Search Results (98)

Foliar application of biostimulants can be a valid option to reach the goal of sustainable intensification in agriculture, especially in extensive crops such as durum wheat. However, due to the wide range of active ingredients and their mixtures available in the market, the need to select the most efficient product in a specific growing environment is of dramatic importance to achieve remarkable results in yield and grain quality. To analyze the potential of different active ingredients, a field trial was performed in two consecutive growing seasons (2023 and 2024) under Mediterranean climatic conditions. A randomized block design with three replicates was used. Durum wheat cultivar “Iride” was treated with the following five foliar biostimulants in comparison with the untreated control (T0): seaweed and plant extracts (T1); micronized vaterite (T2); culture broth of Pseudomonas protegens (T3); humic and fulvic acids (T4); organic nitrogen fertilizer (N 5%) containing glycine betaine (T5). Biostimulant treatment was applied at the end of tillering and at heading. Root length, chlorophyll content, grain yield, yield components and grain quality were measured and subjected to a one-way analysis of variance. As compared to the control, seaweed and plant extracts as well as micronized vaterite showed the best results in terms of grain yield (29% and 24% increase, respectively), root length (120% and 77% increase, respectively) and grain protein content (one percentage point increase, from approx. 12% to 13%). The results from this study can help Mediterranean farmers and researchers to develop new fertilization protocols to reach the goals of the “Farm to Fork” European strategy. Full article

Livestock farming is a vital component of global food security, yet it remains a major contributor to greenhouse gas (GHG) emissions, particularly methane (CH₄), which has a global warming potential 28 times greater than carbon dioxide (CO₂). This review provides a comprehensive synthesis of current knowledge surrounding the sources, biological mechanisms, and mitigation strategies related to CH₄ emissions from ruminant livestock. We first explore the process of methanogenesis within the rumen, detailing the role of methanogenic archaea and the environmental factors influencing CH₄ production. A thorough assessment of both direct and indirect methods used to quantify CH₄ emissions is presented, including in vitro techniques (e.g., syringe method, batch culture, RUSITEC), in vivo techniques (e.g., respiration chambers, Greenfeed, laser CH₄ detectors), and statistical modeling approaches. The advantages and limitations of each method are critically analyzed in terms of accuracy, cost, feasibility, and applicability to different farming systems. We then examine a wide range of mitigation strategies, organized into four core pillars: (1) animal and feed management (e.g., genetic selection, pasture quality improvement), (2) diet formulation (e.g., feed additives such as oils, tannins, saponins, and seaweed), (3) rumen manipulation (e.g., probiotics, ionophores, defaunation, vaccination), and (4) manure management practices and policy-level interventions. These strategies are evaluated not only for their environmental impact but also for their economic and practical viability in diverse livestock systems. By integrating technological innovations with sustainable agricultural practices, this review highlights pathways to reduce CH₄ emissions while maintaining animal productivity. It aims to support decision-makers, researchers, and livestock producers in the global effort to transition toward climate-smart, low-emission livestock farming. Full article

The need to maximize aquaculture production while addressing environmental and food security challenges posed by climate change has driven research towards the development of functional aquafeeds that enhance performance and immunity in farmed species. However, exposure to dietary and environmental stressors affects marine organisms, altering key metabolic pathways best understood through high-throughput “omics” tools. This study assessed the effects of Asparagopsis taxiformis supplementation on central metabolic pathways by analyzing changes in primary metabolite levels in the liver of farmed Diplodus sargus under optimal and suboptimal temperature conditions. Results showed that seaweed supplementation had a beneficial effect on the fish’s primary metabolome; however, inclusion levels and rearing conditions played a crucial role in determining outcomes. While 1.5% supplementation maintained a balanced primary metabolome under optimal temperature conditions, 3.0% supplementation most effectively mitigated the adverse effects of acute thermal stress during a marine heatwave. These findings highlight the nutritive and functional potential of A. taxiformis supplementation in aquafeeds for marine omnivorous fish species and emphasize the importance of evaluating functional aquafeeds under suboptimal rearing conditions. Overall, our results demonstrate the value of metabolomics in elucidating the molecular basis underlying biological pathways in farmed marine fish and optimizing production through climate-smart dietary strategies. Full article

