparative evaluation of three basal media, viz., Murashige and Skoog Medium, N6 Medium, and Woody Plant Medium, for somatic embryogenesis and plant regeneration in this variety, along with the optimization of polyamine-enriched media for enhanced plantlet recovery. The embryogenic potential of explants was assessed across different stages, including callus induction, somatic embryo development, plant regeneration, shoot elongation, rooting, and acclimatization. Maximum callus induction (81.96%) was observed on half-strength MS medium supplemented with 2,4-Dichlorophenoxyacetic acid under dark conditions, followed by ½ N6 (63.00%) and ½ WPM (58.02%). Somatic embryo initiation was highest on ½ MS medium containing 2.0 mgL−1 2,4-D (77.82%). Somatic embryos developed through distinct globular, heart, torpedo, and cotyledonary stages. Embryo maturation was significantly enhanced on MS medium supplemented with abscisic acid, polyethylene glycol, benzylaminopurine, and proline. The highest plantlet regeneration (85.02%) was achieved on MS medium enriched with putrescine, whereas comparatively lower regeneration was recorded on N6 (75.99%) and WPM (57.97%). Shoot elongation was significantly improved by supplementation with gibberellic acid (1.0 mgL−1). Root induction was optimal on half-strength MS medium containing Indole-3-butyric acid, 1-Naphthaleneacetic acid, phloroglucinol, and activated charcoal, resulting in well-developed roots. Regenerated plantlets were successfully acclimatized in a cocopeat–vermicompost substrate with a survival rate of 74.01%. The optimized protocol provides a reliable and efficient system for large-scale clonal propagation and offers promising applications in genetic transformation and commercial production of papaya var. TNAU papaya CO 8. Full article
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23 pages, 3713 KB  
Article
Plant Growth Promoting Rhizobacteria Favor Vegetative Development and Optimize Nutrient Uptake in Lisianthus
by Tsujmejy Gómez-Navor, Fernando Carlos Gómez-Merino, Juan José Almaraz-Suárez, Marco Polo Carballo-Sánchez, J. Cruz García-Albarado and Libia Iris Trejo-Téllez
Horticulturae 2026, 12(3), 350; https://doi.org/10.3390/horticulturae12030350 - 13 Mar 2026
Abstract
Lisianthus [Eustoma grandiflorum (Raf.) Shinners] is among the 10 most produced and marketed cut flowers in the world. However, its slow growth represents a challenge for its production. This study evaluated the efficiency of rhizobacterial strains in vegetative growth and nutrient acquisition [...] Read more.
Lisianthus [Eustoma grandiflorum (Raf.) Shinners] is among the 10 most produced and marketed cut flowers in the world. However, its slow growth represents a challenge for its production. This study evaluated the efficiency of rhizobacterial strains in vegetative growth and nutrient acquisition in lisianthus plants. Freshly germinated seeds of lisianthus cv. Mariachi Blue Double were used. Seven rhizobacterial strains and two controls (sterile distilled water and nutrient broth) were evaluated in a completely randomized design. Replication varied among treatments and variables: shoot growth traits were assessed on 10–12 plants per treatment, root biomass on a destructive subsample of six plants per treatment and shoot nutrient contents on four composite samples per treatment. Measurements taken 149 days after sowing showed that plants inoculated with the strains Acinetobacter vivianii C48, Achromobacter xylosoxidans C56, and Arthrobacter pokkalii JLB4 had greater height, leaf area, leaf number, and fresh and dry biomass, both aerial and in the root. These strains also enhanced N and P uptake in shoot tissues. In contrast, the Bacillus pumilus strain R44 significantly decreased height and leaf number. The results suggest that strains C48, C56 and JLB4 can stimulate nutrition, accelerate plant growth, and shorten the vegetative phase in lisianthus. Full article
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19 pages, 3185 KB  
Article
Sown Summer-Blooming Wildflowers as a Tool to Support Pollinator Biodiversity During Dry Periods in Mediterranean Agroecosystems
by Stefano Benvenuti
Plants 2026, 15(6), 887; https://doi.org/10.3390/plants15060887 - 12 Mar 2026
Abstract
Summer abiotic stresses typical of Mediterranean agro-environments, now exacerbated by climate change, reduce floral resource availability and further compromise the survival of pollinators already threatened in the so-called Anthropocene. The aim of this study was to evaluate several summer-blooming wildflower species, collected from [...] Read more.
