Search Results (1,019)

The 13th International Foamy Virus (FV) Conference was held from 8 to 10 November 2023 at the BioParque/Zoological Garden in Rio de Janeiro, Brazil. This was the first conference on spumaretroviruses to be held in the Southern Hemisphere and in the unique environment of the rainforest. New developments and current perspectives in FV research were presented. Highlights of the conference included the structural biology of the envelope protein (Env) and insights into its function and evolution, epidemiologic identification of Amazonian indigenous people with a high prevalence of simian FV (SFV) infections, investigations of virus biology and genomics using synthetic FV DNAs, studies of humoral immune response, and development and applications of SFV vectors. The last day of the meeting was a special tour of the Centro de Primatologia do Rio de Janeiro, located northeast of Rio de Janeiro amidst the protected rainforest, where New World primate hosts of spumaretroviruses are rescued and studied. Our report summarizes the meeting highlights and outcomes for future discussions. Full article

Tall-pier girder bridges with fluid viscous dampers (FVDs) are widely used in earthquake-prone mountainous areas. However, the influence of higher-order modes and near-field earthquakes on tall piers has rarely been studied. Based on an asymmetric tall-pier girder bridge, a finite element model is established, and the parameters of FVDs are optimized using SAP2000. The higher-order mode effects on tall piers are explored by proportionally reducing the pier heights. The pulse effects of near-field earthquakes on FVD mitigation and higher-order modes are analyzed. The optimal FVDs can coordinate the force distribution among tall piers, effectively reducing displacement responses and internal forces. Due to higher-order modes, the internal force envelopes of tall piers exhibit concave-convex distributions. As pier heights decrease, the internal force envelopes gradually become linear, implying reduced higher-order mode effects. Long-period pulse-like motions produce the maximum seismic responses because the slender tall-pier bridge is sensitive to high spectral accelerations in medium-to-long periods. The higher-order modes are more easily excited by near-field motions with large spectral values in the high-frequency range. Overall, FVDs can simultaneously reduce the seismic responses of tall piers and diminish the influence of higher-order modes. Full article

Early evacuation during bushfires remains the safest strategy; however, in many realistic scenarios, timely evacuation is challenging, making safe sheltering a last-resort option to reduce risk compared to late evacuation attempts. However, most Australian homes in bushfire-prone areas are neither designed nor retrofitted to provide adequate protection against extreme bushfires, raising safety concerns. This study addresses this gap by investigating the concept of retrofitting a part of the residential buildings as attached safe rooms for sheltering and protection of valuables, providing a potential last-resort solution for bushfire-prone communities. Numerical simulations were conducted using the Fire Dynamics Simulator to assess heat transfer and internal temperature conditions in a representative residential building under bushfire exposure conditions. The study investigated the impact of the placement of the safe room relative to the fire front direction, failure of vulnerable building components, and the effectiveness of steel shutters in response to internal temperatures. The results showed that the strategic placement of safe rooms inside the building, along with adequate protective measures for windows, can substantially reduce internal temperatures. The findings emphasised the importance of maintaining the integrity of openings and the external building envelope, demonstrating the potential of retrofitted attached safe rooms as a last-resort solution for existing residential buildings in bushfire-prone areas where the entire building was not constructed to withstand bushfire conditions. Full article

This study develops a novel transverse steel damper (TSD) to enhance the seismic performance of tall-pier girder bridges, featuring superior lateral strength and energy dissipation capacity. The TSD’s design and arrangement are presented, with its hysteretic behavior simulated in ABAQUS. Key parameters (yield strength: 3000 kN; initial gap: 100 mm; post-yield stiffness ratio: 15%) are optimized through seismic analysis under near-fault ground motions, incorporating pulse characteristic investigations. The optimized TSD effectively reduces bearing displacements and results in smaller pier top displacements and internal forces compared to the bridge with fixed bearings. Due to the higher-order mode effects, there is no direct correlation between top displacements and bottom internal forces. As pier height decreases, the S-shaped shear force and bending moment envelopes gradually become linear, reflecting the reduced influence of these modes. Medium- to long-period pulse-like motions amplify seismic responses due to resonance (pulse period ≈ fundamental period) or susceptibility to large low-frequency spectral values. Higher-order mode effects on bending moments and shear forces intensify under prominent high-frequency components. However, the main velocity pulse typically masks the influence of high-order modes by the overwhelming seismic responses due to large spectral values at medium to long periods. Full article

