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Keywords = repeated tightening

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12 pages, 5507 KB  
Article
Effects of Abutment Screw Preload on Peri-Implant Bone Tissue Under Dynamic Loading: A Preliminary In Vivo Rabbit Study
by Yu Yamamoto, Masako Nagasawa, Tongtong Zhang, Kosuke Nozaki and Katsumi Uoshima
Appl. Sci. 2026, 16(11), 5227; https://doi.org/10.3390/app16115227 - 23 May 2026
Viewed by 288
Abstract
This study evaluated how abutment screw preload affects peri-implant bone under vertical dynamic loading using an in vivo rabbit tibia model. Eight Japanese white rabbits received two implants in each tibia. After 8 weeks of healing, implants were assigned to a control group [...] Read more.
This study evaluated how abutment screw preload affects peri-implant bone under vertical dynamic loading using an in vivo rabbit tibia model. Eight Japanese white rabbits received two implants in each tibia. After 8 weeks of healing, implants were assigned to a control group without abutment connection or to abutment-connected groups tightened to 35 Ncm or 70 Ncm. The abutment groups were further divided into loading and non-loading subgroups. In the loading groups, vertical dynamic loading (50 N, 3 Hz, 1800 cycles) was applied twice weekly for 4 weeks. Peri-implant bone responses were assessed by micro-computed tomography, histology, and histomorphometry. Under loading conditions, the 35 Ncm group showed significantly higher bone volume, bone-to-implant contact, and bone area fraction than the 70 Ncm group (p < 0.05). Histologically, the 35 Ncm group exhibited more continuous cortical bone and new bone formation, whereas the 70 Ncm group more frequently showed cortical discontinuity and enlarged marrow spaces. Within the limitations of this animal study, abutment screw preload influenced peri-implant bone adaptation under repeated loading, and the manufacturer-recommended torque of 35 Ncm was associated with more favorable bone parameters than the 70 Ncm condition. Full article
(This article belongs to the Section Applied Dentistry and Oral Sciences)
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27 pages, 12622 KB  
Article
Safety-Filtered Residual Reinforcement Learning over Model Predictive Control for Friction-Aware Autonomous Vehicle Platooning
by Ali S. Allahloh, Atef M. Ghaleb, Mohammad Sarfraz, Abdalla Alrashdan, Mohammed A. H. Ali and Adel Al-Shayea
Machines 2026, 14(5), 560; https://doi.org/10.3390/machines14050560 - 16 May 2026
Viewed by 358
Abstract
This paper presents a deployment-oriented longitudinal platoon-control architecture for connected and autonomous vehicles operating under repeated leader hard-braking, cut-ins, and spatially varying road friction. The proposed stack combines four elements: (i) a lightweight scalar Kalman filter (KF) that smooths a friction-related signal and [...] Read more.
This paper presents a deployment-oriented longitudinal platoon-control architecture for connected and autonomous vehicles operating under repeated leader hard-braking, cut-ins, and spatially varying road friction. The proposed stack combines four elements: (i) a lightweight scalar Kalman filter (KF) that smooths a friction-related signal and feeds friction-dependent constraint tightening; (ii) a model predictive control (MPC) backbone whose weights and horizon are selected offline using multi-objective GA/NSGA-II tuning; (iii) a bounded proximal policy optimization (PPO) residual policy, trained with the aid of a learned surrogate model, that refines the MPC command during transient events; and (iv) a command-level safety projection that enforces instantaneous actuation and clearance constraints at the fast control tick. The contribution is therefore not a new MPC formulation or a new reinforcement-learning algorithm in isolation, but an integrated and experimentally characterized control stack that keeps the safety-critical structure explicit while using learning to improve transient behavior. The method is evaluated in a CARLA digital twin of a six-vehicle platoon over a 5 km mixed urban–highway route and is further assessed in hardware-in-the-loop (HIL) on an automotive ECU using