Search Results (32,381)

Background: Antimicrobial resistance is a One Health problem driven by the intricate interactions across human, animal, and environmental interfaces that enable microbial exchange and movement of mobile genetic elements encoding resistance and virulence. This study investigated the genomic epidemiology of ESBL and non-ESBL Escherichia coli across One Health interfaces in Oman. Methods: This prospective cross-sectional study analyzed 295 non-duplicate Escherichia coli isolates derived from 104 clinical, 173 animal [diseased (123) and healthy (50)], 14 sewage and four water sources. Antimicrobial susceptibility testing was performed phenotypically, and a representative subset of 50 ESBL and non-ESBL Escherichia coli from the three interfaces underwent whole genome sequencing to determine MLST, phylogroups, resistance genes, virulence determinants and plasmid replicons. Results: ESBL prevalence was highest in human isolates (73%), followed by sewage (28.6%) and animals (16.3% diseased; 8% healthy). blaCTX-M-15 predominated in humans, whereas blaCTX-M-55 dominated in animals and sewage, suggesting ecological partitioning with partial overlap. Quinolone resistance was lowest in the animal interface. Sewage isolates harbored the most complex resistome, including rmtB and plasmid-mediated quinolone resistance genes. MLST analysis revealed high diversity in human isolates, including globally recognized ExPEC lineages (ST10, ST38, ST73, ST127, ST131), while ST224 dominated in animals with evidence of possible spillover to humans. ST167 was confined to sewage, consistent with environmental maintenance of high-risk clones. Phylogroup structuring showed predominance of A, B2 and D among human isolates and A, B1, and E among animal and sewage isolates. Virulence profiling demonstrated broader virulome diversity in humans, but shared core determinants (fimH, sitA, traT) across all domains. IncFIB(AP001918) was the dominant plasmid replicon, particularly among ESBL isolates, underscoring its role in horizontal gene dissemination. Alarmingly, mutation in pmrB (V161G) was identified in a healthy animal isolate, pointing to a need for greater colistin restriction in animal husbandry. Conclusions: This study highlights plasmid-mediated resistance and shared virulence determinants linking reservoirs; although AMR profile was quite distinct across the three interfaces, human isolates demonstrated greater resistance than animal isolates, suggesting healthcare-driven AMR in Oman. Continued integrated genomic surveillance is essential to monitor gene flow and inform coordinated antimicrobial stewardship strategies. Full article

Textile industry wastewater poses a significant environmental challenge due to the presence of persistent dyes. Cationic dyes are characterized by resistance to the conventional coagulation method. The appropriate properties and combination of chemicals guarantee an effective removal process. This study explains the effect of modification of methylene blue solution by the addition of a natural biopolymer—hydrolyzed tannic acid (TA). The study assumed that a combination of tannic acid, methylene blue and polyaluminum chloride would provide a synergistic effect and significantly improve the coagulation and sediment filtration process. Coagulation tests were carried out for a range of methylene blue concentrations. The optimal arrangement of solution components and coagulant doses was selected and tested. Over 95% dye removal efficiency was achieved. The maximum dye removal efficiency was determined to be 5 mg/mg Al at pH = 5.0. Based on the analysis of UV-VIS spectroscopy, FTIR and electrokinetic potential, changes in the solutions of tannin-modified dyes and their effect on the precipitation of flocs and the nature of sorption were determined. The main phenomena affecting the removal mechanism are discussed. The results indicate that tannic acid can serve as a sustainable coagulant aid, supporting the development of technologies for treating cationic-dye-laden wastewater. Full article