The global antimicrobial resistance crisis demands innovative strategies to combat bacterial infections, including those caused by drug-sensitive pathogens that evade treatment through biofilm formation or metabolic adaptations. Here, we demonstrate that Squama Manitis extract (SME)—a traditional Chinese medicine component—exhibits broad-spectrum bactericidal activity against clinically significant pathogens, including both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) species (MIC = 31.25 mg/mL), achieving significant reduction in bacterial viability within 24 h. Through integrated multi-omics analysis combining scanning electron microscopy and RNA sequencing, we reveal SME’s unprecedented tripartite mechanism of action: (1) direct membrane disruption causing cell envelope collapse, (2) metabolic paralysis through coordinated suppression of TCA cycle and fatty acid degradation pathways, and (3) inhibition of DNA repair systems (SOS response and recombination downregulation). Despite its potent activity, SME shows low cytotoxicity toward mammalian cells (>90% viability) and can penetrate Gram-negative outer membranes. These features highlight SME’s potential to address drug-resistant infections through synthetic lethality across stress response, energy metabolism, and DNA integrity pathways. While advocating for synthetic alternatives to endangered animal products, this study establishes SME as a polypharmacological template for resistance-resilient antimicrobial design, demonstrating how traditional knowledge and modern systems biology can converge to guide sustainable anti-infective development. Full article

The production of thermal energy in buildings using internal greenhouses makes it possible to obtain substantial gains in energy consumption and, at the same time, contribute to improving occupants’ thermal comfort (TC) levels. This article proposes a study on the producing and transporting of renewable thermal energy in a circular auditorium equipped with an enveloping semi-circular greenhouse. The numerical study is based on software that simulates the building geometry and the building thermal response (BTR) numerical model and assesses the TC level and indoor air quality (IAQ) provided to occupants in spaces ventilated by the proposed system. The building considered in this study is a circular auditorium constructed from three semi-circular auditoriums supplied with internal semi-circular greenhouses. Each of the semi-circular auditoriums faces south, northeast, and northwest, respectively. The semi-circular auditoriums are occupied by 80 people each: the one facing south throughout the day, while the one facing northeast is only occupied in the morning, and the one facing northwest is only occupied in the afternoon. The south-facing semi-circular greenhouse is used by itself to heat all three semi-circular auditoriums. The other two semi-circular greenhouses are only used to heat the interior space of the greenhouse. It was considered that the building is located in a Mediterranean-type climate and subject to the typical characteristics of clear winter days. The results allow us to verify that the proposed heating system, in which the heat provided to the occupied spaces is generated only in the semi-circular greenhouse facing south, can guarantee acceptable TC conditions for the occupants throughout the occupancy cycle. Full article

The construction industry is one of the main areas of energy consumption and carbon emissions, and strengthening research on the thermal performance of building facades can effectively promote energy conservation and emission reduction. Compared with traditional static enclosure structures, dynamic skin can adapt its functions, characteristics, and methods based on constantly changing environmental conditions and performance requirements. It has great potential in adapting to the environment, reducing energy consumption, adjusting shading and natural ventilation, and improving human thermal and visual comfort. To comprehensively understand the key technologies of dynamic skin energy-saving design, previous research results were comprehensively compiled from relevant databases. The research results indicate that various types of dynamic skins, intelligent materials, multi-layer facades, dynamic shading, and biomimetic facades are commonly used core technologies for dynamic facades. Parametric modeling, computer simulation, and multi-objective algorithms are commonly used to optimize the performance of dynamic skin. In addition, integrated technology design, interaction design, and lifecycle design should be effective methods for improving dynamic skin energy efficiency, resident satisfaction, and economic benefits. Despite current challenges, dynamic skin energy-saving technology remains one of the most effective solutions for future sustainable building design. Full article

The 2016 Zika virus (ZIKV) epidemic has largely subsided, but a key question remains. How did ZIKV evolve to become a virulent human pathogen compared to the virus of its original discovery? What specific virologic and pathologic changes contributed to increased pathogenicity in humans? Phylogenetic studies have identified two genetically distinct ZIKV, the African and Asian lineages, which differ in their pathogenicity. Previous studies including ours suggest that the envelope (E) protein plays a key role in viral entry, immune activation, and neuropathogenesis. This study aimed to further elucidate virologic and pathogenic differences between these lineages by assessing their ability to bind and replicate in host cells, induce apoptotic cell death, trigger inflammatory responses, and influence human neural progenitor cell (hNPC)-derived neurosphere formation. We compared a historic African ZIKV strain (MR766) with an epidemic Brazilian strain (BR15) and evaluated the effects of the E protein inhibitor quercetin-3-β-O-D-glucoside (Q3G) and an E protein-neutralizing antibody (AbII). Our results revealed distinct virologic properties and that MR766 exhibited stronger inhibition of neurosphere formation due to enhanced viral binding to neuronal SH-SY5Y cells, while BR15 infection triggered a heightened pro-inflammatory cytokine response with reduced viral binding. Chimeric virus studies suggested that the E protein likely influences viral binding, replication efficiency, immune activation, and neuropathogenesis. Notably, Q3G exhibited antiviral activities against both MR766 and BR15, whereas AbII preferentially inhibited MR766. These findings highlight the virological differences between ancestral and epidemic viral strains, as well as the critical role of E protein in viral permissiveness, immune response, and neuropathogenesis, providing insights for developing targeted antiviral strategies. Full article