a multi-rate ROS 2/AUTOSAR implementation (50 Hz estimation/safety loop, 10 Hz MPC/RL refresh). Across 10 held-out disturbance seeds, the full stack improves spacing regulation, maintains non-amplifying disturbance propagation according to the reported string-stability indices, and reduces a route-normalized positive tractive-energy-at-the-wheels proxy by about 12% relative to Manual MPC and by up to 18% relative to a PID-CACC reference. Because the PID-CACC baseline does not enforce hard constraints and can collide under the tested disturbance suite, the main performance comparison is among collision-free controllers. The friction signal used in CARLA is derived from simulator road-surface annotations before filtering, so the present study should be interpreted as a friction-aware control and integration study rather than a validated onboard friction-estimation result. Likewise, the reported energy metric is an effort proxy and is not a calibrated fuel or battery consumption model. Full article
(This article belongs to the Special Issue Reinforcement Learning for Autonomous Vehicle Control)
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25 pages, 3649 KB  
Article
Comparative Analysis of CFD Simulations and Empirical Studies for a Heat Exchanger in a Dishwasher
by Wojciech Skarka, Maciej Mazur, Damian Kądzielawa and Robert Kubica
Energies 2025, 18(24), 6609; https://doi.org/10.3390/en18246609 - 18 Dec 2025
Cited by 1 | Viewed by 1111
Abstract
This paper presents a side-by-side study of CFD predictions and experimental measurements for a novel counter-flow heat exchanger installed in the sidewall of a dishwasher (HEBS). The work aims to improve appliance efficiency by transferring heat from discharged hot wastewater to the incoming [...] Read more.
This paper presents a side-by-side study of CFD predictions and experimental measurements for a novel counter-flow heat exchanger installed in the sidewall of a dishwasher (HEBS). The work aims to improve appliance efficiency by transferring heat from discharged hot wastewater to the incoming cold supply. Motivated by sustainability goals and tightening EU energy rules, the research targets the high losses typical of conventional machines. This approach combines detailed ANSYS Fluent 2022R2 simulations with controlled laboratory tests on a bespoke test rig. The measured data show a repeatable rise in the cold-water temperature of roughly 8 K, corresponding to an approximate 15% gain in thermal performance for the heat-recovery stage. While the simulations and experiments efficiently agree based on trends and qualitative behavior, there are noticeable quantitative differences in the total energy transfer, indicating the models need further refinement. The validation carried out here forms a solid basis for design optimization and for reducing energy consumption in household dishwashers. This work overcomes the limitations of previous studies which typically rely on external storage tanks or static heat recovery analysis. The primary novelty of this paper lies in the empirical validation of a high-efficiency heat exchanger integrated into the extremely constrained sidewall volume of the appliance, tested under transient, on-the-fly flow conditions, providing a verified methodology for constrained industrial applications. Full article
(This article belongs to the Special Issue Computational Fluid Dynamics (CFD) Study for Heat Transfer)
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10 pages, 784 KB  
Article
The Acute Effects of Internal, External, and Golf-Specific Attentional Focus Cues on Isometric Trunk Strength in Youth Golfers
by Raouf Hammami, Achraf Hammami, Yassine Negra, Rimeh Staff, Jason Moran and Roland van den Tillaar
J. Funct. Morphol. Kinesiol. 2025, 10(4), 435; https://doi.org/10.3390/jfmk10040435 - 7 Nov 2025
Viewed by 1564
Abstract
Background: Attentional focus strategies, including internal, external, and sport-specific cues, can influence muscle strength by modulating motor control. However, their acute effects on maximal isometric back-extensor strength in youth athletes under controlled laboratory conditions remain unclear. Methods: Fourteen youth golfers (15.8 [...] Read more.