The accumulated ammonia within the recirculating aquaculture systems threaten fish health, while little is known about the influences during early fish ontogeny. Using larval and juvenile yellow catfish (Pelteobagrus fulvidraco) as a model, a comprehensive experiment exposing fish to varying total ammonia nitrogen concentrations (0, 10, 20 mg/L for larvae; 0, 25, 125 mg/L for juveniles) was conducted to evaluate the effects on gill transcriptome and microbiota along with the serotonergic regulation. First, the serotonin (5-HT) signal, which controls oxygen chemoreception and ventilation, was mainly detected in the surface of the body of the larvae, and then shifted to gill filaments of juveniles, showing a transition from cutaneous to branchial respiration. Both larval and juvenile yellow catfish exhibited reduced survival, damaged gill structure, and elevated 5-HT expression after ammonia exposure, as well as upregulated tph1b, slc6a4b, scgn and lama5 expression with the increased ammonia concentration, indicating the effects on respiratory function via serotonergic regulation. Further transcriptome analysis was conducted in juveniles to identify the differentially expressed genes (DEGs) and thus, to illustrate more detailed responses after ammonia exposure; KEGG enrichment analysis of DEGs indicated the coping strategy shifted from metabolic buffering to metabolic elimination via glutamine synthesis with the increased ammonia level. The qRT-PCR experiment also identified the increased expression of genes involved in the urea cycle—such as ass1, asl and glula—with the increased ammonia level. Considering the potential contributary role of microbiome to gill health, 16S sequencing was conducted on the gill in the control and the 125 mg/L ammonia-exposed group. Ammonia exposure at 125 mg/L induced significant variation in Simpson index and a marked decline in β diversity. Notably, the abundance of opportunistic pathogens such as Pseudomonadota increased, while the abundance of Deinococcota and Deinococcus—which were renowned for exceptional stress resistance capacity—decreased after ammonia exposure. Thus ammonia exposure disrupts the transcriptomic and microecological balance within gill mucosa, which may elevate the risk of pathogenic infection. Overall, our study provided the first evidence of serotonergic regulation on early fish respiration during ammonia exposure, and also offered new theoretical insights into the involvement of microorganisms in ammonia toxicity. Full article

Background: Colistin (Col) resistance and heteroresistance in extensively drug-resistant (XDR) Acinetobacter baumannii severely limit therapeutic options. We investigated the activity and mechanism of human albumin nanoparticles (haNPs) as colistin potentiators against genetically characterized clinical isolates. Methods: Sixteen clinical isolates were analyzed. Col MICs were determined by broth microdilution, and heteroresistance by population analysis profiling. Potentiation of Col activity was assessed using both Col-loaded haNPs (Col/haNPs) and free Col co-administered with empty haNPs, alongside the proton motive force (PMF) uncoupler carbonyl cyanide 3-chlorophenylhydrazone (CCCP). Assays included checkerboard synergy (FICI), membrane potential analysis (DiOC₂(3)), intracellular Col quantification (UPLC–MS/MS), zeta potential measurements, transmission electron microscopy (TEM), protein leakage, and ROS detection. Results: Heteroresistance was detected in 9/16 isolates. Col/haNPs reduced Col MICs by 4–64-fold in resistant strains and shifted MICs to ≤2 mg/L in most heteroresistant isolates. Empty haNPs displayed no intrinsic antibacterial activity yet selectively potentiated Col, with strong synergy (FICI down to 0.035). Membrane depolarization and increased intracellular Col accumulation under haNP-treated conditions paralleled the effects of CCCP, indicating that haNPs elicit a CCCP-like functional response. These findings are compatible with perturbation of membrane energetics and possible downstream effects on PMF-dependent transport processes. TEM and surface charge analyses supported direct nanoparticle–envelope interaction and progressive membrane disruption. Conclusions: haNPs enhance Col activity across genetically diverse A. baumannii isolates, with particularly strong effects in heteroresistant strains. The combined effects of PMF modulation, increased intracellular drug availability, and envelope interaction provide a mechanistic rationale for the use of albumin-based nanoparticles, either as Col carriers or in combination with free drug, to overcome Col resistance and heteroresistance. Full article