The construction sector presently consumes about 40% of global energy and generates 36% of CO₂ emissions, making facade retrofits a priority for decarbonising buildings. This review clarifies how ventilated facades (VFs), wall assemblies that interpose a ventilated air cavity between outer cladding and the insulated structure, address that challenge. First, the paper categorises VFs by structural configuration, ventilation strategy and functional control into four principal families: double-skin, rainscreen, hybrid/adaptive and active–passive systems, with further extensions such as BIPV, PCM and green-wall integrations that couple energy generation or storage with envelope performance. Heat-transfer analysis shows that the cavity interrupts conductive paths, promotes buoyancy- or wind-driven convection, and curtails radiative exchange. Key design parameters, including cavity depth, vent-area ratio, airflow velocity and surface emissivity, govern this balance, while hybrid ventilation offers the most excellent peak-load mitigation with modest energy input. A synthesis of simulation and field studies indicates that properly detailed VFs reduce envelope cooling loads by 20–55% across diverse climates and cut winter heating demand by 10–20% when vents are seasonally managed or coupled with heat-recovery devices. These thermal benefits translate into steadier interior surface temperatures, lower radiant asymmetry and fewer drafts, thereby expanding the hours occupants remain within comfort bands without mechanical conditioning. Climate-responsive guidance emerges in tropical and arid regions, favouring highly ventilated, low-absorptance cladding; temperate and continental zones gain from adaptive vents, movable insulation or PCM layers; multi-skin adaptive facades promise balanced year-round savings by re-configuring in real time. Overall, the review demonstrates that VFs constitute a versatile, passive-plus platform for low-carbon buildings, simultaneously enhancing energy efficiency, durability and indoor comfort. Future advances in smart controls, bio-based materials and integrated energy-recovery systems are poised to unlock further performance gains and accelerate the sector’s transition to net-zero. Emerging multifunctional materials such as phase-change composites, nanostructured coatings, and perovskite-integrated systems also show promise in enhancing facade adaptability and energy responsiveness. Full article

The commercialization of selective broccoli harvesters, a critical response to agricultural labor shortages, is hampered by end effectors with large operational envelopes and poor adaptability to complex field conditions. To address these limitations, this study developed and evaluated a novel end-effector with an integrated transverse cutting mechanism and a foldable grasping cavity. Unlike conventional fixed cylindrical cavities, our design utilizes actuated grasping arms and a mechanical linkage system to significantly reduce the operational footprint and enhance maneuverability. Key design parameters were optimized based on broccoli morphological data and experimental measurements of the maximum stem cutting force. Furthermore, dynamic simulations were employed to validate the operational trajectory and ensure interference-free motion. Field tests demonstrated an operational success rate of 93.33% and a cutting success rate of 92.86%. The end effector successfully operated in dense planting environments, effectively avoiding interference with adjacent broccoli heads. This research provides a robust and promising solution that advances the automation of broccoli harvesting, paving the way for the commercial adoption of robotic harvesting technologies. Full article

The global push for sustainable building practices has intensified the search for low-carbon, recyclable alternatives to traditional roofing materials. This study investigated the structural viability of corrugated panels fabricated from 100% post-consumer recycled HDPE and PP for roofing and cladding applications under real-world loading and environmental conditions. Promising main attributes include durability, corrosion resistance, and low environmental impact. Mechanical testing revealed a flexural strength of 8.4 MPa for rHDPE and 6.3 MPa for rPP. Under impact loading, rPP retained 53% of its initial strength, while rHDPE retained 28%, as validated by drop-weight and pendulum impact tests. Vibration testing (ASTM E1876) demonstrated that rPP exhibited 18% higher longitudinal damping, whereas rHDPE outperformed in out-of-plane vibration control. XRD and SEM-EDS confirmed distinct crystalline and morphological structures responsible for the observed behavior. Findings from this investigation, supported by prototype slab testing, confirm that integrating recycled plastics facilitates the creation of durable and sustainable building envelopes for circular construction practices. Full article