Background: Attentional focus strategies, including internal, external, and sport-specific cues, can influence muscle strength by modulating motor control. However, their acute effects on maximal isometric back-extensor strength in youth athletes under controlled laboratory conditions remain unclear. Methods: Fourteen youth golfers (15.8 ± 0.5 years) performed maximal voluntary isometric back-extension tasks under nine cueing conditions: three internal, three external, and three golf-specific. The task involved exerting maximal force against a fixed, immovable resistance while maintaining standardized trunk and hip positions to ensure consistent execution. Cueing was delivered verbally in a standardized manner across participants and sessions. Maximal isometric strength was compared across conditions using repeated-measures analyses. Results: Maximal isometric back-extensor strength was significantly (p = 0.004 ηp2 = 0.34) lower with internal cues (57.1 ± 16.0 kg) compared with external (68.2 ± 13.0 kg) and golf-specific (68.1 ± 12.5 kg) cues. Specifically, the internal cues ‘engage your glutes and hamstrings’, ‘tighten your core’, and ‘maintain a neutral spine’ produced lower force than all external cues and the golf-specific cue ‘focus on using your lower body to create a stable base for your golf swing’. Among internal cues, ‘engage your glutes and hamstrings’ resulted in the lowest torque. Conclusions: External and certain golf-specific verbal cues acutely enhance maximal isometric back-extensor force more effectively than internal cues in a controlled laboratory setting. While these results inform how attentional focus can modulate acute force output in youth athletes, the task does not replicate the dynamic, rotational nature of the golf swing, and the findings should not be interpreted as direct indicators of golf performance. Future research should explore long-term adaptations and assess transfer to sport-specific, dynamic movements. Full article
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18 pages, 4143 KB  
Article
Binocular Stereo Vision-Based Structured Light Scanning System Calibration and Workpiece Surface Measurement Accuracy Analysis
by Xinbo Zhang, Li Luo, Rui Ma, Yuexue Wang, Shi Xie, Hao Zhang, Yiqing Zou, Xiaohao Wang and Xinghui Li
Sensors 2025, 25(20), 6455; https://doi.org/10.3390/s25206455 - 18 Oct 2025
Cited by 1 | Viewed by 1633
Abstract
Precise online measurement of large structural components is urgently needed in modern manufacturing and intelligent construction, requiring a measurement range over 1 m, near-millimeter accuracy, second-level measurement speed, and adaptability to complex environments. In this paper, three mainstream measurement technologies, namely the image [...] Read more.
Precise online measurement of large structural components is urgently needed in modern manufacturing and intelligent construction, requiring a measurement range over 1 m, near-millimeter accuracy, second-level measurement speed, and adaptability to complex environments. In this paper, three mainstream measurement technologies, namely the image method, line laser scanning method, and structured light method, are comparatively analyzed. The structured light method exhibits remarkable comprehensive advantages in terms of accuracy and speed; however, it suffers from the issue of occlusion during contour measurement. To tackle this problem, multi-camera stitching is employed, wherein the accuracy of camera calibration plays a crucial role in determining the quality of point cloud stitching. Focusing on the cable tightening scenario of meter-diameter cables in cable-stayed bridges, this study develops a contour measurement system based on the collaboration of multiple structured light cameras. Measurement indicators are optimized through modeling analysis, system construction, and performance verification. During verification, four structured light scanners were adopted, and measurements were repeated 11 times for the test workpieces. Experimental results demonstrate that although the current measurement errors have not yet been stably controlled within the millimeter level, this research provides technical exploration and practical experience for high-precision measurement in the field of intelligent construction, thus laying a solid foundation for subsequent accuracy improvement. Full article
(This article belongs to the Section Sensing and Imaging)
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14 pages, 2459 KB  
Article
Investigating the Correlation Between Corrosion-Induced Bolt Head Damage and Preload Loss Using Ultrasonic Testing
by Jay Shah, Hao Wang and Abhijit Mukherjee
Sensors 2025, 25(14), 4491; https://doi.org/10.3390/s25144491 - 19 Jul 2025
Cited by 4 | Viewed by 1113
Abstract
The integrity of bolted components primarily relies on the quality of interfacial contact, which is achieved by maintaining prescribed bolt torque levels. However, challenges arise from corrosion-induced bolt head damage, potentially compromising the bolt preload, and quantifying such effects remains unanswered. Many studies [...] Read more.