This article examines the early formation of Confucian–Islamic synthesis during the Yuan dynasty, arguing that institutional and intellectual adaptations in this period laid the groundwork for the later systematic synthesis known as “Yi-Ru Huitong” (伊儒會通). Moving beyond narratives of assimilation or resistance, it analyzes how Muslim communities navigated China’s pluralistic sociopolitical landscape through a process of creative adaptation. Employing a multidisciplinary approach that integrates textual analysis, historical comparison, and transcultural theory, the study investigates three key dimensions: the development of hybrid religious institutions, legal-political negotiations, and mechanisms of social integration. Drawing on multilingual sources—including Persian Islamic manuals, Yuan administrative archives, and epigraphic evidence—it demonstrates how Yuan-era Muslims established patterns of selective adaptation that preserved Islamic identity while enabling meaningful engagement with Chinese cultural norms. These developments not only ensured the survival of Islam in China but also generated a range of transcultural achievements in astronomy, medicine, architecture, and the literary arts, thereby creating the necessary conditions for the profound philosophical syntheses of the Ming-Qing era. By positioning the Yuan period as a crucial incubator of Sino-Islamic civilization, this study offers insights for comparative philosophy and the global history of civilizational dialog, inviting reflection on the early Chinese Islamic experience as a significant case of sustainable cross-civilizational engagement. Full article

Edible insects (EIs) have been suggested as a sustainable alternative to meat from traditional sources. However, despite EIs being consumed by humans since time immemorial, it is also true that, in many areas, especially in Western countries, there is some resistance and even neophobia to their acceptance as food. This work aimed to investigate the perceptions about EIs and validate a corresponding scale. This descriptive cross-sectional study was carried out by means of a questionnaire survey on a sample of 3711 participants in six countries (Croatia, Lebanon, Lithuania, Mexico, Portugal and Romania). Data were analysed by factor and cluster analyses, as well as other statistical tools. The results showed that, out of the initial 36 items in the questionnaire, 24 were validated in the scale of perceptions, distributed across six factors. Cluster analysis showed that the participants could be grouped according to three clusters (Traditionalists, Shoppers and Innovators). Statistical tests revealed significant differences (p < 0.05) between the clusters in practically all sociodemographic and consumption variables studied. Finally, a word analysis of the 2263 words indicated by the participants when thinking about EIs revealed that the most frequent words were ‘disgusting’, ‘protein’, and ‘nutritive’ in all three clusters. However, differences were observed after the fourth position in the list of most frequent words. In conclusion, these results indicate that perceptions of EIs were highly variable across countries of origin and other sociodemographic groups, and that, globally, participants exhibited some neophobia towards EIs. However, they recognised their value in terms of nutrition, particularly protein. This work evidences some relevant aspects that can be useful to adapt and define targeted policies to demystify the negative perceptions about EIs and improve consumer acceptance, thus contributing to more sustainable food systems. Full article

The cigarette beetle, Lasioderma serricorne, is a globally important pest of stored products, and prolonged fumigant use has accelerated resistance development. Glutathione S-transferases (GSTs) are key phase II detoxification enzymes that mediate insect tolerance to xenobiotics. In this study, we identified nine GST genes (LsGSTs) in L. serricorne and classified them into four cytosolic classes, namely epsilon, delta, theta, and sigma, based on phylogenetic analysis. Most LsGSTs were predominantly expressed during larval stages, while LsGSTs7 showed peak expression in adults. Tissue-specific profiling revealed predominant expression in metabolically active organs, including the fat body, Malpighian tubules, and midgut. Inhibition of GST activity using diethyl maleate (DEM) significantly increased larval susceptibility to three emerging fumigants: ethyl formate, benzothiazole, and methyl isothiocyanate. Exposure to LC₃₀ and LC₅₀ concentrations of these fumigants induced up-regulation of multiple LsGSTs, highlighting fumigant-specific detoxification responses. RNA interference targeting nine fumigant-inducible LsGSTs markedly elevated mortality and decreased total GST activity under fumigant stress. Furthermore, recombinant LsGSTs6 protein effectively metabolized methyl isothiocyanate, confirming their direct role in fumigant detoxification. Collectively, these findings provide novel insights into the molecular mechanisms underlying GST-mediated tolerance in L. serricorne and identify specific GST isoenzymes as promising molecular targets for innovative resistance management strategies in stored-product pest control. Full article