Summer abiotic stresses typical of Mediterranean agro-environments, now exacerbated by climate change, reduce floral resource availability and further compromise the survival of pollinators already threatened in the so-called Anthropocene. The aim of this study was to evaluate several summer-blooming wildflower species, collected from ecologically disturbed and diversified habitats, in order to assess their ecological role in attracting pollinators within agroecosystems. The primary dormancy typical of wild species seeds was partially overcome through appropriate pre-sowing seed treatments, while secondary dormancy was reduced by soil rolling after sowing. Soil rolling proved particularly beneficial for species with very small seeds, highlighting the importance of adequate seed–soil contact for successful establishment. All tested species exhibited summer flowering between May and July, with some flowering later in the season, and showed high attractiveness to pollinators both in terms of abundance and taxonomic diversity. However, this ecosystem service declined significantly in the second year, although certain species demonstrated a strong capacity to persist and to maintain satisfactory pollinator attractiveness over time. In conclusion, while the experiment revealed several critical aspects, it also provides encouraging prospects for further research aimed at enhancing pollinator survival in agroecosystems that are severely threatened by pollinator decline. Full article
(This article belongs to the Special Issue Interaction Between Flowers and Pollinators)
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21 pages, 17701 KB  
Article
Synergistic Effects of Ammonia and Hypoxia Stress on the Transcriptomic Responses of the Razor Clam (Sinonovacula constricta)
by Zidai Liu, Hao Zhang, Congying Lai, Ran Sun, Hongqiang Xu, Hanhan Yao, Yinghui Dong, Zhihua Lin and Liyuan Lv
Animals 2026, 16(6), 896; https://doi.org/10.3390/ani16060896 - 12 Mar 2026
Abstract
The co-occurrence of ammonia nitrogen and hypoxia represents a physiologically taxing synergistic challenge for benthic bivalves—as it forces a conflict between the high energy demand for detoxification and the limited energy supply under low oxygen, yet the tissue-specific strategies underlying their resilience remain [...] Read more.
The co-occurrence of ammonia nitrogen and hypoxia represents a physiologically taxing synergistic challenge for benthic bivalves—as it forces a conflict between the high energy demand for detoxification and the limited energy supply under low oxygen, yet the tissue-specific strategies underlying their resilience remain poorly understood. This study investigated the physiological and transcriptomic responses of the razor clam Sinonovacula constricta to ammonia (AG), hypoxia (HG), and their combination (HAG) over 96 h. Transcriptomic profiling revealed that the gill and hepatopancreas employ distinct, organ-coordinated adaptive strategies rather than a uniform systemic response. The gill prioritized respiratory homeostasis by fine-tuning oxygen sensing: transcriptional suppression of hypoxia-inducible factor 1-α (HIF-1α) (to limit glycolytic acidosis) was followed by a chronic induction of HIF-2α, alongside the specific upregulation of the mitochondrial respiratory gene cytochrome c oxidase-6b (COX-6b). In contrast, the hepatopancreas executed a critical metabolic trade-off centered on arginine metabolism. Under combined stress, arginine flux was redirected toward the urea cycle via a robust upregulation of arginase (ARG) for detoxification, while nitric oxide synthase (NOS) was concurrently suppressed. This reciprocal regulation suggests a strategy to prioritize ammonia clearance and energy conservation at the expense of immune signaling. These findings elucidate how S. constricta navigates the bioenergetic conflict between detoxification and oxygen limitation, providing molecular targets for breeding stress-resistant aquaculture strains. Full article
(This article belongs to the Special Issue Environmental Adaptation and Metabolic Regulation in Aquatic Animals)
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30 pages, 1238 KB  
Article
Activation-Guided Layer Selection for LoRA
by Aditya Dawadikar, Pooja Shyamsundar, Rashmi Vishwanath Bhat and Navrati Saxena
Information 2026, 17(3), 283; https://doi.org/10.3390/info17030283 - 12 Mar 2026
Abstract
Low-Rank Adaptation (LoRA) has become a widely adopted parameter-efficient fine-tuning (PEFT) technique for large language models (LLMs). LoRA’s benefits stem from its light weight and modular adapters. Standard LoRA applies adapters uniformly across all Transformer layers, implicitly assuming that each layer contributes equally [...] Read more.