In recent years, global agriculture has encountered several challenges exacerbated by the effects of changes in climate, such as extreme water shortages for irrigation and heat waves. Water-deficit stress adversely affects the morpho-physiology of numerous crops, including soybean (Glycine max L.), which is considered as promising crop in Bangladesh. Seaweed extract (SWE) has the potential to improve crop yield and alleviate the adverse effects of water-deficit stress. Remote and proximal sensing are also extensively utilized in estimating morpho-physiological traits owing to their cost-efficiency and non-destructive characteristics. The study was carried out to evaluate soybean morpho-physiological traits under the application of water extracts of Gracilaria tenuistipitata var. liui (red seaweed) with two varying irrigation water conditions (100% of total crop water requirement (TCWR) and 70% of TCWR). Principal component analysis (PCA) revealed that among the four treatments, the 70% irrigation + 5% (v/v) SWE and the 100% irrigation treatments overlapped, indicating that the application of SWE effectively mitigated water-deficit stress in soybeans. This result demonstrates that the foliar application of 5% SWE enabled soybeans to achieve morpho-physiological performance comparable to that of fully irrigated plants while reducing irrigation water use by 30%. Based on Pearson’s correlation matrix, a simple linear regression model was used to ascertain the relationship between unmanned aerial vehicle (UAV)-derived vegetation indices and the field-measured physiological characteristics of soybean. The Normalized Difference Red Edge (NDRE) strongly correlated with stomatal conductance (R² = 0.76), photosystem II efficiency (R² = 0.78), maximum fluorescence (R² = 0.64), and apparent transpiration rate (R² = 0.69). The Soil Adjusted Vegetation Index (SAVI) had the highest correlation with leaf relative water content (R² = 0.87), the Blue Normalized Difference Vegetation Index (bNDVI) with steady-state fluorescence (R² = 0.56) and vapor pressure deficit (R² = 0.74), and the Green Normalized Difference Vegetation Index (gNDVI) with chlorophyll content (R² = 0.73). Our results demonstrate how UAV and physiological data can be integrated to improve precision soybean farming and support sustainable soybean production under water-deficit stress. Full article

Helicobacter pylori is a gastric pathogen implicated in peptic ulcer disease and gastric cancer. The increasing prevalence of antibiotic-resistant strains underscores the urgent need for alternative therapeutic strategies. In this study, we investigated the chemical composition and antibacterial activity of an aqueous extract from Caulerpa lentillifera (sea grape), a farm-cultivated edible green seaweed collected from Krabi Province, Thailand. Ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) revealed that the extract was enriched in bioactive nucleosides and phenolic compounds. In vitro assays demonstrated dose-dependent inhibition of H. pylori growth following exposure to sea grape extract. Furthermore, untargeted intracellular metabolomic profiling of H. pylori cells treated with the extract uncovered significant perturbations in central carbon and nitrogen metabolism, including pathways associated with the tricarboxylic acid (TCA) cycle, one-carbon metabolism, and alanine, aspartate, and glutamate metabolism. Pyrimidine biosynthesis was selectively upregulated, indicating a potential stress-induced shift toward nucleotide salvage and DNA repair. Of particular note, succinate levels were markedly reduced despite accumulation of other TCA intermediates, suggesting disruption of electron transport-linked respiration. These findings suggest that bioactive metabolites from C. lentillifera impair essential metabolic processes in H. pylori, highlighting its potential as a natural source of antimicrobial agents targeting bacterial physiology. Full article