This study explored the eco-friendly synthesis of AgNPs using Prosopis laevigata seed mucilage and assessed their antimicrobial, antibiofilm, and biocompatibility effects against foodborne pathogens. The AgNPs were mostly spherical, with sizes ranging from 2.5 to 56 nm (average: 14.69 nm), as confirmed by XRD and DLS analysis. They showed consistent antimicrobial activity, with MICs at 0.5 mg/mL and MBCs at 1.0 mg/mL across all tested strains, and inhibited bacterial growth by over 75% at 0.5–5 mg/mL, similar to or better than gentamicin. The antibiofilm performance was notable, with inhibitions of 76–84% against E. coli (1–10 mg/mL), 96–98% against S. aureus (0.5–10 mg/mL), 76–82% against Salmonella Typhimurium (0.5–10 mg/mL), and 70–84% against P. aeruginosa (1–10 mg/mL), surpassing gentamicin against E. coli and P. aeruginosa. Cell viability remained 100% at 0.25 mg/mL, and no significant changes in immunological parameters were observed, suggesting good biocompatibility at therapeutic doses. This research shows, for the first time, that P. laevigata mucilage is an effective bioreducing agent for green synthesis of AgNPs with antimicrobial and antibiofilm activity against both Gram-negative and Gram-positive foodborne pathogens. Its superior ability to inhibit biofilms compared to traditional antibiotics, along with its safety profile at therapeutic levels, makes these nanoparticles promising for food safety applications, antimicrobial coatings, and topical treatments. Overall, the findings support the use of native plant resources in green nanotechnology to address global challenges of antimicrobial resistance. Full article

Baicalin, a natural plant-derived compound, holds promise in addressing clinical bacterial resistance when combined with antibiotics. This study evaluated the antibacterial activity of the combination of baicalin and cefotaxime and explored its mechanism of action on the cell wall and biofilm of multidrug-resistant Pseudomonas aeruginosa (MRPA). The results showed that the combination of baicalin and cefotaxime exerted a synergistic inhibitory effect on the growth of MRPA, with a fractional inhibitory concentration index (FICI) of 0.28. Mechanistically, compared with cefotaxime alone, the combination of baicalin and cefotaxime enhanced the permeability of the cell membrane and cell wall of MRPA, thereby increasing cell damage. It also exhibited stronger antibiofilm activity by inhibiting numerous virulence factors (pyocyanin, elastase, lectin), reducing cellular metabolic activity, and downregulating the expression of biofilm genes (pslA, pelA, algD) and quorum-sensing genes (lasl, lasR, rhll, rhlR, pqsA, pqsR). The molecular docking results revealed that baicalin could stably bind to wbpE, LasR, and RhlR. Therefore, this interaction may indirectly influence the processes related to antibiotic resistance and biofilm formation in bacterial cells. Metabolomic analysis revealed that the combination of baicalin and cefotaxime upregulated 863 metabolites and downregulated 587 metabolites. These metabolites mainly included amino acids, lipids, nucleotides, carbohydrates, and secondary metabolites. The combination primarily enriched key pathways such as amino acid metabolism, lipid metabolism (sphingolipid metabolism) and secondary metabolite biosynthesis. Through these pathways, it triggers significant metabolic reprogramming, thereby interfering with the supply of cell wall synthesis precursors, membrane structural stability, and the generation of biomembrane matrix. Ultimately, it synergistically enhances the effects of cell wall damage and biomembrane inhibition. In conclusion, this study confirms that the combination of baicalin and cefotaxime exerts significant synergistic antibacterial activity against MRPA. It also reveals the mechanism of action of the combination on the cell wall and biofilm of MRPA at the metabolic level, providing theoretical support for the development of novel strategies to combat MRPA. Full article

