Search Results (2,403)

Fecal microbiota transplantation (FMT) promotes growth performance and intestinal development in yellow-feathered broilers, but whether the virome and metabolites contribute to its growth-promoting effect remains unclear. This study removed the microbiota from FMT filtrate using a 0.45 μm filter membrane, retaining the virome and metabolites to perform fecal virome transplantation (FVT), aiming to investigate its regulatory role in broiler growth. Healthy yellow-feathered broilers with high body weights (top 10% of the population) were used as FVT donors. Ninety-six 8-day-old healthy male yellow-feathered broilers (95.67 ± 3.31 g) served as FVT recipients. Recipient chickens were randomly assigned to a control group and an FVT group. The control group was gavaged with 0.5 mL of normal saline daily, while the FVT group was gavaged with 0.5 mL of FVT solution daily. Growth performance, immune and antioxidant capacity, intestinal development and related gene expression, and microbial diversity were measured. The results showed that FVT improved the feed utilization rate of broilers (the feed conversion ratio decreased by 3%; p < 0.05), significantly increased jejunal length (21%), villus height (69%), and crypt depth (84%) (p < 0.05), and regulated the jejunal barrier: insulin-like growth factor-1 (IGF-1) (2.5 times) and Mucin 2 (MUC2) (63 times) were significantly upregulated (p < 0.05). FVT increased the abundance of beneficial bacteria Lactobacillales. However, negative effects were also observed: Immunoglobulin A (IgA), Immunoglobulin G (IgG), Immunoglobulin M (IgM), Interleukin-1 beta (IL-1β), Interleukin-6 (IL-6), Tumor Necrosis Factor-alpha (TNF-α), and Interferon-gamma (IFN-γ) in broilers were significantly upregulated (p < 0.05), indicating immune system overactivation. Duodenal barrier-related genes Mucin 2 (MUC2), Occludin (OCLN), Claudin (CLDN1), and metabolism-related genes solute carrier family 5 member 1 (SLC5A1) and solute carrier family 7 member 9 (SLC7A9) were significantly downregulated (p < 0.05). The results of this trial demonstrate that, besides the microbiota, the gut virome and metabolites are also functional components contributing to the growth-promoting effect of FMT. The differential responses in the duodenum and jejunum reveal spatial heterogeneity and dual effects of FVT on the intestine. The negative effects limit the application of FMT/FVT. Identifying the primary functional components of FMT/FVT to develop safe and targeted microbial preparations is one potential solution. Full article

This paper presents the design, fabrication, and experimental evaluation of a capacitive micromachined ultrasonic transducer (CMUT) linear array for non-contact thickness measurement of marine engineering structures. A 16-element CMUT array was fabricated using a silicon–silicon wafer bonding process, and encapsulated in polyurethane to ensure acoustic impedance matching and environmental protection in underwater conditions. The acoustic performance of the encapsulated CMUT was characterized using standard piezoelectric transducers as reference. The array achieved a transmitting sensitivity of 146.82 dB and a receiving sensitivity of −229.55 dB at 1 MHz. A complete thickness detection system was developed by integrating the CMUT array with a custom transceiver circuit and implementing a time-of-flight (ToF) measurement algorithm. To evaluate environmental robustness, systematic experiments were conducted under varying water temperatures and salinity levels. The results demonstrate that the absolute thickness measurement error remains within ±0.1 mm under all tested conditions, satisfying the accuracy requirements for marine structural health monitoring. The results validate the feasibility of CMUT-based systems for precise and stable thickness measurement in underwater environments, and support their application in non-destructive evaluation of marine infrastructure. Full article

In this case study, we used the Delta Simulation Model II (DSM2) to study the salt balance at the land–water interface in the river delta of California’s San Francisco Estuary. Drainage, a source of water and salt for adjacent channels in the study area, is affected by channel salinity. The DSM2 approach has been adopted by several hydrodynamic models of the estuary to enforce water volume balance between diversions, evapotranspiration and drainage at the land–water interface, but does not explicitly enforce salt balance. We found deviations from salt balance to be quite large, albeit variable in magnitude due to the heterogeneity of hydrodynamic and salinity conditions across the study area. We implemented a procedure that approximately enforces salt balance through iterative updates of the baseline drain salinity boundary conditions (termed loose coupling). We found a reasonable comparison with field measurements of drainage salinity. In particular, the adjusted boundary conditions appear to capture the range of observed interannual variability better than the baseline periodic estimates. The effect of the iterative adjustment procedure on channel salinity showed substantial spatial variability: locations dominated by large flows were minimally impacted, and in lower flow channels, deviations between baseline and adjusted channel salinity series were notable, particularly during the irrigation season. This approach, which has the potential to enhance the simulation of extreme salinity intrusion events (when high channel salinity significantly impacts drainage salinity), is essential for robustly modeling hydrodynamic conditions that pre-date contemporary water management infrastructure. We discuss limitations associated with this approach and recommend that—for this case study—further improvements could best be accomplished through code modification rather than coupling of transport and island water balance models. Full article