Low-Rank Adaptation (LoRA) has become a widely adopted parameter-efficient fine-tuning (PEFT) technique for large language models (LLMs). LoRA’s benefits stem from its light weight and modular adapters. Standard LoRA applies adapters uniformly across all Transformer layers, implicitly assuming that each layer contributes equally to task adaptation. However, LLMs are found to have internal substructures that contribute in a disproportionate manner. In this work, we provide a theoretical analysis of how LoRA weight updates are influenced by a layer’s activation magnitude. We propose Act-LoRA, a simple activation-guided layer selection strategy for selective Low-Rank Adaptation. We evaluate this strategy for both encoder-only and decoder-only architectures using the GLUE benchmark. Our method achieved a 20% GPUh saving with a 1% drop in GLUE score using DeBERTaV3-Base on a single-instance GPU with 50% less LoRA parameters. It also achieved 2% GPUh savings with a less than 0.15% drop in GLUE score with the Llama-3.1-8B model in Distributed Data Parallel mode with 25% fewer LoRA parameters. Our experiments and analysis show that the compute and memory requirements of LoRA adapters increase linearly with the number of selected layers. We further compare activation-guided selection against gradient-guided importance metrics and show that activation norms yield more stable and reproducible layer rankings across seeds and datasets. Overall, our results demonstrate that activation-guided layer selection is a practical and effective way to improve the efficiency of LoRA fine-tuning, making it immediately compatible with some existing PEFT techniques and distributed training frameworks. Full article
(This article belongs to the Special Issue Modeling in the Era of Generative AI)
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19 pages, 17864 KB  
Article
The Enhancement of Abiotic Stress Tolerance in Arabidopsis via Heterologous Overexpression of TcDHN1, a Dehydrin Identified in the Recalcitrant Seeds of Taxillus chinensis
by Ya Qin, Yuqiong Li, Cuihong Yang, Wenjing Liang, Lingjian Gui, Lisha Song, Jie Shen, Ru Chen, Limei Pan, Shugen Wei and Lingyun Wan
Plants 2026, 15(6), 884; https://doi.org/10.3390/plants15060884 - 12 Mar 2026
Abstract
Taxillus chinensis (DC.) Danser is an important hemiparasitic medicinal plant whose propagation is severely limited by the desiccation sensitivity of its recalcitrant seeds. Dehydrins (DHNs), which protect plants against dehydration-induced stresses such as salinity, drought, and low temperatures, may play a critical role [...] Read more.
Taxillus chinensis (DC.) Danser is an important hemiparasitic medicinal plant whose propagation is severely limited by the desiccation sensitivity of its recalcitrant seeds. Dehydrins (DHNs), which protect plants against dehydration-induced stresses such as salinity, drought, and low temperatures, may play a critical role in protecting recalcitrant seeds. However, the role of DHNs in the seeds of T. chinensis remains unclear. In this study, a differentially expressed gene was identified from the seed transcriptome of T. chinensis and designated TcDHN1. Sequence alignment and phylogenetic analyses revealed that TcDHN1 encodes a dehydrin protein. Heterologous overexpression of TcDHN1 in Arabidopsis did not affect growth under normal conditions. Under salt, drought, and cold stresses, transgenic lines exhibited higher seed germination rates, longer primary roots, and improved seedling growth compared with wild-type (WT) plants. The transgenic lines showed significantly increased activities of antioxidant enzymes, including superoxide dismutase, catalase, and peroxidase. In addition, ectopic overexpression of TcDHN1 in Arabidopsis conferred enhanced tolerance to abiotic stresses compared to WT plants, accompanied by increased expression of the stress-responsive genes Responsive to Desiccation 29A (AtRD29A) and Heat Shock Protein 70-1 (AtHSP70-1). The above results indicate that TcDHN1 confers enhanced tolerance to abiotic stresses. This study provides a functional characterization of an abiotic stress-responsive gene from recalcitrant seeds and identifies a potential genetic resource for molecular breeding. This could potentially improve abiotic stress resistance in T. chinensis and related medicinal plants. Full article
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19 pages, 1469 KB  
Article
Spatial Variations in Seed Germination Traits of White Spruce (Picea glauca) and Black Spruce (P. mariana) Across the Canadian Boreal Forest
by Elaine Qualtiere, Yongsheng Wei, Dustin Snider, Yuguang Bai, Mark Johnston, Daniel W. McKenney, Pia Papadopol and Dale Simpson
Plants 2026, 15(6), 882; https://doi.org/10.3390/plants15060882 - 12 Mar 2026
Abstract
This study focuses on the spatial variation in seed germination characteristics of Picea glauca and P. mariana, prominent and widespread species within the Canadian boreal forest. The main objective was to determine seed germination requirements of geographically distinct seed collections of P. [...] Read more.