In 2023, the European Centre for Disease Prevention and Control surveillance report highlighted an increasing number of carbapenem-resistant Escherichia coli isolates carrying the less common bla_NDM-5 variant in Europe. The aim of this study was to investigate the molecular epidemiology of NDM-producing (New Delhi metallo-β-lactamase) E. coli isolates collected in Croatia over a one-year period. A total of 160 carbapenemase-producing E. coli isolates were reported through national surveillance in Croatia between March 2023 and March 2024. Whole-genome sequencing was performed on 22 NDM-producing E. coli isolates. Phylogenetic analysis identified 17 sequence types, indicating high diversity and polyclonal spread. High variability in resistome profiles and co-occurrence of resistance genes across multiple antimicrobial classes indicate multidrug resistance. The predominant bla_NDM variant was bla_NDM-1 (77.27%), followed by bla_NDM-5 (22.73%). Co-occurrence of bla_NDM with extended-spectrum β-lactamase (ESBL) encoding genes was detected in 12/22 isolates (54.55%). Plasmid analysis identified 22 different replicon types, with IncFII (54.54%) and IncA/C2 (45.45%) being the most frequent. Our findings provide insights into the molecular epidemiology of NDM-producing E. coli at the national level, highlighting the presence of the bla_NDM-5 variant. These results emphasize the need for genomic surveillance and strengthened infection control strategies to better understand and limit its spread. Full article

In vitro cell barrier models have been increasingly integrated into pharmaceutical and academic research pipelines to evaluate drug safety and drug delivery due to a shift towards New Approach Methodologies (NAMs) in research and regulatory safety assessment. Such models require reliable and interpretable functional readouts. Bioimpedance-based monitoring, particularly transepithelial/endothelial electrical resistance (TEER), is a widely adopted readout due to its non-invasive and real-time capabilities. However, substantial variability arises from differences in measurement settings, frequency selection, electrode configuration, impedance measuring techniques, and data analysis strategies. In numerous studies, TEER is approximated from single-frequency impedance magnitude measurements, which do not isolate the resistive component associated with tight junction-mediated paracellular transport but instead reflect the combined response of a coupled electrochemical system. This review clarifies impedance measuring techniques and systematically analyzes impedance-based measurement and analysis strategies for in vitro biological cell barrier integrity. We compare mono-frequency and broadband acquisition approaches, examine the influence of electrode–electrolyte interfaces, electrode geometry, and culture configuration, and evaluate equivalent circuit modeling and phase-resolved electrical impedance spectroscopy (EIS). Based on this comparison, we propose a three-level analytical hierarchy adapted to experimental objectives and instrumentation constraints. We conclude that phase-informed impedance analysis and harmonized reporting are essential to improve measurement reproducibility, inter-platform comparability, and integration of impedance-derived cell barrier assessment within NAMs-oriented research workflows. Full article

Aphanomyces root rot is a major threat to legume production worldwide, mainly in pea and lentil, crops on which extensive research programs are targeting the management of the disease. However, other legumes such as common vetch, although known to be severely affected by the disease, remain largely unexplored. This study aimed to identify sources of resistance within V. sativa subsp. sativa accessions. A total of 211 genetically diverse accessions were screened under controlled conditions following inoculation with isolate RB84. Disease progression was monitored through periodic foliar assessments and final root symptom evaluation. To assess resistance stability, a subset of 13 accessions representing contrasting response levels was further inoculated with three additional isolates (Aph-1, AE11, and AE12). In this multi-isolate assay, disease severity was quantified, shoot biomass was recorded, and root system architecture traits were determined using WinRHIZO image analysis. A high correlation between foliar and root symptoms at 20 days indicated that foliar symptom assessment provides a reliable, non-destructive indicator of root health. Considerable variation in disease response was detected, with several genotypes maintaining consistently low symptom levels and three exhibiting near-complete resistance across all isolates. Root architectural traits further corroborated visual disease assessments, showing patterns consistent with resistance and susceptibility responses. Overall, this study demonstrates the presence of genetic variability in the response of V. sativa to A. euteiches, with a subset of accessions showing resistance to the four isolates tested. This resistance potential can be directly used in breeding programs focused on improving tolerance to root rot. Full article