Objective: To investigate the neuroprotective effects of a Rho-associated kinase (ROCK) inhibitor on retinal ganglion cells (RGCs) in vitro and in vivo. Methods: For in vivo studies, a unilateral optic nerve crush mouse model was established. Then, 100 mM Y-27632 (a ROCK inhibitor) or saline was applied to the experimental eyes once a day for 14 days. The effects of the ROCK inhibitor were evaluated by counting the surviving RGCs in the enucleated flat retina tissues and measuring the inner retinal thickness using optical coherence tomography (OCT), the amplitude of the electroretinogram (ERG), and the change in intraocular pressure (IOP). For the in vitro study, RGCs were isolated from five-day-old mice using a modified immunopanning method with magnetic beads. The isolated RGCs were incubated for 72 h with various concentrations of Y-27632, after which TUNEL assays were performed to determine the number of surviving RGCs. Results: Y-27632 has neuroprotective effects, as it significantly increased the number of surviving RGCs by approximately 6.3%. OCT and ERG data also revealed that Y-27632 induced neuroprotective effects in vivo; furthermore, Y-27632 reduced IOP by approximately 18.3%. The in vitro study revealed the dose-dependent neuroprotective effects of Y-27632, with the highest dose of Y-27632 (1000 nM) increasing the RGC survival rate after 72 h of incubation compared with that of the control. Conclusions: The ROCK inhibitor Y-27632 may exert some neuroprotective effects on RGCs when it is used as an eye drop through an IOP-independent mechanism. Full article

Water scarcity challenges floriculture, which depends on quality irrigation for ornamental value. This study assessed short-term salinity tolerance in eight Asteraceae species by measuring physiological (proline levels, antioxidant enzyme activity) and morphological (plant height, flower number, and size) responses. Plants were irrigated with 0, 50, 100, or 300 mM NaCl for 10 days. Salinity significantly enhanced proline content and the activity of key antioxidant enzymes (catalase, peroxidase, and ascorbate peroxidase), reflecting the activation of stress defense mechanisms. However, these defenses failed to fully protect reproductive organs. Flower number and size were consistently more sensitive to salinity than vegetative traits, with significant reductions observed even at 50 mM NaCl. Responses varied between species, with Zinnia elegans and Calendula officinalis exhibiting pronounced sensitivity to salinity, whereas Tagetes patula showed relative tolerance, particularly under moderate stress conditions. The results show that flower structures are more vulnerable to ionic and osmotic disturbances than vegetative tissues, likely due to their higher metabolic demands and developmental sensitivity. Their heightened vulnerability underscores the need to prioritize reproductive performance when evaluating stress tolerance. Incorporating these traits into breeding programs is essential for developing salt-tolerant floriculture species that maintain aesthetic quality under limited water availability. Full article

The salinization of agricultural soils is a serious threat to farming and ecological balance in arid and semi-arid regions. Accurate estimation of soil water-soluble ions (calcium, carbonate, magnesium, and sulfate) is necessary for correct monitoring of soil salinization and sustainable land management. Hyperspectral ground-based data are valuable in soil salinization monitoring, but the acquisition cost is high, and the coverage is small. Therefore, this study proposes a two-stage deep learning framework with multispectral remote-sensing images. First, the wavelet transform is used to enhance the Transformer and extract fine-grained spectral features to reconstruct the ground-based hyperspectral data. A comparison of ground-based hyperspectral data shows that the reconstructed spectra match the measured data in the 450–998 nm range, with R² up to 0.98 and MSE = 0.31. This high similarity compensates for the low spectral resolution and weak feature expression of multispectral remote-sensing data. Subsequently, this enhanced spectral information was integrated and fed into a novel multiscale self-attentive Transformer model (MSATransformer) to invert four water-soluble ions. Compared with BPANN, MLP, and the standard Transformer model, our model remains robust across different spectra, achieving an R² of up to 0.95 and reducing the average relative error by more than 30%. Among them, for the strongly responsive ions magnesium and sulfate, R² reaches 0.92 and 0.95 (with RMSE of 0.13 and 0.29 g/kg, respectively). For the weakly responsive ions calcium and carbonate, R² stays above 0.80 (RMSE is below 0.40 g/kg). The MSATransformer framework provides a low-cost and high-accuracy solution to monitor soil salinization at large scales and supports precision farmland management. Full article