The integrity of bolted components primarily relies on the quality of interfacial contact, which is achieved by maintaining prescribed bolt torque levels. However, challenges arise from corrosion-induced bolt head damage, potentially compromising the bolt preload, and quantifying such effects remains unanswered. Many studies often compare bolt corrosion’s effects to bolt loosening as both affect the interfacial contact stresses to some extent. This technical study aimed to investigate whether a correlation exists between the impact of bolt head damage and the different levels of bolt torque. Guided wave ultrasonic testing (UT) was implemented for this investigation. Laboratory experiments were conducted to monitor the transmission of ultrasonic signals across the bolted interface first during the bolt-tightening process. Once the highest bolt torque was achieved, the process was repeated for a simplified corrosion scenario, simulated by artificially damaging the bolt head in a controlled manner. The analysis focused on studying the transmission of signal energy for both scenarios. The findings revealed different trends for the signal energy transmission during bolt tightening, which are subjective to the inspection frequency. On the contrary, even at an advanced level of bolt head damage corresponding to 16% mass loss, no clear or monotonic trend was observed in the total transmitted energy. While the total energy remained relatively stable across all inspection frequencies, distinct waveform changes, such as energy redistribution and the emergence of additional wave packets, were observed. The findings emphasize the need for more advanced waveform-based analysis techniques to detect and interpret subtle changes caused by bolt degradation. Full article
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13 pages, 3211 KB  
Article
Dependence of the Preload on the Tightening Torque for Hydraulic Plugs
by Jurij Hladnik, Franc Majdič, Anže Čelik and Boris Jerman
Appl. Sci. 2024, 14(24), 11920; https://doi.org/10.3390/app142411920 - 19 Dec 2024
Cited by 3 | Viewed by 2234
Abstract
In hydraulics, threaded plugs are used to close various manufacturing holes and other fluid channels. They are preloaded to ensure sufficient sealing force. Since the range of recommended thread and underhead friction coefficients for preloaded threaded connections in the literature is very wide, [...] Read more.
In hydraulics, threaded plugs are used to close various manufacturing holes and other fluid channels. They are preloaded to ensure sufficient sealing force. Since the range of recommended thread and underhead friction coefficients for preloaded threaded connections in the literature is very wide, they are not suitable for accurate determination of the preload–torque relationships of plug–valve connections. In the study, two non-standard plugs with metric threads were equipped with strain gauges and repeatedly tightened three times in valve housings under lubricated and unlubricated conditions. The preload and tightening torque were measured. (1) Although the plug–valve connections had a similar geometry with the same surface roughness of the contacting surfaces, the average overall friction coefficient (uniform thread and underhead friction coefficient) and torque coefficient differed between the two connections in the unlubricated and lubricated conditions by 16% and 18%, respectively. This indicates that even small geometrical differences can have a considerable influence on these coefficients. The overall friction and torque coefficients were between 8% and 17% higher in the unlubricated condition than in the lubricated condition (not statistically proven). (2) The overall friction and torque coefficients decreased with repeated tightening under lubricated conditions. This influence decreased with the number of tightening repetitions. (3) Consideration of the minimum and maximum thread and underhead friction coefficients given in VDI 2230 would lead to an error in the estimated preload of −15% to +86%. In conclusion, for accurate determination of the preload–torque relationship of the plug–valve connections, measurements considering repeated tightening are crucial. These should be performed for each type and size of plug–valve connection separately. To minimize the repeated tightening influence, it is recommended to re-tighten the connections several times before leaving production. Full article
(This article belongs to the Special Issue Research Progress on Hydraulic Fluid and Hydraulic Systems)
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33 pages, 26346 KB  
Article
Horizontal Test Stand for Bone Screw Insertion
by Jack Wilkie, Georg Rauter and Knut Möller
Hardware 2024, 2(3), 223-255; https://doi.org/10.3390/hardware2030011 - 9 Sep 2024
Cited by 1 | Viewed by 2513
Abstract
Screws are a versatile method of fixation and are often used in orthopaedic surgery. Various specialised geometries are often used for bone screws to optimise their fixation strengths in limited spaces at the expense of manufacturing costs. Additionally, ongoing research is looking to [...] Read more.