This study focuses on the spatial variation in seed germination characteristics of Picea glauca and P. mariana, prominent and widespread species within the Canadian boreal forest. The main objective was to determine seed germination requirements of geographically distinct seed collections of P. glauca and P. mariana. A total of 73 collections of P. glauca and 62 collections of P. mariana were selected across Canada and tested for germination under various temperatures. Base temperature (Tb) and thermal time required to reach 50% germination (TH50) were derived from thermal model parameters for all seed collections. Correlation analyses between seed germination traits, geographic, and climatic variables were conducted. Base temperatures for germination of P. glauca ranged from 5.2 to 11.9 °C while P. mariana had base temperatures ranging from 6.2 to 12.8 °C, indicating a broader temperature range for the former to initiate germination. Optimal germination temperatures ranged from 15 to 20 °C for P. glauca and from 17.5 to 30 °C for P. mariana. Thermal time requirements for 50% germination were higher for P. glauca than for P. mariana, indicating that the former takes longer to germinate under the same temperature conditions. Latitudinal-related variables such as temperature of sites had a stronger influence on germination relative to precipitation or potential evaporation and affected seed viability, final germination and germination capacity of all seed sources. Seed viability was lower in northern seed collections and germination capacity was diminished at lower temperatures for both species. The results from this study can be built into models predicting shifts in boreal forest species under climate change. Full article
(This article belongs to the Special Issue Seed Dormancy and Germination for Plant Adaptation to Climate Change)
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36 pages, 417 KB  
Article
A Dynamical Approach to General Relativity Based on Proper Time
by Jaume de Haro
Universe 2026, 12(3), 79; https://doi.org/10.3390/universe12030079 - 12 Mar 2026
Abstract
This work places the invariant ds2 at the center of the gravitational interaction, interpreting it not as a purely geometric object but as the differential of proper time, endowed with direct physical meaning. Starting from the extension of Fermat’s principle to [...] Read more.
This work places the invariant ds2 at the center of the gravitational interaction, interpreting it not as a purely geometric object but as the differential of proper time, endowed with direct physical meaning. Starting from the extension of Fermat’s principle to massive particles—namely, the requirement that freely falling bodies follow trajectories that extremize proper time, which for timelike motion corresponds to a local maximum—and invoking the universality of Galilean free fall, we derive the form of ds2 in a static gravitational field. Lorentz invariance then provides the natural framework to extend this result to systems involving moving matter. The invariant derived through this procedure matches the weak-field limit of General Relativity formulated in the harmonic gauge. Within this linearized regime, we show that the structure of the theory already contains the seeds of its nonlinear completion: any dynamically consistent extension to strong gravitational fields necessarily involves the Ricci tensor. From this viewpoint, Einstein’s field equations appear not as a postulated geometric law but as the unique covariant closure required to ensure energy–momentum conservation and the self-consistency of the gravitational interaction. Full article
15 pages, 2848 KB  
Article
Is Plasma Treatment of Commodity Lettuce Seeds Worth It? Economic Impacts and Yield Study in Indoor Vertical Farming Testing Non-Thermal Plasmas
by Nima Asgari, Nan Zou, Ying Zheng and Joshua M. Pearce
Commodities 2026, 5(1), 6; https://doi.org/10.3390/commodities5010006 - 12 Mar 2026
Abstract
Agricultural seeds are sold as commodities yet seed quality can be non-uniform. Despite the extensive literature showing that plasma treatments of seeds provides advantages for many crops, lettuce studies, particularly in indoor farming systems, are limited. This study provides a systematic investigation of [...] Read more.
Agricultural seeds are sold as commodities yet seed quality can be non-uniform. Despite the extensive literature showing that plasma treatments of seeds provides advantages for many crops, lettuce studies, particularly in indoor farming systems, are limited. This study provides a systematic investigation of the impacts of non-thermal plasma treatments with various feed gases (N2, O2, dry air, and wet air) on the germination and growth characteristics of four lettuce cultivars (Red Oakleaf (RO), Black Simpson (BS), Valley Heart Romaine (VHR), and Paris Romaine (PR)) under controlled cultivation conditions in an agrivoltaic agrotunnel. Although the germination time was not conclusively affected by the treatments, the results show a complex interaction between germination rate and yield across the different cultivars and plasma treatments. Except for PR seeds (77.8% vs. 65.8% control), wet air plasma treatments increased germination rates by 18.7–100% over controls for all other cultivars. In yield analysis, wet air treatment had the strongest effect, especially for VHR (51.7 vs. 42.5 g/pot). Treatments did not notably affect RO. For BS, N2 treatment gave the highest increase (54.2 vs. 48.1 g/pot), while PR responded best to O2 treatment (58.4 vs. 51.8 g/pot). The energy consumption of plasma treatments was negligible for all treatments, while labor costs for small batches of seeds accounted for the largest share of secondary operating costs (839, 622, and 659 h/year, respectively for BS, VHR, and PR). Despite additional expenses, including labor, O&M, and degradation costs, the reduced seed requirements from higher germination rates and higher yield increased net profit by 12.0% compared to untreated cultivation in the most impacted (Valley Heart Romaine) lettuce. There is an opportunity for further cost optimization of the non-thermal plasma treatment for each type of lettuce seed. Full article
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