Owing to the inherently lower sensitivity of microwave aperture synthesis radiometers (ASRs), Soil Moisture and Ocean Salinity (SMOS) satellite brightness temperature (TB) measurements exhibit significantly greater system noise than real-aperture microwave radiometers do. This paper introduces a novel noise mitigation method for the SMOS L1C multi-angle TB product. The proposed method develops a multi-angle sea surface TB relationship lookup table, enabling the mapping of SMOS L1C multi-angle TB data to any single-angle TB, thereby averaging to the measurements to reduce noise. Validation experiments demonstrate that the processed SMOS TB data achieve noise levels comparable to those of the Soil Moisture Active Passive (SMAP) satellite. Additionally, the salinity retrieval experiments indicate that the noise mitigation technique has a clear positive effect on SMOS salinity retrieval. Full article

Diabetic wounds are a devastating complication that cause chronic pain, recurrent infections, and limb amputations due to impaired healing. Despite advances in wound care, existing therapies often fail to address the underlying molecular dysregulation, highlighting the need for innovative and safe therapeutic approaches. Among these, D-amino acids such as D-tryptophan (D-Trp) have emerged as key regulators of cellular processes; however, their therapeutic potential in diabetic wounds remains largely unexplored. Here, we investigate the therapeutic potential of D-Trp in streptozotocin (STZ)-induced diabetic mice, comparing it with phosphate-buffered saline (PBS) controls and vascular endothelial growth factor (VEGF) as a positive control. Wound healing, inflammation, and histopathology were assessed. Protein and gene expression were analyzed via Western blot and RT-qPCR, respectively. Biolayer interferometry (BLI) measured the binding of D-Trp to hypoxia-inducible factor-1α (HIF-1α). D-Trp accelerated wound healing by modulating extracellular matrix (ECM) remodeling, signaling, and apoptosis. It upregulated matrix metalloproteinases (MMP1, MMP3, MMP-9), Janus kinase 2 (JAK2), and mitogen-activated protein kinase (MAPK) proteins while reducing pro-inflammatory cytokines (tumor necrosis factor-α [TNF-α], interleukin-1β [IL-1β], IL-6). D-Trp also suppressed caspase-3 and enhanced angiogenesis through HIF-1α activation. These findings suggest that D-Trp promotes healing by boosting ECM turnover, reducing inflammation, and activating MAPK/JAK pathways. Thus, D-Trp is a promising therapeutic for diabetic wounds. Full article

Salinity intrusion is a growing threat to freshwater resources, particularly in low-lying coastal and estuarine regions, necessitating the development of effective early warning systems (EWS) to support timely mitigation. Although various water quality monitoring technologies exist, many face challenges related to long-term sustainability, ongoing maintenance, and accessibility for local users. This study introduces a novel hybrid real-time salinity intrusion early warning system that uniquely integrates fixed and portable monitoring technologies with strong community participation—an approach not yet widely applied in comparable urban-adjacent delta regions. Unlike traditional systems, this model emphasizes local ownership, flexible data collection, and system scalability in resource-constrained environments. This study presents a real-time salinity intrusion early warning system for Bang Kachao, Thailand, combining eight fixed monitoring stations and 20 portable salinity measurement devices. The system was developed in response to community needs, with local input guiding both station placement and the design of mobile measurement tools. By integrating fixed stations for continuous, high-resolution data collection with portable devices for flexible, on-demand monitoring, the system achieves comprehensive spatial coverage and adaptability. A core innovation lies in its emphasis on community participation, enabling villagers to actively engage in monitoring and decision-making. The use of IoT-based sensors, Remote Telemetry Units (RTUs), and cloud-based data platforms further enhances system reliability, efficiency, and accessibility. Automated alerts are issued when salinity thresholds are exceeded, supporting timely interventions. Field deployment and testing over a seven-month period confirmed the system’s effectiveness, with fixed stations achieving 90.5% accuracy and portable devices 88.7% accuracy in detecting salinity intrusions. These results underscore the feasibility and value of a hybrid, community-driven monitoring approach for protecting freshwater resources and building local resilience in vulnerable regions. Full article