To address the challenges of extracting rolling bearing degradation information and the insufficient performance of conventional convolutional networks, this paper proposes a rolling bearing degradation state identification method based on the improved monopulse feature extraction and a one-dimensional dilated residual convolutional neural network (1D-DRCNN). First, the fault pulse envelope waveform features are extracted through phase scanning and synchronous averaging, and a two-stage grid search strategy is employed to achieve FCC calibration. Subsequently, a 1D-DRCNN model is constructed to identify rolling bearing degradation states under different working conditions. The experimental study collects the vibration signals of nine degradation states, including the different sizes of inner and outer ring local faults as well as normal conditions, to comparatively analyze the proposed method’s rapid calibration capability and feature extraction quality. Furthermore, t-SNE visualization is utilized to analyze the network response to bearing degradation features. Finally, the degradation state identification performance across different network architectures is compared in pattern recognition experiments. The results show that the proposed improved feature extraction method significantly reduces the iterative calibration computational burden while effectively extracting local fault degradation information and overcoming complex working condition influence. The established 1D-DRCNN model integrates the advantages of dilated convolution and residual connections and can deeply mine sensitive features and accurately identify different bearing degradation states. The overall recognition accuracy can reach 97.33%. Full article

The European Union’s (EU) Common Agricultural Policy aims to promote sustainable farming practices that ensure the responsible use of natural resources, safeguard biodiversity, and uphold higher animal welfare standards. One pathway to achieving these objectives is through the encouragement of extensive farming. However, the dairy sector in EU countries as well as in Lithuania has shown a clear trend toward intensification. The aim of this study was to assess the technical efficiency (TE) of dairy farms employing extensive and intensive technologies. TE was evaluated using Data Envelopment Analysis (DEA) combined with meta-frontier analysis, which accounts for technological heterogeneity. Prior to the efficiency estimation, farms were grouped into two distinct categories—intensive and extensive—using the k-means clustering algorithm. The empirical results show that extensive dairy farms in Lithuania are smaller in land area and livestock units, rely more on internal resources, and exhibit lower productivity compared to intensive farms. Intensive farms achieved higher technical efficiency, narrower technological gaps, and more optimal scale efficiency, indicating superior resource management. The weaker performance of extensive farms is attributed to both less advanced technologies and production inefficiencies. Full article

The constitutive model significantly influences the accuracy of predicting the complex rheological behavior of hot isostatically pressed powders. The temperature plays a crucial role in determining material properties during hot isostatic pressing (HIP), making it essential to account for its effect on the yield model parameters to more accurately describe the densification evolution of powders. In this study, HIP experiments were conducted using two different process schemes, and the shrinkage deformation of the envelope under each scheme was analyzed. High-temperature uniaxial compression experiments were performed on HIP samples with varying densities to analyze and characterize the stress–strain response of the powder during HIP. A mesoscopic particle-scale high-temperature uniaxial compression model was developed based on the discrete element method (DEM), and the strain and stress values corresponding to different densities in the high-temperature uniaxial compression simulations were validated through experimental comparison. The strain evolution during the uniaxial compression process was analyzed, and the relationship between the parameters of the Shima–Oyane model and the temperature was established, leading to the development of a temperature-compensated Shima–Oyane model. Based on the obtained parameters at various densities and temperatures, a yield stress map for the nickel-based alloy was constructed. The accuracy of this model was verified by comparing experimental results with finite element method (FEM) simulations. The findings of this study contribute to a more precise prediction of densification behavior in thermally driven isostatic pressing. Full article

Introduction: Genetic plasticity and adaptive camouflage in critical pathogens have contributed to the global surge in multidrug-resistant (MDR) infections, posing a serious threat to public health and therapeutic efficacy. Antimicrobial resistance, now a leading cause of global mortality, demands urgent action through diagnostics, vaccines, and therapeutics. In India, the Indian Council of Medical Research’s surveillance network identifies Escherichia coli as a major cause of urinary tract infections, with increasing prevalence in human gut microbiomes, highlighting its significance across One Health domains. Methods: Whole-genome sequencing of E. coli strain ECG015, isolated from a human gut sample, was performed using the Illumina NextSeq platform. Results: Genomic analysis revealed multiple antibiotic resistance genes, virulence factors, and efflux pump components. Phylogenomic comparisons showed close relatedness to pathovars from both human and animal origins. Notably the genome encoded protein tyrosine kinases (Etk/Ptk and Wzc) and displayed variations in the envelope stress-responsive CpxAR two-component system. Promoter analysis identified putative CpxR-binding sites upstream of genes involved in resistance, efflux, protein kinases, and the MazEF toxin–antitoxin module, suggesting a potential regulatory role of CpxAR in stress response and persistence. Conclusions: This study presents a comprehensive genomic profile of E. coli ECG015, a gut-derived isolate exhibiting clinically significant resistance traits. For the first time, it implicates the CpxAR two-component system as a potential central regulator coordinating antimicrobial resistance, stress kinase signaling, and programmed cell death. These findings lay the groundwork for future functional studies aimed at targeting stress-response pathways as novel intervention strategies against antimicrobial resistance. Full article