Search Results (944)

Introduction: Botulinum toxin-A (BTX-A) injections are regularly used to treat temporomandibular disorders (TMD). However, consensus regarding the long-term efficacy of BTX-A for TMD-related myalgia remains lacking. This pragmatic, practice-based clinical study aimed to evaluate the Patient-Reported Outcome Measures of pain, health status, quality of life, and function after BTX-A injections in patients with TMD-related myalgia. Methods: This prospective cohort study included 35 patients with TMD-related myalgia who received BTX-A injections in the masseter and temporalis muscles. The Visual Analogue Scale for pain, the EQ-5D-3L for health status, the Oral Health Impact Profile-14 for oral health-related quality of life, the Mandibular Function Impairment Questionnaire for function and the maximum interincisal opening were assessed before treatment and at one, three and six months follow-up. Results: Patients reported a statistically significant and clinically relevant reduction in pain (p < 0.001), improvement of health status (p ≤ 0.003), and oral health-related quality of life (p < 0.001) at one-month follow-up, which remained present at three and six months post-treatment. Self-reported mandibular function and active and passive mouth opening showed no significant change over all time points. Conclusions: In this pragmatic cohort, BTX-A injections in the masseter and temporalis muscles seem to improve pain and oral health-related quality of life in patients with TMD-related myalgia within one month and show effects lasting up to six months, while mandibular function did not improve. Full article

Background/Objectives: For most adults receiving a cochlear implant (CI), the primary goal is to enhance their oral communication with others. The aim of this study was to investigate the total CI usage time per day among retired CI users and to characterize in which auditory environments they were using their CI. One additional aim was to analyze whether usage time, auditory environment, or social factors influenced CI speech perception. Methods: Participants completed a questionnaire addressing retirement status, whether they lived with another adult, educational level, and participation in social activities. Speech perception scores were obtained from medical records, and CI datalogging was extracted from the CI programming software. Results: Seventy-three CI users aged >65 years were included. The average usage was 12.9 h/day. No statistically significant correlations were found between total usage time or time spent listening to speech and CI speech perception. CI users who regularly met with family or friends had statistically significantly higher CI speech perception than those who did not (p = 0.003). Conclusions: Social interaction may play a crucial role in supporting speech perception among elderly CI users. Opportunities for communication and participation in social life appear to be important for maximizing benefit. Full article

Genetic truncation or mutation of the gene encoding band 4.1, ezrin, radixin, and moesin (FERM) domain protein containing 4A (FRMD4A) is associated with brain developmental diseases, including microcephaly with global developmental delay. It has also been identified as a risk factor for Alzheimer’s disease. By analogy with other FERM domain-containing proteins, FRMD4A is believed to regulate cell morphogenesis and/or cell polarization in central nervous system (CNS) cells; however, it remains unclear whether and how dysfunction of FRMD4A and/or its closely homologous protein FRMD4B causes abnormal morphogenesis in neuronal cells. Here, we describe for the first time the roles of FRMD4A and FRMD4B in process elongation in neuronal cells. Knockdown of Frmd4a or Frmd4b using specific RNA-targeting clustered regularly interspaced short palindromic repeat (CRISPR) and Cas13-fitted gRNAs led to decreased process elongation in primary cortical neurons. Similar decreases in neuronal marker expression were observed in the N1E-115 cell line, a model of neuronal differentiation. Furthermore, hesperetin, an aglycone of the citrus flavonoid hesperidin known to promote neuroprotective signaling, recovered the decreased process elongation induced by the knockdown of Frmd4a or Frm4b. Hesperetin also stimulated phosphorylation of mitogen-activated protein kinases/extracellular signal-regulated kinases (MAPKs/ERKs), which could help promote neuronal processes. These results suggest that FRMD4A and FRMD4B regulate process elongation through a possible signaling pathway linked to the sustained phosphorylation of MAPKs/ERKs. Crucially, this study reveals that, at the molecular and cellular levels, hesperetin can restore normal phenotypes when FRMD4A protein or FRMD4B protein is impaired. Full article