The use of chloride-based deicing salts, particularly sodium chloride (NaCl) and calcium chloride (CaCl₂), is a common practice in cold regions for maintaining road safety during winter. However, the accumulation of salt residues in adjacent soils poses serious environmental threats, including reduced pH, increased electrical conductivity (EC), disrupted soil structure, and plant growth inhibition. This study aimed to evaluate the combined effect of activated carbon (AC) and Pennisetum alopecuroides, a salt-tolerant perennial grass, in alleviating salinity stress under deicer-treated soils. A factorial greenhouse experiment was conducted using three fixed factors: (i) presence or absence of Pennisetum alopecuroides, (ii) deicer type (NaCl or CaCl₂), and (iii) activated carbon mixing ratio (0, 1, 2, 5, and 10%). Soil pH, EC, and ion concentrations (Na⁺, Cl⁻, Ca²⁺) were measured, along with six plant growth indicators. The results showed that increasing AC concentrations significantly increased pH and reduced EC and ion accumulation, with the 5% AC treatment being optimal in both deicer systems. Plant physiological responses were improved in AC-amended soils, especially under CaCl₂ treatment, indicating less ion toxicity and better root zone conditions. The interaction effects between AC, deicer type, and plant presence were statistically significant (p < 0.05), supporting a synergistic remediation mechanism involving both adsorption and biological uptake. Despite the limitations of short-term controlled conditions, this study offers a promising phytomanagement strategy using natural adsorbents and salt-tolerant plants for sustainable remediation of salt-affected soils in road-adjacent and urban environments. Full article

Introduction: The important factor in applying substances for inner ear therapy is the atraumatic execution, as well as effective concentration uniformly distributed in all regions of the cochlea within a reasonable time frame. This study investigates whether an additional cochlear opening (“second-hole technique”) can improve fluid distribution and reduce intracochlear pressure during dye delivery into the cochlear models and human petrous bone. Material and Methods: Three experimental setups were used: an uncoiled scala tympani model, a full-scale 3D-printed cochlear model, and a human petrous bone. In all cases, 1% methylene blue-stained saline was infused using a cochlear catheter (MED-EL, Innsbruck, Austria) through the round window. Intracochlear pressure was measured via fiberoptic pressure sensors inserted through a burr hole (artificial cochlear models) or at the lateral semicircular canal (human petrous bone). A second hole was made on the helicotrema in the inner ear models or at the oval window of the human petrous bone to examine the effect of a second hole on intracochlear pressure and fluid distribution. Dye distribution and intracochlear pressure were measured in 3D artificial models at two flow rates (0.2 and 0.4 mL/h). The intracochlear pressure were measured in the human petrous bone at a fixed rate (0.4 mL/h). Results: The use of a second hole significantly improved dye distribution in 3D models at both flow rates (p < 0.05) and led to earlier saturation-level distribution. Intracochlear pressure remained significantly lower and more stable in models with a second hole (p < 0.05). In human petrous bones, pressure fluctuation was reduced by the second hole, though pressure still increased over time. Conclusions: Using a second-hole technique leads to a faster, uniform level of dye distribution throughout the cochlear models, as well as a lower intracochlear pressure, which can be assumed to be an essential factor for hearing preservation during dye application. Full article

This dataset presents environmental observations collected in August 2021 from 18 port and harbor sites located around Jeju Island, Korea. It includes physical, biogeochemical, and greenhouse gas (GHG) variables measured in surface seawater, such as temperature, salinity, dissolved oxygen, nutrients, chlorophyll-a, pH, total alkalinity, and dissolved inorganic carbon. Concentrations and air–sea fluxes of nitrous oxide (N₂O), methane (CH₄), and carbon dioxide (CO₂) were also quantified. All measurements were conducted following standardized analytical protocols, and certified reference materials and duplicate analyses were used to ensure data accuracy. Consequently, the dataset revealed that elevated nutrient accumulation in port and harbor waters and GHG concentrations tended to be higher at sites with stronger land-based influence. During August 2021, most sites functioned as sources of N₂O, CH₄, and CO₂ to the atmosphere. This integrated dataset offers valuable insights into the influence of anthropogenic and hydrological factors on coastal GHG dynamics and provides a foundation for future studies across diverse semi-enclosed marine systems. Full article