Screws are a versatile method of fixation and are often used in orthopaedic surgery. Various specialised geometries are often used for bone screws to optimise their fixation strengths in limited spaces at the expense of manufacturing costs. Additionally, ongoing research is looking to develop systems/models to automatically optimise bone screw tightening torques. For both applications, it is desirable to have a test rig for inserting screws in a regulated, instrumented, and repeatable manner. This work presents such a test rig primarily used for the validation of optimal torque models; however, other applications like the above are easily foreseeable. Key features include controllable insertion velocity profiles, and a high rate measurement of screw torque, angular displacement, and linear displacement. The test rig is constructed from mostly inexpensive components, with the primary costs being the rotational torque sensor (approx. 2000 €), and the remainder being approximately 1000 €. This is in comparison to a biaxial universal testing machine which may exceed 100,000 €. Additionally, the firmware and interface software are designed to be easily extendable. The angular velocity profiling and linear measurement repeatability of the test rig is tested and the torque readings are compared to an off-the-shelf static torque sensor. Full article
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22 pages, 10563 KB  
Article
Low-Cost Cable-Driven Robot Arm with Low-Inertia Movement and Long-Term Cable Durability
by Van Pho Nguyen, Wai Tuck Chow, Sunil Bohra Dhyan, Bohan Zhang, Boon Siew Han and Hong Yee Alvin Wong
Robotics 2024, 13(9), 128; https://doi.org/10.3390/robotics13090128 - 27 Aug 2024
Cited by 13 | Viewed by 16344
Abstract
Our study presents a novel design for a cable-driven robotic arm, emphasizing low cost, low inertia movement, and long-term cable durability. The robotic arm shares similar specifications with the UR5 robotic arm, featuring a total of six degrees of freedom (DOF) distributed in [...] Read more.
Our study presents a novel design for a cable-driven robotic arm, emphasizing low cost, low inertia movement, and long-term cable durability. The robotic arm shares similar specifications with the UR5 robotic arm, featuring a total of six degrees of freedom (DOF) distributed in a 1:1:1:3 ratio at the arm base, shoulder, elbow, and wrist, respectively. The three DOF at the wrist joints are driven by a cable system, with heavy motors relocated from the end-effector to the shoulder base. This repositioning results in a lighter cable-actuated wrist (weighing 0.8 kg), which enhances safety during human interaction and reduces the torque requirements for the elbow and shoulder motors. Consequently, the overall cost and weight of the robotic arm are reduced, achieving a payload-to-body weight ratio of 5:8.4 kg. To ensure good positional repeatability, the shoulder and elbow joints, which influence longer moment arms, are designed with a direct-drive structure. To evaluate the design’s performance, tests were conducted on loading capability, cable durability, position repeatability, and manipulation. The tests demonstrated that the arm could manipulate a 5 kg payload with a positional repeatability error of less than 0.1 mm. Additionally, a novel cable tightener design was introduced, which served dual functions: conveniently tightening the cable and reducing the high-stress concentration near the cable locking end to minimize cable loosening. When subjected to an initial cable tension of 100 kg, this design retained approximately 80% of the load after 10 years at a room temperature of 24 °C. Full article
(This article belongs to the Section Industrial Robots and Automation)
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10 pages, 6640 KB  
Article
Effects of Bias Voltage and Target Current on Microstructure and Load Measurement Performance of ZnO Piezoelectric Coatings Applied to Bolt in Transformer
by Hanpeng Kou, Fuyuan Wang, Dayu Nie, Zhaojun Ning, Qiaoqiao Li, Jiangang Deng, Zhenbo Lan and Zhuolin Xu
Coatings 2023, 13(10), 1662; https://doi.org/10.3390/coatings13101662 - 22 Sep 2023
Viewed by 1587
Abstract
Electrical accidents caused by bolt looseness in transformers have been frequently reported in recent years. The monitoring, and warning of, axial force as an indicator of looseness is one of the key issues affecting the operation and maintenance of transformers. Traditional ultrasonic testing [...] Read more.