Marine heatwaves (MHWs) are an emerging challenge for aquaculture, increasing the frequency and severity of disease outbreaks in farmed fish, weakening immunocompetence and compromising overall health and survival. As climate change stressors intensify, there is an urgent need for cost-effective and environmentally friendly strategies to enhance fish resilience. This study investigated the efficacy of Laminaria digitata, a brown macroalga, included in aquafeeds as powder (0.3% and 1.5%) or extract (0.3%) in improving the stress response of juvenile gilthead seabream (Sparus aurata) co-exposed to Vibrio harveyi infection during a category III MHW event. Under control conditions, L. digitata supplementation maintained or improved fish growth performance and overall well-being. After MHW exposure, the 1.5% powdered and 0.3% extract diets were more effective in mitigating thermal stress, reducing liver oxidative stress and lowering plasma cortisol levels. In infected fish, these diets improved resistance to V. harveyi, with reduced cortisol and alanine aminotransferase concentrations indicating hepatoprotective properties, and lower lipid peroxidation and decreased antioxidant enzyme activities reflecting an amplified capacity to counteract oxidative stress induced by inflammation. Overall, L. digitata is a promising aquafeed supplement, with the 1.5% powdered form offering a cost-effective alternative to the extract without compromising efficacy. Full article

The aquaculture sector is essential for meeting seafood demand while ensuring sustainability. It involves farming fish, mollusks, crustaceans, other invertebrates, and algae in controlled environments, helping to conserve marine resources and reduce ecological pressures. Sustainable practices, such as an integrated multitrophic recirculating aquaculture system (IMTA-RAS) with fish and seaweed, can minimize the environmental impact of fish aquaculture. However, the impact of the introduction of macroalgae on the fish muscle metabolism has not been studied. This research examines the impact of growing Senegalese sole (Solea senegalensis) together with sea lettuce (Ulva ohnoi) on fish metabolism using high-resolution ¹H-NMR-based metabolomics. Three farming systems were compared. These were E₁, a recirculating aquaculture system (RAS); E₂, an IMTA-RAS integrating U. ohnoi for biofiltration; and E₃, an IMTA-RAS with U. ohnoi and Phaeobacter sp. strain 4UAC3, a probiotic bacterium isolated from wild U. australis known to counteract fish pathogens. A metabolomic analysis revealed that energy metabolism was enhanced in IMTA-RAS and even more in IMTA-RAS-Phaeobacter–grown fish, increasing overall metabolic activity. These results indicate that the presence of the algae with the probiotic had a clear impact on the physiological state of the fish, and this deserves further investigation. This study contributes to the understanding of the physiological responses of fish to innovative aquaculture practices, supporting the development of more sustainable and efficient management that reduces the environmental impact and increases fish health and welfare. Full article

Worker safety has been relatively overlooked in the rapidly growing aquaculture industry. To address this gap, industrial accident compensation insurance data—mainly from floating cage and seaweed farming—were analyzed to quantify accident types and frequencies, with a focus on human elements as root causes. Basic causes were selected based on IMO Resolution A/Res.884 and assessed through a worker awareness survey. Fault Tree Analysis (FTA), a Formal Safety Assessment technique, was applied to evaluate risks associated with these causes. The analysis identified organization at the farm site (23.3%), facility and equipment factors (22.8%), and people factors (21.4%) as the primary causes. Among secondary causes, personal negligence (13.2%), aging gear and poor maintenance (11.4%), and insufficient risk training (10.4%) were the most significant. Selective removal of these causes reduced the probability of human element-related accidents from 64.6% to 48.6%. While limited in scope to Korean data and self-reported surveys, the study demonstrates the value of combining quantitative data with worker perspectives. It provides foundational data for developing tailored safety strategies and institutional improvements—such as standardized procedures, multilingual education, and inclusive risk management—for sustainable safety in aquaculture. Full article

Soybean cultivation plays a crucial role in the global food system, providing raw materials for both the food and feed industries. To enhance cultivation efficiency, plant biostimulants are used to improve metabolism and stimulate growth. A key aspect of modern cultivation is the ability to rapidly and non-invasively assess crop status. One such method involves the use of drones equipped with multispectral cameras. This paper presents the results of an experimental study on soybean cultivation involving a natural biostimulant in the form of Epilobium angustifolium extract (commonly known as fireweed) and a commercial seaweed-based biostimulant, Kelpak. The research was conducted at an experimental farm in eastern Poland. The effectiveness of the preparations was evaluated using a drone-mounted multispectral camera. Changes in the values of selected spectral indices were analyzed: the Normalized Difference Red Edge Index (NDRE), the Leaf Chlorophyll Index (LCI), and the Optimized Soil-Adjusted Vegetation Index (OSAVI). The study included a control group treated with pure water. Mathematical and statistical analyses of the mean values and standard deviations of the indices were conducted. The results demonstrated that multispectral scanning allows for the detection of significant differences between the effects of the E. angustifolium extract, the seaweed-based biostimulant, and the water control. These findings confirm the utility of this method for assessing the effectiveness of biostimulant applications in soybean cultivation. Full article