The present study was conducted in Dacope Upazila, a sub-district located within the Khulna District of the coastal region in Bangladesh. The research methods employed included the implementation of 350 household questionnaire surveys (HQSs), 12 focus group discussions (FGDs), and 20 key informant interviews (KIIs) to assess economic security status in disaster-vulnerable areas. The findings indicate that the economic well-being of the region is precarious due to a paucity of revenue sources and the occurrence of various calamitous events, induced risks, and vulnerabilities. To achieve long-term economic security for households, a considerable proportion of the population (approximately 22%) in the study areas is dependent on agricultural activities for their livelihoods. The study also revealed that approximately 22% of households in the study areas reported experiencing salinity intrusion. Furthermore, most of the households, around 68%, reported cyclones as their primary obstacle to building disaster-resilient communities. Consequently, the prevailing local and institutional strategies to ensure economic security were found to be inadequate and unsustainable in the study upazila. Therefore, the study resulted in the formulation of a conceptual framework intended to measure the contribution of economic security to the adaptability and sustainability of disaster-resilient communities in vulnerable coastal areas of Bangladesh. Full article

Background/Objectives: The accurate localization of interfascial planes is critical for effective regional anesthesia, yet current techniques relying on ultrasound guidance can be challenging, especially in obese or pediatric patients. Previous cadaveric and clinical studies have suggested that injection pressure varies depending on needle placement relative to fascial and neural structures. This pilot study aimed to evaluate whether the continuous monitoring of injection pressure can reliably differentiate interfascial spaces from surrounding anatomical structures in a porcine tissue model. Methods: A custom-built pressure monitoring system was used to continuously measure saline injection pressure during regional block procedures performed on porcine thighs. Injections were guided by ultrasound and conducted using an infusion pump. Needle positions were classified as intramuscular, resting on fascia, or interfascial. Statistical comparisons of pressure levels, variability, and temporal trends were conducted using Wilcoxon signed-rank tests and regression analysis. Results: Mean intramuscular pressure was significantly higher than the mean interfascial pressure (p < 1 × 10⁻¹³). Interfascial injections demonstrated lower pressure variability (p = 2.1 × 10⁻⁴) and an increasing trend in pressure over time (p = 2.1 × 10⁻⁴), whereas intramuscular injections exhibited a decreasing pressure trend (p = 3.15 × 10⁻³). Conclusions: Continuous pressure monitoring effectively distinguishes interfascial from intramuscular and fascial penetration phases during regional anesthesia. The method demonstrates potential as a real-time, objective tool for enhancing needle guidance and improving the safety and accuracy of interfascial plane blocks. Further cadaveric and clinical studies are warranted to validate these findings. Full article

Accurate estimation of winter wheat yield under saline stress is crucial for addressing food security challenges and optimizing agricultural management in regional soils. This study proposed a method integrating Sentinel-2 data and field-measured soil salt content (SC) using a random forest (RF) method to improve yield estimation of winter wheat in Kenli County, a typical saline area in China’s Yellow River Delta. First, feature importance analysis of a temporal vegetation index (VI) and salinity index (SI) across all growth periods were achieved to select main parameters. Second, yield models of winter wheat were developed in VI-, SI-, VI + SI-, and VI + SI + SC-based groups. Furthermore, error assessment and spatial yield mapping were analyzed in detail. The results demonstrated that feature importance varied by growth periods. SI dominated in pre-jointing periods, while VI was better in the post-jointing phase. The VI + SI + SC-based model achieved better accuracy (R² = 0.78, RMSE = 720.16 kg/ha) than VI-based (R² = 0.71), SI-based (R² = 0.69), and VI + SI-based (R² = 0.77) models. Error analysis results suggested that the residuals were reduced as the input parameters increased, and the VI + SI + SC-based model showed a good consistency with the field-measured yields. The spatial distribution of winter wheat yield using the VI + SI + SC-based model showed significant differences, and average yields in no, slight, moderate, and severe salinity areas were 7945, 7258, 5217, and 4707 kg/ha, respectively. This study can provide a reference for winter wheat yield estimation and crop production improvement in saline regions. Full article