Electrical accidents caused by bolt looseness in transformers have been frequently reported in recent years. The monitoring, and warning of, axial force as an indicator of looseness is one of the key issues affecting the operation and maintenance of transformers. Traditional ultrasonic testing and a patch-type ultrasonic method, using piezoelectric probes and coupling agents, showed poor repeatability and accuracy in detecting the bolt pre-tightening force, because of the uncertainty of the contact interface produced via manual operation. A permanent thin-film pressure sensor (PMTS), which provides accurate and in-situ stress detection, is more suitable for bolts, to reveal the pretightening force. The key is depositing a nano-zinc oxide (ZnO) piezoelectric film with an excellent measurement performance, which could be tuned using deposition parameters. This paper investigates the effects of the current and bias voltage on the crystal structure and performance of ZnO piezoelectric films. The results show that the crystallinity degree and resistance decrease with the increase in bias voltage, while the target current could increase the crystallinity. However, a high current also brings large particles in the coating surface, which greatly decrease the resistance. The cause is expected to be related to the ion energy, which could be affected by the bias voltage and current. The PMTS deposited with an optimized bias voltage and current revealed excellent measurement performance, and is expected to be applied to the bolt, to detect the pretightening force. Full article
(This article belongs to the Special Issue Surface Coatings and Technology Against Soil Abrasion and Adhesion)
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15 pages, 5035 KB  
Article
Evaluation of Novel Embroidered Textile-Electrodes Made from Hybrid Polyamide Conductive Threads for Surface EMG Sensing
by Bulcha Belay Etana, Benny Malengier, Timothy Kwa, Janarthanan Krishnamoorthy and Lieva Van Langenhove
Sensors 2023, 23(9), 4397; https://doi.org/10.3390/s23094397 - 29 Apr 2023
Cited by 17 | Viewed by 4349
Abstract
Recently, there has been an increase in the number of reports on textile-based dry electrodes that can detect biopotentials without the need for electrolytic gels. However, these textile electrodes have a higher electrode skin interface impedance due to the improper contact between the [...] Read more.
Recently, there has been an increase in the number of reports on textile-based dry electrodes that can detect biopotentials without the need for electrolytic gels. However, these textile electrodes have a higher electrode skin interface impedance due to the improper contact between the skin and the electrode, diminishing the reliability and repeatability of the sensor. To facilitate improved skin–electrode contact, the effects of load and holding contact pressure were monitored for an embroidered textile electrode composed of multifilament hybrid thread for its application as a surface electromyography (sEMG) sensor. The effect of the textile’s inter-electrode distance and double layering of embroidery that increases the density of the conductive threads were studied. Electrodes embroidered onto an elastic strap were wrapped around the forearm with a hook and loop fastener and tested for their performance. Time domain features such as the Root Mean Square (RMS), Average Rectified Value (ARV), and Signal to Noise Ratio (SNR) were quantitatively monitored in relation to the contact pressure and load. Experiments were performed in triplicates, and the sEMG signal characteristics were observed for various loads (0, 2, 4, and 6 kg) and holding contact pressures (5, 10, and 20 mmHg). sEMG signals recorded with textile electrodes were comparable in amplitude to those recorded using typical Ag/AgCl electrodes (28.45 dB recorded), while the signal-to-noise ratios were, 11.77, 19.60, 19.91, and 20.93 dB for the different loads, and 21.33, 23.34, and 17.45 dB for different holding pressures. The signal quality increased as the elastic strap was tightened further, but a pressure higher than 20 mmHg is not recommended because of the discomfort experienced by the subjects during data collection. Full article
(This article belongs to the Special Issue Textile Sensors and Related Applications)
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19 pages, 29714 KB  
Article
Experimental Study on the Acoustic Emission Characteristics of Fractured Granite after Repeated High Temperature-Water Cooling
by Dong Zhu, Yuqing Fan, Yang Bai, Xiangling Tao, Leigang Miao and Huiwu Jin
Processes 2023, 11(1), 139; https://doi.org/10.3390/pr11010139 - 3 Jan 2023
Cited by 2 | Viewed by 2333
Abstract
Using the MTS816 rock mechanics servo tester, an acoustic emission monitoring system and high-speed digital photographic equipment, uniaxial compression tests were conducted on granite specimens containing single fracture slabs after repeated treatment (treatment times 1, 5, 10, 15 and 20) with three types [...] Read more.