Background: Habitual breakfast skipping is associated with an increased risk of cardiovascular and cardiometabolic diseases. However, the effects of skipping breakfast regularly versus irregularly on vascular endothelial function (VEF), a key marker of cardiovascular health, remain unclear. This study aimed to investigate the effects of eight-Day regular or irregular breakfast skipping on brachial artery VEF in healthy habitual breakfast eaters using a three-condition, randomized controlled crossover trial. Methods: Ten young healthy adults (seven females, three males) completed three randomized nine-Day trials: (1) Eat (three meals per day), (2) Skip (breakfast skipped on days 1–8, consumed on Day 9), and (3) Eat/Skip (alternating breakfast consumption and skipping). Flow-mediated dilation (FMD) of the right brachial artery was assessed at 7:45–55 am on days 1, 2, 5, and 9, expressed as the percentage change in the brachial artery diameter normalized to the shear rate area under the curve (Δ%FMD/SR_AUC). Blood samples were collected before and 30 min after breakfast or lunch for glucose, insulin, free fatty acids, and triglyceride analyses. Insulin resistance was estimated using the homeostasis model assessment of insulin resistance calculated from fasting glucose and fasting insulin values. Objective measurements of sleep, physical activity, and continuous glucose monitoring were obtained. Results: On Day 9, the Skip and Eat/Skip trials had significantly lower %FMD/SR_AUC and significantly higher levels of fasting plasma insulin than the Eat trial. Exploratory analyses within the Skip and Eat/Skip trials suggested a weak negative association between changes in %FMD/SR_AUC and fasting blood glucose and insulin from day 1 to day 9. Conclusions: These findings suggest that both regular and irregular breakfast skipping may impair early morning VEF, possibly through alterations in glucose metabolism, whereas regular breakfast consumption may help preserve VEF and support cardiovascular health. Clinical Trial Registry: Clinical Trial Registry: University Hospital Medical Information Network (UMIN000053117, registered 20 December 2023). Full article

Urban year-round green exercise (YRGE)—defined as moderate to vigorous physical activity performed regularly in natural urban settings throughout all seasons and weather conditions—has the potential to promote health, well-being, and social connectedness. This study investigates the relationship between YRGE and individuals’ perceived health status, psychological well-being, and reasons for engagement. A cross-sectional online survey was conducted with 408 adult participants engaged in urban green exercise. The findings indicate that physical activity in adverse meteorological conditions, such as rain, cold, and wind, is positively associated with perceived current health, health over the past 12 months, and well-being. Social connectedness is particularly influenced by environmental factors like sun exposure and heat. The study also reveals key motivational factors for YRGE participation, including improving health and fitness, disconnecting from everyday life, enjoying nature, and experiencing tranquility, with significant variation depending on age and individual nature connectedness. These results suggest that YRGE serves as an accessible and inclusive public health intervention with consistent benefits across socio-demographic groups. Urban planning and health promotion initiatives should prioritize the maintenance and accessibility of urban green spaces and offer guided YRGE programs to encourage sustainable participation across the population. Full article

CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) nucleases enable precise genome editing, but off-target cleavage remains a critical challenge. Here, we report the development of MAD7_HF, a high-fidelity variant of the MAD7 nuclease engineered through a bacterial screening system leveraging the DNA gyrase-targeting toxic gene ccdB. This system couples survival to efficient on-target cleavage and minimal off-target activity, mimicking the transient action required for high-precision editing. Through iterative selection and sequencing validation, we identified MAD7_HF, harboring three substitutions (R187C, S350T, K1019N) that enhanced discrimination between on- and off-target sites. In Escherichia coli assays, MAD7_HF exhibited a >20-fold reduction in off-target cleavage across multiple mismatch contexts while maintaining on-target efficiency comparable to wild-type MAD7. Structural modeling revealed that these mutations stabilize the guide RNA-DNA hybrid at on-target sites and weaken interactions with mismatched sequences. This work establishes a high-throughput bacterial screening strategy that allows the identification of Cas12a variants with improved specificity at a given target site, providing a useful framework for future efforts to develop precision genome-editing tools. Full article