Powdery scab, caused by Spongospora subterranea, is a major disease of potato in which host resistance remains poorly understood at the biochemical level. While previous transcriptomic and proteomic studies have implicated glutathione S-transferases (GSTs) in cultivar-specific defence responses, orthogonal evidence at the metabolite level remains limited. In this study, untargeted metabolomics was applied to investigate root metabolic responses of two potato cultivars with contrasting resistance to S. subterranea. The relatively resistant cultivar ‘Gladiator’ and the susceptible cultivar ‘Iwa’ were inoculated with S. subterranea, and roots were collected at the stage of visible gall formation for analysis by high-resolution liquid chromatography–mass spectrometry (LC-MS/MS). Principal component analysis revealed a distinct metabolic profile in infected ‘Gladiator’ roots compared with both non-inoculated controls and infected ‘Iwa’ roots, indicating a stronger host metabolic response in the resistant cultivar. Among the annotated metabolites, cysteinyl-glycine (Cys-Gly), a central intermediate of glutathione turnover, was significantly more abundant in infected ‘Gladiator’ roots. The accumulation of Cys-Gly provides direct biochemical evidence linking enhanced glutathione cycling and GST activity to effective host defence. These findings highlight glutathione metabolism as a key component of potato resistance and demonstrate the value of metabolomics for additional validation of biochemical mechanisms underlying plant cultivar responses to biotic stress. Full article

Global environmental norms increasingly shape commodity governance in the Global South, with the EU Deforestation-Free Regulation (EUDR) representing a prominent attempt to govern land-based products through extraterritorial sustainability criteria. This study examines how such norms are received, reinterpreted, negotiated, and resisted in Indonesia’s palm oil sector, focusing on smallholder-dominated value chains in Serdang Bedagai and Simalungun, North Sumatra. Centered on everyday resistance and policy decoupling as its core interpretive lenses, and drawing on habitus as a supporting concept, the study employs qualitative fieldwork, in-depth interviews, field observations, and critical discourse analysis to investigate tensions between deforestation-free supply chain expectations and local realities marked by fragmented landholdings, informal tenure, intermediary dependence, and cashflow-oriented livelihood strategies. The findings show that the EUDR is widely perceived not as a sustainability opportunity, but as an externally imposed regulatory pressure that threatens income stability and market access. Local actors respond through discursive reframing, continued reliance on informal trading practices, and partial or symbolic implementation of legality and traceability requirements. The study argues that inclusive deforestation-free governance requires differentiated obligations, transitional legality pathways, and cooperative-based traceability mechanisms that better align global norms with local institutional capacity and livelihood structures. Full article

Trichophyton indotineae is an emerging dermatophyte associated with extensive, chronic, recalcitrant, and frequently terbinafine-resistant dermatophytosis worldwide. In this study, 30 T. indotineae strains isolated in Italy were investigated. The isolates were obtained from patients originating from Asian countries, from patients from other countries, and from Italian patients who reported no travel outside Italy in the preceding years. Clinical isolates were identified by internal transcribed spacer (ITS) sequencing and analyzed to assess the occurrence and molecular basis of terbinafine resistance. Terbinafine resistance was detected in 18 strains (60%) using a real-time PCR assay. Sequencing of the squalene epoxidase (SQLE) gene revealed mutations associated with resistance, including L393S in nine strains and F397L in another nine strains. NGS analysis confirmed two terbinafine-resistant strains carrying the L393S and F397L mutations, respectively, and detected the A448T mutation in one terbinafine-susceptible strain. These findings demonstrate the presence of terbinafine-resistant T. indotineae across five regions of Italy and confirm the occurrence of SQLE mutations previously linked to antifungal resistance. Data obtained also support a link with endemic Asian areas, other than suggesting the possible occurrence of autochthonous transmission in Italy. Full article