Using the MTS816 rock mechanics servo tester, an acoustic emission monitoring system and high-speed digital photographic equipment, uniaxial compression tests were conducted on granite specimens containing single fracture slabs after repeated treatment (treatment times 1, 5, 10, 15 and 20) with three types of high temperature (250, 350 and 450 °C) water cooling, respectively, to analyze the basic mechanical parameters, acoustic emission change characteristics and fracture evolution of the specimens during the uniaxial compression process. It is shown that the heating temperature and the number of treatments not only have a deteriorating effect on the basic mechanical parameters of the specimens but also have an important effect on the changes in the basic parameters of acoustic emission at different compression stages. At 250 °C, the acoustic emission characteristics of the specimens at the initial tightening stage tended to decrease (N = 1 and 5 times) then, increase (N = 10 and 15 times) and then decrease (N = 20 times) as the number of treatments increased. At the same set temperature, the percentage of the bottom amplitude value of the acoustic emission of the specimen gradually decreases, and the percentage of the high amplitude value gradually increases as the number of treatments increases. After the specimen undergoes one and five treatments at 250 °C, the maximum acoustic emission energy value changes less, the maximum acoustic emission energy value decreases with the increase of treatment times in an approximately exponential function, the specimen is transformed from the brittle damage mode to the plastic damage mode and the effect of the prefabricated fracture on the damage of the specimen gradually disappears. Full article
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18 pages, 11241 KB  
Article
Molecular Modeling of Cardiac Sodium Channel with Mexiletine
by Boris S. Zhorov
Membranes 2022, 12(12), 1252; https://doi.org/10.3390/membranes12121252 - 10 Dec 2022
Cited by 4 | Viewed by 2444
Abstract
A sodium channel blocker mexiletine (MEX) is used to treat chronic pain, myotonia and some arrhythmias. Mutations in the pore domain (PD) of voltage-gated sodium channels differently affect tonic block (TB) and use-dependent block (UDB) by MEX. Previous studies identified several MEX-sensing residues [...] Read more.
A sodium channel blocker mexiletine (MEX) is used to treat chronic pain, myotonia and some arrhythmias. Mutations in the pore domain (PD) of voltage-gated sodium channels differently affect tonic block (TB) and use-dependent block (UDB) by MEX. Previous studies identified several MEX-sensing residues in the hNav1.5 channel and demonstrated that the channel block by MEX increases with activation of the voltage-sensing domain III (VSDIII), whereas MEX stabilizes the activated state of VSDIII. Structural rationales for these observations are unclear. Here, Monte Carlo (MC) energy minimizations were used to dock MEX and its more potent analog, Thio-Me2, into the hNav1.5 cryo-EM structure with activated VSDs and presumably inactivated PD. Computations yielded two ensembles of ligand binding poses in close contacts with known MEX-sensing residues in helices S6III, S6IV and P1IV. In both ensembles, the ligand NH3 group approached the cation-attractive site between backbone carbonyls at the outer-pore bottom, while the aromatic ring protruded ether into the inner pore (putative UDB pose) or into the III/IV fenestration (putative TB pose). In silico deactivation of VSDIII shifted helices S4–S5III, S5III, S6III and S6IV and tightened the TB site. In a model with activated VSDIII and three resting VSDs, MC-minimized energy profile of MEX pulled from the TB site towards lipids shows a deep local minimum due to interactions with 11 residues in S5III, P1III, S6III and S6IV. The minimum may correspond to an interim binding site for MEX in the hydrophobic path to the TB site along the lipid-exposed sides of repeats III and IV where 15 polar and aromatic residues would attract cationic blockers. The study explains numerous experimental data and suggests the mechanism of allosteric modification of the MEX binding site by VSDIII. Full article
(This article belongs to the Special Issue Biological Membrane and Bioactive Compounds Interactions)
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9 pages, 2642 KB  
Article
Assessment of Preload Loss after Cyclic Loading in the OT Bridge System in an “All-on-Four” Rehabilitation Model in the Absence of One and Two Prosthesis Screws
by Mario Cesare Pozzan, Francesco Grande, Edoardo Mochi Zamperoli, Fabio Tesini, Massimo Carossa and Santo Catapano
Materials 2022, 15(4), 1582; https://doi.org/10.3390/ma15041582 - 20 Feb 2022
Cited by 26 | Viewed by 3358
Abstract
The aim of this study was to evaluate the stability of prosthetic screws after applying cyclic loadings in an “all-on-four” rehabilitation model with the OT Bridge system. The model was tested both with and without anterior screws. Four implant analogues following the “all-on-four” [...] Read more.