The C-BARQ familiar dog aggression (FDA) subscale contains four items relating to threatening responses towards familiar dogs in the same household (i.e., dog rivalry). In a recent study, we noticed that 92 of 157 guardians who owned only one dog completed the FDA items, generating an unexpected score. We followed up with participants to explore whether lifestyle factors influenced their completion of the FDA items. Singleton dogs with FDA scores were more likely to regularly participate in social activities with other dogs, with many scores based on such interactions with non-household dogs. The singleton dogs with FDA scores also had marginally lower fear-related C-BARQ scores compared to singletons with no FDA score and dogs living in multi-dog households. We then conducted a scoping review of articles using English versions of the C-BARQ and found wide variation in whether or not FDA scores were reported. Studies that reported significant FDA findings often did not indicate the proportion of scores in their data that came from singleton dogs, raising issues of accuracy and interpretation of the subscale. We discuss ways to clarify the interpretation of the FDA questions by dog guardians and hope to promote further consideration of practices to improve replicability across studies. Full article

Background: Chronic wrist pain is becoming increasingly recognized among athletes engaging in wrist-loading activities such as handstands. However, its prevalence and associated risk factors in handstand practitioners have not been systematically studied. This study aimed to investigate the prevalence of chronic wrist pain and to explore associated factors such as discipline, training habits, and pain management strategies. Methods: This cross-sectional study aimed to investigate the prevalence and associated factors of chronic wrist pain among handstand practitioners. Eligible participants were individuals aged 18 years or older, of any gender, who practiced handstands regularly (defined as at least once per week). Participants were recruited via a combination of open invitations on social media (Facebook, WhatsApp, Instagram) and direct outreach to movement studios and training communities. The survey was administered online using Google Forms and remained open for two months. Participation was voluntary and anonymous. Descriptive statistics were used to present sociodemographic characteristics, including age group, gender, sport discipline, and weekly training hours. Participants reported training habits, equipment use, pain history, and management strategies via a self-developed questionnaire designed for this study. Chronic pain was defined as recurring or persistent wrist pain. Descriptive statistics were used to summarize responses. Associations between chronic wrist pain and survey variables were analyzed using Chi-square or Fisher’s exact tests for nominal data, and Chi-square test for trend for ordinal data. A p-value < 0.05 was considered statistically significant. Results: A total of 321 participants were included in the study. The most represented age group was 25–34 years, comprising 123 (38.3%) of the participants. Gender distribution was 174 (54.2%) males and 147 (45.8%) females. The most common sport disciplines were Yoga (88, 27.4%), Capoeira (60, 18.7%), and Movement (52, 16.2%). Chronic wrist pain was reported by 182 (56.7%) of participants. Younger age was significantly associated with higher pain prevalence (p = 0.042). No significant associations were observed between chronic pain and weekly training hours, warm-up routines, brace use, or grip device use. Female participants demonstrated more proactive pain management behaviors (p = 0.016). Sport discipline and training practices showed non-significant trends toward pain differences. Conclusions: Chronic wrist pain is common among handstand practitioners, particularly among younger athletes. These findings suggest that injury risk may relate more to training intensity and biomechanics than to simple training volume. Further research incorporating objective diagnostics and standardized intervention protocols is warranted. Full article

Streptococcus thermophilus is a Gram-positive, homofermentative lactic acid bacterium classified within the Firmicutes phylum, recognized for its probiotic properties and significant role in promoting human health. This review consolidates existing understanding of its metabolic pathways, functional metabolites, and diverse applications, highlighting evidence-based insights to enhance scientific integrity. S. thermophilus predominantly ferments lactose through the Embden-Meyerhof-Parnas pathway, resulting in L(+)-lactic acid as the primary end-product, along with secondary metabolites including acetic acid, formic acid, and pyruvate derivatives. Exopolysaccharides (EPS) are composed of repeating units of glucose, galactose, rhamnose, and N-acetylgalactosamine. They display strain-specific molecular weights ranging from 10 to 2000 kDa and contribute to the viscosity of fermented products, while also providing antioxidant and immunomodulatory benefits. Aromatic compounds such as acetaldehyde and phenylacetic acid are products of amino acid catabolism and carbohydrate metabolism, playing a significant role in the sensory characteristics observed in dairy fermentations. Bacteriocins, such as thermophilins (e.g., Thermophilin 13, 110), exhibit extensive antimicrobial efficacy against pathogens including Listeria monocytogenes and Bacillus cereus. Their activity is modulated by quorum-sensing mechanisms that involve the blp gene cluster, and they possess significant stability under heat and pH variations, making them suitable for biopreservation applications. In food applications, S. thermophilus functions as a Generally Recognized as Safe (GRAS) starter culture in the production of yogurt and cheese, working in conjunction with Lactobacillus delbrueckii subsp. bulgaricus to enhance acidification and improve texture. Specific strains have been identified to mitigate lactose intolerance, antibiotic-related diarrhea, and inflammatory bowel diseases through the modulation of gut microbiota, the production of short-chain fatty acids, and the inhibition of Helicobacter pylori. The genome, characterized by a G + C content of approximately 37 mol%, facilitates advancements in Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas technology and heterologous protein expression, with applications extending to non-dairy fermentations and the development of postbiotics. This review emphasizes the adaptability of S. thermophilus, showcasing the variability among strains and the necessity for thorough preclinical and clinical validation to fully utilize its potential in health, sustainable agriculture, and innovation. It also addresses challenges such as susceptibility to bacteriophages and limitations in proteolytic activity. Full article