Seaweeds and their derived products have long been valued in organic agriculture, serving roles in biofertilizers, biostimulants, and soil conditioners due to their rich content of bioactive compounds. With increasing concerns over the negative impacts of synthetic agrochemicals on food security and environmental health, seaweeds offer a sustainable alternative for improving soil fertility and crop productivity. This review synthesizes recent findings on the use of seaweeds to enhance soil physicochemical properties, stimulate beneficial microbial activity, and improve nutrient availability. Furthermore, it highlights how seaweed applications can mitigate various abiotic stresses, such as droughts, salinity, and nutrient deficiency, by enhancing antioxidant defenses and promoting physiological and biochemical resilience in plants. Key agronomic benefits include improved seed germination, root development, photosynthesis, biomass accumulation, and yield performance. By acting as natural soil amendments, seaweeds support sustainable soil management and contribute to long-term agricultural resilience. This review emphasizes the urgent need for standardized application strategies and integrated research to unlock the full potential of seaweed-based solutions in sustainable farming systems. Full article

Marine environments provide a unique opportunity to blend offshore wind energy production and marine fishery activities as complementary technologies. This study investigated the morphological characteristics (length and weight) and biomass yield of seaweed (Undaria pinnatifida) in a model marine environment with mariculture within an offshore wind farm in southwestern Korea. The mean lengths in the first cultivation trials of U. pinnatifida sporophytes increased from 1.8 ± 0.1 cm in November 2021 to 120–170 cm in March 2022 (density, 39.8 plants m⁻¹; final wet weight, 98.6–249.1 g (mean 146.8 ± 20.4 g, n = 20 ind.); yield 5842 g m⁻¹). Further, for the second cultivation trial, the length of the sporophytes increased from 1.5 ± 0.1 cm in November 2021 to 120–150 cm in April 2022 (density, 49.3 plants m⁻¹; final wet weight, 83.0–251.6 g (mean 155.7 ± 19.0 g; n = 20 ind.); yield, 7676 g m⁻¹), and, owing to the increase in water temperature and light intensity due to seasonal changes around the offshore wind power farm, the second cultivation trials showed signs of chlorosis. Considering the environment, we judged seaweed growth to be normal. Therefore, when applying this model to grow U. pinnatifida, seasonal temperature changes, the purpose of the product, and the nutritional status of the open-sea area should be considered. These results may improve seaweed farming in offshore wind farms in the future. Full article

A significant body of controlled laboratory research suggests different biological mechanisms by which the low-cost co-culture of seaweed and shrimp could improve sustainability whilst increasing income for the many poor pond farmers of South-East Asia. However, at the pond level, production and cost–benefit assessments remain largely lacking. Here, we studied the extensive co-culture of Gracilariopsis longissima seaweed and Penaeus monodon shrimp on pond production output, nutrient concentrations, and farm income on the north coast of Java, Indonesia. Co-culture showed 18% higher seaweed production during the first cycle (2261.0 ± 348.0 kg·ha⁻¹) and 27% higher production during the second (2,361.0 ± 127.3 kg·ha⁻¹) compared to monoculture. Shrimp production per cycle was 53.8% higher in co-culture (264.4 ± 47.6 kg·ha⁻¹) than in single-species cultivation (171.7 ± 10.4 kg·ha⁻¹). Seaweed agar content and gel strength did not differ between treatments, and neither did shrimp bacterial or heavy metals concentrations. The profit of co-culture was, respectively, 156% and 318% compared to single-species seaweed and shrimp cultivation. Co-cultivation lowered nutrient loading in the pond water and in the sediment and is argued to be a low-investment and environmentally friendly option for poor pond farmers to improve their income and financial resilience through product diversification. Full article