The aim of this study was to evaluate the stability of prosthetic screws after applying cyclic loadings in an “all-on-four” rehabilitation model with the OT Bridge system. The model was tested both with and without anterior screws. Four implant analogues following the “all-on-four” concept were inserted in an edentulous mandibular resin model. An OT Bridge system with a Cr–Co prosthetic framework was fabricated. Depending on the presence or absence of one or two anterior screws on the implant analogues, three groups were created, i.e., Gr.1: three tightening screws, Gr.2: two tightening screws, Control Group: four tightening screws. Each single group underwent subsequent 400,000 cyclic loads, simulating approximately a year of chewing by using a dynamometer machine. This cycle was repeated five times for each group, and preload loss values were evaluated on each prosthetic screw after each cycle. All the data obtained were analyzed by one-way ANOVA and Student’s t-test. No statistically significant differences after intragroup analysis were found. A statistically significant difference within the Gr.1 between the screws in positions 33 and 36, equal to 15.2% (p-value = 0.0176), was found. The OT Bridge seems a useful system to maintain the retention of a prosthesis during mechanical stress conditions even in the absence of one screw in an “all-on-four” rehabilitation. This could represent a good solution to solve the esthetic problem of the screw buccal access hole for fixed solutions. Full article
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12 pages, 3842 KB  
Article
In Vitro Study of Preload Loss in Different Implant Abutment Connection Designs
by Ana Sofia Vinhas, Carlos Aroso, Filomena Salazar, Marta Relvas, Ana Cristina Braga, Blanca Ríos-Carrasco, Javier Gil, José Vicente Rios-Santos, Ana Fernández-Palacín and Mariano Herrero-Climent
Materials 2022, 15(4), 1392; https://doi.org/10.3390/ma15041392 - 14 Feb 2022
Cited by 22 | Viewed by 5086
Abstract
The stability and integrity of the abutment-implant connection, by means of a screw, is fallible from the moment the prosthetic elements are joined and is dependent on the applied preload, wear of the components and function. One of the main causes of screw [...] Read more.
The stability and integrity of the abutment-implant connection, by means of a screw, is fallible from the moment the prosthetic elements are joined and is dependent on the applied preload, wear of the components and function. One of the main causes of screw loosening is the loss of preload. The loosening of the screw-abutment can cause complications such as screw fracture, marginal gap, peri-implantitis, bacterial microleakage, loosening of the crown and discomfort of the patient. It is also reported that loosening of the screw/abutment may lead to a failure of osseointegration. It is necessary to evaluate and quantify, with in vitro studies, the torque loss before and after loading in the different connections. Aim: evaluate the influence of implant- abutment connection design in torque maintenance after single tightening, multiple tightening and multiple tightening followed by mechanical cycling. Materials and Methods: 180 Klockner implants divided in 4 groups: 15 SK2 external connection, 25 Ncm tightening torque; 15 KL external connection, 30 Ncm tightening torque; 15 Vega internal connection, 25 Ncm tightening torque; 15 Essential internal connection, 30 Ncm tightening torque. In each group removal torque values (RTV) were evaluated with a digital torque meter, in 3 distinct phases: after one single tightening, 10 multiple tightenings and 10 multiple tightenings and cyclic loading (500 N × 1000 cycles). Results: After one single tightening, and for all connections, RTV were lower than those of insertion, but only for Essential and Vega internal connections this result was statistically significant. After multiple tightening, RTV were significantly lower in all connections. After repeated tightening followed by cyclic loading, mean RTV were significantly lower, when compared to insertion torque. The multiple tightening technique resulted in higher RTV than the single tightening technique, except for Vega implant. The multiple tightening followed by cyclic load, compared to the other phases, was the one that generated the lowest RTV, for all connections. Conclusions: The connection design, in our study, did not seem to influence the maintenance of preload. Loading influenced the loss of preload, in the sense that significantly decreased the removal torque values. The multiple re-tightening technique resulted in higher removal torque values than the single tightening technique. Clinically, our results recommend to retighten retaining screws, a few minutes after insertion. Full article
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