The integration of Artificial Intelligence into education is transforming how abstract and complex concepts are delivered, especially through embodied tools like social robots. This study examines the impact of AlphaMini, a Slovenian-speaking social robot supported by model ChatGPT 4o and trained on structured book-based content, on student engagement during knowledge management lessons. A case study approach was used, including student questionnaires, classroom observations, and post-session discussions, with 70 university students from diverse academic fields. Engagement was assessed across behavioral, emotional, and cognitive dimensions, with comparisons based on prior robot experience. Results show AlphaMini significantly enhanced emotional and behavioral engagement, with moderate cognitive gains. Students familiar with social robots demonstrated higher engagement, interacting more naturally and actively. Informal feedback highlighted positive attitudes toward AlphaMini, especially among students who regularly use generative AI tools like ChatGPT or Copilot. Participants appreciated its human-like gestures, Slovenian language use, and emotionally supportive presence. Many suggested its potential use in primary and inclusive education, where emotional safety and playful interaction are crucial. This study contributes to the growing evidence on AI in education, showing that combining generative AI with social robotics can foster motivation, participation, and emotionally rich learning experiences. Full article

Background: Physical inactivity and unhealthy dietary habits are among the major global public health concerns, contributing significantly to the increasing prevalence of non-communicable diseases. Objectives: The present study aimed to investigate the relationship between physical activity (PA) and dietary choices among undergraduate university students in Southern Italy. Methods: The cross-sectional survey was carried out through an anonymous web-based questionnaire. Results: Among the 500 university students who agreed to participate, only 3.4% of students reported regularly consuming five or more portions of fruits and vegetables per day, while 43.8% consumed 3–5 portions of starchy foods. Regarding fats, 31.2% reported consuming 2–3 portions of olive oil or butter per day. Men and those not having a health problem in the previous 12 months were less likely to adhere to the World Health Organization (WHO) recommendations on PA. Older students, those who consumed at least 5 meals per day, and those who acquired information from at least one source of information were more likely to adhere to the WHO recommendations on PA. Conclusions: Targeted initiatives promoting regular PA and healthy diets are essential to improving students’ health and well-being. Full article

Thermal algae are extremophilic organisms that live in one of the harshest environments in the world and thrive in waters with temperatures of up to 90 °C. They have gained attention due to their special ecological adaptations, their great biotechnological potential and their recently recognised role in combating global climate change and achieving sustainable development. However, the biodiversity of these algae is far from being fully explored. The article presents the first finding of the prokaryotic genus Asterocapsa (Chroococcales, Cyanophyceae, Cyanoprokaryota/Cyanobacteria) in thermal waters and describes a new species from the fountain basins in the thermal system of the only continental European geyser (101 °C) in the town of Sapareva Banya (south-west Bulgaria). This species is not only one of the few aquatic representatives of this generally aeroterrestrial genus, but is also characterised by its extremophilic lifestyle and differs clearly from the type species and other aquatic species of the genus due to its morphological characteristics. These include the smaller dimensions of the cells and colonies, as well as the colourless, transparent, but always lamellar and regularly verrucous mucilage envelopes. The unique locality of this alga is highly endangered and was included in the first Red List of Bulgarian wetlands. Due to human activities and changes in the geyser system, we have detected some unfavourable changes in the algal habitat and therefore propose to add the newly described species to the Red List of Bulgarian Microalgae with the status Critically Endangered. Full article