Ocimum basilicum is an herbaceous plant, rich in essential oils. This research represents a groundbreaking exploration of the cultivation of Ocimum basilicum in Greece, a Mediterranean nation. It emphasizes the impact of biostimulants on various basil varieties, assessing both quantitative aspects and qualitative features. This study was conducted through a field trial at the University of Thessaly’s experimental farm located in the Velestino region. This study examined different testing varieties (V1: Lemon, V2: Siam Queen, V3: Salat, V4: Bascuro, and V5: Genovese), under different biostimulant applications (B1: control, B2: seaweed extracts, amino acids, vitamins, trace elements, polyphenols, antioxidants and mannitol; B3: plant amino acids, glutamic and aspartic acid, vitamins and other nutrients, B4: B1 and B2 combination in a 1:1 ratio). The findings highlight the significant differences in both fresh and dry yields across various basil cultivars, with Lemon basil demonstrating the most substantial yields. Specifically, the Lemon variety attained the highest dry yield, surpassing the lowest-performing cultivar by more than two times. Additionally, this research evaluated the production of essential oil per hectare, emphasizing the relationship between essential oil content and the crop’s dry yield. The results revealed considerable variability among the examined varieties, with the Lemon variety yielding nearly 65 kg ha⁻¹, the highest among them. Biostimulant treatments (B2) led to the greatest total yields of essential oils, while the control treatments yielded the least. The chemical composition of essential oils derived from O. basilicum shows significant variability, often associated with the plants’ nutritional conditions. The application of biostimulants has led to considerable alterations in the volatile profile of sweet basil, supporting this study’s conclusions. Full article

Sustainable seaweed cultivation is crucial for marine environmental protection, ecosystem health, socio-economic development, and carbon sequestration. Accurate and timely information on the distribution, extent, species, and production of cultivated seaweeds is essential for tracking biomass production, monitoring ecosystem health, assessing environmental impacts, optimizing cultivation planning, supporting investment decisions, and quantifying carbon sequestration potential. However, this important information is usually lacking. This study developed a high-precision monitoring approach by integrating Otsu thresholding features with random forest classification, implemented through Google Earth Engine using Sentinel-2 imagery (10-m). The method was applied to analyze spatiotemporal variations of seaweed cultivation across the Korean Peninsula from 2017 to 2023. Results showed that annual cultivation acreage in North Korea remained relatively stable between 1506 and 2033 ha, while it experienced a significant increase of 8209 ha in South Korea. By integrating spectral features, seaweed phenology, and field cultivation practices, we successfully differentiated the predominant species: laver (Pyropia) and kelp (Saccharina and Undaria). During the 2022–2023 cultivation season, South Korea’s farms comprised 78% laver and 22% kelp, while North Korea’s showed an inverse distribution. A strong correlation (r² = 0.99) between acreage and seaweed production enabled us to estimate annual seaweed production in North Korea, effectively addressing data gaps in regions with limited statistics. Our approach demonstrates the potential for global seaweed cultivation monitoring, while the spatial analysis lays the foundation for identifying potential cultivation zones. Given the relatively low initial investment requirement of seaweed farming and significant economic return, this approach offers valuable insights for promoting economic development and food security, ultimately supporting sustainable aquaculture management. Full article

While the free and open-source Broadly Reconfigurable and Expandable Automation Device (BREAD) has demonstrated functionality as an inexpensive replacement for many commercial controllers, some aspects of its design require updating to make it more aligned with commercial supervisory control and data acquisition (SCADA) systems. Some of these updates to BREAD for version 2 included improvements to the mechanical design for stability with an alignment cover, rail mounting with Deutsche Institut für Normung (DIN) rail clips, ESP32 Loaf Controller with local wireless connectivity, and open-source web browser-based software control. These updates were validated by comparing BREAD v2 to an existing commercial controller used for airline-based pH control for industrial seaweed production. BREAD v2 was integrated into an electrical enclosure complete with pH probes, CO₂ lines, solenoid valves, and a power supply. After comparing the two approaches, BREAD v2 was found to be more precise by roughly a factor of five, and less expensive by a factor of three than proprietary systems, while also offering additional functionality like data logging and wireless monitoring. Although able to match or beat specific functions of SCADA systems, future work is needed to transform BREAD into a full SCADA system. Full article