The increasing contradiction between ecological conservation and community development is a common challenge faced in most protected areas worldwide. Since 2019, China has used a “sky window” policy to alleviate the dilemma of environmental protection and sustainable production activities in national parks. This policy’s impact on household income in national park communities has received little attention. In this study, we aimed to evaluate the impact of the sky window policy on household income in Wuyi Mountain National Park communities in China and explore its mechanism of action in order to provide policy recommendations for achieving the protection goal of the national park and enabling win–win development of the community. Based on a total of 951 samples, which were collected through face-to-face interviews with 518 households in two periods, we used the difference-in-differences (DID) model to obtain consistent results and conducted robustness tests on the model by employing propensity score matching (PSM). The results showed that the “sky window” policy had a significant negative impact on the income of households in national park communities, which was mainly caused by the relaxation of restrictive regulations on farmers’ planting and breeding activities within national parks. The findings indicate that government departments in China need to further improve the laws and regulations regarding national park construction, establish a dynamic evaluation mechanism to regularly review the effects of the “sky window” policy, and make timely adjustments based on changes in the ecological environment of national parks and the development needs of local communities. At the same time, to ensure a stable source of income for residents, it is also necessary to establish a platform for realizing the value of ecological products, strengthen support for livelihood transformation, and establish long-term benefit linkage mechanisms. This study contributes to the research on the effective management of national parks, community welfare improvement, and sustainable development in developing countries. Full article

Fms-like tyrosine kinase 3 (FLT3) is a receptor tyrosine kinase (RTK) that is involved in cell survival, proliferation, and differentiation of haematopoietic progenitors of lymphoid and myeloid lineages. Oncogenic mutations in the FLT3 gene, resulting in constitutively active FLT3 variants, are frequently found in patients with acute myeloid leukaemia (AML). In particular, patients expressing FLT3 ITD (internal tandem duplications of the juxtamembrane domain of FLT3) correlate with poor patient survival. Targeting FLT3-mutated leukaemic stem cells is therefore a key to the efficient treatment of patients with relapsed/refractory AML. The efficacy of approved tyrosine kinase inhibitors is regularly compromised by various resistance pathways or secondary mutations. Based on the current molecular understanding of aberrant signal transduction pathways and cell transformation, novel alternative treatment approaches can be exploited for therapeutic purposes. In particular, new insights into the regulation of the activity of counteracting protein tyrosine phosphatases (PTPs), the aberrant biogenesis and activation of mutant FLT3 proteins, as well as common factors controlling cell transformation are attractive avenues. This review summarises the current knowledge about the regulation of the oncogenic activities of mutant FLT3 proteins and discusses possible options for alternative treatments. Full article

Playgrounds are global environments that are purpose made for children and can offer a variety of opportunities for children to be physically active and practice their fundamental movement skills (FMS), which can lead to future physical activity and sport participation. Previous research highlighted that children engage in different types of physical activity (PA) depending on playgrounds apparatus and area. However, there is a paucity of research that investigates the link between playground features, structures, PA, and FMS. This study sought to assess the impact of different playgrounds on PA type PA intensity and the types of FMS completed. This observational study examined 29 (M = 10, F = 19) children’s behaviours on three different playgrounds. Video cameras were placed strategically across the three playgrounds to allow for footage to be captured and analysed using the Observational System for Recording Physical Activity in Children (OSRAC). One-way ANOVA was used to examine the different OSRAC categories across the three playgrounds. Climbing equipment (average 1217.10 s) was the frequently used type of apparatus, standing was the most commonly performed type of activity (average 377.60 s) and stationary movements whilst moving limbs were the most regularly (average 605.13 s) performed type of PA intensity. There were no instances of any throwing, catching, or kicking activities performed across the three playgrounds. Results suggest that public playgrounds do not facilitate more intense types of PA, nor object control skills due to a lack of suitable equipment. Full article